Restriction map for
pF1KE2399
(Linear) (Six-base) MAPSORT of: KIEE2399.seq Check: 9308 from: 1 to: 6855
KIEE2399.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2072 4887 6855
Size: 2072 2815 1968
Fragments arranged by size:
2815 2072 1968
AccI GT'mk_AC
Cuts at: 0 1615 4280 5746 6855
Size: 1615 2665 1466 1109
Fragments arranged by size:
2665 1615 1466 1109
Acc65I G'GTAC_C
Cuts at: 0 1247 1286 6855
Size: 1247 39 5569
Fragments arranged by size:
5569 1247 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 319 1879 5375 6269 6311 6405 6855
Size: 91 228 1560 3496 894 42 94 450
Fragments arranged by size:
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AfeI AGC'GCT
Cuts at: 0 548 808 1786 5938 6855
Size: 548 260 978 4152 917
Fragments arranged by size:
4152 978 917 548 260
AflIII A'CryG_T
Cuts at: 0 5738 6855
Size: 5738 1117
AhdI GACnn_n'nnGTC
Cuts at: 0 5742 6855
Size: 5742 1113
AleI CACnn'nnGTG
Cuts at: 0 6451 6855
Size: 6451 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2185 2726 4268 6259 6469 6855
Size: 2185 541 1542 1991 210 386
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2766 2798 6855
Size: 2766 32 4057
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 204 3361 4861 6348 6855
Size: 204 3157 1500 1487 507
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 181 281 325 593 959 985 1059 3431
Size: 181 100 44 268 366 26 74 2372
Cuts at: 3431 3754 3912 4561 5690 6322 6855
Size: 323 158 649 1129 632 533
Fragments arranged by size:
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181 158 100 74 44 26
ApoI r'AATT_y
Cuts at: 0 3606 6855
Size: 3606 3249
AseI AT'TA_AT
Cuts at: 0 2801 6855
Size: 2801 4054
AvaI C'yCGr_G
Cuts at: 0 699 914 934 1047 1090 3945 6201 6229
Size: 699 215 20 113 43 2855 2256 28
Cuts at: 6229 6855
Size: 626
Fragments arranged by size:
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20
AvrII C'CTAG_G
Cuts at: 0 325 6855
Size: 325 6530
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1231 1264 1480 1513 6855
Size: 1231 33 216 33 5342
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 3557 3690 5235 6855
Size: 3557 133 1545 1620
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 399 970 1247 1286 2506 4039 4405 4483
Size: 399 571 277 39 1220 1533 366 78
Cuts at: 4483 6078 6855
Size: 1595 777
Fragments arranged by size:
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78 39
BanII G_rGCy'C
Cuts at: 0 181 281 325 472 581 593 697 721
Size: 181 100 44 147 109 12 104 24
Cuts at: 721 959 985 1059 1090 1968 2359 3431 3754
Size: 238 26 74 31 878 391 1072 323
Cuts at: 3754 3912 4546 4561 5144 5690 6322 6855
Size: 158 634 15 583 546 632 533
Fragments arranged by size:
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323 238 181 158 147 109 104 100
74 44 31 26 24 15 12
BbeI G_GCGC'C
Cuts at: 0 403 974 6855
Size: 403 571 5881
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 495 779 3338 6855
Size: 495 284 2559 3517
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 1133 1175 1433 1694 1739 1839 4199 4268
Size: 1133 42 258 261 45 100 2360 69
Cuts at: 4268 4466 6364 6855
Size: 198 1898 491
Fragments arranged by size:
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69 45 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1281 1315 3909 3943 6855
Size: 1281 34 2594 34 2912
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 1745 4764 6855
Size: 1745 3019 2091
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 2819 5414 6855
Size: 2819 2595 1441
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 2211 2523 2703 6855
Size: 2211 312 180 4152
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 493 2178 3758 5604 6520 6855
Size: 493 1685 1580 1846 916 335
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 5177 6855
Size: 5177 1678
BlpI GC'TnA_GC
Cuts at: 0 691 800 2304 4475 6855
Size: 691 109 1504 2171 2380
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 181 281 325 593 959 985 1059 3250
Size: 181 100 44 268 366 26 74 2191
Cuts at: 3250 3431 3754 3912 4561 4685 5690 6322 6855
Size: 181 323 158 649 124 1005 632 533
Fragments arranged by size:
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181 181 158 124 100 74 44 26
BmgBI CAC'GTC
Cuts at: 0 899 6855
Size: 899 5956
BmrI ACTGGGnnnn_n'
Cuts at: 0 1740 2180 2274 2465 3435 3632 3914 4193
Size: 1740 440 94 191 970 197 282 279
Cuts at: 4193 4849 6279 6855
Size: 656 1430 576
Fragments arranged by size:
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197 191 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3731 6759 6855
Size: 3731 3028 96
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 747 1516 1842 1893 1997 2507 2546
Size: 595 152 769 326 51 104 510 39
Cuts at: 2546 2912 3476 3701 5690 6451 6855
Size: 366 564 225 1989 761 404
Fragments arranged by size:
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326 225 152 104 51 39
Bpu10I CC'TnA_GC
Cuts at: 0 1133 1175 1433 1694 1739 1839 1888 2130
Size: 1133 42 258 261 45 100 49 242
Cuts at: 2130 4199 4268 4466 6364 6524 6855
Size: 2069 69 198 1898 160 331
Fragments arranged by size:
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160 100 69 49 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1796 5011 6855
Size: 1796 3215 1844
Fragments arranged by size:
3215 1844 1796
BsaI GGTCTCn'nnnn_
Cuts at: 0 1164 1832 4376 5041 5884 6855
Size: 1164 668 2544 665 843 971
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 3414 5176 6855
Size: 3414 1762 1679
Fragments arranged by size:
3414 1762 1679
BsaHI Gr'CG_yC
Cuts at: 0 400 971 6855
Size: 400 571 5884
Fragments arranged by size:
5884 571 400
BsaWI w'CCGG_w
Cuts at: 0 2329 2672 5846 6855
Size: 2329 343 3174 1009
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1514 1544 2373 2403 2544 2559 2574 2589
Size: 1514 30 829 30 141 15 15 15
Cuts at: 2589 2766 2796 6130 6160 6424 6454 6855
Size: 177 30 3334 30 264 30 401
Fragments arranged by size:
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30 30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 395 750 768 2036 3109 3485 4980
Size: 112 283 355 18 1268 1073 376 1495
Cuts at: 4980 5371 5419 6115 6634 6855
Size: 391 48 696 519 221
Fragments arranged by size:
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283 221 112 48 18
BseYI C'CCAG_C
Cuts at: 0 204 435 593 860 904 1375 1457 1556
Size: 204 231 158 267 44 471 82 99
Cuts at: 1556 1568 1607 1721 1760 1778 2294 3039 3377
Size: 12 39 114 39 18 516 745 338
Cuts at: 3377 4063 4218 4223 4229 4256 4415 5832 6855
Size: 686 155 5 6 27 159 1417 1023
Fragments arranged by size:
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231 204 159 158 155 114 99 82
44 39 39 27 18 12 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2495 4379 4582 4804 4909 5509 5521 6034
Size: 2495 1884 203 222 105 600 12 513
Cuts at: 6034 6580 6855
Size: 546 275
Fragments arranged by size:
2495 1884 600 546 513 275 222 203
105 12
BsiEI CG_ry'CG
Cuts at: 0 134 422 465 497 845 1043 6855
Size: 134 288 43 32 348 198 5812
Fragments arranged by size:
5812 348 288 198 134 43 32
BsiHKAI G_wGCw'C
Cuts at: 0 697 1968 1987 3904 4540 5053 5144 6855
Size: 697 1271 19 1917 636 513 91 1711
Fragments arranged by size:
1917 1711 1271 697 636 513 91 19
BsmI GAATG_Cn'
Cuts at: 0 3316 6855
Size: 3316 3539
Bsp1286I G_dGCh'C
Cuts at: 0 181 281 325 472 581 593 697 721
Size: 181 100 44 147 109 12 104 24
Cuts at: 721 959 985 1059 1090 1968 1987 2359 3250
Size: 238 26 74 31 878 19 372 891
Cuts at: 3250 3431 3754 3904 3912 4540 4546 4561 4685
Size: 181 323 150 8 628 6 15 124
Cuts at: 4685 5053 5144 5690 6322 6855
Size: 368 91 546 632 533
Fragments arranged by size:
891 878 632 628 546 533 372 368
323 238 181 181 150 147 124 109
104 100 91 74 44 31 26 24
19 15 12 8 6
BspEI T'CCGG_A
Cuts at: 0 2329 6855
Size: 2329 4526
BspHI T'CATG_A
Cuts at: 0 5102 6855
Size: 5102 1753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2072 4837 4869 4887 6855
Size: 2072 2765 32 18 1968
Fragments arranged by size:
2765 2072 1968 32 18
BsrBI CCG'CTC
Cuts at: 0 557 1119 6855
Size: 557 562 5736
Fragments arranged by size:
5736 562 557
BsrDI GCAATG_nn'
Cuts at: 0 243 2234 3523 4302 5994 6551 6855
Size: 243 1991 1289 779 1692 557 304
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 262 739 844 973 2594 3790 6855
Size: 262 477 105 129 1621 1196 3065
Fragments arranged by size:
3065 1621 1196 477 262 129 105
BsrGI T'GTAC_A
Cuts at: 0 4163 6855
Size: 4163 2692
BssHII G'CGCG_C
Cuts at: 0 6707 6855
Size: 6707 148
BssSI C'ACGA_G
Cuts at: 0 4629 6545 6855
Size: 4629 1916 310
Fragments arranged by size:
4629 1916 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4212 4425 6547 6855
Size: 4212 213 2122 308
Fragments arranged by size:
4212 2122 308 213
BstBI TT'CG_AA
Cuts at: 0 5964 6855
Size: 5964 891
BstEII G'GTnAC_C
Cuts at: 0 3769 5209 6855
Size: 3769 1440 1646
Fragments arranged by size:
3769 1646 1440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 517 1149 6855
Size: 36 481 632 5706
Fragments arranged by size:
5706 632 481 36
BstYI r'GATC_y
Cuts at: 0 3386 3415 3515 3557 3690 5177 5235 5858
Size: 3386 29 100 42 133 1487 58 623
Cuts at: 5858 6855
Size: 997
Fragments arranged by size:
3386 1487 997 623 133 100 58 42
29
BstZ17I GTA'TAC
Cuts at: 0 4281 6855
Size: 4281 2574
Bsu36I CC'TnA_GG
Cuts at: 0 2630 2828 3741 3887 4748 6855
Size: 2630 198 913 146 861 2107
Fragments arranged by size:
2630 2107 913 861 198 146
BtgI C'CryG_G
Cuts at: 0 185 416 491 612 818 839 1322 1949
Size: 185 231 75 121 206 21 483 627
Cuts at: 1949 2375 2501 2585 2846 2858 3104 3458 4641
Size: 426 126 84 261 12 246 354 1183
Cuts at: 4641 5273 6855
Size: 632 1582
Fragments arranged by size:
1582 1183 632 627 483 426 354 261
246 231 206 185 126 121 84 75
21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 401 3829 6855
Size: 401 3428 3026
Fragments arranged by size:
3428 3026 401
BtsI GCAGTG_nn'
Cuts at: 0 1009 2159 3264 4310 6563 6855
Size: 1009 1150 1105 1046 2253 292
Fragments arranged by size:
2253 1150 1105 1046 1009 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 225 260 6662 6697 6855
Size: 225 35 6402 35 158
Fragments arranged by size:
6402 225 158 35 35
DraI TTT'AAA
Cuts at: 0 5192 6855
Size: 5192 1663
DraIII CAC_nnn'GTG
Cuts at: 0 2188 2419 4490 6855
Size: 2188 231 2071 2365
Fragments arranged by size:
2365 2188 2071 231
DrdI GACnn_nn'nnGTC
Cuts at: 0 3441 6855
Size: 3441 3414
EaeI y'GGCC_r
Cuts at: 0 2 131 419 462 494 842 1040 2179
Size: 2 129 288 43 32 348 198 1139
Cuts at: 2179 2190 2615 2861 4111 4377 4777 6407 6512
Size: 11 425 246 1250 266 400 1630 105
Cuts at: 6512 6722 6846 6855
Size: 210 124 9
Fragments arranged by size:
1630 1250 1139 425 400 348 288 266
246 210 198 129 124 105 43 32
11 9 2
EagI C'GGCC_G
Cuts at: 0 131 419 462 494 842 1040 6855
Size: 131 288 43 32 348 198 5815
Fragments arranged by size:
5815 348 288 198 131 43 32
EarI CTCTTCn'nnn_
Cuts at: 0 5315 5327 6855
Size: 5315 12 1528
Fragments arranged by size:
5315 1528 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 272 536 762 1003 5547 6855
Size: 272 264 226 241 4544 1308
Fragments arranged by size:
4544 1308 272 264 241 226
EcoICRI GAG'CTC
Cuts at: 0 695 1966 5142 6855
Size: 695 1271 3176 1713
Fragments arranged by size:
3176 1713 1271 695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 319 595 747 1516 1842 1879 1893
Size: 91 228 276 152 769 326 37 14
Cuts at: 1893 1997 2507 2546 2912 3476 3701 5375 5690
Size: 104 510 39 366 564 225 1674 315
Cuts at: 5690 6269 6311 6405 6451 6855
Size: 579 42 94 46 404
Fragments arranged by size:
1674 769 579 564 510 404 366 326
315 276 228 225 152 104 94 91
46 42 39 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1223 2330 3800 3878 6523 6855
Size: 1223 1107 1470 78 2645 332
Fragments arranged by size:
2645 1470 1223 1107 332 78
EcoO109I rG'GnC_Cy
Cuts at: 0 177 178 322 570 589 590 956 981
Size: 177 1 144 248 19 1 366 25
Cuts at: 981 1055 1216 1268 1372 2080 2833 3427 3428
Size: 74 161 52 104 708 753 594 1
Cuts at: 3428 3750 3751 3847 3883 3908 3909 4557 4558
Size: 322 1 96 36 25 1 648 1
Cuts at: 4558 4697 4849 5308 5686 5687 6300 6319 6855
Size: 139 152 459 378 1 613 19 536
Fragments arranged by size:
753 708 648 613 594 536 459 378
366 322 248 177 161 152 144 139
104 96 74 52 36 25 25 19
19 1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3606 6855
Size: 3606 3249
EcoRV GAT'ATC
Cuts at: 0 4914 6855
Size: 4914 1941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5317 6855
Size: 5317 1538
HaeII r_GCGC'y
Cuts at: 0 403 550 662 770 810 974 1788 4335
Size: 403 147 112 108 40 164 814 2547
Cuts at: 4335 5940 6855
Size: 1605 915
Fragments arranged by size:
2547 1605 915 814 403 164 147 112
108 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1158 1190 2160 2192 2373 2405
Size: 982 32 144 32 970 32 181 32
Cuts at: 2405 2559 2591 2811 2843 3378 3410 3942 3974
Size: 154 32 220 32 535 32 532 32
Cuts at: 3974 4373 4375 4405 4407 5728 5760 6855
Size: 399 2 30 2 1321 32 1095
Fragments arranged by size:
1321 1095 982 970 535 532 399 220
181 154 144 32 32 32 32 32
32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 0 1390 1616 2924 3201 3446 6855
Size: 1390 226 1308 277 245 3409
Fragments arranged by size:
3409 1390 1308 277 245 226
HindIII A'AGCT_T
Cuts at: 0 5431 5488 6855
Size: 5431 57 1367
Fragments arranged by size:
5431 1367 57
HpaI GTT'AAC
Cuts at: 0 2924 6855
Size: 2924 3931
KasI G'GCGC_C
Cuts at: 0 399 970 6855
Size: 399 571 5885
Fragments arranged by size:
5885 571 399
KpnI G_GTAC'C
Cuts at: 0 1251 1290 6855
Size: 1251 39 5565
Fragments arranged by size:
5565 1251 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2177 3959 5184 6191 6755 6855
Size: 2177 1782 1225 1007 564 100
Fragments arranged by size:
2177 1782 1225 1007 564 100
MscI TGG'CCA
Cuts at: 0 2192 2617 2863 6409 6514 6724 6848 6855
Size: 2192 425 246 3546 105 210 124 7
Fragments arranged by size:
3546 2192 425 246 210 124 105 7
MslI CAynn'nnrTG
Cuts at: 0 2350 2938 3109 6451 6556 6855
Size: 2350 588 171 3342 105 299
Fragments arranged by size:
3342 2350 588 299 171 105
MspA1I CmG'CkG
Cuts at: 0 93 130 204 269 418 493 841 1030
Size: 93 37 74 65 149 75 348 189
Cuts at: 1030 1100 1284 1379 3358 4321 4465 6289 6855
Size: 70 184 95 1979 963 144 1824 566
Fragments arranged by size:
1979 1824 963 566 348 189 184 149
144 95 93 75 74 70 65 37
NaeI GCC'GGC
Cuts at: 0 264 741 846 975 3792 6855
Size: 264 477 105 129 2817 3063
Fragments arranged by size:
3063 2817 477 264 129 105
NarI GG'CG_CC
Cuts at: 0 400 971 6855
Size: 400 571 5884
Fragments arranged by size:
5884 571 400
NcoI C'CATG_G
Cuts at: 0 818 1322 1949 2375 2501 2585 2846 2858
Size: 818 504 627 426 126 84 261 12
Cuts at: 2858 3104 3458 4641 5273 6855
Size: 246 354 1183 632 1582
Fragments arranged by size:
1582 1183 818 632 627 504 426 354
261 246 126 84 12
NdeI CA'TA_TG
Cuts at: 0 1182 4128 4662 6855
Size: 1182 2946 534 2193
Fragments arranged by size:
2946 2193 1182 534
NgoMIV G'CCGG_C
Cuts at: 0 262 739 844 973 3790 6855
Size: 262 477 105 129 2817 3065
Fragments arranged by size:
3065 2817 477 262 129 105
NotI GC'GGCC_GC
Cuts at: 0 419 494 1040 6855
Size: 419 75 546 5815
Fragments arranged by size:
5815 546 419 75
NsiI A_TGCA'T
Cuts at: 0 2213 2525 2705 6855
Size: 2213 312 180 4150
Fragments arranged by size:
4150 2213 312 180
NspI r_CATG'y
Cuts at: 0 2707 3672 4428 4734 5644 5742 6654 6855
Size: 2707 965 756 306 910 98 912 201
Fragments arranged by size:
2707 965 912 910 756 306 201 98
PasI CC'CwG_GG
Cuts at: 0 1252 2394 3625 3796 4554 5128 6013 6315
Size: 1252 1142 1231 171 758 574 885 302
Cuts at: 6315 6855
Size: 540
Fragments arranged by size:
1252 1231 1142 885 758 574 540 302
171
PciI A'CATG_T
Cuts at: 0 5738 6855
Size: 5738 1117
PflMI CCAn_nnn'nTGG
Cuts at: 0 2188 2419 2548 3625 4120 5698 6855
Size: 2188 231 129 1077 495 1578 1157
Fragments arranged by size:
2188 1578 1157 1077 495 231 129
PfoI T'CCnGG_A
Cuts at: 0 1974 3560 6167 6251 6380 6855
Size: 1974 1586 2607 84 129 475
Fragments arranged by size:
2607 1974 1586 475 129 84
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2766 2798 4780 4812 6855
Size: 2766 32 1982 32 2043
Fragments arranged by size:
2766 2043 1982 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1216 1268 2833 3847 4849 5308 6300 6855
Size: 1216 52 1565 1014 1002 459 992 555
Fragments arranged by size:
1565 1216 1014 1002 992 555 459 52
PshAI GACnn'nnGTC
Cuts at: 0 1221 3196 6855
Size: 1221 1975 3659
Fragments arranged by size:
3659 1975 1221
PspOMI G'GGCC_C
Cuts at: 0 177 277 321 589 955 981 1055 3427
Size: 177 100 44 268 366 26 74 2372
Cuts at: 3427 3750 3908 4557 5686 6318 6855
Size: 323 158 649 1129 632 537
Fragments arranged by size:
2372 1129 649 632 537 366 323 268
177 158 100 74 44 26
PstI C_TGCA'G
Cuts at: 0 221 554 2154 2877 6855
Size: 221 333 1600 723 3978
Fragments arranged by size:
3978 1600 723 333 221
PvuII CAG'CTG
Cuts at: 0 1030 3358 4321 4465 6289 6855
Size: 1030 2328 963 144 1824 566
Fragments arranged by size:
2328 1824 1030 963 566 144
SacI G_AGCT'C
Cuts at: 0 697 1968 5144 6855
Size: 697 1271 3176 1711
Fragments arranged by size:
3176 1711 1271 697
SacII CC_GC'GG
Cuts at: 0 419 494 842 6855
Size: 419 75 348 6013
Fragments arranged by size:
6013 419 348 75
SalI G'TCGA_C
Cuts at: 0 1614 6855
Size: 1614 5241
SanDI GG'GwC_CC
Cuts at: 0 1268 2833 6855
Size: 1268 1565 4022
Fragments arranged by size:
4022 1565 1268
SfcI C'TryA_G
Cuts at: 0 217 550 945 2150 2873 3777 6855
Size: 217 333 395 1205 723 904 3078
Fragments arranged by size:
3078 1205 904 723 395 333 217
SfoI GGC'GCC
Cuts at: 0 401 972 6855
Size: 401 571 5883
Fragments arranged by size:
5883 571 401
SmaI CCC'GGG
Cuts at: 0 916 936 1092 6855
Size: 916 20 156 5763
Fragments arranged by size:
5763 916 156 20
SmlI C'TyrA_G
Cuts at: 0 1811 5026 6855
Size: 1811 3215 1829
Fragments arranged by size:
3215 1829 1811
SpeI A'CTAG_T
Cuts at: 0 6676 6855
Size: 6676 179
SphI G_CATG'C
Cuts at: 0 4734 6855
Size: 4734 2121
SspI AAT'ATT
Cuts at: 0 5155 6855
Size: 5155 1700
StyI C'CwwG_G
Cuts at: 0 325 818 1322 1949 2007 2375 2501 2585
Size: 325 493 504 627 58 368 126 84
Cuts at: 2585 2846 2858 2907 3104 3458 4035 4641 5273
Size: 261 12 49 197 354 577 606 632
Cuts at: 5273 6114 6179 6719 6725 6855
Size: 841 65 540 6 130
Fragments arranged by size:
841 632 627 606 577 540 504 493
368 354 325 261 197 130 126 84
65 58 49 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2199 2494 2839 2979 3031 3057 3757 4048
Size: 2199 295 345 140 52 26 700 291
Cuts at: 4048 5197 5741 5782 6026 6077 6485 6855
Size: 1149 544 41 244 51 408 370
Fragments arranged by size:
2199 1149 700 544 408 370 345 295
291 244 140 52 51 41 26
TatI w'GTAC_w
Cuts at: 0 632 2282 4163 6831 6855
Size: 632 1650 1881 2668 24
Fragments arranged by size:
2668 1881 1650 632 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1514 1546 2544 2576 6130 6162 6855
Size: 1514 32 998 32 3554 32 693
Fragments arranged by size:
3554 1514 998 693 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4387 6855
Size: 4387 2468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 514 2478 3240 3456 3762 4660 6855
Size: 514 1964 762 216 306 898 2195
Fragments arranged by size:
2195 1964 898 762 514 306 216
XmaI C'CCGG_G
Cuts at: 0 914 934 1090 6855
Size: 914 20 156 5765
Fragments arranged by size:
5765 914 156 20
XmnI GAAnn'nnTTC
Cuts at: 0 5300 6855
Size: 5300 1555
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApoI AseI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SalI SanDI SfcI SfoI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI ApaLI AscI AsiSI BmtI
BsaAI BsiWI BsmBI ClaI FseI FspI FspAI MfeI
MluI NheI NruI PacI PmeI PmlI PsiI PspXI
PsrI PvuI RsrII SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SrfI StuI SwaI XbaI XhoI
ZraI