Restriction map for
pF1KE2363
(Linear) (Six-base) MAPSORT of: KIEE2363.seq Check: 188 from: 1 to: 1551
KIEE2363.orf
With 182 enzymes: SgfI *
July 21, 2011 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 1438 1551
Size: 724 714 113
Fragments arranged by size:
724 714 113
AccI GT'mk_AC
Cuts at: 0 350 1551
Size: 350 1201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1551
Size: 227 1324
AflII C'TTAA_G
Cuts at: 0 1495 1551
Size: 1495 56
AgeI A'CCGG_T
Cuts at: 0 781 1551
Size: 781 770
AleI CACnn'nnGTG
Cuts at: 0 793 1551
Size: 793 758
AlwNI CAG_nnn'CTG
Cuts at: 0 1269 1551
Size: 1269 282
AvaI C'yCGr_G
Cuts at: 0 248 364 1006 1349 1551
Size: 248 116 642 343 202
Fragments arranged by size:
642 343 248 202 116
AvrII C'CTAG_G
Cuts at: 0 1110 1551
Size: 1110 441
BanI G'GyrC_C
Cuts at: 0 15 278 1551
Size: 15 263 1273
Fragments arranged by size:
1273 263 15
BanII G_rGCy'C
Cuts at: 0 524 871 1551
Size: 524 347 680
Fragments arranged by size:
680 524 347
BbeI G_GCGC'C
Cuts at: 0 282 1551
Size: 282 1269
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 1551
Size: 160 1391
BglI GCCn_nnn'nGGC
Cuts at: 0 38 1551
Size: 38 1513
BglII A'GATC_T
Cuts at: 0 1247 1551
Size: 1247 304
BlpI GC'TnA_GC
Cuts at: 0 28 1551
Size: 28 1523
Bme1580I G_kGCm'C
Cuts at: 0 200 1551
Size: 200 1351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 871 1372 1551
Size: 19 852 501 179
Fragments arranged by size:
852 501 179 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1551
Size: 820 731
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 1551
Size: 361 1190
BsaAI yAC'GTr
Cuts at: 0 201 1551
Size: 201 1350
BsaBI GATnn'nnATC
Cuts at: 0 227 1551
Size: 227 1324
BsaHI Gr'CG_yC
Cuts at: 0 279 1551
Size: 279 1272
BsaWI w'CCGG_w
Cuts at: 0 781 1551
Size: 781 770
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 48 1141 1258 1306 1551
Size: 48 1093 117 48 245
Fragments arranged by size:
1093 245 117 48 48
BseYI C'CCAG_C
Cuts at: 0 452 749 855 871 1067 1479 1551
Size: 452 297 106 16 196 412 72
Fragments arranged by size:
452 412 297 196 106 72 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1282 1551
Size: 1237 45 269
Fragments arranged by size:
1237 269 45
Bsp1286I G_dGCh'C
Cuts at: 0 200 524 871 1551
Size: 200 324 347 680
Fragments arranged by size:
680 347 324 200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 1438 1551
Size: 724 714 113
Fragments arranged by size:
724 714 113
BsrBI CCG'CTC
Cuts at: 0 300 339 1551
Size: 300 39 1212
Fragments arranged by size:
1212 300 39
BsrFI r'CCGG_y
Cuts at: 0 305 781 1551
Size: 305 476 770
Fragments arranged by size:
770 476 305
BstEII G'GTnAC_C
Cuts at: 0 211 1521 1551
Size: 211 1310 30
Fragments arranged by size:
1310 211 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 795 1551
Size: 486 309 756
Fragments arranged by size:
756 486 309
BstYI r'GATC_y
Cuts at: 0 1247 1353 1551
Size: 1247 106 198
Fragments arranged by size:
1247 198 106
Bsu36I CC'TnA_GG
Cuts at: 0 1079 1551
Size: 1079 472
BtgI C'CryG_G
Cuts at: 0 274 485 550 908 1016 1551
Size: 274 211 65 358 108 535
Fragments arranged by size:
535 358 274 211 108 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 1551
Size: 729 822
BtsI GCAGTG_nn'
Cuts at: 0 922 1551
Size: 922 629
EaeI y'GGCC_r
Cuts at: 0 492 537 740 884 1128 1551
Size: 492 45 203 144 244 423
Fragments arranged by size:
492 423 244 203 144 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 1175 1551
Size: 250 925 376
Fragments arranged by size:
925 376 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 227 871 1372 1551
Size: 19 208 644 501 179
Fragments arranged by size:
644 501 208 179 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 673 1551
Size: 673 878
EcoO109I rG'GnC_Cy
Cuts at: 0 269 1075 1551
Size: 269 806 476
Fragments arranged by size:
806 476 269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 1551
Size: 174 1377
HaeII r_GCGC'y
Cuts at: 0 282 1551
Size: 282 1269
HincII GTy'rAC
Cuts at: 0 171 765 1551
Size: 171 594 786
Fragments arranged by size:
786 594 171
KasI G'GCGC_C
Cuts at: 0 278 1551
Size: 278 1273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 854 1551
Size: 559 295 697
Fragments arranged by size:
697 559 295
MscI TGG'CCA
Cuts at: 0 494 539 1130 1551
Size: 494 45 591 421
Fragments arranged by size:
591 494 421 45
MslI CAynn'nnrTG
Cuts at: 0 430 484 758 793 1551
Size: 430 54 274 35 758
Fragments arranged by size:
758 430 274 54 35
MspA1I CmG'CkG
Cuts at: 0 114 820 1551
Size: 114 706 731
Fragments arranged by size:
731 706 114
NaeI GCC'GGC
Cuts at: 0 307 1551
Size: 307 1244
NarI GG'CG_CC
Cuts at: 0 279 1551
Size: 279 1272
NcoI C'CATG_G
Cuts at: 0 274 485 1551
Size: 274 211 1066
Fragments arranged by size:
1066 274 211
NgoMIV G'CCGG_C
Cuts at: 0 305 1551
Size: 305 1246
NspI r_CATG'y
Cuts at: 0 907 1551
Size: 907 644
PasI CC'CwG_GG
Cuts at: 0 694 1551
Size: 694 857
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 1551
Size: 756 795
PfoI T'CCnGG_A
Cuts at: 0 967 1551
Size: 967 584
PmlI CAC'GTG
Cuts at: 0 201 1551
Size: 201 1350
PpuMI rG'GwC_Cy
Cuts at: 0 269 1551
Size: 269 1282
PvuII CAG'CTG
Cuts at: 0 114 820 1551
Size: 114 706 731
Fragments arranged by size:
731 706 114
SanDI GG'GwC_CC
Cuts at: 0 269 1551
Size: 269 1282
SexAI A'CCwGG_T
Cuts at: 0 1318 1551
Size: 1318 233
SfoI GGC'GCC
Cuts at: 0 280 1551
Size: 280 1271
SmaI CCC'GGG
Cuts at: 0 250 1351 1551
Size: 250 1101 200
Fragments arranged by size:
1101 250 200
SmlI C'TyrA_G
Cuts at: 0 364 835 1006 1495 1551
Size: 364 471 171 489 56
Fragments arranged by size:
489 471 364 171 56
SphI G_CATG'C
Cuts at: 0 907 1551
Size: 907 644
StuI AGG'CCT
Cuts at: 0 1501 1551
Size: 1501 50
StyI C'CwwG_G
Cuts at: 0 274 371 407 485 563 1110 1551
Size: 274 97 36 78 78 547 441
Fragments arranged by size:
547 441 274 97 78 78 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 847 1081 1393 1471 1551
Size: 847 234 312 78 80
Fragments arranged by size:
847 312 234 80 78
TatI w'GTAC_w
Cuts at: 0 1370 1551
Size: 1370 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 817 879 1326 1441 1551
Size: 817 62 447 115 110
Fragments arranged by size:
817 447 115 110 62
XhoI C'TCGA_G
Cuts at: 0 364 1006 1551
Size: 364 642 545
Fragments arranged by size:
642 545 364
XmaI C'CCGG_G
Cuts at: 0 248 1349 1551
Size: 248 1101 202
Fragments arranged by size:
1101 248 202
XmnI GAAnn'nnTTC
Cuts at: 0 157 388 1551
Size: 157 231 1163
Fragments arranged by size:
1163 231 157
Enzymes that do cut:
AarI AccI AcuI AflII AgeI AleI AlwNI AvaI
AvrII BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrFI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EciI
Eco57MI EcoNI EcoO109I FalI HaeII HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpuMI PvuII SanDI SexAI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI
BplI Bpu10I BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI ZraI