Restriction map for
pF1KE2368
(Linear) (Six-base) MAPSORT of: KIEE2368.seq Check: 4146 from: 1 to: 6045
KIEE2368.orf
With 182 enzymes: SgfI *
September 22, 2011 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4227 5750 6045
Size: 4227 1523 295
Fragments arranged by size:
4227 1523 295
AatII G_ACGT'C
Cuts at: 0 3839 4592 6045
Size: 3839 753 1453
Fragments arranged by size:
3839 1453 753
AccI GT'mk_AC
Cuts at: 0 3833 6045
Size: 3833 2212
Acc65I G'GTAC_C
Cuts at: 0 4220 6045
Size: 4220 1825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1433 1637 2808 3524 4023 5304 5629
Size: 727 706 204 1171 716 499 1281 325
Cuts at: 5629 6045
Size: 416
Fragments arranged by size:
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204
AfeI AGC'GCT
Cuts at: 0 857 1297 2077 3659 6045
Size: 857 440 780 1582 2386
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 551 1000 4331 5066 5291 6045
Size: 551 449 3331 735 225 754
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 1016 6045
Size: 1016 5029
AhdI GACnn_n'nnGTC
Cuts at: 0 6033 6045
Size: 6033 12
AleI CACnn'nnGTG
Cuts at: 0 4126 6045
Size: 4126 1919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5758 6045
Size: 5758 287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1728 1760 5614 5646 6045
Size: 1728 32 3854 32 399
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1456 1926 2848 3278 3298 6045
Size: 1456 470 922 430 20 2747
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 678 726 1919 1986 3889 6045
Size: 678 48 1193 67 1903 2156
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 414 3914 6045
Size: 414 3500 2131
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 622 2230 5395 6045
Size: 622 1608 3165 650
Fragments arranged by size:
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AvaI C'yCGr_G
Cuts at: 0 20 139 533 3115 5210 6045
Size: 20 119 394 2582 2095 835
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 3120 4922 6045
Size: 3120 1802 1123
Fragments arranged by size:
3120 1802 1123
BanI G'GyrC_C
Cuts at: 0 25 865 955 1019 1686 3054 3109 3982
Size: 25 840 90 64 667 1368 55 873
Cuts at: 3982 4220 5738 6045
Size: 238 1518 307
Fragments arranged by size:
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64 55 25
BanII G_rGCy'C
Cuts at: 0 224 236 678 726 1919 1986 3131 3446
Size: 224 12 442 48 1193 67 1145 315
Cuts at: 3446 3889 3936 4504 5230 5351 5669 6045
Size: 443 47 568 726 121 318 376
Fragments arranged by size:
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315 224 121 67 48 47 12
BbeI G_GCGC'C
Cuts at: 0 3986 6045
Size: 3986 2059
BbsI GAAGACnn'nnnn_
Cuts at: 0 715 765 1439 2256 2436 2745 3700 3735
Size: 715 50 674 817 180 309 955 35
Cuts at: 3735 6045
Size: 2310
Fragments arranged by size:
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35
BbvCI CC'TCA_GC
Cuts at: 0 781 1543 1987 2602 5146 6045
Size: 781 762 444 615 2544 899
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 558 1054 1088 3304 3338 5797 5831
Size: 524 34 496 34 2216 34 2459 34
Cuts at: 5831 6045
Size: 214
Fragments arranged by size:
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34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1353 6045
Size: 1353 4692
BglI GCCn_nnn'nGGC
Cuts at: 0 1914 2634 6045
Size: 1914 720 3411
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 195 1160 5384 5654 5994 6045
Size: 195 965 4224 270 340 51
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 2815 3440 6045
Size: 2815 625 2605
Fragments arranged by size:
2815 2605 625
Bme1580I G_kGCm'C
Cuts at: 0 28 418 678 726 1691 1716 1919 1986
Size: 28 390 260 48 965 25 203 67
Cuts at: 1986 2514 3112 3143 3205 3889 3918 4813 6045
Size: 528 598 31 62 684 29 895 1232
Fragments arranged by size:
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203 67 62 48 31 29 28 25
BmgBI CAC'GTC
Cuts at: 0 5123 6045
Size: 5123 922
BmrI ACTGGGnnnn_n'
Cuts at: 0 1396 1721 1819 2524 5174 6045
Size: 1396 325 98 705 2650 871
Fragments arranged by size:
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BmtI G_CTAG'C
Cuts at: 0 2539 6045
Size: 2539 3506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5851 5880 6045
Size: 5851 29 165
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 2227 2860 3300 3469 3772 4272 5332
Size: 259 1968 633 440 169 303 500 1060
Cuts at: 5332 5871 5944 6045
Size: 539 73 101
Fragments arranged by size:
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169 101 73
Bpu10I CC'TnA_GC
Cuts at: 0 781 1543 1987 2371 2602 5146 5822 6045
Size: 781 762 444 384 231 2544 676 223
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1306 3733 3748 6045
Size: 931 375 2427 15 2297
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1270 1598 1806 2695 2953 3379 6045
Size: 1270 328 208 889 258 426 2666
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 5847 6045
Size: 5847 198
BsaBI GATnn'nnATC
Cuts at: 0 2113 6045
Size: 2113 3932
BsaHI Gr'CG_yC
Cuts at: 0 3836 3983 4589 5603 6045
Size: 3836 147 606 1014 442
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 308 359 1005 1016 3244 3264 4165 6018
Size: 308 51 646 11 2228 20 901 1853
Cuts at: 6018 6045
Size: 27
Fragments arranged by size:
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11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1728 1758 3199 3229 3388 3418 4831 4861
Size: 1728 30 1441 30 159 30 1413 30
Cuts at: 4861 5614 5644 6045
Size: 753 30 401
Fragments arranged by size:
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30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 160 1264 1297 1446 1474 1843 1918
Size: 157 3 1104 33 149 28 369 75
Cuts at: 1918 1975 2044 2251 3202 3223 3226 3294 3700
Size: 57 69 207 951 21 3 68 406
Cuts at: 3700 4000 4477 5134 5350 5638 5883 5892 6045
Size: 300 477 657 216 288 245 9 153
Fragments arranged by size:
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245 216 207 157 153 149 75 69
68 57 33 28 21 9 3 3
BseYI C'CCAG_C
Cuts at: 0 161 446 994 1118 2072 2385 3004 3233
Size: 161 285 548 124 954 313 619 229
Cuts at: 3233 4497 5132 5229 6045
Size: 1264 635 97 816
Fragments arranged by size:
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229 161 124 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 2849 6045
Size: 532 2317 3196
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 5129 6045
Size: 5129 916
BsiHKAI G_wGCw'C
Cuts at: 0 224 418 1877 1974 2213 3446 3608 3918
Size: 224 194 1459 97 239 1233 162 310
Cuts at: 3918 3936 4820 5344 6045
Size: 18 884 524 701
Fragments arranged by size:
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194 162 97 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2071 3037 3843 6045
Size: 2071 966 806 2202
Fragments arranged by size:
2202 2071 966 806
Bsp1286I G_dGCh'C
Cuts at: 0 28 224 236 418 678 726 1691 1716
Size: 28 196 12 182 260 48 965 25
Cuts at: 1716 1877 1919 1974 1986 2213 2514 3112 3131
Size: 161 42 55 12 227 301 598 19
Cuts at: 3131 3143 3205 3446 3608 3889 3918 3936 4504
Size: 12 62 241 162 281 29 18 568
Cuts at: 4504 4813 4820 5230 5344 5351 5669 6045
Size: 309 7 410 114 7 318 376
Fragments arranged by size:
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281 260 241 227 196 182 162 161
114 62 55 48 42 29 28 25
19 18 12 12 12 7 7
BspEI T'CCGG_A
Cuts at: 0 359 1005 3264 6018 6045
Size: 359 646 2259 2754 27
Fragments arranged by size:
2754 2259 646 359 27
BspHI T'CATG_A
Cuts at: 0 3635 3998 4454 4946 6045
Size: 3635 363 456 492 1099
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 179 950 2238 3595 4227 4697 5522
Size: 32 147 771 1288 1357 632 470 825
Cuts at: 5522 5750 6045
Size: 228 295
Fragments arranged by size:
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147 32
BsrBI CCG'CTC
Cuts at: 0 367 3690 6045
Size: 367 3323 2355
Fragments arranged by size:
3323 2355 367
BsrDI GCAATG_nn'
Cuts at: 0 645 1244 1520 1797 2303 3011 5208 5297
Size: 645 599 276 277 506 708 2197 89
Cuts at: 5297 5541 6045
Size: 244 504
Fragments arranged by size:
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244 89
BsrFI r'CCGG_y
Cuts at: 0 1016 5128 6045
Size: 1016 4112 917
Fragments arranged by size:
4112 1016 917
BsrGI T'GTAC_A
Cuts at: 0 4334 5294 6045
Size: 4334 960 751
Fragments arranged by size:
4334 960 751
BssSI C'ACGA_G
Cuts at: 0 1333 6045
Size: 1333 4712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1627 2983 3587 5102 5543 5745 6045
Size: 1627 1356 604 1515 441 202 300
Fragments arranged by size:
1627 1515 1356 604 441 300 202
BstEII G'GTnAC_C
Cuts at: 0 4623 6045
Size: 4623 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 581 2016 2057 3150 3867 4635 5310 6045
Size: 581 1435 41 1093 717 768 675 735
Fragments arranged by size:
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BstYI r'GATC_y
Cuts at: 0 195 1160 3120 3455 4922 5384 5612 5654
Size: 195 965 1960 335 1467 462 228 42
Cuts at: 5654 5994 6045
Size: 340 51
Fragments arranged by size:
1960 1467 965 462 340 335 228 195
51 42
Bsu36I CC'TnA_GG
Cuts at: 0 826 1144 6045
Size: 826 318 4901
Fragments arranged by size:
4901 826 318
BtgI C'CryG_G
Cuts at: 0 99 574 878 1047 1229 1310 1536 1575
Size: 99 475 304 169 182 81 226 39
Cuts at: 1575 1761 4528 5234 5330 5519 5711 6045
Size: 186 2767 706 96 189 192 334
Fragments arranged by size:
2767 706 475 334 304 226 192 189
186 182 169 99 96 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1254 3129 5823 6045
Size: 1254 1875 2694 222
Fragments arranged by size:
2694 1875 1254 222
BtsI GCAGTG_nn'
Cuts at: 0 1678 3355 3657 3898 6045
Size: 1678 1677 302 241 2147
Fragments arranged by size:
2147 1678 1677 302 241
DraI TTT'AAA
Cuts at: 0 4251 6045
Size: 4251 1794
DraIII CAC_nnn'GTG
Cuts at: 0 2174 3600 6045
Size: 2174 1426 2445
Fragments arranged by size:
2445 2174 1426
DrdI GACnn_nn'nnGTC
Cuts at: 0 895 1146 1727 6045
Size: 895 251 581 4318
Fragments arranged by size:
4318 895 581 251
EaeI y'GGCC_r
Cuts at: 0 1226 1749 2453 3152 3468 3857 4676 4700
Size: 1226 523 704 699 316 389 819 24
Cuts at: 4700 5024 5126 5327 5708 6045
Size: 324 102 201 381 337
Fragments arranged by size:
1226 819 704 699 523 389 381 337
324 316 201 102 24
EagI C'GGCC_G
Cuts at: 0 5126 6045
Size: 5126 919
EarI CTCTTCn'nnn_
Cuts at: 0 400 817 1099 2015 2200 2803 3173 4295
Size: 400 417 282 916 185 603 370 1122
Cuts at: 4295 4864 4972 4993 5113 5251 5693 5782 5804
Size: 569 108 21 120 138 442 89 22
Cuts at: 5804 6045
Size: 241
Fragments arranged by size:
1122 916 603 569 442 417 400 370
282 241 185 138 120 108 89 22
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1087 1846 2419 4861 6045
Size: 1087 759 573 2442 1184
Fragments arranged by size:
2442 1184 1087 759 573
EcoICRI GAG'CTC
Cuts at: 0 222 3444 3934 6045
Size: 222 3222 490 2111
Fragments arranged by size:
3222 2111 490 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 727 1433 1637 2227 2808 2860 3300
Size: 259 468 706 204 590 581 52 440
Cuts at: 3300 3469 3524 3772 4023 4272 5304 5332 5629
Size: 169 55 248 251 249 1032 28 297
Cuts at: 5629 5871 5944 6045
Size: 242 73 101
Fragments arranged by size:
1032 706 590 581 468 440 297 259
251 249 248 242 204 169 101 73
55 52 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1596 3469 4234 5356 5733 6045
Size: 1596 1873 765 1122 377 312
Fragments arranged by size:
1873 1596 1122 765 377 312
EcoO109I rG'GnC_Cy
Cuts at: 0 243 570 675 722 723 1199 1338 1916
Size: 243 327 105 47 1 476 139 578
Cuts at: 1916 1983 2904 3258 3275 3369 3483 3552 3886
Size: 67 921 354 17 94 114 69 334
Cuts at: 3886 5577 5832 6045
Size: 1691 255 213
Fragments arranged by size:
1691 921 578 476 354 334 327 255
243 213 139 114 105 94 69 67
47 17 1
EcoRI G'AATT_C
Cuts at: 0 2230 6045
Size: 2230 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 3810 4599 4860 6045
Size: 321 3489 789 261 1185
Fragments arranged by size:
3489 1185 789 321 261
FspI TGC'GCA
Cuts at: 0 844 3580 6045
Size: 844 2736 2465
Fragments arranged by size:
2736 2465 844
HaeII r_GCGC'y
Cuts at: 0 859 1128 1299 2079 2206 3522 3661 3986
Size: 859 269 171 780 127 1316 139 325
Cuts at: 3986 6045
Size: 2059
Fragments arranged by size:
2059 1316 859 780 325 269 171 139
127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 1351 1383 1819 1851 2599 2631
Size: 516 32 803 32 436 32 748 32
Cuts at: 2631 2681 2713 3199 3231 3388 3420 5443 5475
Size: 50 32 486 32 157 32 2023 32
Cuts at: 5475 6019 6045
Size: 544 26
Fragments arranged by size:
2023 803 748 544 516 486 436 157
50 32 32 32 32 32 32 32
32 26
HincII GTy'rAC
Cuts at: 0 4197 5289 6045
Size: 4197 1092 756
Fragments arranged by size:
4197 1092 756
KasI G'GCGC_C
Cuts at: 0 3982 6045
Size: 3982 2063
KpnI G_GTAC'C
Cuts at: 0 4224 6045
Size: 4224 1821
MluI A'CGCG_T
Cuts at: 0 551 6045
Size: 551 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2232 2355 6045
Size: 2232 123 3690
Fragments arranged by size:
3690 2232 123
MscI TGG'CCA
Cuts at: 0 1751 2455 3859 4678 4702 5026 5329 6045
Size: 1751 704 1404 819 24 324 303 716
Fragments arranged by size:
1751 1404 819 716 704 324 303 24
MslI CAynn'nnrTG
Cuts at: 0 1315 2170 2545 2722 3148 3634 3958 4051
Size: 1315 855 375 177 426 486 324 93
Cuts at: 4051 4126 4453 4597 4633 5506 6045
Size: 75 327 144 36 873 539
Fragments arranged by size:
1315 873 855 539 486 426 375 327
324 177 144 93 75 36
MspA1I CmG'CkG
Cuts at: 0 101 165 1577 1651 1980 2103 2880 2961
Size: 101 64 1412 74 329 123 777 81
Cuts at: 2961 3000 3008 3439 3611 4279 4766 5798 6045
Size: 39 8 431 172 668 487 1032 247
Fragments arranged by size:
1412 1032 777 668 487 431 329 247
172 123 101 81 74 64 39 8
NaeI GCC'GGC
Cuts at: 0 5130 6045
Size: 5130 915
NarI GG'CG_CC
Cuts at: 0 3983 6045
Size: 3983 2062
NcoI C'CATG_G
Cuts at: 0 574 1310 1761 5519 5711 6045
Size: 574 736 451 3758 192 334
Fragments arranged by size:
3758 736 574 451 334 192
NdeI CA'TA_TG
Cuts at: 0 3738 6045
Size: 3738 2307
NgoMIV G'CCGG_C
Cuts at: 0 5128 6045
Size: 5128 917
NheI G'CTAG_C
Cuts at: 0 2535 6045
Size: 2535 3510
NspI r_CATG'y
Cuts at: 0 405 1004 2574 4335 5070 5100 5295 5767
Size: 405 599 1570 1761 735 30 195 472
Cuts at: 5767 6045
Size: 278
Fragments arranged by size:
1761 1570 735 599 472 405 278 195
30
PasI CC'CwG_GG
Cuts at: 0 1716 2991 3373 3556 3867 6045
Size: 1716 1275 382 183 311 2178
Fragments arranged by size:
2178 1716 1275 382 311 183
PciI A'CATG_T
Cuts at: 0 1000 4331 5066 5291 6045
Size: 1000 3331 735 225 754
Fragments arranged by size:
3331 1000 754 735 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 2462 3278 3600 3806 4766 5547 6045
Size: 2462 816 322 206 960 781 498
Fragments arranged by size:
2462 960 816 781 498 322 206
PfoI T'CCnGG_A
Cuts at: 0 1301 1731 3271 3446 5309 6045
Size: 1301 430 1540 175 1863 736
Fragments arranged by size:
1863 1540 1301 736 430 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 1728 1760 5614 5646 6045
Size: 943 32 753 32 3854 32 399
Fragments arranged by size:
3854 943 753 399 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 570 1199 3275 3483 5577 6045
Size: 243 327 629 2076 208 2094 468
Fragments arranged by size:
2094 2076 629 468 327 243 208
PshAI GACnn'nnGTC
Cuts at: 0 4652 6045
Size: 4652 1393
PspOMI G'GGCC_C
Cuts at: 0 674 722 1915 1982 3885 6045
Size: 674 48 1193 67 1903 2160
Fragments arranged by size:
2160 1903 1193 674 67 48
PspXI vC'TCGA_Gb
Cuts at: 0 533 6045
Size: 533 5512
PstI C_TGCA'G
Cuts at: 0 176 809 2249 4238 6045
Size: 176 633 1440 1989 1807
Fragments arranged by size:
1989 1807 1440 633 176
PvuII CAG'CTG
Cuts at: 0 165 2880 3000 3008 3611 4279 4766 6045
Size: 165 2715 120 8 603 668 487 1279
Fragments arranged by size:
2715 1279 668 603 487 165 120 8
SacI G_AGCT'C
Cuts at: 0 224 3446 3936 6045
Size: 224 3222 490 2109
Fragments arranged by size:
3222 2109 490 224
SacII CC_GC'GG
Cuts at: 0 102 1578 6045
Size: 102 1476 4467
Fragments arranged by size:
4467 1476 102
SanDI GG'GwC_CC
Cuts at: 0 570 6045
Size: 570 5475
SapI GCTCTTCn'nnn_
Cuts at: 0 400 817 4295 4864 4993 6045
Size: 400 417 3478 569 129 1052
Fragments arranged by size:
3478 1052 569 417 400 129
SbfI CC_TGCA'GG
Cuts at: 0 176 6045
Size: 176 5869
ScaI AGT'ACT
Cuts at: 0 3793 4477 4840 6045
Size: 3793 684 363 1205
Fragments arranged by size:
3793 1205 684 363
SexAI A'CCwGG_T
Cuts at: 0 1208 2258 6045
Size: 1208 1050 3787
Fragments arranged by size:
3787 1208 1050
SfcI C'TryA_G
Cuts at: 0 172 258 805 2245 4234 5979 6045
Size: 172 86 547 1440 1989 1745 66
Fragments arranged by size:
1989 1745 1440 547 172 86 66
SfoI GGC'GCC
Cuts at: 0 3984 6045
Size: 3984 2061
SmaI CCC'GGG
Cuts at: 0 3117 6045
Size: 3117 2928
SmlI C'TyrA_G
Cuts at: 0 533 946 1321 3712 3763 6045
Size: 533 413 375 2391 51 2282
Fragments arranged by size:
2391 2282 533 413 375 51
SphI G_CATG'C
Cuts at: 0 2574 5100 5767 6045
Size: 2574 2526 667 278
Fragments arranged by size:
2574 2526 667 278
StuI AGG'CCT
Cuts at: 0 178 2621 4582 6045
Size: 178 2443 1961 1463
Fragments arranged by size:
2443 1961 1463 178
StyI C'CwwG_G
Cuts at: 0 574 1310 1379 1761 4184 4916 5519 5711
Size: 574 736 69 382 2423 732 603 192
Cuts at: 5711 5874 6045
Size: 163 171
Fragments arranged by size:
2423 736 732 603 574 382 192 171
163 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 1294 3759 5421 6045
Size: 280 1014 2465 1662 624
Fragments arranged by size:
2465 1662 1014 624 280
TatI w'GTAC_w
Cuts at: 0 482 3791 4175 4334 4475 4838 5294 6045
Size: 482 3309 384 159 141 363 456 751
Fragments arranged by size:
3309 751 482 456 384 363 159 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3897 3929 6045
Size: 3897 32 2116
Fragments arranged by size:
3897 2116 32
Tth111I GACn'n_nGTC
Cuts at: 0 5932 6045
Size: 5932 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1353 1759 3563 4494 5019 5577 6045
Size: 876 477 406 1804 931 525 558 468
Fragments arranged by size:
1804 931 876 558 525 477 468 406
XhoI C'TCGA_G
Cuts at: 0 533 6045
Size: 533 5512
XmaI C'CCGG_G
Cuts at: 0 3115 6045
Size: 3115 2930
XmnI GAAnn'nnTTC
Cuts at: 0 2347 2797 3793 4774 4840 6045
Size: 2347 450 996 981 66 1205
Fragments arranged by size:
2347 1205 996 981 450 66
ZraI GAC'GTC
Cuts at: 0 3837 4590 6045
Size: 3837 753 1455
Fragments arranged by size:
3837 1455 753
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SacII SanDI SapI SbfI
ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AscI AseI AsiSI AvrII BaeI BclI
BfrBI BsiWI BsmI BssHII BstBI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NotI NruI
NsiI PacI PmeI PmlI PsiI PsrI PvuI RsrII
SalI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI