Restriction map for
pF1KE2339
(Linear) (Six-base) MAPSORT of: KIEE2339.seq Check: 1135 from: 1 to: 870
KIEE2339.orf
With 182 enzymes: SgfI *
May 6, 2016 19:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 870
Size: 238 632
AgeI A'CCGG_T
Cuts at: 0 580 870
Size: 580 290
AlwNI CAG_nnn'CTG
Cuts at: 0 216 315 870
Size: 216 99 555
Fragments arranged by size:
555 216 99
AvaI C'yCGr_G
Cuts at: 0 84 870
Size: 84 786
BanI G'GyrC_C
Cuts at: 0 523 870
Size: 523 347
BanII G_rGCy'C
Cuts at: 0 206 870
Size: 206 664
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 870
Size: 226 644
BbvCI CC'TCA_GC
Cuts at: 0 43 870
Size: 43 827
BglI GCCn_nnn'nGGC
Cuts at: 0 247 870
Size: 247 623
BglII A'GATC_T
Cuts at: 0 491 870
Size: 491 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 468 870
Size: 366 102 402
Fragments arranged by size:
402 366 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 321 870
Size: 308 13 549
Fragments arranged by size:
549 308 13
Bpu10I CC'TnA_GC
Cuts at: 0 43 870
Size: 43 827
BsaHI Gr'CG_yC
Cuts at: 0 72 605 870
Size: 72 533 265
Fragments arranged by size:
533 265 72
BsaWI w'CCGG_w
Cuts at: 0 580 587 812 870
Size: 580 7 225 58
Fragments arranged by size:
580 225 58 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 870
Size: 319 30 521
Fragments arranged by size:
521 319 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 870
Size: 244 626
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 870
Size: 695 175
BsiEI CG_ry'CG
Cuts at: 0 242 870
Size: 242 628
BsiHKAI G_wGCw'C
Cuts at: 0 206 870
Size: 206 664
BsmI GAATG_Cn'
Cuts at: 0 738 870
Size: 738 132
Bsp1286I G_dGCh'C
Cuts at: 0 206 870
Size: 206 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 607 870
Size: 607 263
BsrFI r'CCGG_y
Cuts at: 0 262 460 580 870
Size: 262 198 120 290
Fragments arranged by size:
290 262 198 120
BstEII G'GTnAC_C
Cuts at: 0 583 870
Size: 583 287
BstYI r'GATC_y
Cuts at: 0 491 590 870
Size: 491 99 280
Fragments arranged by size:
491 280 99
BtgI C'CryG_G
Cuts at: 0 33 137 242 870
Size: 33 104 105 628
Fragments arranged by size:
628 105 104 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 93 200 375 577 727 870
Size: 93 107 175 202 150 143
Fragments arranged by size:
202 175 150 143 107 93
EaeI y'GGCC_r
Cuts at: 0 2 239 716 870
Size: 2 237 477 154
Fragments arranged by size:
477 237 154 2
EagI C'GGCC_G
Cuts at: 0 239 870
Size: 239 631
EcoICRI GAG'CTC
Cuts at: 0 204 870
Size: 204 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 308 321 870
Size: 238 70 13 549
Fragments arranged by size:
549 238 70 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 870
Size: 370 500
EcoO109I rG'GnC_Cy
Cuts at: 0 47 168 213 234 870
Size: 47 121 45 21 636
Fragments arranged by size:
636 121 47 45 21
EcoRV GAT'ATC
Cuts at: 0 297 450 870
Size: 297 153 420
Fragments arranged by size:
420 297 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 637 801 870
Size: 637 164 69
Fragments arranged by size:
637 164 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 319 351 577 609 870
Size: 281 32 6 32 226 32 261
Fragments arranged by size:
281 261 226 32 32 32 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 752 870
Size: 174 578 118
Fragments arranged by size:
578 174 118
MscI TGG'CCA
Cuts at: 0 718 870
Size: 718 152
MspA1I CmG'CkG
Cuts at: 0 35 42 286 870
Size: 35 7 244 584
Fragments arranged by size:
584 244 35 7
PfoI T'CCnGG_A
Cuts at: 0 323 870
Size: 323 547
PpuMI rG'GwC_Cy
Cuts at: 0 234 870
Size: 234 636
PvuII CAG'CTG
Cuts at: 0 286 870
Size: 286 584
SacI G_AGCT'C
Cuts at: 0 206 870
Size: 206 664
SacII CC_GC'GG
Cuts at: 0 36 870
Size: 36 834
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 247 870
Size: 247 623
SgrAI Cr'CCGG_yG
Cuts at: 0 262 580 870
Size: 262 318 290
Fragments arranged by size:
318 290 262
StuI AGG'CCT
Cuts at: 0 277 870
Size: 277 593
StyI C'CwwG_G
Cuts at: 0 164 870
Size: 164 706
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 541 870
Size: 385 156 329
Fragments arranged by size:
385 329 156
TatI w'GTAC_w
Cuts at: 0 101 870
Size: 101 769
XmnI GAAnn'nnTTC
Cuts at: 0 223 870
Size: 223 647
Enzymes that do cut:
AcuI AgeI AlwNI AvaI BanI BanII BbsI BbvCI
BglI BglII BmrI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstEII BstYI BtgI BtgZI EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI Hin4I MmeI MscI MspA1I
PfoI PpuMI PvuII SacI SacII SfiI SgrAI StuI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BseYI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoRI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI