Restriction map for
pF1KE2380
(Linear) (Six-base) MAPSORT of: KIEE2380.seq Check: 6876 from: 1 to: 1887
KIEE2380.orf
With 182 enzymes: SgfI *
October 25, 2011 13:28 ..
AccI GT'mk_AC
Cuts at: 0 149 1319 1887
Size: 149 1170 568
Fragments arranged by size:
1170 568 149
Acc65I G'GTAC_C
Cuts at: 0 1292 1887
Size: 1292 595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1887
Size: 1234 653
AhdI GACnn_n'nnGTC
Cuts at: 0 1233 1887
Size: 1233 654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 798 1887
Size: 31 767 1089
Fragments arranged by size:
1089 767 31
AlwNI CAG_nnn'CTG
Cuts at: 0 39 190 1603 1887
Size: 39 151 1413 284
Fragments arranged by size:
1413 284 151 39
ApoI r'AATT_y
Cuts at: 0 1117 1887
Size: 1117 770
AvaI C'yCGr_G
Cuts at: 0 118 266 1076 1173 1648 1887
Size: 118 148 810 97 475 239
Fragments arranged by size:
810 475 239 148 118 97
BanI G'GyrC_C
Cuts at: 0 1000 1177 1292 1750 1887
Size: 1000 177 115 458 137
Fragments arranged by size:
1000 458 177 137 115
BanII G_rGCy'C
Cuts at: 0 286 323 1027 1887
Size: 286 37 704 860
Fragments arranged by size:
860 704 286 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 1042 1887
Size: 1042 845
BbvCI CC'TCA_GC
Cuts at: 0 1097 1887
Size: 1097 790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 278 1887
Size: 244 34 1609
Fragments arranged by size:
1609 244 34
BclI T'GATC_A
Cuts at: 0 506 1887
Size: 506 1381
BlpI GC'TnA_GC
Cuts at: 0 285 1887
Size: 285 1602
Bme1580I G_kGCm'C
Cuts at: 0 1753 1887
Size: 1753 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 1887
Size: 145 1742
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1440 1887
Size: 1440 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 414 1659 1887
Size: 182 232 1245 228
Fragments arranged by size:
1245 232 228 182
Bpu10I CC'TnA_GC
Cuts at: 0 1097 1705 1887
Size: 1097 608 182
Fragments arranged by size:
1097 608 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 417 1887
Size: 417 1470
BsaAI yAC'GTr
Cuts at: 0 987 1887
Size: 987 900
BsaHI Gr'CG_yC
Cuts at: 0 874 974 1631 1831 1887
Size: 874 100 657 200 56
Fragments arranged by size:
874 657 200 100 56
BsaWI w'CCGG_w
Cuts at: 0 1760 1887
Size: 1760 127
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 676 1600 1840 1887
Size: 85 591 924 240 47
Fragments arranged by size:
924 591 240 85 47
BseYI C'CCAG_C
Cuts at: 0 992 1391 1395 1887
Size: 992 399 4 492
Fragments arranged by size:
992 492 399 4
BsiHKAI G_wGCw'C
Cuts at: 0 267 323 1697 1887
Size: 267 56 1374 190
Fragments arranged by size:
1374 267 190 56
BsmI GAATG_Cn'
Cuts at: 0 186 1887
Size: 186 1701
BsmBI CGTCTCn'nnnn_
Cuts at: 0 67 694 1887
Size: 67 627 1193
Fragments arranged by size:
1193 627 67
Bsp1286I G_dGCh'C
Cuts at: 0 267 286 323 1027 1697 1753 1887
Size: 267 19 37 704 670 56 134
Fragments arranged by size:
704 670 267 134 56 37 19
BspHI T'CATG_A
Cuts at: 0 929 1730 1887
Size: 929 801 157
Fragments arranged by size:
929 801 157
BsrFI r'CCGG_y
Cuts at: 0 337 1887
Size: 337 1550
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 39 190 1603 1887
Size: 36 3 151 1413 284
Fragments arranged by size:
1413 284 151 36 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 861 1455 1887
Size: 861 594 432
Fragments arranged by size:
861 594 432
BstYI r'GATC_y
Cuts at: 0 1350 1887
Size: 1350 537
BstZ17I GTA'TAC
Cuts at: 0 1320 1887
Size: 1320 567
BtgI C'CryG_G
Cuts at: 0 560 860 1019 1091 1619 1887
Size: 560 300 159 72 528 268
Fragments arranged by size:
560 528 300 268 159 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 798 879 1887
Size: 798 81 1008
Fragments arranged by size:
1008 798 81
BtsI GCAGTG_nn'
Cuts at: 0 509 1019 1887
Size: 509 510 868
Fragments arranged by size:
868 510 509
DraI TTT'AAA
Cuts at: 0 1116 1887
Size: 1116 771
DraIII CAC_nnn'GTG
Cuts at: 0 139 1887
Size: 139 1748
DrdI GACnn_nn'nnGTC
Cuts at: 0 81 1887
Size: 81 1806
EaeI y'GGCC_r
Cuts at: 0 557 1016 1328 1887
Size: 557 459 312 559
Fragments arranged by size:
559 557 459 312
EarI CTCTTCn'nnn_
Cuts at: 0 824 1887
Size: 824 1063
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 1032 1887
Size: 60 972 855
Fragments arranged by size:
972 855 60
EcoICRI GAG'CTC
Cuts at: 0 321 1887
Size: 321 1566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 414 1234 1659 1887
Size: 182 232 820 425 228
Fragments arranged by size:
820 425 232 228 182
EcoO109I rG'GnC_Cy
Cuts at: 0 187 270 389 1887
Size: 187 83 119 1498
Fragments arranged by size:
1498 187 119 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1698 1887
Size: 1698 189
HaeII r_GCGC'y
Cuts at: 0 401 1576 1887
Size: 401 1175 311
Fragments arranged by size:
1175 401 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 1816 1848 1887
Size: 1219 32 565 32 39
Fragments arranged by size:
1219 565 39 32 32
HincII GTy'rAC
Cuts at: 0 567 1887
Size: 567 1320
KpnI G_GTAC'C
Cuts at: 0 1296 1887
Size: 1296 591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1364 1887
Size: 294 1070 523
Fragments arranged by size:
1070 523 294
MscI TGG'CCA
Cuts at: 0 1330 1887
Size: 1330 557
MslI CAynn'nnrTG
Cuts at: 0 613 859 928 1714 1887
Size: 613 246 69 786 173
Fragments arranged by size:
786 613 246 173 69
MspA1I CmG'CkG
Cuts at: 0 1395 1740 1887
Size: 1395 345 147
Fragments arranged by size:
1395 345 147
NcoI C'CATG_G
Cuts at: 0 560 1091 1887
Size: 560 531 796
Fragments arranged by size:
796 560 531
PmlI CAC'GTG
Cuts at: 0 987 1887
Size: 987 900
PpuMI rG'GwC_Cy
Cuts at: 0 270 1887
Size: 270 1617
PvuII CAG'CTG
Cuts at: 0 1395 1740 1887
Size: 1395 345 147
Fragments arranged by size:
1395 345 147
SacI G_AGCT'C
Cuts at: 0 323 1887
Size: 323 1564
SanDI GG'GwC_CC
Cuts at: 0 270 1887
Size: 270 1617
ScaI AGT'ACT
Cuts at: 0 1335 1887
Size: 1335 552
SexAI A'CCwGG_T
Cuts at: 0 1313 1887
Size: 1313 574
SfcI C'TryA_G
Cuts at: 0 60 297 1887
Size: 60 237 1590
Fragments arranged by size:
1590 237 60
SspI AAT'ATT
Cuts at: 0 575 1887
Size: 575 1312
StyI C'CwwG_G
Cuts at: 0 560 1091 1887
Size: 560 531 796
Fragments arranged by size:
796 560 531
SwaI ATTT'AAAT
Cuts at: 0 1116 1887
Size: 1116 771
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 1461 1887
Size: 127 1334 426
Fragments arranged by size:
1334 426 127
TatI w'GTAC_w
Cuts at: 0 1333 1887
Size: 1333 554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 711 743 1887
Size: 711 32 1144
Fragments arranged by size:
1144 711 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1887
Size: 1011 876
Enzymes that do cut:
AccI Acc65I AcuI AhdI AlfI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BclI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI
BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NcoI PmlI PpuMI PvuII SacI SanDI ScaI
SexAI SfcI SspI StyI SwaI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BglI BglII BmgBI BmtI
BpuEI BsaBI BsaXI BsgI BsiEI BsiWI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII Bsu36I
ClaI CspCI EagI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI Tth111I XbaI XhoI
XmaI XmnI ZraI