Restriction map for
pF1KE2344
(Linear) (Six-base) MAPSORT of: KIEE2344.seq Check: 6901 from: 1 to: 4008
KIEE2344.orf
With 182 enzymes: SgfI *
June 23, 2011 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1369 3875 4008
Size: 1369 2506 133
Fragments arranged by size:
2506 1369 133
AatII G_ACGT'C
Cuts at: 0 556 4008
Size: 556 3452
AccI GT'mk_AC
Cuts at: 0 3464 4008
Size: 3464 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1485 1495 2143 4008
Size: 499 986 10 648 1865
Fragments arranged by size:
1865 986 648 499 10
AfeI AGC'GCT
Cuts at: 0 659 1444 1636 2231 4008
Size: 659 785 192 595 1777
Fragments arranged by size:
1777 785 659 595 192
AhdI GACnn_n'nnGTC
Cuts at: 0 318 396 552 4008
Size: 318 78 156 3456
Fragments arranged by size:
3456 318 156 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 2392 4008
Size: 313 2079 1616
Fragments arranged by size:
2079 1616 313
AlwNI CAG_nnn'CTG
Cuts at: 0 999 2430 2687 2742 4008
Size: 999 1431 257 55 1266
Fragments arranged by size:
1431 1266 999 257 55
ApaI G_GGCC'C
Cuts at: 0 37 122 179 211 830 861 1914 2636
Size: 37 85 57 32 619 31 1053 722
Cuts at: 2636 2786 3653 3672 3869 4008
Size: 150 867 19 197 139
Fragments arranged by size:
1053 867 722 619 197 150 139 85
57 37 32 31 19
ApaLI G'TGCA_C
Cuts at: 0 334 430 4008
Size: 334 96 3578
Fragments arranged by size:
3578 334 96
AscI GG'CGCG_CC
Cuts at: 0 3483 3762 4008
Size: 3483 279 246
Fragments arranged by size:
3483 279 246
AvaI C'yCGr_G
Cuts at: 0 29 171 237 746 1732 1949 2779 3196
Size: 29 142 66 509 986 217 830 417
Cuts at: 3196 3322 3433 3472 3814 3994 4008
Size: 126 111 39 342 180 14
Fragments arranged by size:
986 830 509 417 342 217 180 142
126 111 66 39 29 14
AvrII C'CTAG_G
Cuts at: 0 2916 4008
Size: 2916 1092
BanI G'GyrC_C
Cuts at: 0 7 93 439 889 988 1357 1932 2612
Size: 7 86 346 450 99 369 575 680
Cuts at: 2612 2828 2946 2966 3064 3168 3621 3680 3755
Size: 216 118 20 98 104 453 59 75
Cuts at: 3755 3961 4008
Size: 206 47
Fragments arranged by size:
680 575 453 450 369 346 216 206
118 104 99 98 86 75 59 47
20 7
BanII G_rGCy'C
Cuts at: 0 37 122 179 211 475 496 764 830
Size: 37 85 57 32 264 21 268 66
Cuts at: 830 853 861 872 1031 1739 1914 2078 2389
Size: 23 8 11 159 708 175 164 311
Cuts at: 2389 2636 2786 3056 3163 3267 3392 3653 3672
Size: 247 150 270 107 104 125 261 19
Cuts at: 3672 3869 3976 4008
Size: 197 107 32
Fragments arranged by size:
708 311 270 268 264 261 247 197
175 164 159 150 125 107 107 104
85 66 57 37 32 32 23 21
19 11 8
BbeI G_GCGC'C
Cuts at: 0 11 97 443 893 2616 2832 2970 3068
Size: 11 86 346 450 1723 216 138 98
Cuts at: 3068 3172 3625 4008
Size: 104 453 383
Fragments arranged by size:
1723 453 450 383 346 216 138 104
98 86 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 359 2340 2535 2577 4008
Size: 339 20 1981 195 42 1431
Fragments arranged by size:
1981 1431 339 195 42 20
BbvCI CC'TCA_GC
Cuts at: 0 779 3823 4008
Size: 779 3044 185
Fragments arranged by size:
3044 779 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 905 939 2331 2365 4008
Size: 905 34 1392 34 1643
Fragments arranged by size:
1643 1392 905 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2055 4008
Size: 2055 1953
BglI GCCn_nnn'nGGC
Cuts at: 0 23 32 196 220 906 979 2029 3109
Size: 23 9 164 24 686 73 1050 1080
Cuts at: 3109 3365 3608 4008
Size: 256 243 400
Fragments arranged by size:
1080 1050 686 400 256 243 164 73
24 23 9
BlpI GC'TnA_GC
Cuts at: 0 2990 4008
Size: 2990 1018
Bme1580I G_kGCm'C
Cuts at: 0 37 122 179 211 338 434 582 702
Size: 37 85 57 32 127 96 148 120
Cuts at: 702 830 861 993 1914 1937 2141 2636 2786
Size: 128 31 132 921 23 204 495 150
Cuts at: 2786 2801 3244 3310 3653 3672 3685 3835 3869
Size: 15 443 66 343 19 13 150 34
Cuts at: 3869 3964 4008
Size: 95 44
Fragments arranged by size:
921 495 443 343 204 150 150 148
132 128 127 120 96 95 85 66
57 44 37 34 32 31 23 19
15 13
BmgBI CAC'GTC
Cuts at: 0 249 1190 4008
Size: 249 941 2818
Fragments arranged by size:
2818 941 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 3915 4008
Size: 3915 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 1653 2829 4008
Size: 489 1164 1176 1179
Fragments arranged by size:
1179 1176 1164 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 860 1330 2476 2652 3406 3577 4008
Size: 556 304 470 1146 176 754 171 431
Fragments arranged by size:
1146 754 556 470 431 304 176 171
Bpu10I CC'TnA_GC
Cuts at: 0 779 1025 3823 4008
Size: 779 246 2798 185
Fragments arranged by size:
2798 779 246 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 2227 4008
Size: 1216 1011 1781
Fragments arranged by size:
1781 1216 1011
BsaI GGTCTCn'nnnn_
Cuts at: 0 1666 4008
Size: 1666 2342
BsaAI yAC'GTr
Cuts at: 0 1103 2058 4008
Size: 1103 955 1950
Fragments arranged by size:
1950 1103 955
BsaHI Gr'CG_yC
Cuts at: 0 8 94 440 553 890 1556 2261 2540
Size: 8 86 346 113 337 666 705 279
Cuts at: 2540 2613 2829 2967 3065 3169 3622 3663 4008
Size: 73 216 138 98 104 453 41 345
Fragments arranged by size:
705 666 453 346 345 337 279 216
138 113 104 98 86 73 41 8
BsaWI w'CCGG_w
Cuts at: 0 1419 3935 4008
Size: 1419 2516 73
Fragments arranged by size:
2516 1419 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2371 2401 2449 2479 3379 3409 4008
Size: 2371 30 48 30 900 30 599
Fragments arranged by size:
2371 900 599 48 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 553 766 796 1645 2065 3275 4008
Size: 103 450 213 30 849 420 1210 733
Fragments arranged by size:
1210 849 733 450 420 213 103 30
BseYI C'CCAG_C
Cuts at: 0 489 641 701 830 1208 2746 3213 3375
Size: 489 152 60 129 378 1538 467 162
Cuts at: 3375 3494 3769 3776 4008
Size: 119 275 7 232
Fragments arranged by size:
1538 489 467 378 275 232 162 152
129 119 60 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2362 4008
Size: 2362 1646
BsiEI CG_ry'CG
Cuts at: 0 148 191 215 2773 2987 3272 3450 3464
Size: 148 43 24 2558 214 285 178 14
Cuts at: 3464 3527 3691 4008
Size: 63 164 317
Fragments arranged by size:
2558 317 285 214 178 164 148 63
43 24 14
BsiHKAI G_wGCw'C
Cuts at: 0 272 338 434 455 650 695 1031 2288
Size: 272 66 96 21 195 45 336 1257
Cuts at: 2288 2600 4008
Size: 312 1408
Fragments arranged by size:
1408 1257 336 312 272 195 96 66
45 21
BsiWI C'GTAC_G
Cuts at: 0 182 3030 4008
Size: 182 2848 978
Fragments arranged by size:
2848 978 182
Bsp1286I G_dGCh'C
Cuts at: 0 37 122 179 211 272 338 434 455
Size: 37 85 57 32 61 66 96 21
Cuts at: 455 475 496 582 650 695 702 764 830
Size: 20 21 86 68 45 7 62 66
Cuts at: 830 853 861 872 993 1031 1739 1914 1937
Size: 23 8 11 121 38 708 175 23
Cuts at: 1937 2078 2141 2288 2389 2600 2636 2786 2801
Size: 141 63 147 101 211 36 150 15
Cuts at: 2801 3056 3163 3244 3267 3310 3392 3653 3672
Size: 255 107 81 23 43 82 261 19
Cuts at: 3672 3685 3835 3869 3964 3976 4008
Size: 13 150 34 95 12 32
Fragments arranged by size:
708 261 255 211 175 150 150 147
141 121 107 101 96 95 86 85
82 81 68 66 66 63 62 61
57 45 43 38 37 36 34 32
32 23 23 23 21 21 20 19
15 13 12 11 8 7
BspHI T'CATG_A
Cuts at: 0 1091 2039 4008
Size: 1091 948 1969
Fragments arranged by size:
1969 1091 948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 1369 3512 3875 4008
Size: 323 1046 2143 363 133
Fragments arranged by size:
2143 1046 363 323 133
BsrBI CCG'CTC
Cuts at: 0 150 2460 2989 3274 4008
Size: 150 2310 529 285 734
Fragments arranged by size:
2310 734 529 285 150
BsrDI GCAATG_nn'
Cuts at: 0 1710 4008
Size: 1710 2298
BsrFI r'CCGG_y
Cuts at: 0 10 112 1675 2772 2982 3061 3165 3758
Size: 10 102 1563 1097 210 79 104 593
Cuts at: 3758 4008
Size: 250
Fragments arranged by size:
1563 1097 593 250 210 104 102 79
10
BsrGI T'GTAC_A
Cuts at: 0 2678 4008
Size: 2678 1330
BssHII G'CGCG_C
Cuts at: 0 363 365 719 1593 1696 2808 2938 2940
Size: 363 2 354 874 103 1112 130 2
Cuts at: 2940 3483 3762 3850 4008
Size: 543 279 88 158
Fragments arranged by size:
1112 874 543 363 354 279 158 130
103 88 2 2
BssSI C'ACGA_G
Cuts at: 0 163 295 448 575 3828 4008
Size: 163 132 153 127 3253 180
Fragments arranged by size:
3253 180 163 153 132 127
BstBI TT'CG_AA
Cuts at: 0 350 4008
Size: 350 3658
BstEII G'GTnAC_C
Cuts at: 0 1521 4008
Size: 1521 2487
BstXI CCAn_nnnn'nTGG
Cuts at: 0 838 2412 4008
Size: 838 1574 1596
Fragments arranged by size:
1596 1574 838
BstYI r'GATC_y
Cuts at: 0 725 799 2477 4008
Size: 725 74 1678 1531
Fragments arranged by size:
1678 1531 725 74
BtgI C'CryG_G
Cuts at: 0 21 215 257 651 1065 1850 1937 2150
Size: 21 194 42 394 414 785 87 213
Cuts at: 2150 2564 2767 2950 3107 3685 3706 4008
Size: 414 203 183 157 578 21 302
Fragments arranged by size:
785 578 414 414 394 302 213 203
194 183 157 87 42 21 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2355 4008
Size: 2355 1653
BtsI GCAGTG_nn'
Cuts at: 0 717 923 2288 2622 3543 4008
Size: 717 206 1365 334 921 465
Fragments arranged by size:
1365 921 717 465 334 206
ClaI AT'CG_AT
Cuts at: 0 1550 4008
Size: 1550 2458
DraIII CAC_nnn'GTG
Cuts at: 0 342 2604 4008
Size: 342 2262 1404
Fragments arranged by size:
2262 1404 342
DrdI GACnn_nn'nnGTC
Cuts at: 0 1657 4008
Size: 1657 2351
EaeI y'GGCC_r
Cuts at: 0 145 188 212 425 585 971 980 1032
Size: 145 43 24 213 160 386 9 52
Cuts at: 1032 1301 1604 1847 2030 2561 2770 2870 2984
Size: 269 303 243 183 531 209 100 114
Cuts at: 2984 3110 3181 3269 3447 3524 4008
Size: 126 71 88 178 77 484
Fragments arranged by size:
531 484 386 303 269 243 213 209
183 178 160 145 126 114 100 88
77 71 52 43 24 9
EagI C'GGCC_G
Cuts at: 0 145 188 212 2770 2984 3269 3447 3524
Size: 145 43 24 2558 214 285 178 77
Cuts at: 3524 4008
Size: 484
Fragments arranged by size:
2558 484 285 214 178 145 77 43
24
EarI CTCTTCn'nnn_
Cuts at: 0 501 742 881 4008
Size: 501 241 139 3127
Fragments arranged by size:
3127 501 241 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 1216 3266 3272 4008
Size: 718 498 2050 6 736
Fragments arranged by size:
2050 736 718 498 6
EcoICRI GAG'CTC
Cuts at: 0 1029 4008
Size: 1029 2979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 556 860 1330 1485 1495 2143 2476
Size: 499 57 304 470 155 10 648 333
Cuts at: 2476 2652 3406 3577 4008
Size: 176 754 171 431
Fragments arranged by size:
754 648 499 470 431 333 304 176
171 155 57 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2380 2667 4008
Size: 2380 287 1341
Fragments arranged by size:
2380 1341 287
EcoO109I rG'GnC_Cy
Cuts at: 0 33 34 175 826 827 857 858 996
Size: 33 1 141 651 1 30 1 138
Cuts at: 996 1911 2099 2632 2913 3650 3780 3819 3866
Size: 915 188 533 281 737 130 39 47
Cuts at: 3866 3954 4008
Size: 88 54
Fragments arranged by size:
915 737 651 533 281 188 141 138
130 88 54 47 39 33 30 1
1 1
FspI TGC'GCA
Cuts at: 0 232 4008
Size: 232 3776
HaeII r_GCGC'y
Cuts at: 0 11 64 97 163 265 443 661 893
Size: 11 53 33 66 102 178 218 232
Cuts at: 893 1446 1638 1863 2233 2616 2696 2832 2970
Size: 553 192 225 370 383 80 136 138
Cuts at: 2970 3068 3172 3432 3625 4008
Size: 98 104 260 193 383
Fragments arranged by size:
553 383 383 370 260 232 225 218
193 192 178 138 136 104 102 98
80 66 53 33 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 382 414 538 570 1361 1393
Size: 304 32 46 32 124 32 791 32
Cuts at: 1393 2053 2085 3811 3843 4008
Size: 660 32 1726 32 165
Fragments arranged by size:
1726 791 660 304 165 124 46 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3465 4008
Size: 3465 543
HindIII A'AGCT_T
Cuts at: 0 2320 4008
Size: 2320 1688
KasI G'GCGC_C
Cuts at: 0 7 93 439 889 2612 2828 2966 3064
Size: 7 86 346 450 1723 216 138 98
Cuts at: 3064 3168 3621 4008
Size: 104 453 387
Fragments arranged by size:
1723 453 450 387 346 216 138 104
98 86 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3317 4008
Size: 3317 691
MscI TGG'CCA
Cuts at: 0 587 982 1303 1606 1849 2563 4008
Size: 587 395 321 303 243 714 1445
Fragments arranged by size:
1445 714 587 395 321 303 243
MslI CAynn'nnrTG
Cuts at: 0 613 4008
Size: 613 3395
MspA1I CmG'CkG
Cuts at: 0 23 489 653 778 834 964 1067 1208
Size: 23 466 164 125 56 130 103 141
Cuts at: 1208 1242 1355 2393 2684 2769 2825 2952 2973
Size: 34 113 1038 291 85 56 127 21
Cuts at: 2973 3109 3518 3587 3674 3687 3708 3769 3918
Size: 136 409 69 87 13 21 61 149
Cuts at: 3918 4008
Size: 90
Fragments arranged by size:
1038 466 409 291 164 149 141 136
130 127 125 113 103 90 87 85
69 61 56 56 34 23 21 21
13
NaeI GCC'GGC
Cuts at: 0 2774 2984 3063 3167 4008
Size: 2774 210 79 104 841
Fragments arranged by size:
2774 841 210 104 79
NarI GG'CG_CC
Cuts at: 0 8 94 440 890 2613 2829 2967 3065
Size: 8 86 346 450 1723 216 138 98
Cuts at: 3065 3169 3622 4008
Size: 104 453 386
Fragments arranged by size:
1723 453 450 386 346 216 138 104
98 86 8
NcoI C'CATG_G
Cuts at: 0 2564 4008
Size: 2564 1444
NgoMIV G'CCGG_C
Cuts at: 0 2772 2982 3061 3165 4008
Size: 2772 210 79 104 843
Fragments arranged by size:
2772 843 210 104 79
NotI GC'GGCC_GC
Cuts at: 0 145 4008
Size: 145 3863
NspI r_CATG'y
Cuts at: 0 2187 2553 2679 4008
Size: 2187 366 126 1329
Fragments arranged by size:
2187 1329 366 126
PasI CC'CwG_GG
Cuts at: 0 673 3638 4008
Size: 673 2965 370
Fragments arranged by size:
2965 673 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 2646 4008
Size: 2646 1362
PfoI T'CCnGG_A
Cuts at: 0 83 2045 4008
Size: 83 1962 1963
Fragments arranged by size:
1963 1962 83
PmlI CAC'GTG
Cuts at: 0 1103 4008
Size: 1103 2905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 4008
Size: 751 32 3225
Fragments arranged by size:
3225 751 32
PpuMI rG'GwC_Cy
Cuts at: 0 3780 3819 3954 4008
Size: 3780 39 135 54
Fragments arranged by size:
3780 135 54 39
PshAI GACnn'nnGTC
Cuts at: 0 629 4008
Size: 629 3379
PspOMI G'GGCC_C
Cuts at: 0 33 118 175 207 826 857 1910 2632
Size: 33 85 57 32 619 31 1053 722
Cuts at: 2632 2782 3649 3668 3865 4008
Size: 150 867 19 197 143
Fragments arranged by size:
1053 867 722 619 197 150 143 85
57 33 32 31 19
PspXI vC'TCGA_Gb
Cuts at: 0 1732 3814 3994 4008
Size: 1732 2082 180 14
Fragments arranged by size:
2082 1732 180 14
PstI C_TGCA'G
Cuts at: 0 305 488 1271 1344 2283 2426 2692 4008
Size: 305 183 783 73 939 143 266 1316
Fragments arranged by size:
1316 939 783 305 266 183 143 73
PvuII CAG'CTG
Cuts at: 0 489 778 834 964 1208 2684 3587 4008
Size: 489 289 56 130 244 1476 903 421
Fragments arranged by size:
1476 903 489 421 289 244 130 56
RsrII CG'GwC_CG
Cuts at: 0 3520 4008
Size: 3520 488
SacI G_AGCT'C
Cuts at: 0 1031 4008
Size: 1031 2977
SacII CC_GC'GG
Cuts at: 0 24 654 1068 2770 2953 3110 3688 3709
Size: 24 630 414 1702 183 157 578 21
Cuts at: 3709 4008
Size: 299
Fragments arranged by size:
1702 630 578 414 299 183 157 24
21
SalI G'TCGA_C
Cuts at: 0 3463 4008
Size: 3463 545
SapI GCTCTTCn'nnn_
Cuts at: 0 501 742 4008
Size: 501 241 3266
Fragments arranged by size:
3266 501 241
SexAI A'CCwGG_T
Cuts at: 0 1517 2600 4008
Size: 1517 1083 1408
Fragments arranged by size:
1517 1408 1083
SfcI C'TryA_G
Cuts at: 0 301 484 1267 1340 1740 2279 2422 2688
Size: 301 183 783 73 400 539 143 266
Cuts at: 2688 4008
Size: 1320
Fragments arranged by size:
1320 783 539 400 301 266 183 143
73
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 23 32 979 4008
Size: 23 9 947 3029
Fragments arranged by size:
3029 947 23 9
SfoI GGC'GCC
Cuts at: 0 9 95 441 891 2614 2830 2968 3066
Size: 9 86 346 450 1723 216 138 98
Cuts at: 3066 3170 3623 4008
Size: 104 453 385
Fragments arranged by size:
1723 453 450 385 346 216 138 104
98 86 9
SgrAI Cr'CCGG_yG
Cuts at: 0 10 3061 3165 3758 4008
Size: 10 3051 104 593 250
Fragments arranged by size:
3051 593 250 104 10
SmaI CCC'GGG
Cuts at: 0 239 1951 2781 4008
Size: 239 1712 830 1227
Fragments arranged by size:
1712 1227 830 239
SmlI C'TyrA_G
Cuts at: 0 1231 1732 2242 3322 3814 3994 4008
Size: 1231 501 510 1080 492 180 14
Fragments arranged by size:
1231 1080 510 501 492 180 14
SrfI GCCC'GGGC
Cuts at: 0 239 4008
Size: 239 3769
StuI AGG'CCT
Cuts at: 0 2921 4008
Size: 2921 1087
StyI C'CwwG_G
Cuts at: 0 461 2564 2837 2916 4008
Size: 461 2103 273 79 1092
Fragments arranged by size:
2103 1092 461 273 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1075 1746 2653 3450 4008
Size: 1075 671 907 797 558
Fragments arranged by size:
1075 907 797 671 558
TatI w'GTAC_w
Cuts at: 0 2606 2678 4008
Size: 2606 72 1330
Fragments arranged by size:
2606 1330 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2371 2403 2449 2481 3379 3411 4008
Size: 2371 32 46 32 898 32 597
Fragments arranged by size:
2371 898 597 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1375 4008
Size: 1375 2633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1968 2643 4008
Size: 1968 675 1365
Fragments arranged by size:
1968 1365 675
XhoI C'TCGA_G
Cuts at: 0 1732 3322 3814 3994 4008
Size: 1732 1590 492 180 14
Fragments arranged by size:
1732 1590 492 180 14
XmaI C'CCGG_G
Cuts at: 0 237 1949 2779 4008
Size: 237 1712 830 1229
Fragments arranged by size:
1712 1229 830 237
ZraI GAC'GTC
Cuts at: 0 554 4008
Size: 554 3454
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AlfI AlwNI
ApaI ApaLI AscI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII RsrII SacI
SacII SalI SapI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AleI AloI ApoI
AseI AsiSI BaeI BamHI BclI BfrBI BglII BmtI
BsaBI BsmI BsmBI BspEI BstAPI BstZ17I Bsu36I CspCI
DraI EcoRI EcoRV FalI FseI FspAI HpaI KpnI
MfeI MluI NdeI NheI NruI NsiI PacI PciI
PmeI PsiI PsrI PvuI SanDI SbfI ScaI SgfI
SnaBI SpeI SphI SspI SwaI XbaI XmnI