Restriction map for
pF1KE2335
(Linear) (Six-base) MAPSORT of: KIEE2335.seq Check: 5621 from: 1 to: 4440
KIEE2335.orf
With 182 enzymes: SgfI *
May 11, 2017 11:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2896 4440
Size: 2896 1544
AccI GT'mk_AC
Cuts at: 0 1685 2802 4440
Size: 1685 1117 1638
Fragments arranged by size:
1685 1638 1117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1272 1415 2266 2778 3155 3809 4440
Size: 1272 143 851 512 377 654 631
Fragments arranged by size:
1272 851 654 631 512 377 143
AflII C'TTAA_G
Cuts at: 0 861 4440
Size: 861 3579
AflIII A'CryG_T
Cuts at: 0 2894 3588 4440
Size: 2894 694 852
Fragments arranged by size:
2894 852 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1051 4440
Size: 1051 3389
AlwNI CAG_nnn'CTG
Cuts at: 0 93 2224 4287 4440
Size: 93 2131 2063 153
Fragments arranged by size:
2131 2063 153 93
ApoI r'AATT_y
Cuts at: 0 568 1460 1664 1974 2094 2128 2837 3246
Size: 568 892 204 310 120 34 709 409
Cuts at: 3246 3582 4440
Size: 336 858
Fragments arranged by size:
892 858 709 568 409 336 310 204
120 34
AseI AT'TA_AT
Cuts at: 0 209 1331 1849 2995 4440
Size: 209 1122 518 1146 1445
Fragments arranged by size:
1445 1146 1122 518 209
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1301 1334 4440
Size: 1301 33 3106
Fragments arranged by size:
3106 1301 33
BamHI G'GATC_C
Cuts at: 0 1372 3912 4128 4440
Size: 1372 2540 216 312
Fragments arranged by size:
2540 1372 312 216
BanI G'GyrC_C
Cuts at: 0 1498 4440
Size: 1498 2942
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 2486 4440
Size: 493 1993 1954
Fragments arranged by size:
1993 1954 493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4080 4114 4440
Size: 4080 34 326
Fragments arranged by size:
4080 326 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 73 1720 4440
Size: 73 1647 2720
Fragments arranged by size:
2720 1647 73
BclI T'GATC_A
Cuts at: 0 2261 3933 4440
Size: 2261 1672 507
Fragments arranged by size:
2261 1672 507
BglI GCCn_nnn'nGGC
Cuts at: 0 2395 4440
Size: 2395 2045
BglII A'GATC_T
Cuts at: 0 3639 3973 4072 4094 4440
Size: 3639 334 99 22 346
Fragments arranged by size:
3639 346 334 99 22
BlpI GC'TnA_GC
Cuts at: 0 921 4440
Size: 921 3519
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 4109 4440
Size: 313 3796 331
Fragments arranged by size:
3796 331 313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 29 366 1605 4440
Size: 29 337 1239 2835
Fragments arranged by size:
2835 1239 337 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1400 1444 2090 3429 4440
Size: 255 1145 44 646 1339 1011
Fragments arranged by size:
1339 1145 1011 646 255 44
Bpu10I CC'TnA_GC
Cuts at: 0 4036 4440
Size: 4036 404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2423 4347 4440
Size: 2423 1924 93
Fragments arranged by size:
2423 1924 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 1984 3973 4440
Size: 1984 1989 467
Fragments arranged by size:
1989 1984 467
BsaAI yAC'GTr
Cuts at: 0 2757 3591 4440
Size: 2757 834 849
Fragments arranged by size:
2757 849 834
BsaHI Gr'CG_yC
Cuts at: 0 661 4440
Size: 661 3779
BsaWI w'CCGG_w
Cuts at: 0 998 4355 4440
Size: 998 3357 85
Fragments arranged by size:
3357 998 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 1219 2651 4314 4420 4440
Size: 619 600 1432 1663 106 20
Fragments arranged by size:
1663 1432 619 600 106 20
BseYI C'CCAG_C
Cuts at: 0 417 712 754 827 4440
Size: 417 295 42 73 3613
Fragments arranged by size:
3613 417 295 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1982 4440
Size: 1982 2458
BsiHKAI G_wGCw'C
Cuts at: 0 4122 4440
Size: 4122 318
BsmI GAATG_Cn'
Cuts at: 0 452 4231 4440
Size: 452 3779 209
Fragments arranged by size:
3779 452 209
Bsp1286I G_dGCh'C
Cuts at: 0 4122 4440
Size: 4122 318
BspEI T'CCGG_A
Cuts at: 0 998 4440
Size: 998 3442
BspHI T'CATG_A
Cuts at: 0 3416 4440
Size: 3416 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2896 4440
Size: 2896 1544
BsrDI GCAATG_nn'
Cuts at: 0 845 3332 3424 4223 4440
Size: 845 2487 92 799 217
Fragments arranged by size:
2487 845 799 217 92
BsrGI T'GTAC_A
Cuts at: 0 1071 4440
Size: 1071 3369
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3808 4440
Size: 3808 632
BstYI r'GATC_y
Cuts at: 0 1372 3639 3912 3973 4072 4094 4128 4440
Size: 1372 2267 273 61 99 22 34 312
Fragments arranged by size:
2267 1372 312 273 99 61 34 22
BstZ17I GTA'TAC
Cuts at: 0 1686 4440
Size: 1686 2754
Bsu36I CC'TnA_GG
Cuts at: 0 2393 3615 4440
Size: 2393 1222 825
Fragments arranged by size:
2393 1222 825
BtgI C'CryG_G
Cuts at: 0 4041 4440
Size: 4041 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 237 4440
Size: 237 4203
BtsI GCAGTG_nn'
Cuts at: 0 403 2531 2717 3283 3436 4440
Size: 403 2128 186 566 153 1004
Fragments arranged by size:
2128 1004 566 403 186 153
ClaI AT'CG_AT
Cuts at: 0 3806 4440
Size: 3806 634
DraI TTT'AAA
Cuts at: 0 494 1829 2061 4440
Size: 494 1335 232 2379
Fragments arranged by size:
2379 1335 494 232
DraIII CAC_nnn'GTG
Cuts at: 0 1645 3196 4440
Size: 1645 1551 1244
Fragments arranged by size:
1645 1551 1244
EaeI y'GGCC_r
Cuts at: 0 3552 3708 4044 4440
Size: 3552 156 336 396
Fragments arranged by size:
3552 396 336 156
EarI CTCTTCn'nnn_
Cuts at: 0 1288 1574 2168 2669 3375 3742 4244 4312
Size: 1288 286 594 501 706 367 502 68
Cuts at: 4312 4412 4440
Size: 100 28
Fragments arranged by size:
1288 706 594 502 501 367 286 100
68 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1272 1400 1415 1444 2090 2266 2778
Size: 255 1017 128 15 29 646 176 512
Cuts at: 2778 3155 3429 3809 4440
Size: 377 274 380 631
Fragments arranged by size:
1017 646 631 512 380 377 274 255
176 128 29 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 4440
Size: 706 3734
EcoRI G'AATT_C
Cuts at: 0 2094 3582 4440
Size: 2094 1488 858
Fragments arranged by size:
2094 1488 858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1685 2169 2511 4023 4440
Size: 1685 484 342 1512 417
Fragments arranged by size:
1685 1512 484 417 342
FspI TGC'GCA
Cuts at: 0 1039 4440
Size: 1039 3401
HaeII r_GCGC'y
Cuts at: 0 93 4440
Size: 93 4347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 1635 1667 1906 1938 2232 2264
Size: 130 32 1473 32 239 32 294 32
Cuts at: 2264 3799 3831 4064 4096 4380 4412 4440
Size: 1535 32 233 32 284 32 28
Fragments arranged by size:
1535 1473 294 284 239 233 130 32
32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 1654 2331 2885 3281 3533 4440
Size: 1654 677 554 396 252 907
Fragments arranged by size:
1654 907 677 554 396 252
HindIII A'AGCT_T
Cuts at: 0 3171 4000 4440
Size: 3171 829 440
Fragments arranged by size:
3171 829 440
HpaI GTT'AAC
Cuts at: 0 2331 4440
Size: 2331 2109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 4355 4440
Size: 578 3777 85
Fragments arranged by size:
3777 578 85
MscI TGG'CCA
Cuts at: 0 3554 3710 4046 4440
Size: 3554 156 336 394
Fragments arranged by size:
3554 394 336 156
MslI CAynn'nnrTG
Cuts at: 0 445 2257 2755 3429 3454 3502 4440
Size: 445 1812 498 674 25 48 938
Fragments arranged by size:
1812 938 674 498 445 48 25
MspA1I CmG'CkG
Cuts at: 0 53 4440
Size: 53 4387
NcoI C'CATG_G
Cuts at: 0 4041 4440
Size: 4041 399
NruI TCG'CGA
Cuts at: 0 358 4440
Size: 358 4082
NspI r_CATG'y
Cuts at: 0 1571 3507 4440
Size: 1571 1936 933
Fragments arranged by size:
1936 1571 933
PasI CC'CwG_GG
Cuts at: 0 1064 4440
Size: 1064 3376
PflMI CCAn_nnn'nTGG
Cuts at: 0 3717 4440
Size: 3717 723
PfoI T'CCnGG_A
Cuts at: 0 1483 2032 4440
Size: 1483 549 2408
Fragments arranged by size:
2408 1483 549
PmlI CAC'GTG
Cuts at: 0 3591 4440
Size: 3591 849
PsiI TTA'TAA
Cuts at: 0 482 4440
Size: 482 3958
PvuII CAG'CTG
Cuts at: 0 53 4440
Size: 53 4387
ScaI AGT'ACT
Cuts at: 0 2685 3753 4440
Size: 2685 1068 687
Fragments arranged by size:
2685 1068 687
SfcI C'TryA_G
Cuts at: 0 1166 1906 3011 4440
Size: 1166 740 1105 1429
Fragments arranged by size:
1429 1166 1105 740
SmlI C'TyrA_G
Cuts at: 0 861 2438 4362 4440
Size: 861 1577 1924 78
Fragments arranged by size:
1924 1577 861 78
SphI G_CATG'C
Cuts at: 0 1571 4440
Size: 1571 2869
SspI AAT'ATT
Cuts at: 0 1007 1757 1847 4440
Size: 1007 750 90 2593
Fragments arranged by size:
2593 1007 750 90
StyI C'CwwG_G
Cuts at: 0 2334 2649 4041 4440
Size: 2334 315 1392 399
Fragments arranged by size:
2334 1392 399 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 431 4440
Size: 431 4009
TatI w'GTAC_w
Cuts at: 0 288 737 1071 2683 3421 3751 4440
Size: 288 449 334 1612 738 330 689
Fragments arranged by size:
1612 738 689 449 334 330 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 750 782 3180 3212 4440
Size: 750 32 2398 32 1228
Fragments arranged by size:
2398 1228 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4009 4440
Size: 4009 431
XbaI T'CTAG_A
Cuts at: 0 513 4440
Size: 513 3927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3453 4122 4440
Size: 3453 669 318
Fragments arranged by size:
3453 669 318
XmnI GAAnn'nnTTC
Cuts at: 0 1006 1260 1461 1974 2130 3989 4440
Size: 1006 254 201 513 156 1859 451
Fragments arranged by size:
1859 1006 513 451 254 201 156
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AlwNI ApoI
AseI BaeI BamHI BanI BbsI BcgI BciVI BclI
BglI BglII BlpI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NcoI NruI NspI PasI PflMI PfoI PmlI
PsiI PvuII ScaI SfcI SmlI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BanII BbeI
BbvCI BfrBI Bme1580I BmgBI BmtI BsaBI BsaXI BsiEI
BsiWI BsmBI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII CspCI DrdI EagI EciI EcoICRI EcoO109I EcoRV
FseI FspAI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NsiI PacI PciI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI XhoI XmaI ZraI