Restriction map for
pF1KE2332
(Linear) (Six-base) MAPSORT of: KIEE2332.seq Check: 1982 from: 1 to: 1653
KIEE2332.orf
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AccI GT'mk_AC
Cuts at: 0 769 1653
Size: 769 884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 472 632 1653
Size: 260 212 160 1021
Fragments arranged by size:
1021 260 212 160
AflII C'TTAA_G
Cuts at: 0 1243 1653
Size: 1243 410
AflIII A'CryG_T
Cuts at: 0 553 888 1648 1653
Size: 553 335 760 5
Fragments arranged by size:
760 553 335 5
AhdI GACnn_n'nnGTC
Cuts at: 0 528 1653
Size: 528 1125
ApoI r'AATT_y
Cuts at: 0 151 270 645 1099 1653
Size: 151 119 375 454 554
Fragments arranged by size:
554 454 375 151 119
AseI AT'TA_AT
Cuts at: 0 814 1228 1653
Size: 814 414 425
Fragments arranged by size:
814 425 414
BanI G'GyrC_C
Cuts at: 0 729 1653
Size: 729 924
BanII G_rGCy'C
Cuts at: 0 1369 1653
Size: 1369 284
BclI T'GATC_A
Cuts at: 0 945 1653
Size: 945 708
BglII A'GATC_T
Cuts at: 0 721 1239 1653
Size: 721 518 414
Fragments arranged by size:
721 518 414
Bpu10I CC'TnA_GC
Cuts at: 0 822 1653
Size: 822 831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1653
Size: 1042 611
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 997 1653
Size: 697 300 656
Fragments arranged by size:
697 656 300
BsaAI yAC'GTr
Cuts at: 0 554 1653
Size: 554 1099
BsaBI GATnn'nnATC
Cuts at: 0 995 1037 1653
Size: 995 42 616
Fragments arranged by size:
995 616 42
Bsp1286I G_dGCh'C
Cuts at: 0 1369 1653
Size: 1369 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 1653
Size: 10 1643
BstAPI GCAn_nnn'nTGC
Cuts at: 0 193 1653
Size: 193 1460
BstYI r'GATC_y
Cuts at: 0 721 1239 1653
Size: 721 518 414
Fragments arranged by size:
721 518 414
BstZ17I GTA'TAC
Cuts at: 0 770 1653
Size: 770 883
Bsu36I CC'TnA_GG
Cuts at: 0 1568 1653
Size: 1568 85
BtgI C'CryG_G
Cuts at: 0 290 1653
Size: 290 1363
BtsI GCAGTG_nn'
Cuts at: 0 1035 1457 1653
Size: 1035 422 196
Fragments arranged by size:
1035 422 196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1184 1219 1653
Size: 1184 35 434
Fragments arranged by size:
1184 434 35
DraI TTT'AAA
Cuts at: 0 801 1653
Size: 801 852
EaeI y'GGCC_r
Cuts at: 0 467 834 1653
Size: 467 367 819
Fragments arranged by size:
819 467 367
EarI CTCTTCn'nnn_
Cuts at: 0 141 1137 1544 1653
Size: 141 996 407 109
Fragments arranged by size:
996 407 141 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 928 1653
Size: 928 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 472 632 1653
Size: 260 212 160 1021
Fragments arranged by size:
1021 260 212 160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 95 1653
Size: 95 1558
EcoO109I rG'GnC_Cy
Cuts at: 0 100 1115 1601 1653
Size: 100 1015 486 52
Fragments arranged by size:
1015 486 100 52
EcoRI G'AATT_C
Cuts at: 0 270 1099 1653
Size: 270 829 554
Fragments arranged by size:
829 554 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 590 1653
Size: 590 1063
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1653
Size: 514 32 1107
Fragments arranged by size:
1107 514 32
MfeI C'AATT_G
Cuts at: 0 463 1653
Size: 463 1190
MscI TGG'CCA
Cuts at: 0 836 1653
Size: 836 817
MslI CAynn'nnrTG
Cuts at: 0 968 1093 1451 1653
Size: 968 125 358 202
Fragments arranged by size:
968 358 202 125
NcoI C'CATG_G
Cuts at: 0 290 1653
Size: 290 1363
NspI r_CATG'y
Cuts at: 0 1652 1653
Size: 1652 1
PciI A'CATG_T
Cuts at: 0 1648 1653
Size: 1648 5
PpuMI rG'GwC_Cy
Cuts at: 0 100 1115 1601 1653
Size: 100 1015 486 52
Fragments arranged by size:
1015 486 100 52
PsiI TTA'TAA
Cuts at: 0 136 398 1653
Size: 136 262 1255
Fragments arranged by size:
1255 262 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 944 976 1653
Size: 944 32 677
Fragments arranged by size:
944 677 32
ScaI AGT'ACT
Cuts at: 0 71 1653
Size: 71 1582
SfcI C'TryA_G
Cuts at: 0 1543 1653
Size: 1543 110
SmlI C'TyrA_G
Cuts at: 0 1021 1243 1653
Size: 1021 222 410
Fragments arranged by size:
1021 410 222
SspI AAT'ATT
Cuts at: 0 957 1653
Size: 957 696
StuI AGG'CCT
Cuts at: 0 308 1653
Size: 308 1345
StyI C'CwwG_G
Cuts at: 0 290 1118 1653
Size: 290 828 535
Fragments arranged by size:
828 535 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 747 1653
Size: 747 906
TatI w'GTAC_w
Cuts at: 0 69 1653
Size: 69 1584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1388 1420 1653
Size: 1388 32 233
Fragments arranged by size:
1388 233 32
XmnI GAAnn'nnTTC
Cuts at: 0 389 1442 1653
Size: 389 1053 211
Fragments arranged by size:
1053 389 211
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI ApoI AseI BanI
BanII BclI BglII Bpu10I BpuEI BsaI BsaAI BsaBI
Bsp1286I BspMI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI
CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I MfeI MscI MslI NcoI NspI
PciI PpuMI PsiI PsrI ScaI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BtgZI ClaI
DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI