Restriction map for
pF1KE2392
(Linear) (Six-base) MAPSORT of: KIEE2392.seq Check: 5509 from: 1 to: 1551
KIEE2392.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1312 1551
Size: 184 1128 239
Fragments arranged by size:
1128 239 184
AfeI AGC'GCT
Cuts at: 0 1235 1551
Size: 1235 316
AhdI GACnn_n'nnGTC
Cuts at: 0 1110 1551
Size: 1110 441
AleI CACnn'nnGTG
Cuts at: 0 316 1551
Size: 316 1235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1181 1213 1551
Size: 1181 32 338
Fragments arranged by size:
1181 338 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1551
Size: 1062 489
ApaI G_GGCC'C
Cuts at: 0 60 1551
Size: 60 1491
AvaI C'yCGr_G
Cuts at: 0 1019 1134 1551
Size: 1019 115 417
Fragments arranged by size:
1019 417 115
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 393 426 1551
Size: 393 33 1125
Fragments arranged by size:
1125 393 33
BamHI G'GATC_C
Cuts at: 0 267 1551
Size: 267 1284
BanI G'GyrC_C
Cuts at: 0 303 660 882 903 1355 1497 1551
Size: 303 357 222 21 452 142 54
Fragments arranged by size:
452 357 303 222 142 54 21
BanII G_rGCy'C
Cuts at: 0 60 1551
Size: 60 1491
BbeI G_GCGC'C
Cuts at: 0 664 1551
Size: 664 887
BbsI GAAGACnn'nnnn_
Cuts at: 0 354 849 1551
Size: 354 495 702
Fragments arranged by size:
702 495 354
BbvCI CC'TCA_GC
Cuts at: 0 298 1063 1551
Size: 298 765 488
Fragments arranged by size:
765 488 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 372 406 1551
Size: 372 34 1145
Fragments arranged by size:
1145 372 34
BfrBI ATG'CAT
Cuts at: 0 3 1551
Size: 3 1548
BglI GCCn_nnn'nGGC
Cuts at: 0 1315 1551
Size: 1315 236
BlpI GC'TnA_GC
Cuts at: 0 1270 1551
Size: 1270 281
Bme1580I G_kGCm'C
Cuts at: 0 60 1353 1551
Size: 60 1293 198
Fragments arranged by size:
1293 198 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 1104 1163 1551
Size: 1104 59 388
Fragments arranged by size:
1104 388 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1258 1538 1551
Size: 361 897 280 13
Fragments arranged by size:
897 361 280 13
Bpu10I CC'TnA_GC
Cuts at: 0 298 1063 1551
Size: 298 765 488
Fragments arranged by size:
765 488 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 1001 1551
Size: 1001 550
BsaHI Gr'CG_yC
Cuts at: 0 661 1551
Size: 661 890
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 994 1024 1551
Size: 172 30 792 30 527
Fragments arranged by size:
792 527 172 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 91 756 1122 1174 1183 1402 1551
Size: 16 75 665 366 52 9 219 149
Fragments arranged by size:
665 366 219 149 75 52 16 9
BseYI C'CCAG_C
Cuts at: 0 263 654 805 1055 1305 1551
Size: 263 391 151 250 250 246
Fragments arranged by size:
391 263 250 250 246 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1551
Size: 1120 431
BsiEI CG_ry'CG
Cuts at: 0 328 1551
Size: 328 1223
BsiHKAI G_wGCw'C
Cuts at: 0 392 761 842 980 1551
Size: 392 369 81 138 571
Fragments arranged by size:
571 392 369 138 81
BsmI GAATG_Cn'
Cuts at: 0 969 1551
Size: 969 582
Bsp1286I G_dGCh'C
Cuts at: 0 60 392 761 842 980 1353 1551
Size: 60 332 369 81 138 373 198
Fragments arranged by size:
373 369 332 198 138 81 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 710 1544 1551
Size: 128 582 834 7
Fragments arranged by size:
834 582 128 7
BsrDI GCAATG_nn'
Cuts at: 0 390 1340 1551
Size: 390 950 211
Fragments arranged by size:
950 390 211
BsrFI r'CCGG_y
Cuts at: 0 1500 1551
Size: 1500 51
BssSI C'ACGA_G
Cuts at: 0 391 1551
Size: 391 1160
BstEII G'GTnAC_C
Cuts at: 0 942 1551
Size: 942 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1282 1551
Size: 1282 269
BstYI r'GATC_y
Cuts at: 0 267 1521 1551
Size: 267 1254 30
Fragments arranged by size:
1254 267 30
Bsu36I CC'TnA_GG
Cuts at: 0 590 1042 1551
Size: 590 452 509
Fragments arranged by size:
590 509 452
BtgI C'CryG_G
Cuts at: 0 128 314 1013 1551
Size: 128 186 699 538
Fragments arranged by size:
699 538 186 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 1551
Size: 729 822
BtsI GCAGTG_nn'
Cuts at: 0 247 1191 1210 1551
Size: 247 944 19 341
Fragments arranged by size:
944 341 247 19
EaeI y'GGCC_r
Cuts at: 0 325 558 872 1433 1551
Size: 325 233 314 561 118
Fragments arranged by size:
561 325 314 233 118
EagI C'GGCC_G
Cuts at: 0 325 1551
Size: 325 1226
EarI CTCTTCn'nnn_
Cuts at: 0 730 868 1201 1453 1551
Size: 730 138 333 252 98
Fragments arranged by size:
730 333 252 138 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 432 1551
Size: 432 1119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 361 1258 1312 1538 1551
Size: 184 177 897 54 226 13
Fragments arranged by size:
897 226 184 177 54 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1551
Size: 1294 257
EcoO109I rG'GnC_Cy
Cuts at: 0 56 1551
Size: 56 1495
EcoRV GAT'ATC
Cuts at: 0 333 1551
Size: 333 1218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 741 1551
Size: 741 810
HaeII r_GCGC'y
Cuts at: 0 84 664 1237 1551
Size: 84 580 573 314
Fragments arranged by size:
580 573 314 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1130 1162 1551
Size: 1096 32 2 32 389
Fragments arranged by size:
1096 389 32 32 2
KasI G'GCGC_C
Cuts at: 0 660 1551
Size: 660 891
MscI TGG'CCA
Cuts at: 0 560 874 1435 1551
Size: 560 314 561 116
Fragments arranged by size:
561 560 314 116
MslI CAynn'nnrTG
Cuts at: 0 316 835 958 1551
Size: 316 519 123 593
Fragments arranged by size:
593 519 316 123
MspA1I CmG'CkG
Cuts at: 0 21 130 543 658 1059 1083 1096 1212
Size: 21 109 413 115 401 24 13 116
Cuts at: 1212 1465 1551
Size: 253 86
Fragments arranged by size:
413 401 253 116 115 109 86 24
21 13
NaeI GCC'GGC
Cuts at: 0 1502 1551
Size: 1502 49
NarI GG'CG_CC
Cuts at: 0 661 1551
Size: 661 890
NcoI C'CATG_G
Cuts at: 0 314 1013 1551
Size: 314 699 538
Fragments arranged by size:
699 538 314
NgoMIV G'CCGG_C
Cuts at: 0 1500 1551
Size: 1500 51
NsiI A_TGCA'T
Cuts at: 0 5 1551
Size: 5 1546
PasI CC'CwG_GG
Cuts at: 0 60 1551
Size: 60 1491
PflMI CCAn_nnn'nTGG
Cuts at: 0 227 320 572 690 1516 1551
Size: 227 93 252 118 826 35
Fragments arranged by size:
826 252 227 118 93 35
PfoI T'CCnGG_A
Cuts at: 0 647 1551
Size: 647 904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1378 1410 1551
Size: 1378 32 141
Fragments arranged by size:
1378 141 32
PspOMI G'GGCC_C
Cuts at: 0 56 1551
Size: 56 1495
PstI C_TGCA'G
Cuts at: 0 542 1551
Size: 542 1009
PvuII CAG'CTG
Cuts at: 0 543 1059 1096 1465 1551
Size: 543 516 37 369 86
Fragments arranged by size:
543 516 369 86 37
SacII CC_GC'GG
Cuts at: 0 131 1551
Size: 131 1420
SapI GCTCTTCn'nnn_
Cuts at: 0 1453 1551
Size: 1453 98
SfcI C'TryA_G
Cuts at: 0 538 1120 1551
Size: 538 582 431
Fragments arranged by size:
582 538 431
SfoI GGC'GCC
Cuts at: 0 662 1551
Size: 662 889
StuI AGG'CCT
Cuts at: 0 287 595 1318 1551
Size: 287 308 723 233
Fragments arranged by size:
723 308 287 233
StyI C'CwwG_G
Cuts at: 0 314 800 1013 1551
Size: 314 486 213 538
Fragments arranged by size:
538 486 314 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 1123 1551
Size: 282 841 428
Fragments arranged by size:
841 428 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1551
Size: 994 32 525
Fragments arranged by size:
994 525 32
Tth111I GACn'n_nGTC
Cuts at: 0 358 1420 1551
Size: 358 1062 131
Fragments arranged by size:
1062 358 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 1551
Size: 645 906
Enzymes that do cut:
AcuI AfeI AhdI AleI AloI AlwNI ApaI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
PasI PflMI PfoI PpiI PspOMI PstI PvuII SacII
SapI SfcI SfoI StuI StyI TaqII TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BciVI
BclI BglII BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NspI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmaI XmnI ZraI