Restriction map for
pF1KE2329
(Linear) (Six-base) MAPSORT of: KIEE2329.seq Check: 2348 from: 1 to: 3939
KIEE2329.orf
With 182 enzymes: SgfI *
February 23, 2011 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1117 3627 3939
Size: 1117 2510 312
Fragments arranged by size:
2510 1117 312
AccI GT'mk_AC
Cuts at: 0 763 1237 1264 3709 3939
Size: 763 474 27 2445 230
Fragments arranged by size:
2445 763 474 230 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1580 1832 2171 3254 3431 3893 3939
Size: 508 1072 252 339 1083 177 462 46
Fragments arranged by size:
1083 1072 508 462 339 252 177 46
AhdI GACnn_n'nnGTC
Cuts at: 0 2635 3939
Size: 2635 1304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3131 3320 3939
Size: 3131 189 619
Fragments arranged by size:
3131 619 189
AlwNI CAG_nnn'CTG
Cuts at: 0 1819 2552 3939
Size: 1819 733 1387
Fragments arranged by size:
1819 1387 733
ApaI G_GGCC'C
Cuts at: 0 473 3073 3939
Size: 473 2600 866
Fragments arranged by size:
2600 866 473
ApaLI G'TGCA_C
Cuts at: 0 3851 3939
Size: 3851 88
ApoI r'AATT_y
Cuts at: 0 1297 1428 1533 1611 1942 2395 3939
Size: 1297 131 105 78 331 453 1544
Fragments arranged by size:
1544 1297 453 331 131 105 78
AscI GG'CGCG_CC
Cuts at: 0 421 3939
Size: 421 3518
AvaI C'yCGr_G
Cuts at: 0 76 138 339 1367 1727 2004 3939
Size: 76 62 201 1028 360 277 1935
Fragments arranged by size:
1935 1028 360 277 201 76 62
BamHI G'GATC_C
Cuts at: 0 1188 3939
Size: 1188 2751
BanI G'GyrC_C
Cuts at: 0 343 382 3231 3939
Size: 343 39 2849 708
Fragments arranged by size:
2849 708 343 39
BanII G_rGCy'C
Cuts at: 0 205 473 1118 1567 2926 3073 3291 3382
Size: 205 268 645 449 1359 147 218 91
Cuts at: 3382 3939
Size: 557
Fragments arranged by size:
1359 645 557 449 268 218 205 147
91
BbeI G_GCGC'C
Cuts at: 0 347 386 3939
Size: 347 39 3553
Fragments arranged by size:
3553 347 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1838 3939
Size: 1838 2101
BbvCI CC'TCA_GC
Cuts at: 0 3128 3440 3939
Size: 3128 312 499
Fragments arranged by size:
3128 499 312
BciVI GTATCCnnnnn_n'
Cuts at: 0 158 1959 2918 3939
Size: 158 1801 959 1021
Fragments arranged by size:
1801 1021 959 158
BclI T'GATC_A
Cuts at: 0 2492 3939
Size: 2492 1447
BfrBI ATG'CAT
Cuts at: 0 3316 3939
Size: 3316 623
BglI GCCn_nnn'nGGC
Cuts at: 0 81 105 352 1758 3538 3939
Size: 81 24 247 1406 1780 401
Fragments arranged by size:
1780 1406 401 247 81 24
BlpI GC'TnA_GC
Cuts at: 0 6 2717 3389 3939
Size: 6 2711 672 550
Fragments arranged by size:
2711 672 550 6
Bme1580I G_kGCm'C
Cuts at: 0 473 1274 1356 1451 3073 3855 3939
Size: 473 801 82 95 1622 782 84
Fragments arranged by size:
1622 801 782 473 95 84 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 1350 1958 2099 2911 3497 3939
Size: 1350 608 141 812 586 442
Fragments arranged by size:
1350 812 608 586 442 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 224 3939
Size: 224 3715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1017 1132 1740 1796 1967 2849 3939
Size: 76 941 115 608 56 171 882 1090
Fragments arranged by size:
1090 941 882 608 171 115 76 56
Bpu10I CC'TnA_GC
Cuts at: 0 2519 3128 3440 3939
Size: 2519 609 312 499
Fragments arranged by size:
2519 609 499 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 2804 3939
Size: 1174 1630 1135
Fragments arranged by size:
1630 1174 1135
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 660 2078 3939
Size: 292 368 1418 1861
Fragments arranged by size:
1861 1418 368 292
BsaAI yAC'GTr
Cuts at: 0 1241 3735 3939
Size: 1241 2494 204
Fragments arranged by size:
2494 1241 204
BsaHI Gr'CG_yC
Cuts at: 0 344 383 410 2959 3939
Size: 344 39 27 2549 980
Fragments arranged by size:
2549 980 344 39 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 648 672 1970 3939
Size: 11 637 24 1298 1969
Fragments arranged by size:
1969 1298 637 24 11
BseYI C'CCAG_C
Cuts at: 0 494 1880 1912 2584 2667 3054 3248 3654
Size: 494 1386 32 672 83 387 194 406
Cuts at: 3654 3939
Size: 285
Fragments arranged by size:
1386 672 494 406 387 285 194 83
32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3403 3482 3939
Size: 3403 79 457
Fragments arranged by size:
3403 457 79
BsiEI CG_ry'CG
Cuts at: 0 14 100 356 1750 3939
Size: 14 86 256 1394 2189
Fragments arranged by size:
2189 1394 256 86 14
BsiHKAI G_wGCw'C
Cuts at: 0 205 2621 3855 3939
Size: 205 2416 1234 84
Fragments arranged by size:
2416 1234 205 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 417 1793 3939
Size: 417 1376 2146
Fragments arranged by size:
2146 1376 417
Bsp1286I G_dGCh'C
Cuts at: 0 205 473 1118 1274 1356 1451 1567 2621
Size: 205 268 645 156 82 95 116 1054
Cuts at: 2621 2926 3073 3291 3382 3855 3939
Size: 305 147 218 91 473 84
Fragments arranged by size:
1054 645 473 305 268 218 205 156
147 116 95 91 84 82
BspHI T'CATG_A
Cuts at: 0 3291 3939
Size: 3291 648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1117 3627 3939
Size: 1117 2510 312
Fragments arranged by size:
2510 1117 312
BsrBI CCG'CTC
Cuts at: 0 113 378 3939
Size: 113 265 3561
Fragments arranged by size:
3561 265 113
BsrFI r'CCGG_y
Cuts at: 0 99 459 3702 3939
Size: 99 360 3243 237
Fragments arranged by size:
3243 360 237 99
BsrGI T'GTAC_A
Cuts at: 0 3750 3939
Size: 3750 189
BssHII G'CGCG_C
Cuts at: 0 421 3939
Size: 421 3518
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3484 3939
Size: 3484 455
BstYI r'GATC_y
Cuts at: 0 1188 1579 3939
Size: 1188 391 2360
Fragments arranged by size:
2360 1188 391
BstZ17I GTA'TAC
Cuts at: 0 1265 3710 3939
Size: 1265 2445 229
Fragments arranged by size:
2445 1265 229
Bsu36I CC'TnA_GG
Cuts at: 0 2633 3740 3939
Size: 2633 1107 199
Fragments arranged by size:
2633 1107 199
BtgI C'CryG_G
Cuts at: 0 32 572 671 3374 3939
Size: 32 540 99 2703 565
Fragments arranged by size:
2703 565 540 99 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1643 3322 3939
Size: 1643 1679 617
Fragments arranged by size:
1679 1643 617
DraI TTT'AAA
Cuts at: 0 882 1020 1304 2390 3939
Size: 882 138 284 1086 1549
Fragments arranged by size:
1549 1086 882 284 138
DraIII CAC_nnn'GTG
Cuts at: 0 1966 3939
Size: 1966 1973
EaeI y'GGCC_r
Cuts at: 0 11 38 97 353 668 1654 1747 1771
Size: 11 27 59 256 315 986 93 24
Cuts at: 1771 3551 3939
Size: 1780 388
Fragments arranged by size:
1780 986 388 315 256 93 59 27
24 11
EagI C'GGCC_G
Cuts at: 0 11 97 353 1747 3939
Size: 11 86 256 1394 2192
Fragments arranged by size:
2192 1394 256 86 11
EarI CTCTTCn'nnn_
Cuts at: 0 1724 2693 3939
Size: 1724 969 1246
Fragments arranged by size:
1724 1246 969
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 123 144 204 3939
Size: 123 21 60 3735
Fragments arranged by size:
3735 123 60 21
EcoICRI GAG'CTC
Cuts at: 0 203 3939
Size: 203 3736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 508 1017 1132 1580 1740 1796 1832
Size: 76 432 509 115 448 160 56 36
Cuts at: 1832 1967 2171 2849 3254 3431 3893 3939
Size: 135 204 678 405 177 462 46
Fragments arranged by size:
678 509 462 448 432 405 204 177
160 135 115 76 56 46 36
EcoO109I rG'GnC_Cy
Cuts at: 0 470 1107 1708 1760 1855 2629 3939
Size: 470 637 601 52 95 774 1310
Fragments arranged by size:
1310 774 637 601 470 95 52
EcoRI G'AATT_C
Cuts at: 0 1533 1611 3939
Size: 1533 78 2328
Fragments arranged by size:
2328 1533 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1070 1601 2447 3371 3939
Size: 1070 531 846 924 568
Fragments arranged by size:
1070 924 846 568 531
FspI TGC'GCA
Cuts at: 0 3401 3939
Size: 3401 538
FspAI rTGC'GCAy
Cuts at: 0 3401 3939
Size: 3401 538
HaeII r_GCGC'y
Cuts at: 0 87 347 386 3939
Size: 87 260 39 3553
Fragments arranged by size:
3553 260 87 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 2477 2509 2621 2653 3939
Size: 1015 32 1430 32 112 32 1286
Fragments arranged by size:
1430 1286 1015 112 32 32 32
KasI G'GCGC_C
Cuts at: 0 343 382 3939
Size: 343 39 3557
Fragments arranged by size:
3557 343 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2170 3650 3802 3939
Size: 2170 1480 152 137
Fragments arranged by size:
2170 1480 152 137
MscI TGG'CCA
Cuts at: 0 40 1656 3553 3939
Size: 40 1616 1897 386
Fragments arranged by size:
1897 1616 386 40
MslI CAynn'nnrTG
Cuts at: 0 2968 3038 3188 3939
Size: 2968 70 150 751
Fragments arranged by size:
2968 751 150 70
MspA1I CmG'CkG
Cuts at: 0 10 34 96 574 605 2176 2716 3018
Size: 10 24 62 478 31 1571 540 302
Cuts at: 3018 3067 3939
Size: 49 872
Fragments arranged by size:
1571 872 540 478 302 62 49 31
24 10
NaeI GCC'GGC
Cuts at: 0 101 3704 3939
Size: 101 3603 235
Fragments arranged by size:
3603 235 101
NarI GG'CG_CC
Cuts at: 0 344 383 3939
Size: 344 39 3556
Fragments arranged by size:
3556 344 39
NgoMIV G'CCGG_C
Cuts at: 0 99 3702 3939
Size: 99 3603 237
Fragments arranged by size:
3603 237 99
NotI GC'GGCC_GC
Cuts at: 0 11 3939
Size: 11 3928
NsiI A_TGCA'T
Cuts at: 0 3318 3939
Size: 3318 621
NspI r_CATG'y
Cuts at: 0 1845 2675 3163 3307 3320 3939
Size: 1845 830 488 144 13 619
Fragments arranged by size:
1845 830 619 488 144 13
PasI CC'CwG_GG
Cuts at: 0 1118 3939
Size: 1118 2821
PfoI T'CCnGG_A
Cuts at: 0 1485 3939
Size: 1485 2454
PpuMI rG'GwC_Cy
Cuts at: 0 1107 1708 2629 3939
Size: 1107 601 921 1310
Fragments arranged by size:
1310 1107 921 601
PshAI GACnn'nnGTC
Cuts at: 0 1785 2070 3939
Size: 1785 285 1869
Fragments arranged by size:
1869 1785 285
PspOMI G'GGCC_C
Cuts at: 0 469 3069 3939
Size: 469 2600 870
Fragments arranged by size:
2600 870 469
PspXI vC'TCGA_Gb
Cuts at: 0 1367 3939
Size: 1367 2572
PstI C_TGCA'G
Cuts at: 0 2280 3939
Size: 2280 1659
PvuII CAG'CTG
Cuts at: 0 2176 2716 3939
Size: 2176 540 1223
Fragments arranged by size:
2176 1223 540
SacI G_AGCT'C
Cuts at: 0 205 3939
Size: 205 3734
SacII CC_GC'GG
Cuts at: 0 35 575 3939
Size: 35 540 3364
Fragments arranged by size:
3364 540 35
SbfI CC_TGCA'GG
Cuts at: 0 2280 3939
Size: 2280 1659
SfcI C'TryA_G
Cuts at: 0 2276 3120 3195 3856 3939
Size: 2276 844 75 661 83
Fragments arranged by size:
2276 844 661 83 75
SfoI GGC'GCC
Cuts at: 0 345 384 3939
Size: 345 39 3555
Fragments arranged by size:
3555 345 39
SmaI CCC'GGG
Cuts at: 0 78 140 341 3939
Size: 78 62 201 3598
Fragments arranged by size:
3598 201 78 62
SmlI C'TyrA_G
Cuts at: 0 1153 1367 2819 3939
Size: 1153 214 1452 1120
Fragments arranged by size:
1452 1153 1120 214
SnaBI TAC'GTA
Cuts at: 0 1241 3939
Size: 1241 2698
SphI G_CATG'C
Cuts at: 0 3307 3939
Size: 3307 632
SrfI GCCC'GGGC
Cuts at: 0 341 3939
Size: 341 3598
SspI AAT'ATT
Cuts at: 0 2295 3939
Size: 2295 1644
StyI C'CwwG_G
Cuts at: 0 282 416 1861 2646 3939
Size: 282 134 1445 785 1293
Fragments arranged by size:
1445 1293 785 282 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 1977 3068 3437 3516 3939
Size: 642 1335 1091 369 79 423
Fragments arranged by size:
1335 1091 642 423 369 79
TatI w'GTAC_w
Cuts at: 0 843 934 1358 3750 3912 3939
Size: 843 91 424 2392 162 27
Fragments arranged by size:
2392 843 424 162 91 27
XhoI C'TCGA_G
Cuts at: 0 1367 3939
Size: 1367 2572
XmaI C'CCGG_G
Cuts at: 0 76 138 339 3939
Size: 76 62 201 3600
Fragments arranged by size:
3600 201 76 62
XmnI GAAnn'nnTTC
Cuts at: 0 3649 3939
Size: 3649 290
Enzymes that do cut:
AarI AccI AcuI AhdI AlfI AlwNI ApaI ApaLI
ApoI AscI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NsiI NspI PasI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SbfI SfcI
SfoI SmaI SmlI SnaBI SphI SrfI SspI StyI
TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI AseI AsiSI AvrII BaeI BcgI BglII BmgBI
BmtI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BsrDI
BssSI BstAPI BstBI BstEII BtsI ClaI CspCI DrdI
EcoNI EcoRV FseI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NruI PacI PciI PflMI
PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SpeI
StuI SwaI TstI Tth111I XbaI XcmI ZraI