Restriction map for
pF1KE2391
(Linear) (Six-base) MAPSORT of: KIEE2391.seq Check: 1662 from: 1 to: 1551
KIEE2391.orf
With 182 enzymes: SgfI *
January 26, 2012 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 438 1551
Size: 438 1113
AccI GT'mk_AC
Cuts at: 0 398 520 1551
Size: 398 122 1031
Fragments arranged by size:
1031 398 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 296 626 1324 1551
Size: 283 13 330 698 227
Fragments arranged by size:
698 330 283 227 13
AfeI AGC'GCT
Cuts at: 0 1543 1551
Size: 1543 8
AflIII A'CryG_T
Cuts at: 0 1079 1551
Size: 1079 472
AleI CACnn'nnGTG
Cuts at: 0 1021 1551
Size: 1021 530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 952 1039 1551
Size: 952 87 512
Fragments arranged by size:
952 512 87
ApaI G_GGCC'C
Cuts at: 0 1119 1551
Size: 1119 432
ApoI r'AATT_y
Cuts at: 0 280 1551
Size: 280 1271
BanI G'GyrC_C
Cuts at: 0 89 762 1235 1551
Size: 89 673 473 316
Fragments arranged by size:
673 473 316 89
BanII G_rGCy'C
Cuts at: 0 125 678 1073 1119 1551
Size: 125 553 395 46 432
Fragments arranged by size:
553 432 395 125 46
BbeI G_GCGC'C
Cuts at: 0 1239 1551
Size: 1239 312
BbsI GAAGACnn'nnnn_
Cuts at: 0 792 835 861 1551
Size: 792 43 26 690
Fragments arranged by size:
792 690 43 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 1138 1551
Size: 1138 413
BglII A'GATC_T
Cuts at: 0 371 1551
Size: 371 1180
Bme1580I G_kGCm'C
Cuts at: 0 767 1119 1453 1551
Size: 767 352 334 98
Fragments arranged by size:
767 352 334 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 31 1551
Size: 31 1520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1075 1551
Size: 364 711 476
Fragments arranged by size:
711 476 364
Bpu10I CC'TnA_GC
Cuts at: 0 541 1551
Size: 541 1010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 499 1162 1551
Size: 484 15 663 389
Fragments arranged by size:
663 484 389 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 1051 1551
Size: 205 846 500
Fragments arranged by size:
846 500 205
BsaHI Gr'CG_yC
Cuts at: 0 1236 1432 1551
Size: 1236 196 119
Fragments arranged by size:
1236 196 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1504 1534 1551
Size: 175 30 1299 30 17
Fragments arranged by size:
1299 175 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 88 160 223 435 1143 1177 1384
Size: 85 3 72 63 212 708 34 207
Cuts at: 1384 1551
Size: 167
Fragments arranged by size:
708 212 207 167 85 72 63 34
3
BseYI C'CCAG_C
Cuts at: 0 56 691 1012 1089 1105 1260 1280 1496
Size: 56 635 321 77 16 155 20 216
Cuts at: 1496 1551
Size: 55
Fragments arranged by size:
635 321 216 155 77 56 55 20
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1232 1551
Size: 1060 172 319
Fragments arranged by size:
1060 319 172
BsiEI CG_ry'CG
Cuts at: 0 649 1551
Size: 649 902
BsiHKAI G_wGCw'C
Cuts at: 0 125 242 776 854 992 1073 1551
Size: 125 117 534 78 138 81 478
Fragments arranged by size:
534 478 138 125 117 81 78
BsmI GAATG_Cn'
Cuts at: 0 276 1551
Size: 276 1275
Bsp1286I G_dGCh'C
Cuts at: 0 125 242 678 767 776 854 992 1073
Size: 125 117 436 89 9 78 138 81
Cuts at: 1073 1119 1453 1551
Size: 46 334 98
Fragments arranged by size:
436 334 138 125 117 98 89 81
78 46 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 438 1551
Size: 438 1113
BsrBI CCG'CTC
Cuts at: 0 78 443 1218 1551
Size: 78 365 775 333
Fragments arranged by size:
775 365 333 78
BsrFI r'CCGG_y
Cuts at: 0 257 644 648 810 1253 1551
Size: 257 387 4 162 443 298
Fragments arranged by size:
443 387 298 257 162 4
BstEII G'GTnAC_C
Cuts at: 0 957 1551
Size: 957 594
BstXI CCAn_nnnn'nTGG
Cuts at: 0 909 969 1551
Size: 909 60 582
Fragments arranged by size:
909 582 60
BstYI r'GATC_y
Cuts at: 0 371 1460 1551
Size: 371 1089 91
Fragments arranged by size:
1089 371 91
BstZ17I GTA'TAC
Cuts at: 0 521 1551
Size: 521 1030
BtgI C'CryG_G
Cuts at: 0 131 581 908 962 1551
Size: 131 450 327 54 589
Fragments arranged by size:
589 450 327 131 54
BtsI GCAGTG_nn'
Cuts at: 0 1194 1244 1551
Size: 1194 50 307
Fragments arranged by size:
1194 307 50
EaeI y'GGCC_r
Cuts at: 0 524 646 650 839 911 1229 1551
Size: 524 122 4 189 72 318 322
Fragments arranged by size:
524 322 318 189 122 72 4
EagI C'GGCC_G
Cuts at: 0 646 1551
Size: 646 905
EarI CTCTTCn'nnn_
Cuts at: 0 745 946 1054 1297 1551
Size: 745 201 108 243 254
Fragments arranged by size:
745 254 243 201 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 715 1551
Size: 715 836
EcoICRI GAG'CTC
Cuts at: 0 123 1071 1551
Size: 123 948 480
Fragments arranged by size:
948 480 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 296 364 626 1075 1324 1551
Size: 283 13 68 262 449 249 227
Fragments arranged by size:
449 283 262 249 227 68 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 550 1551
Size: 550 1001
EcoO109I rG'GnC_Cy
Cuts at: 0 1115 1116 1512 1551
Size: 1115 1 396 39
Fragments arranged by size:
1115 396 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 1065 1551
Size: 756 309 486
Fragments arranged by size:
756 486 309
FseI GG_CCGG'CC
Cuts at: 0 652 1551
Size: 652 899
HaeII r_GCGC'y
Cuts at: 0 1239 1545 1551
Size: 1239 306 6
Fragments arranged by size:
1239 306 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1195 1227 1504 1536 1551
Size: 817 32 346 32 277 32 15
Fragments arranged by size:
817 346 277 32 32 32 15
HincII GTy'rAC
Cuts at: 0 660 792 1338 1551
Size: 660 132 546 213
Fragments arranged by size:
660 546 213 132
HpaI GTT'AAC
Cuts at: 0 1338 1551
Size: 1338 213
KasI G'GCGC_C
Cuts at: 0 1235 1551
Size: 1235 316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1551
Size: 206 1345
MscI TGG'CCA
Cuts at: 0 841 913 1231 1551
Size: 841 72 318 320
Fragments arranged by size:
841 320 318 72
MslI CAynn'nnrTG
Cuts at: 0 760 847 907 967 1021 1444 1551
Size: 760 87 60 60 54 423 107
Fragments arranged by size:
760 423 107 87 60 60 54
MspA1I CmG'CkG
Cuts at: 0 56 1312 1382 1551
Size: 56 1256 70 169
Fragments arranged by size:
1256 169 70 56
NaeI GCC'GGC
Cuts at: 0 650 1255 1551
Size: 650 605 296
Fragments arranged by size:
650 605 296
NarI GG'CG_CC
Cuts at: 0 1236 1551
Size: 1236 315
NcoI C'CATG_G
Cuts at: 0 908 962 1551
Size: 908 54 589
Fragments arranged by size:
908 589 54
NgoMIV G'CCGG_C
Cuts at: 0 648 1253 1551
Size: 648 605 298
Fragments arranged by size:
648 605 298
NspI r_CATG'y
Cuts at: 0 1083 1551
Size: 1083 468
PasI CC'CwG_GG
Cuts at: 0 1111 1140 1551
Size: 1111 29 411
Fragments arranged by size:
1111 411 29
PciI A'CATG_T
Cuts at: 0 1079 1551
Size: 1079 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 230 1551
Size: 230 1321
PfoI T'CCnGG_A
Cuts at: 0 1052 1551
Size: 1052 499
PpuMI rG'GwC_Cy
Cuts at: 0 1512 1551
Size: 1512 39
PshAI GACnn'nnGTC
Cuts at: 0 1435 1551
Size: 1435 116
PspOMI G'GGCC_C
Cuts at: 0 1115 1551
Size: 1115 436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 559 591 1551
Size: 559 32 960
Fragments arranged by size:
960 559 32
PstI C_TGCA'G
Cuts at: 0 1472 1551
Size: 1472 79
PvuII CAG'CTG
Cuts at: 0 56 1312 1382 1551
Size: 56 1256 70 169
Fragments arranged by size:
1256 169 70 56
SacI G_AGCT'C
Cuts at: 0 125 1073 1551
Size: 125 948 478
Fragments arranged by size:
948 478 125
SanDI GG'GwC_CC
Cuts at: 0 1512 1551
Size: 1512 39
SapI GCTCTTCn'nnn_
Cuts at: 0 946 1054 1551
Size: 946 108 497
Fragments arranged by size:
946 497 108
SbfI CC_TGCA'GG
Cuts at: 0 1472 1551
Size: 1472 79
ScaI AGT'ACT
Cuts at: 0 1168 1551
Size: 1168 383
SexAI A'CCwGG_T
Cuts at: 0 794 1551
Size: 794 757
SfcI C'TryA_G
Cuts at: 0 1468 1551
Size: 1468 83
SfoI GGC'GCC
Cuts at: 0 1237 1551
Size: 1237 314
SmlI C'TyrA_G
Cuts at: 0 499 514 1177 1551
Size: 499 15 663 374
Fragments arranged by size:
663 499 374 15
StyI C'CwwG_G
Cuts at: 0 560 908 962 1154 1551
Size: 560 348 54 192 397
Fragments arranged by size:
560 397 348 192 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1026 1033 1551
Size: 1026 7 518
Fragments arranged by size:
1026 518 7
TatI w'GTAC_w
Cuts at: 0 416 1166 1551
Size: 416 750 385
Fragments arranged by size:
750 416 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 1551
Size: 307 32 1212
Fragments arranged by size:
1212 307 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 660 678 1504 1551
Size: 660 18 826 47
Fragments arranged by size:
826 660 47 18
XmnI GAAnn'nnTTC
Cuts at: 0 1048 1551
Size: 1048 503
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI ApaI
ApoI BanI BanII BbeI BbsI BciVI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BstEII BstXI BstYI BstZ17I BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I HincII HpaI KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PshAI PspOMI PsrI PstI
PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SmlI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI AlwNI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FspI
FspAI HindIII KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI
PvuI RsrII SacII SalI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI