Restriction map for
pF1KE2343
(Linear) (Six-base) MAPSORT of: KIEE2343.seq Check: 5659 from: 1 to: 1959
KIEE2343.orf
With 182 enzymes: SgfI *
May 18, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1521 1959
Size: 1521 438
AatII G_ACGT'C
Cuts at: 0 62 1959
Size: 62 1897
AccI GT'mk_AC
Cuts at: 0 1010 1959
Size: 1010 949
Acc65I G'GTAC_C
Cuts at: 0 494 1959
Size: 494 1465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1959
Size: 814 1145
AfeI AGC'GCT
Cuts at: 0 184 1316 1959
Size: 184 1132 643
Fragments arranged by size:
1132 643 184
AflIII A'CryG_T
Cuts at: 0 842 1959
Size: 842 1117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1333 1959
Size: 1333 626
AlwNI CAG_nnn'CTG
Cuts at: 0 54 1698 1959
Size: 54 1644 261
Fragments arranged by size:
1644 261 54
ApaI G_GGCC'C
Cuts at: 0 384 454 593 821 1056 1122 1160 1894
Size: 384 70 139 228 235 66 38 734
Cuts at: 1894 1959
Size: 65
Fragments arranged by size:
734 384 235 228 139 70 66 65
38
AvaI C'yCGr_G
Cuts at: 0 286 466 696 820 1254 1496 1833 1959
Size: 286 180 230 124 434 242 337 126
Fragments arranged by size:
434 337 286 242 230 180 126 124
AvrII C'CTAG_G
Cuts at: 0 1894 1959
Size: 1894 65
BamHI G'GATC_C
Cuts at: 0 282 1959
Size: 282 1677
BanI G'GyrC_C
Cuts at: 0 271 462 494 501 526 732 772 1348
Size: 271 191 32 7 25 206 40 576
Cuts at: 1348 1393 1533 1597 1959
Size: 45 140 64 362
Fragments arranged by size:
576 362 271 206 191 140 64 45
40 32 25 7
BanII G_rGCy'C
Cuts at: 0 293 384 394 454 593 821 854 1003
Size: 293 91 10 60 139 228 33 149
Cuts at: 1003 1031 1056 1122 1160 1894 1959
Size: 28 25 66 38 734 65
Fragments arranged by size:
734 293 228 149 139 91 66 65
60 38 33 28 25 10
BbeI G_GCGC'C
Cuts at: 0 275 466 505 530 1352 1397 1959
Size: 275 191 39 25 822 45 562
Fragments arranged by size:
822 562 275 191 45 39 25
BbvCI CC'TCA_GC
Cuts at: 0 594 1959
Size: 594 1365
BglI GCCn_nnn'nGGC
Cuts at: 0 868 1370 1432 1959
Size: 868 502 62 527
Fragments arranged by size:
868 527 502 62
Bme1580I G_kGCm'C
Cuts at: 0 141 384 454 593 821 1056 1122 1160
Size: 141 243 70 139 228 235 66 38
Cuts at: 1160 1600 1768 1894 1959
Size: 440 168 126 65
Fragments arranged by size:
440 243 235 228 168 141 139 126
70 66 65 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 391 1959
Size: 391 1568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 216 1959
Size: 216 1743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1144 1231 1402 1888 1959
Size: 1045 99 87 171 486 71
Fragments arranged by size:
1045 486 171 99 87 71
Bpu10I CC'TnA_GC
Cuts at: 0 594 1237 1959
Size: 594 643 722
Fragments arranged by size:
722 643 594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1183 1959
Size: 973 210 776
Fragments arranged by size:
973 776 210
BsaAI yAC'GTr
Cuts at: 0 1662 1959
Size: 1662 297
BsaHI Gr'CG_yC
Cuts at: 0 59 254 272 463 502 527 958 980
Size: 59 195 18 191 39 25 431 22
Cuts at: 980 1349 1394 1959
Size: 369 45 565
Fragments arranged by size:
565 431 369 195 191 59 45 39
25 22 18
BsaWI w'CCGG_w
Cuts at: 0 1951 1959
Size: 1951 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 865 895 943 973 1210 1240
Size: 49 30 786 30 48 30 237 30
Cuts at: 1240 1959
Size: 719
Fragments arranged by size:
786 719 237 49 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 967 1052 1228 1231 1405 1453 1959
Size: 54 913 85 176 3 174 48 506
Fragments arranged by size:
913 506 176 174 85 54 48 3
BseYI C'CCAG_C
Cuts at: 0 23 1320 1344 1715 1791 1959
Size: 23 1297 24 371 76 168
Fragments arranged by size:
1297 371 168 76 24 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1780 1959
Size: 1186 594 179
Fragments arranged by size:
1186 594 179
BsiEI CG_ry'CG
Cuts at: 0 479 554 764 1436 1820 1959
Size: 479 75 210 672 384 139
Fragments arranged by size:
672 479 384 210 139 75
BsiWI C'GTAC_G
Cuts at: 0 845 1959
Size: 845 1114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 66 1959
Size: 66 1893
Bsp1286I G_dGCh'C
Cuts at: 0 141 293 384 394 454 593 821 854
Size: 141 152 91 10 60 139 228 33
Cuts at: 854 1003 1031 1056 1122 1160 1600 1768 1894
Size: 149 28 25 66 38 440 168 126
Cuts at: 1894 1959
Size: 65
Fragments arranged by size:
440 228 168 152 149 141 139 126
91 66 65 60 38 33 28 25
10
BspEI T'CCGG_A
Cuts at: 0 1951 1959
Size: 1951 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1521 1959
Size: 1521 438
BsrBI CCG'CTC
Cuts at: 0 1050 1539 1740 1959
Size: 1050 489 201 219
Fragments arranged by size:
1050 489 219 201
BsrDI GCAATG_nn'
Cuts at: 0 444 1959
Size: 444 1515
BsrFI r'CCGG_y
Cuts at: 0 81 433 478 729 1637 1959
Size: 81 352 45 251 908 322
Fragments arranged by size:
908 352 322 251 81 45
BsrGI T'GTAC_A
Cuts at: 0 605 1959
Size: 605 1354
BssHII G'CGCG_C
Cuts at: 0 515 650 1193 1406 1408 1438 1523 1727
Size: 515 135 543 213 2 30 85 204
Cuts at: 1727 1959
Size: 232
Fragments arranged by size:
543 515 232 213 204 135 85 30
2
BssSI C'ACGA_G
Cuts at: 0 1064 1959
Size: 1064 895
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 1698 1959
Size: 443 1255 261
Fragments arranged by size:
1255 443 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1107 1344 1914 1959
Size: 1107 237 570 45
Fragments arranged by size:
1107 570 237 45
BstYI r'GATC_y
Cuts at: 0 282 1694 1959
Size: 282 1412 265
Fragments arranged by size:
1412 282 265
BtgI C'CryG_G
Cuts at: 0 758 866 893 947 1273 1297 1613 1686
Size: 758 108 27 54 326 24 316 73
Cuts at: 1686 1959
Size: 273
Fragments arranged by size:
758 326 316 273 108 73 54 27
24
BtsI GCAGTG_nn'
Cuts at: 0 161 1174 1959
Size: 161 1013 785
Fragments arranged by size:
1013 785 161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 115 150 954 989 1959
Size: 115 35 804 35 970
Fragments arranged by size:
970 804 115 35 35
EaeI y'GGCC_r
Cuts at: 0 40 295 476 551 761 1433 1817 1880
Size: 40 255 181 75 210 672 384 63
Cuts at: 1880 1959
Size: 79
Fragments arranged by size:
672 384 255 210 181 79 75 63
40
EagI C'GGCC_G
Cuts at: 0 476 551 761 1433 1817 1959
Size: 476 75 210 672 384 142
Fragments arranged by size:
672 476 384 210 142 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1189 1959
Size: 1189 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1045 1144 1231 1402 1888 1959
Size: 814 231 99 87 171 486 71
Fragments arranged by size:
814 486 231 171 99 87 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1417 1959
Size: 1417 542
EcoO109I rG'GnC_Cy
Cuts at: 0 380 381 450 590 817 861 993 1053
Size: 380 1 69 140 227 44 132 60
Cuts at: 1053 1118 1119 1157 1863 1891 1898 1959
Size: 65 1 38 706 28 7 61
Fragments arranged by size:
706 380 227 140 132 69 65 61
60 44 38 28 7 1 1
HaeII r_GCGC'y
Cuts at: 0 186 275 466 505 530 601 1210 1318
Size: 186 89 191 39 25 71 609 108
Cuts at: 1318 1352 1397 1486 1644 1669 1711 1756 1848
Size: 34 45 89 158 25 42 45 92
Cuts at: 1848 1879 1959
Size: 31 80
Fragments arranged by size:
609 191 186 158 108 92 89 89
80 71 45 45 42 39 34 31
25 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 136 168 865 897 943 975
Size: 49 32 55 32 697 32 46 32
Cuts at: 975 1959
Size: 984
Fragments arranged by size:
984 697 55 49 46 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 271 462 501 526 1348 1393 1959
Size: 271 191 39 25 822 45 566
Fragments arranged by size:
822 566 271 191 45 39 25
KpnI G_GTAC'C
Cuts at: 0 498 1959
Size: 498 1461
MluI A'CGCG_T
Cuts at: 0 842 1959
Size: 842 1117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 194 1959
Size: 74 120 1765
Fragments arranged by size:
1765 120 74
MscI TGG'CCA
Cuts at: 0 42 297 1882 1959
Size: 42 255 1585 77
Fragments arranged by size:
1585 255 77 42
MspA1I CmG'CkG
Cuts at: 0 603 895 949 1275 1299 1328 1344 1615
Size: 603 292 54 326 24 29 16 271
Cuts at: 1615 1688 1704 1800 1959
Size: 73 16 96 159
Fragments arranged by size:
603 326 292 271 159 96 73 54
29 24 16 16
NaeI GCC'GGC
Cuts at: 0 83 435 480 731 1639 1959
Size: 83 352 45 251 908 320
Fragments arranged by size:
908 352 320 251 83 45
NarI GG'CG_CC
Cuts at: 0 272 463 502 527 1349 1394 1959
Size: 272 191 39 25 822 45 565
Fragments arranged by size:
822 565 272 191 45 39 25
NgoMIV G'CCGG_C
Cuts at: 0 81 433 478 729 1637 1959
Size: 81 352 45 251 908 322
Fragments arranged by size:
908 352 322 251 81 45
NotI GC'GGCC_GC
Cuts at: 0 1433 1959
Size: 1433 526
PasI CC'CwG_GG
Cuts at: 0 106 237 384 1114 1959
Size: 106 131 147 730 845
Fragments arranged by size:
845 730 147 131 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 1959
Size: 93 1866
PfoI T'CCnGG_A
Cuts at: 0 1043 1959
Size: 1043 916
PpuMI rG'GwC_Cy
Cuts at: 0 993 1863 1959
Size: 993 870 96
Fragments arranged by size:
993 870 96
PspOMI G'GGCC_C
Cuts at: 0 380 450 589 817 1052 1118 1156 1890
Size: 380 70 139 228 235 66 38 734
Cuts at: 1890 1959
Size: 69
Fragments arranged by size:
734 380 235 228 139 70 69 66
38
PstI C_TGCA'G
Cuts at: 0 566 1532 1703 1844 1959
Size: 566 966 171 141 115
Fragments arranged by size:
966 566 171 141 115
PvuII CAG'CTG
Cuts at: 0 1344 1800 1959
Size: 1344 456 159
Fragments arranged by size:
1344 456 159
RsrII CG'GwC_CG
Cuts at: 0 1277 1959
Size: 1277 682
SacII CC_GC'GG
Cuts at: 0 896 950 1276 1300 1616 1689 1959
Size: 896 54 326 24 316 73 270
Fragments arranged by size:
896 326 316 270 73 54 24
ScaI AGT'ACT
Cuts at: 0 199 1959
Size: 199 1760
SfcI C'TryA_G
Cuts at: 0 562 1528 1699 1840 1959
Size: 562 966 171 141 119
Fragments arranged by size:
966 562 171 141 119
SfoI GGC'GCC
Cuts at: 0 273 464 503 528 1350 1395 1959
Size: 273 191 39 25 822 45 564
Fragments arranged by size:
822 564 273 191 45 39 25
SgrAI Cr'CCGG_yG
Cuts at: 0 1637 1959
Size: 1637 322
SmaI CCC'GGG
Cuts at: 0 698 1256 1498 1835 1959
Size: 698 558 242 337 124
Fragments arranged by size:
698 558 337 242 124
SmlI C'TyrA_G
Cuts at: 0 988 1198 1959
Size: 988 210 761
Fragments arranged by size:
988 761 210
StuI AGG'CCT
Cuts at: 0 341 1959
Size: 341 1618
StyI C'CwwG_G
Cuts at: 0 320 857 1883 1894 1907 1959
Size: 320 537 1026 11 13 52
Fragments arranged by size:
1026 537 320 52 13 11
TatI w'GTAC_w
Cuts at: 0 197 605 1959
Size: 197 408 1354
Fragments arranged by size:
1354 408 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1959
Size: 1210 32 717
Fragments arranged by size:
1210 717 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 864 1959
Size: 864 1095
XmaI C'CCGG_G
Cuts at: 0 696 1254 1496 1833 1959
Size: 696 558 242 337 126
Fragments arranged by size:
696 558 337 242 126
ZraI GAC'GTC
Cuts at: 0 60 1959
Size: 60 1899
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AlfI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
CspCI EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI KpnI MluI MmeI MscI MspA1I NaeI
NarI NgoMIV NotI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII RsrII SacII ScaI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TatI TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AleI AloI ApaLI ApoI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BsaI BsaBI BsiHKAI
BsmI BspHI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MslI
NcoI NdeI NheI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacI SalI SanDI SapI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmnI