Restriction map for
pF1KE2356
(Linear) (Six-base) MAPSORT of: KIEE2356.seq Check: 1760 from: 1 to: 8175
KIEE2356.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4422 5746 8175
Size: 4422 1324 2429
Fragments arranged by size:
4422 2429 1324
AatII G_ACGT'C
Cuts at: 0 4256 8175
Size: 4256 3919
AccI GT'mk_AC
Cuts at: 0 935 8175
Size: 935 7240
Acc65I G'GTAC_C
Cuts at: 0 5098 8175
Size: 5098 3077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 971 2125 5255 5920 5998 8175
Size: 400 571 1154 3130 665 78 2177
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 296 5891 8175
Size: 296 5595 2284
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 7558 8175
Size: 7558 617
AleI CACnn'nnGTG
Cuts at: 0 1798 2641 3907 4456 5890 8175
Size: 1798 843 1266 549 1434 2285
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 5803 8175
Size: 889 4914 2372
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1579 1611 8175
Size: 1579 32 6564
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 492 2443 3772 4525 6458 7010 8175
Size: 492 1951 1329 753 1933 552 1165
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 1832 2434 2623 3104 3304 4377 4450 4535
Size: 1832 602 189 481 200 1073 73 85
Cuts at: 4535 4966 5695 6854 7927 8175
Size: 431 729 1159 1073 248
Fragments arranged by size:
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248 200 189 85 73
ApaLI G'TGCA_C
Cuts at: 0 1918 7330 8014 8175
Size: 1918 5412 684 161
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 3709 8175
Size: 3709 4466
AvaI C'yCGr_G
Cuts at: 0 220 310 319 653 2290 3207 3462 3885
Size: 220 90 9 334 1637 917 255 423
Cuts at: 3885 4376 4741 5125 5415 5811 5952 7016 7307
Size: 491 365 384 290 396 141 1064 291
Cuts at: 7307 7325 7341 7543 7602 7919 8175
Size: 18 16 202 59 317 256
Fragments arranged by size:
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141 90 59 18 16 9
BamHI G'GATC_C
Cuts at: 0 503 6694 8175
Size: 503 6191 1481
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 516 574 690 793 1366 1507 1620 1792
Size: 516 58 116 103 573 141 113 172
Cuts at: 1792 2170 2880 3138 3232 3628 3664 3928 4135
Size: 378 710 258 94 396 36 264 207
Cuts at: 4135 4422 4495 4569 4951 5098 5278 5316 5458
Size: 287 73 74 382 147 180 38 142
Cuts at: 5458 5734 5878 6316 6429 6937 7194 7303 7450
Size: 276 144 438 113 508 257 109 147
Cuts at: 7450 7990 8175
Size: 540 185
Fragments arranged by size:
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378 287 276 264 258 257 207 185
180 172 147 147 144 142 141 116
113 113 109 103 94 74 73 58
38 36
BanII G_rGCy'C
Cuts at: 0 203 1526 1699 1832 2257 2351 2434 2623
Size: 203 1323 173 133 425 94 83 189
Cuts at: 2623 2863 3104 3304 4015 4294 4377 4450 4535
Size: 240 241 200 711 279 83 73 85
Cuts at: 4535 4966 5069 5231 5393 5545 5695 5821 5969
Size: 431 103 162 162 152 150 126 148
Cuts at: 5969 6314 6854 7262 7544 7867 7927 8175
Size: 345 540 408 282 323 60 248
Fragments arranged by size:
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282 279 248 241 240 203 200 189
173 162 162 152 150 148 133 126
103 94 85 83 83 73 60
BbeI G_GCGC'C
Cuts at: 0 578 4426 5282 8175
Size: 578 3848 856 2893
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 2332 5428 8175
Size: 2332 3096 2747
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 190 2896 3407 5344 8175
Size: 190 2706 511 1937 2831
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 345 379 3453 3487 4899 4933 8175
Size: 345 34 3074 34 1412 34 3242
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 4994 8175
Size: 4994 3181
BglI GCCn_nnn'nGGC
Cuts at: 0 658 1757 2695 5994 6773 8175
Size: 658 1099 938 3299 779 1402
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 4730 8175
Size: 4730 3445
BlpI GC'TnA_GC
Cuts at: 0 229 8175
Size: 229 7946
Bme1580I G_kGCm'C
Cuts at: 0 521 695 798 1025 1371 1555 1832 1922
Size: 521 174 103 227 346 184 277 90
Cuts at: 1922 2195 2249 2434 2623 3104 3304 3707 4007
Size: 273 54 185 189 481 200 403 300
Cuts at: 4007 4138 4377 4450 4535 4966 5695 5881 5949
Size: 131 239 73 85 431 729 186 68
Cuts at: 5949 6839 6854 6893 6942 7308 7334 7455 7927
Size: 890 15 39 49 366 26 121 472
Cuts at: 7927 8018 8175
Size: 91 157
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346 300 277 273 239 227 200 189
186 185 184 174 157 131 121 103
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26 15
BmgBI CAC'GTC
Cuts at: 0 1923 2394 5892 8175
Size: 1923 471 3498 2283
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 624 759 1819 4907 5304 8175
Size: 624 135 1060 3088 397 2871
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1848 4185 5247 6753 8175
Size: 1848 2337 1062 1506 1422
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1303 2050 3064 3514 4099 4262 5681
Size: 310 993 747 1014 450 585 163 1419
Cuts at: 5681 5789 6991 7231 7851 8175
Size: 108 1202 240 620 324
Fragments arranged by size:
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Bpu10I CC'TnA_GC
Cuts at: 0 190 971 2896 3407 4344 5344 5605 8175
Size: 190 781 1925 511 937 1000 261 2570
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1510 2320 3199 6457 7246 7839 8175
Size: 133 1377 810 879 3258 789 593 336
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 607 2093 3217 3768 4114 7152 8175
Size: 607 1486 1124 551 346 3038 1023
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1649 2190 3861 4790 5193 6039 6354 6888
Size: 1649 541 1671 929 403 846 315 534
Cuts at: 6888 8049 8175
Size: 1161 126
Fragments arranged by size:
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315 126
BsaBI GATnn'nnATC
Cuts at: 0 5509 8175
Size: 5509 2666
BsaHI Gr'CG_yC
Cuts at: 0 26 478 575 2123 3755 4253 4423 5279
Size: 26 452 97 1548 1632 498 170 856
Cuts at: 5279 6119 7109 7421 8175
Size: 840 990 312 754
Fragments arranged by size:
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312 170 97 26
BsaWI w'CCGG_w
Cuts at: 0 7558 7792 8175
Size: 7558 234 383
Fragments arranged by size:
7558 383 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 412 442 3970 4000 7674 7704
Size: 283 30 99 30 3528 30 3674 30
Cuts at: 7704 8175
Size: 471
Fragments arranged by size:
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30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 979 1573 3109 3973 5236 5239 5255
Size: 727 252 594 1536 864 1263 3 16
Cuts at: 5255 5320 5977 6637 7369 7842 7884 8053 8175
Size: 65 657 660 732 473 42 169 122
Fragments arranged by size:
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473 252 169 122 65 42 16 3
BseYI C'CCAG_C
Cuts at: 0 521 1033 1582 4356 4443 4518 4602 6924
Size: 521 512 549 2774 87 75 84 2322
Cuts at: 6924 7030 7504 7739 8175
Size: 106 474 235 436
Fragments arranged by size:
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106 87 84 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 919 1296 2105 2731 3751 3961 4197
Size: 400 519 377 809 626 1020 210 236
Cuts at: 4197 5398 5482 5724 6737 7312 7663 8175
Size: 1201 84 242 1013 575 351 512
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 3743 6863 8175
Size: 3743 3120 1312
Fragments arranged by size:
3743 3120 1312
BsiHKAI G_wGCw'C
Cuts at: 0 146 230 332 1058 1526 1922 3878 4345
Size: 146 84 102 726 468 396 1956 467
Cuts at: 4345 5069 5171 5393 6038 7172 7334 8018 8175
Size: 724 102 222 645 1134 162 684 157
Fragments arranged by size:
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396 222 162 157 146 102 102 84
BsmI GAATG_Cn'
Cuts at: 0 741 2953 7599 8175
Size: 741 2212 4646 576
Fragments arranged by size:
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BsmBI CGTCTCn'nnnn_
Cuts at: 0 4981 7116 7234 7246 7371 8175
Size: 4981 2135 118 12 125 804
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 146 203 230 332 521 695 798 1025
Size: 146 57 27 102 189 174 103 227
Cuts at: 1025 1058 1371 1526 1555 1699 1832 1922 2195
Size: 33 313 155 29 144 133 90 273
Cuts at: 2195 2249 2257 2351 2434 2623 2863 3104 3304
Size: 54 8 94 83 189 240 241 200
Cuts at: 3304 3707 3878 4007 4015 4138 4294 4345 4377
Size: 403 171 129 8 123 156 51 32
Cuts at: 4377 4450 4535 4966 5069 5171 5231 5393 5545
Size: 73 85 431 103 102 60 162 152
Cuts at: 5545 5695 5821 5881 5949 5969 6038 6314 6839
Size: 150 126 60 68 20 69 276 525
Cuts at: 6839 6854 6893 6942 7172 7262 7308 7334 7455
Size: 15 39 49 230 90 46 26 121
Cuts at: 7455 7544 7867 7927 8018 8175
Size: 89 323 60 91 157
Fragments arranged by size:
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240 230 227 200 189 189 174 171
162 157 156 155 152 150 146 144
133 129 126 123 121 103 103 102
102 94 91 90 90 89 85 83
73 69 68 60 60 60 57 54
51 49 46 39 33 32 29 27
26 20 15 8 8
BspEI T'CCGG_A
Cuts at: 0 7792 8175
Size: 7792 383
BspHI T'CATG_A
Cuts at: 0 4608 8175
Size: 4608 3567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 1332 3371 4422 4814 5746 6196 8175
Size: 185 1147 2039 1051 392 932 450 1979
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 2295 3061 4132 8175
Size: 2295 766 1071 4043
Fragments arranged by size:
4043 2295 1071 766
BsrDI GCAATG_nn'
Cuts at: 0 144 701 1173 1961 6207 7043 7715 8175
Size: 144 557 472 788 4246 836 672 460
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 28 1213 1256 1510 2038 2689 3023 4823
Size: 28 1185 43 254 528 651 334 1800
Cuts at: 4823 5017 5993 6385 6985 7558 7597 8175
Size: 194 976 392 600 573 39 578
Fragments arranged by size:
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392 334 254 194 43 39 28
BsrGI T'GTAC_A
Cuts at: 0 8072 8175
Size: 8072 103
BssSI C'ACGA_G
Cuts at: 0 340 2081 2969 3869 5218 7411 8175
Size: 340 1741 888 900 1349 2193 764
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 2411 3702 8175
Size: 492 1919 1291 4473
Fragments arranged by size:
4473 1919 1291 492
BstEII G'GTnAC_C
Cuts at: 0 1047 1638 3130 3804 6381 6759 8175
Size: 1047 591 1492 674 2577 378 1416
Fragments arranged by size:
2577 1492 1416 1047 674 591 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3334 3421 4458 5536 6488 7050 7417 7462
Size: 3334 87 1037 1078 952 562 367 45
Cuts at: 7462 7827 7911 8175
Size: 365 84 264
Fragments arranged by size:
3334 1078 1037 952 562 367 365 264
87 84 45
BstYI r'GATC_y
Cuts at: 0 107 503 2552 3572 3832 4730 4859 6005
Size: 107 396 2049 1020 260 898 129 1146
Cuts at: 6005 6089 6476 6694 7478 8175
Size: 84 387 218 784 697
Fragments arranged by size:
2049 1146 1020 898 784 697 396 387
260 218 129 107 84
Bsu36I CC'TnA_GG
Cuts at: 0 710 7667 8175
Size: 710 6957 508
Fragments arranged by size:
6957 710 508
BtgI C'CryG_G
Cuts at: 0 68 97 302 641 1140 1328 1895 3368
Size: 68 29 205 339 499 188 567 1473
Cuts at: 3368 4217 4941 5610 5684 5885 6876 7694 7820
Size: 849 724 669 74 201 991 818 126
Cuts at: 7820 7970 8175
Size: 150 205
Fragments arranged by size:
1473 991 849 818 724 669 567 499
339 205 205 201 188 150 126 74
68 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 59 195 819 1238 1892 6423 8175
Size: 59 136 624 419 654 4531 1752
Fragments arranged by size:
4531 1752 654 624 419 136 59
BtsI GCAGTG_nn'
Cuts at: 0 1202 2828 8175
Size: 1202 1626 5347
Fragments arranged by size:
5347 1626 1202
ClaI AT'CG_AT
Cuts at: 0 7433 8175
Size: 7433 742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1173 1208 6230 6265 6858 6893 7713 7748
Size: 1173 35 5022 35 593 35 820 35
Cuts at: 7748 8175
Size: 427
Fragments arranged by size:
5022 1173 820 593 427 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 1047 3138 3790 6042 6948 7749 8175
Size: 1047 2091 652 2252 906 801 426
Fragments arranged by size:
2252 2091 1047 906 801 652 426
DrdI GACnn_nn'nnGTC
Cuts at: 0 759 4252 7144 8140 8175
Size: 759 3493 2892 996 35
Fragments arranged by size:
3493 2892 996 759 35
EaeI y'GGCC_r
Cuts at: 0 1065 1172 1749 4416 4460 4821 5681 6879
Size: 1065 107 577 2667 44 361 860 1198
Cuts at: 6879 6983 8175
Size: 104 1192
Fragments arranged by size:
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104 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 265 3571 6435 8175
Size: 100 165 3306 2864 1740
Fragments arranged by size:
3306 2864 1740 165 100
EcoICRI GAG'CTC
Cuts at: 0 1524 5067 5391 8175
Size: 1524 3543 324 2784
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 400 971 1303 2050 2125 3064 3514
Size: 310 90 571 332 747 75 939 450
Cuts at: 3514 4099 4262 5255 5681 5789 5920 5998 6991
Size: 585 163 993 426 108 131 78 993
Cuts at: 6991 7231 7851 8175
Size: 240 620 324
Fragments arranged by size:
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426 332 324 310 240 163 131 108
90 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 3799 8175
Size: 85 3714 4376
Fragments arranged by size:
4376 3714 85
EcoO109I rG'GnC_Cy
Cuts at: 0 78 627 945 1732 1828 1829 2431 2619
Size: 78 549 318 787 96 1 602 188
Cuts at: 2619 2620 2844 2916 3100 3101 3300 3301 3769
Size: 1 224 72 184 1 199 1 468
Cuts at: 3769 3981 4143 4165 4373 4447 4532 4596 4963
Size: 212 162 22 208 74 85 64 367
Cuts at: 4963 5348 5691 5858 6645 6850 6851 7035 7057
Size: 385 343 167 787 205 1 184 22
Cuts at: 7057 7509 7607 7923 7924 8169 8175
Size: 452 98 316 1 245 6
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343 318 316 245 224 212 208 205
199 188 184 184 167 162 98 96
85 78 74 72 64 22 22 6
1 1 1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 3117 8175
Size: 3117 5058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2274 2880 4032 7980 8175
Size: 2274 606 1152 3948 195
Fragments arranged by size:
3948 2274 1152 606 195
FspI TGC'GCA
Cuts at: 0 6052 8175
Size: 6052 2123
HaeII r_GCGC'y
Cuts at: 0 168 578 4426 5125 5282 5985 6519 6690
Size: 168 410 3848 699 157 703 534 171
Cuts at: 6690 6818 7031 8175
Size: 128 213 1144
Fragments arranged by size:
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168 157 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 283 315 3562 3594 3817 3849
Size: 49 32 202 32 3247 32 223 32
Cuts at: 3849 5680 5712 7618 7650 8175
Size: 1831 32 1906 32 525
Fragments arranged by size:
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32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 4095 4257 5622 8175
Size: 4095 162 1365 2553
Fragments arranged by size:
4095 2553 1365 162
KasI G'GCGC_C
Cuts at: 0 574 4422 5278 8175
Size: 574 3848 856 2897
Fragments arranged by size:
3848 2897 856 574
KpnI G_GTAC'C
Cuts at: 0 5102 8175
Size: 5102 3073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 3560 8175
Size: 1385 2175 4615
Fragments arranged by size:
4615 2175 1385
MscI TGG'CCA
Cuts at: 0 1067 1174 1751 6881 8175
Size: 1067 107 577 5130 1294
Fragments arranged by size:
5130 1294 1067 577 107
MslI CAynn'nnrTG
Cuts at: 0 430 436 1798 2149 2527 2530 2578 2641
Size: 430 6 1362 351 378 3 48 63
Cuts at: 2641 2821 3907 4456 5467 5585 5785 5890 7048
Size: 180 1086 549 1011 118 200 105 1158
Cuts at: 7048 7415 7996 8002 8175
Size: 367 581 6 173
Fragments arranged by size:
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367 351 200 180 173 118 105 63
48 6 6 3
MspA1I CmG'CkG
Cuts at: 0 14 489 525 816 1310 1517 2062 2089
Size: 14 475 36 291 494 207 545 27
Cuts at: 2089 2180 2800 2889 4634 5247 5868 5928 6924
Size: 91 620 89 1745 613 621 60 996
Cuts at: 6924 7296 7647 8175
Size: 372 351 528
Fragments arranged by size:
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475 372 351 291 207 91 89 60
36 27 14
NaeI GCC'GGC
Cuts at: 0 30 1215 1258 1512 2691 3025 6987 7599
Size: 30 1185 43 254 1179 334 3962 612
Cuts at: 7599 8175
Size: 576
Fragments arranged by size:
3962 1185 1179 612 576 334 254 43
30
NarI GG'CG_CC
Cuts at: 0 575 4423 5279 8175
Size: 575 3848 856 2896
Fragments arranged by size:
3848 2896 856 575
NcoI C'CATG_G
Cuts at: 0 1328 4941 5610 7694 8175
Size: 1328 3613 669 2084 481
Fragments arranged by size:
3613 2084 1328 669 481
NgoMIV G'CCGG_C
Cuts at: 0 28 1213 1256 1510 2689 3023 6985 7597
Size: 28 1185 43 254 1179 334 3962 612
Cuts at: 7597 8175
Size: 578
Fragments arranged by size:
3962 1185 1179 612 578 334 254 43
28
NspI r_CATG'y
Cuts at: 0 246 1354 3087 5039 6070 6287 8175
Size: 246 1108 1733 1952 1031 217 1888
Fragments arranged by size:
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PasI CC'CwG_GG
Cuts at: 0 1113 2623 4285 4327 5536 7927 8175
Size: 1113 1510 1662 42 1209 2391 248
Fragments arranged by size:
2391 1662 1510 1209 1113 248 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1649 3055 4734 4947 5196 5294 5616
Size: 642 1007 1406 1679 213 249 98 322
Cuts at: 5616 6846 8175
Size: 1230 1329
Fragments arranged by size:
1679 1406 1329 1230 1007 642 322 249
213 98
PfoI T'CCnGG_A
Cuts at: 0 4239 4769 7124 7247 8175
Size: 4239 530 2355 123 928
Fragments arranged by size:
4239 2355 928 530 123
PmlI CAC'GTG
Cuts at: 0 1649 3861 5193 6039 6354 6888 8175
Size: 1649 2212 1332 846 315 534 1287
Fragments arranged by size:
2212 1649 1332 1287 846 534 315
PpuMI rG'GwC_Cy
Cuts at: 0 78 627 945 1732 2844 3981 4165 4596
Size: 78 549 318 787 1112 1137 184 431
Cuts at: 4596 5858 6645 7057 7509 8169 8175
Size: 1262 787 412 452 660 6
Fragments arranged by size:
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431 412 318 184 78 6
PshAI GACnn'nnGTC
Cuts at: 0 2132 4406 8175
Size: 2132 2274 3769
Fragments arranged by size:
3769 2274 2132
PspOMI G'GGCC_C
Cuts at: 0 1828 2430 2619 3100 3300 4373 4446 4531
Size: 1828 602 189 481 200 1073 73 85
Cuts at: 4531 4962 5691 6850 7923 8175
Size: 431 729 1159 1073 252
Fragments arranged by size:
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252 200 189 85 73
PspXI vC'TCGA_Gb
Cuts at: 0 220 310 319 7543 8175
Size: 220 90 9 7224 632
Fragments arranged by size:
7224 632 220 90 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 238 270 8056 8088 8175
Size: 238 32 7786 32 87
Fragments arranged by size:
7786 238 87 32 32
PstI C_TGCA'G
Cuts at: 0 182 881 1454 2374 2439 4408 4433 7981
Size: 182 699 573 920 65 1969 25 3548
Cuts at: 7981 8175
Size: 194
Fragments arranged by size:
3548 1969 920 699 573 194 182 65
25
PvuII CAG'CTG
Cuts at: 0 525 816 2089 5247 5868 5928 7296 7647
Size: 525 291 1273 3158 621 60 1368 351
Cuts at: 7647 8175
Size: 528
Fragments arranged by size:
3158 1368 1273 621 528 525 351 291
60
SacI G_AGCT'C
Cuts at: 0 1526 5069 5393 8175
Size: 1526 3543 324 2782
Fragments arranged by size:
3543 2782 1526 324
SanDI GG'GwC_CC
Cuts at: 0 627 2844 4165 6645 7509 8175
Size: 627 2217 1321 2480 864 666
Fragments arranged by size:
2480 2217 1321 864 666 627
SbfI CC_TGCA'GG
Cuts at: 0 4433 8175
Size: 4433 3742
ScaI AGT'ACT
Cuts at: 0 1609 8175
Size: 1609 6566
SexAI A'CCwGG_T
Cuts at: 0 789 5357 7131 8175
Size: 789 4568 1774 1044
Fragments arranged by size:
4568 1774 1044 789
SfcI C'TryA_G
Cuts at: 0 178 877 1450 2370 2435 3645 4404 4429
Size: 178 699 573 920 65 1210 759 25
Cuts at: 4429 5775 6819 7872 7977 8175
Size: 1346 1044 1053 105 198
Fragments arranged by size:
1346 1210 1053 1044 920 759 699 573
198 178 105 65 25
SfoI GGC'GCC
Cuts at: 0 576 4424 5280 8175
Size: 576 3848 856 2895
Fragments arranged by size:
3848 2895 856 576
SmaI CCC'GGG
Cuts at: 0 655 3209 4378 5813 7018 7309 7327 8175
Size: 655 2554 1169 1435 1205 291 18 848
Fragments arranged by size:
2554 1435 1205 1169 848 655 291 18
SmlI C'TyrA_G
Cuts at: 0 148 220 310 319 1525 2335 3214 6472
Size: 148 72 90 9 1206 810 879 3258
Cuts at: 6472 7261 7543 7854 8175
Size: 789 282 311 321
Fragments arranged by size:
3258 1206 879 810 789 321 311 282
148 90 72 9
StuI AGG'CCT
Cuts at: 0 622 1448 2014 2333 4076 4643 5495 5603
Size: 622 826 566 319 1743 567 852 108
Cuts at: 5603 7942 8175
Size: 2339 233
Fragments arranged by size:
2339 1743 852 826 622 567 566 319
233 108
StyI C'CwwG_G
Cuts at: 0 773 1025 1328 1457 1502 2681 2729 3188
Size: 773 252 303 129 45 1179 48 459
Cuts at: 3188 3260 4146 4941 5009 5610 5699 6020 7637
Size: 72 886 795 68 601 89 321 1617
Cuts at: 7637 7694 7769 8175
Size: 57 75 406
Fragments arranged by size:
1617 1179 886 795 773 601 459 406
321 303 252 129 89 75 72 68
57 48 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 2677 4048 4513 4939 5496 6646 6916
Size: 187 2490 1371 465 426 557 1150 270
Cuts at: 6916 8175
Size: 1259
Fragments arranged by size:
2490 1371 1259 1150 557 465 426 270
187
TatI w'GTAC_w
Cuts at: 0 1607 2495 2792 3383 3944 4223 8072 8175
Size: 1607 888 297 591 561 279 3849 103
Fragments arranged by size:
3849 1607 888 591 561 297 279 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 1368 1400 3776 3808 7674 7706
Size: 82 32 1254 32 2376 32 3866 32
Cuts at: 7706 7801 7833 8175
Size: 95 32 342
Fragments arranged by size:
3866 2376 1254 342 95 82 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2848 7845 8175
Size: 2848 4997 330
Fragments arranged by size:
4997 2848 330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 639 3528 4674 7644 7827 8001 8175
Size: 639 2889 1146 2970 183 174 174
Fragments arranged by size:
2970 2889 1146 639 183 174 174
XhoI C'TCGA_G
Cuts at: 0 220 310 319 7543 8175
Size: 220 90 9 7224 632
Fragments arranged by size:
7224 632 220 90 9
XmaI C'CCGG_G
Cuts at: 0 653 3207 4376 5811 7016 7307 7325 8175
Size: 653 2554 1169 1435 1205 291 18 850
Fragments arranged by size:
2554 1435 1205 1169 850 653 291 18
XmnI GAAnn'nnTTC
Cuts at: 0 121 7963 8175
Size: 121 7842 212
Fragments arranged by size:
7842 212 121
ZraI GAC'GTC
Cuts at: 0 4254 8175
Size: 4254 3921
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuII SacI SanDI SbfI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI AscI AseI AsiSI AvrII
BaeI BciVI BfrBI BmtI BsiWI BssHII BstBI BstZ17I
DraI EagI EarI EcoRI FseI FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PsiI PvuI RsrII SacII SalI
SapI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI