Restriction map for
pF1KE2383
(Linear) (Six-base) MAPSORT of: KIEE2383.seq Check: 4110 from: 1 to: 1470
KIEE2383.orf
With 182 enzymes: SgfI *
October 27, 2011 13:18 ..
AccI GT'mk_AC
Cuts at: 0 125 1470
Size: 125 1345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1459 1470
Size: 1459 11
AfeI AGC'GCT
Cuts at: 0 1179 1470
Size: 1179 291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1196 1470
Size: 1196 274
AlwNI CAG_nnn'CTG
Cuts at: 0 678 774 1161 1372 1470
Size: 678 96 387 211 98
Fragments arranged by size:
678 387 211 98 96
ApoI r'AATT_y
Cuts at: 0 1112 1243 1470
Size: 1112 131 227
Fragments arranged by size:
1112 227 131
AseI AT'TA_AT
Cuts at: 0 989 1470
Size: 989 481
AvaI C'yCGr_G
Cuts at: 0 940 1470
Size: 940 530
BamHI G'GATC_C
Cuts at: 0 413 1470
Size: 413 1057
BanI G'GyrC_C
Cuts at: 0 584 1048 1078 1470
Size: 584 464 30 392
Fragments arranged by size:
584 464 392 30
BanII G_rGCy'C
Cuts at: 0 870 1470
Size: 870 600
BbeI G_GCGC'C
Cuts at: 0 588 1052 1470
Size: 588 464 418
Fragments arranged by size:
588 464 418
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1299 1470
Size: 323 976 171
Fragments arranged by size:
976 323 171
BbvCI CC'TCA_GC
Cuts at: 0 813 1470
Size: 813 657
BfrBI ATG'CAT
Cuts at: 0 264 504 1470
Size: 264 240 966
Fragments arranged by size:
966 264 240
BlpI GC'TnA_GC
Cuts at: 0 605 1470
Size: 605 865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 1470
Size: 546 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 690 983 1470
Size: 574 116 293 487
Fragments arranged by size:
574 487 293 116
Bpu10I CC'TnA_GC
Cuts at: 0 813 1470
Size: 813 657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1470
Size: 238 1232
BsaI GGTCTCn'nnnn_
Cuts at: 0 1244 1470
Size: 1244 226
BsaHI Gr'CG_yC
Cuts at: 0 585 1049 1088 1470
Size: 585 464 39 382
Fragments arranged by size:
585 464 382 39
BsaWI w'CCGG_w
Cuts at: 0 149 1470
Size: 149 1321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 956 986 1470
Size: 956 30 484
Fragments arranged by size:
956 484 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 693 1470
Size: 693 777
BseYI C'CCAG_C
Cuts at: 0 654 1470
Size: 654 816
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 220 935 1358 1453 1470
Size: 197 23 715 423 95 17
Fragments arranged by size:
715 423 197 95 23 17
BsmI GAATG_Cn'
Cuts at: 0 234 412 492 731 759 1470
Size: 234 178 80 239 28 711
Fragments arranged by size:
711 239 234 178 80 28
Bsp1286I G_dGCh'C
Cuts at: 0 870 1470
Size: 870 600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 1470
Size: 294 1176
BsrDI GCAATG_nn'
Cuts at: 0 482 1470
Size: 482 988
BsrGI T'GTAC_A
Cuts at: 0 1148 1470
Size: 1148 322
BssSI C'ACGA_G
Cuts at: 0 849 873 1470
Size: 849 24 597
Fragments arranged by size:
849 597 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 487 1381 1470
Size: 487 894 89
Fragments arranged by size:
894 487 89
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 714 1470
Size: 312 402 756
Fragments arranged by size:
756 402 312
BstYI r'GATC_y
Cuts at: 0 65 413 1470
Size: 65 348 1057
Fragments arranged by size:
1057 348 65
BtgI C'CryG_G
Cuts at: 0 518 881 1082 1470
Size: 518 363 201 388
Fragments arranged by size:
518 388 363 201
BtsI GCAGTG_nn'
Cuts at: 0 232 626 905 1470
Size: 232 394 279 565
Fragments arranged by size:
565 394 279 232
DrdI GACnn_nn'nnGTC
Cuts at: 0 1366 1470
Size: 1366 104
EaeI y'GGCC_r
Cuts at: 0 393 1470
Size: 393 1077
EarI CTCTTCn'nnn_
Cuts at: 0 537 1470
Size: 537 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 674 1470
Size: 674 796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 690 983 1459 1470
Size: 574 116 293 476 11
Fragments arranged by size:
574 476 293 116 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1096 1237 1470
Size: 691 405 141 233
Fragments arranged by size:
691 405 233 141
EcoO109I rG'GnC_Cy
Cuts at: 0 424 1470
Size: 424 1046
EcoRI G'AATT_C
Cuts at: 0 1112 1470
Size: 1112 358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 232 1470
Size: 232 1238
HaeII r_GCGC'y
Cuts at: 0 588 685 860 1052 1181 1470
Size: 588 97 175 192 129 289
Fragments arranged by size:
588 289 192 175 129 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 369 401 1470
Size: 252 32 85 32 1069
Fragments arranged by size:
1069 252 85 32 32
KasI G'GCGC_C
Cuts at: 0 584 1048 1470
Size: 584 464 422
Fragments arranged by size:
584 464 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 62 329 954 1470
Size: 39 23 267 625 516
Fragments arranged by size:
625 516 267 39 23
MscI TGG'CCA
Cuts at: 0 395 1470
Size: 395 1075
MslI CAynn'nnrTG
Cuts at: 0 49 277 337 373 1470
Size: 49 228 60 36 1097
Fragments arranged by size:
1097 228 60 49 36
MspA1I CmG'CkG
Cuts at: 0 728 799 1437 1470
Size: 728 71 638 33
Fragments arranged by size:
728 638 71 33
NarI GG'CG_CC
Cuts at: 0 585 1049 1470
Size: 585 464 421
Fragments arranged by size:
585 464 421
NcoI C'CATG_G
Cuts at: 0 518 1082 1470
Size: 518 564 388
Fragments arranged by size:
564 518 388
NsiI A_TGCA'T
Cuts at: 0 266 506 1470
Size: 266 240 964
Fragments arranged by size:
964 266 240
PasI CC'CwG_GG
Cuts at: 0 861 1470
Size: 861 609
PfoI T'CCnGG_A
Cuts at: 0 143 1470
Size: 143 1327
PpuMI rG'GwC_Cy
Cuts at: 0 424 1470
Size: 424 1046
PshAI GACnn'nnGTC
Cuts at: 0 152 1470
Size: 152 1318
PstI C_TGCA'G
Cuts at: 0 291 692 798 1169 1185 1268 1470
Size: 291 401 106 371 16 83 202
Fragments arranged by size:
401 371 291 202 106 83 16
PvuII CAG'CTG
Cuts at: 0 799 1437 1470
Size: 799 638 33
Fragments arranged by size:
799 638 33
SapI GCTCTTCn'nnn_
Cuts at: 0 537 1470
Size: 537 933
SfcI C'TryA_G
Cuts at: 0 287 688 794 1165 1181 1264 1470
Size: 287 401 106 371 16 83 206
Fragments arranged by size:
401 371 287 206 106 83 16
SfoI GGC'GCC
Cuts at: 0 586 1050 1470
Size: 586 464 420
Fragments arranged by size:
586 464 420
SmlI C'TyrA_G
Cuts at: 0 217 1470
Size: 217 1253
SspI AAT'ATT
Cuts at: 0 243 987 1470
Size: 243 744 483
Fragments arranged by size:
744 483 243
StuI AGG'CCT
Cuts at: 0 1102 1470
Size: 1102 368
StyI C'CwwG_G
Cuts at: 0 518 1082 1470
Size: 518 564 388
Fragments arranged by size:
564 518 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1111 1470
Size: 706 405 359
Fragments arranged by size:
706 405 359
TatI w'GTAC_w
Cuts at: 0 1148 1470
Size: 1148 322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1396 1470
Size: 1396 74
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BfrBI BlpI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
NsiI PasI PfoI PpuMI PshAI PstI PvuII SapI
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI
BmtI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NspI
PacI PciI PflMI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI