Restriction map for
pF1KE2347
(Linear) (Six-base) MAPSORT of: KIEE2347.seq Check: 8012 from: 1 to: 2214
KIEE2347.orf
With 182 enzymes: SgfI *
June 23, 2011 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 290 2214
Size: 290 1924
AccI GT'mk_AC
Cuts at: 0 698 2214
Size: 698 1516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1841 2214
Size: 698 1143 373
Fragments arranged by size:
1143 698 373
AhdI GACnn_n'nnGTC
Cuts at: 0 1718 2214
Size: 1718 496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 794 2214
Size: 794 1420
AlwNI CAG_nnn'CTG
Cuts at: 0 373 835 859 1561 2214
Size: 373 462 24 702 653
Fragments arranged by size:
702 653 462 373 24
ApaI G_GGCC'C
Cuts at: 0 167 894 2214
Size: 167 727 1320
Fragments arranged by size:
1320 727 167
ApoI r'AATT_y
Cuts at: 0 1851 1958 2214
Size: 1851 107 256
Fragments arranged by size:
1851 256 107
AvaI C'yCGr_G
Cuts at: 0 34 109 354 421 1181 2214
Size: 34 75 245 67 760 1033
Fragments arranged by size:
1033 760 245 75 67 34
AvrII C'CTAG_G
Cuts at: 0 645 2214
Size: 645 1569
BanI G'GyrC_C
Cuts at: 0 232 414 963 1388 1518 2214
Size: 232 182 549 425 130 696
Fragments arranged by size:
696 549 425 232 182 130
BanII G_rGCy'C
Cuts at: 0 49 167 282 653 881 894 1211 1348
Size: 49 118 115 371 228 13 317 137
Cuts at: 1348 1403 1418 2214
Size: 55 15 796
Fragments arranged by size:
796 371 317 228 137 118 115 55
49 15 13
BbeI G_GCGC'C
Cuts at: 0 236 418 1392 2214
Size: 236 182 974 822
Fragments arranged by size:
974 822 236 182
BbvCI CC'TCA_GC
Cuts at: 0 1061 1621 1799 2214
Size: 1061 560 178 415
Fragments arranged by size:
1061 560 415 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1111 1145 2214
Size: 1111 34 1069
Fragments arranged by size:
1111 1069 34
BglI GCCn_nnn'nGGC
Cuts at: 0 27 63 147 2214
Size: 27 36 84 2067
Fragments arranged by size:
2067 84 36 27
Bme1580I G_kGCm'C
Cuts at: 0 167 894 1272 1523 2214
Size: 167 727 378 251 691
Fragments arranged by size:
727 691 378 251 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 1214 1384 1681 2214
Size: 1214 170 297 533
Fragments arranged by size:
1214 533 297 170
BmtI G_CTAG'C
Cuts at: 0 1060 2214
Size: 1060 1154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 953 1426 2214
Size: 222 731 473 788
Fragments arranged by size:
788 731 473 222
Bpu10I CC'TnA_GC
Cuts at: 0 497 880 1061 1621 1799 2214
Size: 497 383 181 560 178 415
Fragments arranged by size:
560 497 415 383 181 178
BsaHI Gr'CG_yC
Cuts at: 0 233 245 415 1389 2214
Size: 233 12 170 974 825
Fragments arranged by size:
974 825 233 170 12
BsaWI w'CCGG_w
Cuts at: 0 1213 1847 2214
Size: 1213 634 367
Fragments arranged by size:
1213 634 367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 320 747 1888 2214
Size: 219 101 427 1141 326
Fragments arranged by size:
1141 427 326 219 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1400 2214
Size: 821 579 814
Fragments arranged by size:
821 814 579
BsiEI CG_ry'CG
Cuts at: 0 455 2214
Size: 455 1759
BsiHKAI G_wGCw'C
Cuts at: 0 1348 1403 1941 2214
Size: 1348 55 538 273
Fragments arranged by size:
1348 538 273 55
BsmI GAATG_Cn'
Cuts at: 0 1174 2094 2214
Size: 1174 920 120
Fragments arranged by size:
1174 920 120
Bsp1286I G_dGCh'C
Cuts at: 0 49 167 282 653 881 894 1211 1272
Size: 49 118 115 371 228 13 317 61
Cuts at: 1272 1348 1403 1418 1523 1941 2214
Size: 76 55 15 105 418 273
Fragments arranged by size:
418 371 317 273 228 118 115 105
76 61 55 49 15 13
BspEI T'CCGG_A
Cuts at: 0 1213 1847 2214
Size: 1213 634 367
Fragments arranged by size:
1213 634 367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 2214
Size: 290 1924
BsrBI CCG'CTC
Cuts at: 0 209 2214
Size: 209 2005
BsrDI GCAATG_nn'
Cuts at: 0 980 1760 2214
Size: 980 780 454
Fragments arranged by size:
980 780 454
BsrFI r'CCGG_y
Cuts at: 0 72 141 408 2214
Size: 72 69 267 1806
Fragments arranged by size:
1806 267 72 69
BsrGI T'GTAC_A
Cuts at: 0 1241 2214
Size: 1241 973
BssHII G'CGCG_C
Cuts at: 0 193 342 2214
Size: 193 149 1872
Fragments arranged by size:
1872 193 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 1984 2214
Size: 373 1611 230
Fragments arranged by size:
1611 373 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1140 1836 1989 2214
Size: 1140 696 153 225
Fragments arranged by size:
1140 696 225 153
BstYI r'GATC_y
Cuts at: 0 1558 1699 2214
Size: 1558 141 515
Fragments arranged by size:
1558 515 141
Bsu36I CC'TnA_GG
Cuts at: 0 136 2214
Size: 136 2078
BtgI C'CryG_G
Cuts at: 0 92 1280 2214
Size: 92 1188 934
Fragments arranged by size:
1188 934 92
BtsI GCAGTG_nn'
Cuts at: 0 833 1272 1841 2214
Size: 833 439 569 373
Fragments arranged by size:
833 569 439 373
EaeI y'GGCC_r
Cuts at: 0 19 95 452 941 1662 1826 2214
Size: 19 76 357 489 721 164 388
Fragments arranged by size:
721 489 388 357 164 76 19
EagI C'GGCC_G
Cuts at: 0 452 2214
Size: 452 1762
EarI CTCTTCn'nnn_
Cuts at: 0 1299 2141 2214
Size: 1299 842 73
Fragments arranged by size:
1299 842 73
EcoICRI GAG'CTC
Cuts at: 0 1346 1401 2214
Size: 1346 55 813
Fragments arranged by size:
1346 813 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 698 953 1426 1841 2214
Size: 222 476 255 473 415 373
Fragments arranged by size:
476 473 415 373 255 222
EcoO109I rG'GnC_Cy
Cuts at: 0 7 113 163 890 2214
Size: 7 106 50 727 1324
Fragments arranged by size:
1324 727 106 50 7
EcoRV GAT'ATC
Cuts at: 0 1003 2214
Size: 1003 1211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2170 2214
Size: 2170 44
HaeII r_GCGC'y
Cuts at: 0 236 329 418 504 852 1392 2214
Size: 236 93 89 86 348 540 822
Fragments arranged by size:
822 540 348 236 93 89 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1704 1736 1771 1803 2214
Size: 1704 32 35 32 411
Fragments arranged by size:
1704 411 35 32 32
HindIII A'AGCT_T
Cuts at: 0 1458 2214
Size: 1458 756
KasI G'GCGC_C
Cuts at: 0 232 414 1388 2214
Size: 232 182 974 826
Fragments arranged by size:
974 826 232 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 504 1302 1860 2214
Size: 165 339 798 558 354
Fragments arranged by size:
798 558 354 339 165
MscI TGG'CCA
Cuts at: 0 943 1664 1828 2214
Size: 943 721 164 386
Fragments arranged by size:
943 721 386 164
MslI CAynn'nnrTG
Cuts at: 0 913 1279 2214
Size: 913 366 935
Fragments arranged by size:
935 913 366
MspA1I CmG'CkG
Cuts at: 0 24 94 200 296 373 451 517 1798
Size: 24 70 106 96 77 78 66 1281
Cuts at: 1798 1920 2214
Size: 122 294
Fragments arranged by size:
1281 294 122 106 96 78 77 70
66 24
NaeI GCC'GGC
Cuts at: 0 143 410 2214
Size: 143 267 1804
Fragments arranged by size:
1804 267 143
NarI GG'CG_CC
Cuts at: 0 233 415 1389 2214
Size: 233 182 974 825
Fragments arranged by size:
974 825 233 182
NgoMIV G'CCGG_C
Cuts at: 0 141 408 2214
Size: 141 267 1806
Fragments arranged by size:
1806 267 141
NheI G'CTAG_C
Cuts at: 0 1056 2214
Size: 1056 1158
PflMI CCAn_nnn'nTGG
Cuts at: 0 1815 2214
Size: 1815 399
PfoI T'CCnGG_A
Cuts at: 0 461 1403 2214
Size: 461 942 811
Fragments arranged by size:
942 811 461
PpuMI rG'GwC_Cy
Cuts at: 0 113 2214
Size: 113 2101
PshAI GACnn'nnGTC
Cuts at: 0 1021 1673 2214
Size: 1021 652 541
Fragments arranged by size:
1021 652 541
PspOMI G'GGCC_C
Cuts at: 0 163 890 2214
Size: 163 727 1324
Fragments arranged by size:
1324 727 163
PspXI vC'TCGA_Gb
Cuts at: 0 34 2214
Size: 34 2180
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1073 1105 2214
Size: 1073 32 1109
Fragments arranged by size:
1109 1073 32
PstI C_TGCA'G
Cuts at: 0 301 2214
Size: 301 1913
PvuII CAG'CTG
Cuts at: 0 296 517 2214
Size: 296 221 1697
Fragments arranged by size:
1697 296 221
SacI G_AGCT'C
Cuts at: 0 1348 1403 2214
Size: 1348 55 811
Fragments arranged by size:
1348 811 55
SacII CC_GC'GG
Cuts at: 0 95 2214
Size: 95 2119
SanDI GG'GwC_CC
Cuts at: 0 113 2214
Size: 113 2101
SapI GCTCTTCn'nnn_
Cuts at: 0 2141 2214
Size: 2141 73
SfcI C'TryA_G
Cuts at: 0 297 618 2214
Size: 297 321 1596
Fragments arranged by size:
1596 321 297
SfoI GGC'GCC
Cuts at: 0 234 416 1390 2214
Size: 234 182 974 824
Fragments arranged by size:
974 824 234 182
SmaI CCC'GGG
Cuts at: 0 111 356 423 1183 2214
Size: 111 245 67 760 1031
Fragments arranged by size:
1031 760 245 111 67
SmlI C'TyrA_G
Cuts at: 0 34 2214
Size: 34 2180
SrfI GCCC'GGGC
Cuts at: 0 1183 2214
Size: 1183 1031
StuI AGG'CCT
Cuts at: 0 394 2214
Size: 394 1820
StyI C'CwwG_G
Cuts at: 0 645 1860 2115 2214
Size: 645 1215 255 99
Fragments arranged by size:
1215 645 255 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1273 1366 2214
Size: 1273 93 848
Fragments arranged by size:
1273 848 93
TatI w'GTAC_w
Cuts at: 0 1241 2214
Size: 1241 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2036 2068 2214
Size: 2036 32 146
Fragments arranged by size:
2036 146 32
XbaI T'CTAG_A
Cuts at: 0 2025 2214
Size: 2025 189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 2214
Size: 609 1605
XhoI C'TCGA_G
Cuts at: 0 34 2214
Size: 34 2180
XmaI C'CCGG_G
Cuts at: 0 109 354 421 1181 2214
Size: 109 245 67 760 1033
Fragments arranged by size:
1033 760 245 109 67
XmnI GAAnn'nnTTC
Cuts at: 0 1459 2214
Size: 1459 755
Enzymes that do cut:
AarI AccI AcuI AhdI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI
Bme1580I BmrI BmtI BpmI Bpu10I BsaHI BsaWI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SacII SanDI SapI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbsI
BciVI BclI BfrBI BglII BlpI BmgBI BplI BpuEI
BsaI BsaAI BsaBI BsaXI BseYI BsiWI BsmBI BspHI
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoRI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI NcoI NdeI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PsiI PvuI RsrII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I ZraI