Restriction map for
pF1KE2400
(Linear) (Six-base) MAPSORT of: KIEE2400.seq Check: 4196 from: 1 to: 2097
KIEE2400.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AatII G_ACGT'C
Cuts at: 0 918 2097
Size: 918 1179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 999 1775 1855 2097
Size: 64 935 776 80 242
Fragments arranged by size:
935 776 242 80 64
AfeI AGC'GCT
Cuts at: 0 331 2097
Size: 331 1766
AflIII A'CryG_T
Cuts at: 0 1204 2097
Size: 1204 893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1898 2097
Size: 1898 199
AlwNI CAG_nnn'CTG
Cuts at: 0 87 1057 2019 2097
Size: 87 970 962 78
Fragments arranged by size:
970 962 87 78
ApoI r'AATT_y
Cuts at: 0 250 349 706 1107 1631 2097
Size: 250 99 357 401 524 466
Fragments arranged by size:
524 466 401 357 250 99
AseI AT'TA_AT
Cuts at: 0 1445 2097
Size: 1445 652
AvaI C'yCGr_G
Cuts at: 0 583 2097
Size: 583 1514
BanI G'GyrC_C
Cuts at: 0 1962 2097
Size: 1962 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 1342 2097
Size: 1342 755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1944 1978 2097
Size: 1944 34 119
Fragments arranged by size:
1944 119 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1512 1917 2097
Size: 1512 405 180
Fragments arranged by size:
1512 405 180
BclI T'GATC_A
Cuts at: 0 657 1820 1826 2097
Size: 657 1163 6 271
Fragments arranged by size:
1163 657 271 6
Bme1580I G_kGCm'C
Cuts at: 0 1722 1967 2097
Size: 1722 245 130
Fragments arranged by size:
1722 245 130
BmgBI CAC'GTC
Cuts at: 0 1207 2097
Size: 1207 890
BmrI ACTGGGnnnn_n'
Cuts at: 0 299 2097
Size: 299 1798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 109 964 976 2097
Size: 79 30 855 12 1121
Fragments arranged by size:
1121 855 79 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 2097
Size: 1237 860
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 1924 2097
Size: 55 1869 173
Fragments arranged by size:
1869 173 55
BsaBI GATnn'nnATC
Cuts at: 0 1825 2097
Size: 1825 272
BsaHI Gr'CG_yC
Cuts at: 0 915 2097
Size: 915 1182
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1234 1385 2097
Size: 1234 151 712
Fragments arranged by size:
1234 712 151
BseYI C'CCAG_C
Cuts at: 0 326 2097
Size: 326 1771
BsmI GAATG_Cn'
Cuts at: 0 1869 1991 2097
Size: 1869 122 106
Fragments arranged by size:
1869 122 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 39 2097
Size: 39 2058
Bsp1286I G_dGCh'C
Cuts at: 0 1722 1967 2097
Size: 1722 245 130
Fragments arranged by size:
1722 245 130
BspHI T'CATG_A
Cuts at: 0 1001 1823 1832 2097
Size: 1001 822 9 265
Fragments arranged by size:
1001 822 265 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 796 2097
Size: 796 1301
BsrDI GCAATG_nn'
Cuts at: 0 141 2097
Size: 141 1956
BsrFI r'CCGG_y
Cuts at: 0 1151 2097
Size: 1151 946
BsrGI T'GTAC_A
Cuts at: 0 258 2097
Size: 258 1839
BssSI C'ACGA_G
Cuts at: 0 770 2097
Size: 770 1327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 788 2097
Size: 788 1309
BstBI TT'CG_AA
Cuts at: 0 428 2097
Size: 428 1669
BstYI r'GATC_y
Cuts at: 0 1139 1541 2097
Size: 1139 402 556
Fragments arranged by size:
1139 556 402
BtgI C'CryG_G
Cuts at: 0 2065 2097
Size: 2065 32
BtsI GCAGTG_nn'
Cuts at: 0 821 2097
Size: 821 1276
DraI TTT'AAA
Cuts at: 0 567 2097
Size: 567 1530
DraIII CAC_nnn'GTG
Cuts at: 0 716 1974 2097
Size: 716 1258 123
Fragments arranged by size:
1258 716 123
EaeI y'GGCC_r
Cuts at: 0 1469 2097
Size: 1469 628
EarI CTCTTCn'nnn_
Cuts at: 0 1074 1874 2097
Size: 1074 800 223
Fragments arranged by size:
1074 800 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 79 109 964 976 999 1775 1855
Size: 64 15 30 855 12 23 776 80
Cuts at: 1855 2097
Size: 242
Fragments arranged by size:
855 776 242 80 64 30 23 15
12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1029 1780 2097
Size: 1029 751 317
Fragments arranged by size:
1029 751 317
HaeII r_GCGC'y
Cuts at: 0 333 2097
Size: 333 1764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 415 447 1067 1099 1528 1560
Size: 38 32 345 32 620 32 429 32
Cuts at: 1560 2097
Size: 537
Fragments arranged by size:
620 537 429 345 38 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1086 2097
Size: 1086 1011
MfeI C'AATT_G
Cuts at: 0 452 538 2097
Size: 452 86 1559
Fragments arranged by size:
1559 452 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 2097
Size: 558 1539
MscI TGG'CCA
Cuts at: 0 1471 2097
Size: 1471 626
MslI CAynn'nnrTG
Cuts at: 0 1193 1445 1847 2097
Size: 1193 252 402 250
Fragments arranged by size:
1193 402 252 250
NaeI GCC'GGC
Cuts at: 0 1153 2097
Size: 1153 944
NgoMIV G'CCGG_C
Cuts at: 0 1151 2097
Size: 1151 946
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 1974 2097
Size: 540 1434 123
Fragments arranged by size:
1434 540 123
PfoI T'CCnGG_A
Cuts at: 0 56 1265 2097
Size: 56 1209 832
Fragments arranged by size:
1209 832 56
PstI C_TGCA'G
Cuts at: 0 948 2097
Size: 948 1149
SapI GCTCTTCn'nnn_
Cuts at: 0 1874 2097
Size: 1874 223
ScaI AGT'ACT
Cuts at: 0 1613 2097
Size: 1613 484
SfcI C'TryA_G
Cuts at: 0 675 944 2090 2097
Size: 675 269 1146 7
Fragments arranged by size:
1146 675 269 7
SmaI CCC'GGG
Cuts at: 0 585 2097
Size: 585 1512
SmlI C'TyrA_G
Cuts at: 0 1216 2097
Size: 1216 881
SspI AAT'ATT
Cuts at: 0 1040 2097
Size: 1040 1057
StuI AGG'CCT
Cuts at: 0 77 2097
Size: 77 2020
StyI C'CwwG_G
Cuts at: 0 264 515 2097
Size: 264 251 1582
Fragments arranged by size:
1582 264 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1102 2097
Size: 1102 995
TatI w'GTAC_w
Cuts at: 0 92 258 1474 1611 2097
Size: 92 166 1216 137 486
Fragments arranged by size:
1216 486 166 137 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 953 985 2097
Size: 953 32 1112
Fragments arranged by size:
1112 953 32
XmaI C'CCGG_G
Cuts at: 0 583 2097
Size: 583 1514
XmnI GAAnn'nnTTC
Cuts at: 0 215 2097
Size: 215 1882
ZraI GAC'GTC
Cuts at: 0 916 2097
Size: 916 1181
Enzymes that do cut:
AatII AcuI AfeI AflIII AlfI AlwNI ApoI AseI
AvaI BanI BbsI BcgI BciVI BclI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaBI BsaHI BseRI BseYI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtsI DraI DraIII
EaeI EarI Eco57MI FalI HaeII Hin4I HincII MfeI
MmeI MscI MslI NaeI NgoMIV PflMI PfoI PstI
SapI ScaI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BfrBI BglI BglII BlpI BmtI
BplI Bpu10I BsaAI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BspEI BsrBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XcmI XhoI