Restriction map for
pF1KE2374
(Linear) (Six-base) MAPSORT of: KIEE2374.seq Check: 9529 from: 1 to: 852
KIEE2374.orf
With 182 enzymes: SgfI *
September 6, 2011 14:42 ..
AccI GT'mk_AC
Cuts at: 0 779 852
Size: 779 73
AflIII A'CryG_T
Cuts at: 0 338 852
Size: 338 514
AhdI GACnn_n'nnGTC
Cuts at: 0 78 720 852
Size: 78 642 132
Fragments arranged by size:
642 132 78
AlwNI CAG_nnn'CTG
Cuts at: 0 474 515 852
Size: 474 41 337
Fragments arranged by size:
474 337 41
ApaI G_GGCC'C
Cuts at: 0 55 852
Size: 55 797
ApaLI G'TGCA_C
Cuts at: 0 367 691 852
Size: 367 324 161
Fragments arranged by size:
367 324 161
AvaI C'yCGr_G
Cuts at: 0 54 502 614 725 852
Size: 54 448 112 111 127
Fragments arranged by size:
448 127 112 111 54
BamHI G'GATC_C
Cuts at: 0 156 852
Size: 156 696
BanII G_rGCy'C
Cuts at: 0 55 615 683 852
Size: 55 560 68 169
Fragments arranged by size:
560 169 68 55
BbvCI CC'TCA_GC
Cuts at: 0 559 852
Size: 559 293
BglII A'GATC_T
Cuts at: 0 836 852
Size: 836 16
Bme1580I G_kGCm'C
Cuts at: 0 55 371 695 852
Size: 55 316 324 157
Fragments arranged by size:
324 316 157 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 852
Size: 618 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 771 852
Size: 466 305 81
Fragments arranged by size:
466 305 81
Bpu10I CC'TnA_GC
Cuts at: 0 559 852
Size: 559 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 852
Size: 559 293
BsaAI yAC'GTr
Cuts at: 0 341 687 852
Size: 341 346 165
Fragments arranged by size:
346 341 165
BsaBI GATnn'nnATC
Cuts at: 0 823 852
Size: 823 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 745 852
Size: 490 255 107
Fragments arranged by size:
490 255 107
BseYI C'CCAG_C
Cuts at: 0 45 67 396 764 852
Size: 45 22 329 368 88
Fragments arranged by size:
368 329 88 45 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 852
Size: 36 816
BsiHKAI G_wGCw'C
Cuts at: 0 371 683 695 852
Size: 371 312 12 157
Fragments arranged by size:
371 312 157 12
Bsp1286I G_dGCh'C
Cuts at: 0 55 371 615 683 695 852
Size: 55 316 244 68 12 157
Fragments arranged by size:
316 244 157 68 55 12
BspHI T'CATG_A
Cuts at: 0 821 852
Size: 821 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 725 852
Size: 725 127
BsrBI CCG'CTC
Cuts at: 0 134 462 852
Size: 134 328 390
Fragments arranged by size:
390 328 134
BsrFI r'CCGG_y
Cuts at: 0 629 852
Size: 629 223
BssSI C'ACGA_G
Cuts at: 0 322 852
Size: 322 530
BstYI r'GATC_y
Cuts at: 0 156 218 836 852
Size: 156 62 618 16
Fragments arranged by size:
618 156 62 16
BtgI C'CryG_G
Cuts at: 0 7 852
Size: 7 845
BtsI GCAGTG_nn'
Cuts at: 0 371 656 852
Size: 371 285 196
Fragments arranged by size:
371 285 196
DraIII CAC_nnn'GTG
Cuts at: 0 153 852
Size: 153 699
EarI CTCTTCn'nnn_
Cuts at: 0 751 852
Size: 751 101
EcoICRI GAG'CTC
Cuts at: 0 681 852
Size: 681 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 771 852
Size: 466 305 81
Fragments arranged by size:
466 305 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 301 852
Size: 271 30 551
Fragments arranged by size:
551 271 30
EcoO109I rG'GnC_Cy
Cuts at: 0 51 58 541 852
Size: 51 7 483 311
Fragments arranged by size:
483 311 51 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 160 192 706 738 828 852
Size: 64 32 64 32 514 32 90 24
Fragments arranged by size:
514 90 64 64 32 32 32 24
HindIII A'AGCT_T
Cuts at: 0 289 852
Size: 289 563
MslI CAynn'nnrTG
Cuts at: 0 25 299 852
Size: 25 274 553
Fragments arranged by size:
553 274 25
MspA1I CmG'CkG
Cuts at: 0 9 88 125 495 852
Size: 9 79 37 370 357
Fragments arranged by size:
370 357 79 37 9
PfoI T'CCnGG_A
Cuts at: 0 481 852
Size: 481 371
PmlI CAC'GTG
Cuts at: 0 341 852
Size: 341 511
PpuMI rG'GwC_Cy
Cuts at: 0 541 852
Size: 541 311
PspOMI G'GGCC_C
Cuts at: 0 51 852
Size: 51 801
PspXI vC'TCGA_Gb
Cuts at: 0 502 852
Size: 502 350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 433 465 852
Size: 433 32 387
Fragments arranged by size:
433 387 32
PstI C_TGCA'G
Cuts at: 0 87 722 851 852
Size: 87 635 129 1
Fragments arranged by size:
635 129 87 1
PvuII CAG'CTG
Cuts at: 0 88 125 495 852
Size: 88 37 370 357
Fragments arranged by size:
370 357 88 37
SacI G_AGCT'C
Cuts at: 0 683 852
Size: 683 169
SacII CC_GC'GG
Cuts at: 0 10 852
Size: 10 842
SanDI GG'GwC_CC
Cuts at: 0 541 852
Size: 541 311
SfcI C'TryA_G
Cuts at: 0 83 718 847 852
Size: 83 635 129 5
Fragments arranged by size:
635 129 83 5
SmaI CCC'GGG
Cuts at: 0 56 852
Size: 56 796
SmlI C'TyrA_G
Cuts at: 0 502 574 852
Size: 502 72 278
Fragments arranged by size:
502 278 72
StyI C'CwwG_G
Cuts at: 0 107 852
Size: 107 745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 294 852
Size: 294 558
XhoI C'TCGA_G
Cuts at: 0 502 852
Size: 502 350
XmaI C'CCGG_G
Cuts at: 0 54 852
Size: 54 798
Enzymes that do cut:
AccI AflIII AhdI AlwNI ApaI ApaLI AvaI BamHI
BanII BbvCI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BssSI BstYI BtgI BtsI DraIII
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MslI
MspA1I PfoI PmlI PpuMI PspOMI PspXI PsrI PstI
PvuII SacI SacII SanDI SfcI SmaI SmlI StyI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BsaI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XmnI
ZraI