Restriction map for
pF1KE2360
(Linear) (Six-base) MAPSORT of: KIEE2360.seq Check: 2122 from: 1 to: 6219
KIEE2360.orf
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3633 6219
Size: 3633 2586
AccI GT'mk_AC
Cuts at: 0 256 1250 3351 4543 6219
Size: 256 994 2101 1192 1676
Fragments arranged by size:
2101 1676 1192 994 256
Acc65I G'GTAC_C
Cuts at: 0 2277 6219
Size: 2277 3942
AclI AA'CG_TT
Cuts at: 0 1095 5130 6219
Size: 1095 4035 1089
Fragments arranged by size:
4035 1095 1089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3112 3364 4084 4178 4226 4367 4388 5274
Size: 3112 252 720 94 48 141 21 886
Cuts at: 5274 6219
Size: 945
Fragments arranged by size:
3112 945 886 720 252 141 94 48
21
AfeI AGC'GCT
Cuts at: 0 3097 6219
Size: 3097 3122
AflIII A'CryG_T
Cuts at: 0 4362 4430 5954 6150 6219
Size: 4362 68 1524 196 69
Fragments arranged by size:
4362 1524 196 69 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 5882 6219
Size: 265 5617 337
Fragments arranged by size:
5617 337 265
AlwNI CAG_nnn'CTG
Cuts at: 0 2071 2322 4204 4912 6219
Size: 2071 251 1882 708 1307
Fragments arranged by size:
2071 1882 1307 708 251
ApaI G_GGCC'C
Cuts at: 0 288 6219
Size: 288 5931
ApaLI G'TGCA_C
Cuts at: 0 1525 2482 6219
Size: 1525 957 3737
Fragments arranged by size:
3737 1525 957
ApoI r'AATT_y
Cuts at: 0 16 636 1391 1421 1494 2005 2488 3202
Size: 16 620 755 30 73 511 483 714
Cuts at: 3202 5243 5381 5965 6054 6219
Size: 2041 138 584 89 165
Fragments arranged by size:
2041 755 714 620 584 511 483 165
138 89 73 30 16
AseI AT'TA_AT
Cuts at: 0 4294 5729 6219
Size: 4294 1435 490
Fragments arranged by size:
4294 1435 490
AvaI C'yCGr_G
Cuts at: 0 2954 2971 6219
Size: 2954 17 3248
Fragments arranged by size:
3248 2954 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3481 3514 4498 4531 6219
Size: 3481 33 984 33 1688
Fragments arranged by size:
3481 1688 984 33 33
BanI G'GyrC_C
Cuts at: 0 2277 6219
Size: 2277 3942
BanII G_rGCy'C
Cuts at: 0 59 91 125 288 1184 1291 1330 2546
Size: 59 32 34 163 896 107 39 1216
Cuts at: 2546 3929 4850 6219
Size: 1383 921 1369
Fragments arranged by size:
1383 1369 1216 921 896 163 107 59
39 34 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 1005 1691 5557 6119 6219
Size: 449 556 686 3866 562 100
Fragments arranged by size:
3866 686 562 556 449 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 3813 5933 6219
Size: 3813 2120 286
Fragments arranged by size:
3813 2120 286
BfrBI ATG'CAT
Cuts at: 0 933 3445 5259 6123 6219
Size: 933 2512 1814 864 96
Fragments arranged by size:
2512 1814 933 864 96
BglI GCCn_nnn'nGGC
Cuts at: 0 1325 6219
Size: 1325 4894
BglII A'GATC_T
Cuts at: 0 186 2472 6219
Size: 186 2286 3747
Fragments arranged by size:
3747 2286 186
BlpI GC'TnA_GC
Cuts at: 0 31 53 4818 6219
Size: 31 22 4765 1401
Fragments arranged by size:
4765 1401 31 22
Bme1580I G_kGCm'C
Cuts at: 0 288 697 1529 1820 2040 2486 6219
Size: 288 409 832 291 220 446 3733
Fragments arranged by size:
3733 832 446 409 291 288 220
BmgBI CAC'GTC
Cuts at: 0 5811 6219
Size: 5811 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 3801 6219
Size: 3801 2418
BmtI G_CTAG'C
Cuts at: 0 3086 5918 6219
Size: 3086 2832 301
Fragments arranged by size:
3086 2832 301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 1661 2798 3736 6219
Size: 381 1280 1137 938 2483
Fragments arranged by size:
2483 1280 1137 938 381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2243 4792 5107 6219
Size: 2243 2549 315 1112
Fragments arranged by size:
2549 2243 1112 315
Bpu10I CC'TnA_GC
Cuts at: 0 4849 6219
Size: 4849 1370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1598 1678 2453 5192 5409 6219
Size: 709 889 80 775 2739 217 810
Fragments arranged by size:
2739 889 810 775 709 217 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 396 6219
Size: 396 5823
BsaAI yAC'GTr
Cuts at: 0 339 5691 6219
Size: 339 5352 528
Fragments arranged by size:
5352 528 339
BsaBI GATnn'nnATC
Cuts at: 0 2729 3296 4316 6219
Size: 2729 567 1020 1903
Fragments arranged by size:
2729 1903 1020 567
BsaHI Gr'CG_yC
Cuts at: 0 671 6219
Size: 671 5548
BsaWI w'CCGG_w
Cuts at: 0 936 6219
Size: 936 5283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 6219
Size: 772 30 5417
Fragments arranged by size:
5417 772 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1004 1637 2771 5790 6219
Size: 1004 633 1134 3019 429
Fragments arranged by size:
3019 1134 1004 633 429
BseYI C'CCAG_C
Cuts at: 0 2789 5047 6219
Size: 2789 2258 1172
Fragments arranged by size:
2789 2258 1172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1496 2658 3590 4452 4490 5878 6219
Size: 271 1225 1162 932 862 38 1388 341
Fragments arranged by size:
1388 1225 1162 932 862 341 271 38
BsiHKAI G_wGCw'C
Cuts at: 0 59 1529 2486 2546 2781 3649 3929 4850
Size: 59 1470 957 60 235 868 280 921
Cuts at: 4850 6219
Size: 1369
Fragments arranged by size:
1470 1369 957 921 868 280 235 60
59
BsmI GAATG_Cn'
Cuts at: 0 1577 6123 6219
Size: 1577 4546 96
Fragments arranged by size:
4546 1577 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 561 844 6219
Size: 561 283 5375
Fragments arranged by size:
5375 561 283
Bsp1286I G_dGCh'C
Cuts at: 0 59 91 125 288 697 1184 1291 1330
Size: 59 32 34 163 409 487 107 39
Cuts at: 1330 1529 1820 2040 2486 2546 2781 3649 3929
Size: 199 291 220 446 60 235 868 280
Cuts at: 3929 4850 6219
Size: 921 1369
Fragments arranged by size:
1369 921 868 487 446 409 291 280
235 220 199 163 107 60 59 39
34 32
BspEI T'CCGG_A
Cuts at: 0 936 6219
Size: 936 5283
BspHI T'CATG_A
Cuts at: 0 2588 6219
Size: 2588 3631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2070 3535 3633 5850 6219
Size: 2070 1465 98 2217 369
Fragments arranged by size:
2217 2070 1465 369 98
BsrBI CCG'CTC
Cuts at: 0 6006 6219
Size: 6006 213
BsrDI GCAATG_nn'
Cuts at: 0 1988 2392 2499 3371 5039 5984 6219
Size: 1988 404 107 872 1668 945 235
Fragments arranged by size:
1988 1668 945 872 404 235 107
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2322 4056 6219
Size: 2322 1734 2163
Fragments arranged by size:
2322 2163 1734
BstBI TT'CG_AA
Cuts at: 0 378 4068 6219
Size: 378 3690 2151
Fragments arranged by size:
3690 2151 378
BstYI r'GATC_y
Cuts at: 0 186 2472 2579 3171 6219
Size: 186 2286 107 592 3048
Fragments arranged by size:
3048 2286 592 186 107
BstZ17I GTA'TAC
Cuts at: 0 4544 6219
Size: 4544 1675
Bsu36I CC'TnA_GG
Cuts at: 0 2783 3176 3920 6219
Size: 2783 393 744 2299
Fragments arranged by size:
2783 2299 744 393
BtgI C'CryG_G
Cuts at: 0 92 2573 2678 6219
Size: 92 2481 105 3541
Fragments arranged by size:
3541 2481 105 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4930 6219
Size: 4930 1289
BtsI GCAGTG_nn'
Cuts at: 0 2998 4757 6219
Size: 2998 1759 1462
Fragments arranged by size:
2998 1759 1462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1158 1193 6219
Size: 1158 35 5026
Fragments arranged by size:
5026 1158 35
DraI TTT'AAA
Cuts at: 0 2558 4271 4460 5735 6219
Size: 2558 1713 189 1275 484
Fragments arranged by size:
2558 1713 1275 484 189
DraIII CAC_nnn'GTG
Cuts at: 0 1474 4250 6219
Size: 1474 2776 1969
Fragments arranged by size:
2776 1969 1474
EaeI y'GGCC_r
Cuts at: 0 2 1304 2915 4281 4754 4874 5307 6219
Size: 2 1302 1611 1366 473 120 433 912
Fragments arranged by size:
1611 1366 1302 912 473 433 120 2
EarI CTCTTCn'nnn_
Cuts at: 0 951 1296 1335 1862 2629 2927 3383 4070
Size: 951 345 39 527 767 298 456 687
Cuts at: 4070 4416 5069 5228 5692 6219
Size: 346 653 159 464 527
Fragments arranged by size:
951 767 687 653 527 527 464 456
346 345 298 159 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2322 6219
Size: 2322 3897
EcoICRI GAG'CTC
Cuts at: 0 57 2544 3927 4848 6219
Size: 57 2487 1383 921 1371
Fragments arranged by size:
2487 1383 1371 921 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2243 3112 3364 4084 4178 4226 4367 4388
Size: 2243 869 252 720 94 48 141 21
Cuts at: 4388 4792 5107 5274 6219
Size: 404 315 167 945
Fragments arranged by size:
2243 945 869 720 404 315 252 167
141 94 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4563 6219
Size: 4563 1656
EcoO109I rG'GnC_Cy
Cuts at: 0 284 5598 6219
Size: 284 5314 621
Fragments arranged by size:
5314 621 284
EcoRI G'AATT_C
Cuts at: 0 2005 6219
Size: 2005 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1639 2772 3354 5538 6219
Size: 1639 1133 582 2184 681
Fragments arranged by size:
2184 1639 1133 681 582
FspI TGC'GCA
Cuts at: 0 13 6219
Size: 13 6206
HaeII r_GCGC'y
Cuts at: 0 3099 6219
Size: 3099 3120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 405 437 448 451 480 483
Size: 219 32 154 32 11 3 29 3
Cuts at: 483 918 950 992 1024 2359 2391 2839 2871
Size: 435 32 42 32 1335 32 448 32
Cuts at: 2871 2967 2999 3136 3168 4030 4062 4219 4251
Size: 96 32 137 32 862 32 157 32
Cuts at: 4251 4373 4405 4557 4589 6219
Size: 122 32 152 32 1630
Fragments arranged by size:
1630 1335 862 448 435 219 157 154
152 137 122 96 42 32 32 32
32 32 32 32 32 32 32 32
32 29 11 3 3
HincII GTy'rAC
Cuts at: 0 238 6219
Size: 238 5981
HindIII A'AGCT_T
Cuts at: 0 1672 2947 5791 6219
Size: 1672 1275 2844 428
Fragments arranged by size:
2844 1672 1275 428
KpnI G_GTAC'C
Cuts at: 0 2281 6219
Size: 2281 3938
MfeI C'AATT_G
Cuts at: 0 1221 3493 5741 6219
Size: 1221 2272 2248 478
Fragments arranged by size:
2272 2248 1221 478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 2784 2909 3492 5853 6219
Size: 1019 1765 125 583 2361 366
Fragments arranged by size:
2361 1765 1019 583 366 125
MscI TGG'CCA
Cuts at: 0 1306 2917 4283 4756 4876 5309 6219
Size: 1306 1611 1366 473 120 433 910
Fragments arranged by size:
1611 1366 1306 910 473 433 120
MslI CAynn'nnrTG
Cuts at: 0 1928 2641 2654 5069 6219
Size: 1928 713 13 2415 1150
Fragments arranged by size:
2415 1928 1150 713 13
MspA1I CmG'CkG
Cuts at: 0 2322 2508 2793 3282 6219
Size: 2322 186 285 489 2937
Fragments arranged by size:
2937 2322 489 285 186
NcoI C'CATG_G
Cuts at: 0 2573 2678 6219
Size: 2573 105 3541
Fragments arranged by size:
3541 2573 105
NdeI CA'TA_TG
Cuts at: 0 1812 5883 6219
Size: 1812 4071 336
Fragments arranged by size:
4071 1812 336
NheI G'CTAG_C
Cuts at: 0 3082 5914 6219
Size: 3082 2832 305
Fragments arranged by size:
3082 2832 305
NsiI A_TGCA'T
Cuts at: 0 935 3447 5261 6125 6219
Size: 935 2512 1814 864 94
Fragments arranged by size:
2512 1814 935 864 94
NspI r_CATG'y
Cuts at: 0 933 2482 2572 3445 4366 4434 5958 5981
Size: 933 1549 90 873 921 68 1524 23
Cuts at: 5981 6154 6219
Size: 173 65
Fragments arranged by size:
1549 1524 933 921 873 173 90 68
65 23
PciI A'CATG_T
Cuts at: 0 4362 4430 5954 6150 6219
Size: 4362 68 1524 196 69
Fragments arranged by size:
4362 1524 196 69 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 6219
Size: 753 5466
PfoI T'CCnGG_A
Cuts at: 0 2009 6219
Size: 2009 4210
PmlI CAC'GTG
Cuts at: 0 339 6219
Size: 339 5880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5348 5380 6219
Size: 5348 32 839
Fragments arranged by size:
5348 839 32
PsiI TTA'TAA
Cuts at: 0 3421 6219
Size: 3421 2798
PspOMI G'GGCC_C
Cuts at: 0 284 6219
Size: 284 5935
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 6219
Size: 1760 32 4427
Fragments arranged by size:
4427 1760 32
PstI C_TGCA'G
Cuts at: 0 1739 2067 2847 3281 5781 6219
Size: 1739 328 780 434 2500 438
Fragments arranged by size:
2500 1739 780 438 434 328
PvuII CAG'CTG
Cuts at: 0 2322 2508 2793 6219
Size: 2322 186 285 3426
Fragments arranged by size:
3426 2322 285 186
SacI G_AGCT'C
Cuts at: 0 59 2546 3929 4850 6219
Size: 59 2487 1383 921 1369
Fragments arranged by size:
2487 1383 1369 921 59
SapI GCTCTTCn'nnn_
Cuts at: 0 1296 1335 3383 4070 6219
Size: 1296 39 2048 687 2149
Fragments arranged by size:
2149 2048 1296 687 39
SbfI CC_TGCA'GG
Cuts at: 0 2067 6219
Size: 2067 4152
ScaI AGT'ACT
Cuts at: 0 2878 4223 6219
Size: 2878 1345 1996
Fragments arranged by size:
2878 1996 1345
SfcI C'TryA_G
Cuts at: 0 321 1453 1735 2063 2843 3072 3277 4576
Size: 321 1132 282 328 780 229 205 1299
Cuts at: 4576 5777 6219
Size: 1201 442
Fragments arranged by size:
1299 1201 1132 780 442 328 321 282
229 205
SmlI C'TyrA_G
Cuts at: 0 724 1613 1693 2468 5207 5388 6219
Size: 724 889 80 775 2739 181 831
Fragments arranged by size:
2739 889 831 775 724 181 80
SphI G_CATG'C
Cuts at: 0 933 2572 5981 6219
Size: 933 1639 3409 238
Fragments arranged by size:
3409 1639 933 238
SspI AAT'ATT
Cuts at: 0 1851 1894 2554 2831 6219
Size: 1851 43 660 277 3388
Fragments arranged by size:
3388 1851 660 277 43
StyI C'CwwG_G
Cuts at: 0 888 1557 1976 2573 2678 6219
Size: 888 669 419 597 105 3541
Fragments arranged by size:
3541 888 669 597 419 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1182 4055 5569 6200 6219
Size: 1182 2873 1514 631 19
Fragments arranged by size:
2873 1514 1182 631 19
TatI w'GTAC_w
Cuts at: 0 2876 4221 5342 6219
Size: 2876 1345 1121 877
Fragments arranged by size:
2876 1345 1121 877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2925 6219
Size: 2925 3294
XmnI GAAnn'nnTTC
Cuts at: 0 413 559 1424 1688 1775 6219
Size: 413 146 865 264 87 4444
Fragments arranged by size:
4444 865 413 264 146 87
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI
BanII BbsI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NheI NsiI NspI PciI PflMI PfoI
PmlI PpiI PsiI PspOMI PsrI PstI PvuII SacI
SapI SbfI ScaI SfcI SmlI SphI SspI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AscI AsiSI
AvrII BamHI BbeI BbvCI BcgI BclI BsiEI BsiWI
BsrFI BsrGI BssHII BssSI BstEII BstXI ClaI DrdI
EagI EcoRV FseI FspAI HpaI KasI MluI NaeI
NarI NgoMIV NotI NruI PacI PasI PmeI PpuMI
PshAI PspXI PvuI RsrII SacII SalI SanDI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI