(Linear) (Six-base) MAPSORT of: KIEE2359.seq  Check: 8124  from: 1  to: 1161



KIEE2359.orf

 With 182 enzymes: SgfI * 

                        June 23, 2011 13:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     592     864    1161 
   Size:        592     272     297

  Fragments arranged by size: 

                 592     297     272
 
AatII G_ACGT'C

Cuts at:      0     302    1161 
   Size:        302     859
 
AfeI AGC'GCT

Cuts at:      0     997    1161 
   Size:        997     164
 
AflIII A'CryG_T

Cuts at:      0    1065    1161 
   Size:       1065      96
 
AseI AT'TA_AT

Cuts at:      0     418    1052    1161 
   Size:        418     634     109

  Fragments arranged by size: 

                 634     418     109
 
AvaI C'yCGr_G

Cuts at:      0    1111    1161 
   Size:       1111      50
 
BamHI G'GATC_C

Cuts at:      0     843    1057    1161 
   Size:        843     214     104

  Fragments arranged by size: 

                 843     214     104
 
BanI G'GyrC_C

Cuts at:      0     229     816    1161 
   Size:        229     587     345

  Fragments arranged by size: 

                 587     345     229
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     546    1011    1161 
   Size:        546     465     150

  Fragments arranged by size: 

                 546     465     150
 
Bme1580I G_kGCm'C

Cuts at:      0     819    1161 
   Size:        819     342
 
BmgBI CAC'GTC

Cuts at:      0     961    1161 
   Size:        961     200
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     734    1161 
   Size:        734     427
 
Bpu10I CC'TnA_GC

Cuts at:      0     148     656    1161 
   Size:        148     508     505

  Fragments arranged by size: 

                 508     505     148
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     754    1161 
   Size:        754     407
 
BsaAI yAC'GTr

Cuts at:      0     545    1161 
   Size:        545     616
 
BsaBI GATnn'nnATC

Cuts at:      0      97    1161 
   Size:         97    1064
 
BsaHI Gr'CG_yC

Cuts at:      0     299    1161 
   Size:        299     862
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     607     693    1161 
   Size:         46     561      86     468

  Fragments arranged by size: 

                 561     468      86      46
 
BseYI C'CCAG_C

Cuts at:      0     134     423     434     812    1161 
   Size:        134     289      11     378     349

  Fragments arranged by size: 

                 378     349     289     134      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1072    1161 
   Size:       1072      89
 
Bsp1286I G_dGCh'C

Cuts at:      0     819    1161 
   Size:        819     342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     119     592     864    1161 
   Size:        119     473     272     297

  Fragments arranged by size: 

                 473     297     272     119
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     240     840    1161 
   Size:        240     600     321

  Fragments arranged by size: 

                 600     321     240
 
BstYI r'GATC_y

Cuts at:      0     195     674     843    1057    1161 
   Size:        195     479     169     214     104

  Fragments arranged by size: 

                 479     214     195     169     104
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     396     730    1161 
   Size:        396     334     431

  Fragments arranged by size: 

                 431     396     334
 
ClaI AT'CG_AT

Cuts at:      0     319    1161 
   Size:        319     842
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     862    1161 
   Size:        862     299
 
EaeI y'GGCC_r

Cuts at:      0      95    1161 
   Size:         95    1066
 
FspI TGC'GCA

Cuts at:      0     409    1161 
   Size:        409     752
 
HaeII r_GCGC'y

Cuts at:      0     999    1161 
   Size:        999     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419    1126    1158    1161 
   Size:        387      32     707      32       3

  Fragments arranged by size: 

                 707     387      32      32       3
 
HincII GTy'rAC

Cuts at:      0     564    1161 
   Size:        564     597
 
MscI TGG'CCA

Cuts at:      0      97    1161 
   Size:         97    1064
 
MslI CAynn'nnrTG

Cuts at:      0     790    1161 
   Size:        790     371
 
NspI r_CATG'y

Cuts at:      0     667    1069    1161 
   Size:        667     402      92

  Fragments arranged by size: 

                 667     402      92
 
PasI CC'CwG_GG

Cuts at:      0     868    1161 
   Size:        868     293
 
PciI A'CATG_T

Cuts at:      0    1065    1161 
   Size:       1065      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     378    1161 
   Size:        378     783
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555    1161 
   Size:        523      32     606

  Fragments arranged by size: 

                 606     523      32
 
SfcI C'TryA_G

Cuts at:      0     384    1161 
   Size:        384     777
 
SmlI C'TyrA_G

Cuts at:      0    1111    1161 
   Size:       1111      50
 
SnaBI TAC'GTA

Cuts at:      0     545    1161 
   Size:        545     616
 
SphI G_CATG'C

Cuts at:      0     667    1161 
   Size:        667     494
 
SspI AAT'ATT

Cuts at:      0     837    1161 
   Size:        837     324
 
StuI AGG'CCT

Cuts at:      0     717    1161 
   Size:        717     444
 
TatI w'GTAC_w

Cuts at:      0    1068    1161 
   Size:       1068      93
 
XhoI C'TCGA_G

Cuts at:      0    1111    1161 
   Size:       1111      50
 
XmnI GAAnn'nnTTC

Cuts at:      0    1008    1161 
   Size:       1008     153
 
ZraI GAC'GTC

Cuts at:      0     300    1161 
   Size:        300     861
 

 Enzymes that do cut:

     AarI    AatII     AfeI   AflIII     AseI     AvaI    BamHI     BanI
     BbsI Bme1580I    BmgBI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BseRI    BseYI     BsgI Bsp1286I    BspMI    BstXI    BstYI
    BtgZI     ClaI     DrdI     EaeI     FspI    HaeII    Hin4I   HincII
     MscI     MslI     NspI     PasI     PciI    PflMI     PpiI     SfcI
     SmlI    SnaBI     SphI     SspI     StuI     TatI     XhoI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI    AsiSI
    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BpmI    BpuEI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SrfI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XmaI