Restriction map for
pF1KE2406
(Linear) (Six-base) MAPSORT of: KIEE2406.seq Check: 4608 from: 1 to: 4491
KIEE2406.orf
With 182 enzymes: SgfI *
April 20, 2012 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 264 1065 4491
Size: 264 801 3426
Fragments arranged by size:
3426 801 264
Acc65I G'GTAC_C
Cuts at: 0 2371 4491
Size: 2371 2120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1249 1504 2830 2963 3272 4491
Size: 56 1193 255 1326 133 309 1219
Fragments arranged by size:
1326 1219 1193 309 255 133 56
AflIII A'CryG_T
Cuts at: 0 2969 3410 4491
Size: 2969 441 1081
Fragments arranged by size:
2969 1081 441
AhdI GACnn_n'nnGTC
Cuts at: 0 3603 4491
Size: 3603 888
AleI CACnn'nnGTG
Cuts at: 0 991 4491
Size: 991 3500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 4491
Size: 1012 32 3447
Fragments arranged by size:
3447 1012 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1987 2041 2518 3136 4491
Size: 1987 54 477 618 1355
Fragments arranged by size:
1987 1355 618 477 54
ApaI G_GGCC'C
Cuts at: 0 1784 2032 2990 2999 3319 3458 3683 4198
Size: 1784 248 958 9 320 139 225 515
Cuts at: 4198 4369 4491
Size: 171 122
Fragments arranged by size:
1784 958 515 320 248 225 171 139
122 9
ApoI r'AATT_y
Cuts at: 0 373 422 531 3514 4491
Size: 373 49 109 2983 977
Fragments arranged by size:
2983 977 373 109 49
AvaI C'yCGr_G
Cuts at: 0 2150 2206 2633 3320 3450 3546 3674 3684
Size: 2150 56 427 687 130 96 128 10
Cuts at: 3684 4406 4491
Size: 722 85
Fragments arranged by size:
2150 722 687 427 130 128 96 85
56 10
BamHI G'GATC_C
Cuts at: 0 3560 4491
Size: 3560 931
BanI G'GyrC_C
Cuts at: 0 613 889 1364 1581 2371 2506 3973 4491
Size: 613 276 475 217 790 135 1467 518
Fragments arranged by size:
1467 790 613 518 475 276 217 135
BanII G_rGCy'C
Cuts at: 0 249 337 523 598 727 1643 1784 2032
Size: 249 88 186 75 129 916 141 248
Cuts at: 2032 2060 2207 2412 2919 2990 2999 3016 3319
Size: 28 147 205 507 71 9 17 303
Cuts at: 3319 3458 3491 3683 3702 4198 4369 4491
Size: 139 33 192 19 496 171 122
Fragments arranged by size:
916 507 496 303 249 248 205 192
186 171 147 141 139 129 122 88
75 71 33 28 19 17 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 759 1160 1237 3260 4236 4491
Size: 759 401 77 2023 976 255
Fragments arranged by size:
2023 976 759 401 255 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4002 4036 4491
Size: 4002 34 455
Fragments arranged by size:
4002 455 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1783 4491
Size: 1783 2708
BclI T'GATC_A
Cuts at: 0 3749 4491
Size: 3749 742
BglI GCCn_nnn'nGGC
Cuts at: 0 1373 1574 1859 2027 2162 2522 2585 2604
Size: 1373 201 285 168 135 360 63 19
Cuts at: 2604 2669 2723 2732 2867 2994 3003 3678 3800
Size: 65 54 9 135 127 9 675 122
Cuts at: 3800 4202 4221 4491
Size: 402 19 270
Fragments arranged by size:
1373 675 402 360 285 270 201 168
135 135 127 122 65 63 54 19
19 9 9
BglII A'GATC_T
Cuts at: 0 3082 4107 4491
Size: 3082 1025 384
Fragments arranged by size:
3082 1025 384
Bme1580I G_kGCm'C
Cuts at: 0 1369 1784 2032 2511 2990 2999 3319 3458
Size: 1369 415 248 479 479 9 320 139
Cuts at: 3458 3683 4198 4342 4369 4491
Size: 225 515 144 27 122
Fragments arranged by size:
1369 515 479 479 415 320 248 225
144 139 122 27 9
BmgBI CAC'GTC
Cuts at: 0 3285 4491
Size: 3285 1206
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 2288 4491
Size: 594 1694 2203
Fragments arranged by size:
2203 1694 594
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3510 3618 4491
Size: 3510 108 873
Fragments arranged by size:
3510 873 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 321 1525 1555 1751 1940 2018 2336
Size: 175 146 1204 30 196 189 78 318
Cuts at: 2336 2396 2486 2498 3632 4124 4328 4491
Size: 60 90 12 1134 492 204 163
Fragments arranged by size:
1204 1134 492 318 204 196 189 175
163 146 90 78 60 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 4491
Size: 478 4013
BsaI GGTCTCn'nnnn_
Cuts at: 0 568 1217 2053 2312 2341 2539 2747 4491
Size: 568 649 836 259 29 198 208 1744
Fragments arranged by size:
1744 836 649 568 259 208 198 29
BsaAI yAC'GTr
Cuts at: 0 2593 2970 4491
Size: 2593 377 1521
Fragments arranged by size:
2593 1521 377
BsaHI Gr'CG_yC
Cuts at: 0 790 4491
Size: 790 3701
BsaWI w'CCGG_w
Cuts at: 0 354 3094 3737 4491
Size: 354 2740 643 754
Fragments arranged by size:
2740 754 643 354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 324 354 634 664 4458 4488 4491
Size: 324 30 280 30 3794 30 3
Fragments arranged by size:
3794 324 280 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 724 822 1322 1483 1533 1651 3481 3499
Size: 724 98 500 161 50 118 1830 18
Cuts at: 3499 3870 3873 3956 4491
Size: 371 3 83 535
Fragments arranged by size:
1830 724 535 500 371 161 118 98
83 50 18 3
BseYI C'CCAG_C
Cuts at: 0 865 1404 2526 4372 4453 4491
Size: 865 539 1122 1846 81 38
Fragments arranged by size:
1846 1122 865 539 81 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1015 1603 3971 3986 4491
Size: 966 49 588 2368 15 505
Fragments arranged by size:
2368 966 588 505 49 15
BsiEI CG_ry'CG
Cuts at: 0 508 4491
Size: 508 3983
BsiHKAI G_wGCw'C
Cuts at: 0 337 598 727 1643 2412 3491 3937 4491
Size: 337 261 129 916 769 1079 446 554
Fragments arranged by size:
1079 916 769 554 446 337 261 129
BsmI GAATG_Cn'
Cuts at: 0 2924 3398 4491
Size: 2924 474 1093
Fragments arranged by size:
2924 1093 474
Bsp1286I G_dGCh'C
Cuts at: 0 249 337 523 598 727 1369 1643 1784
Size: 249 88 186 75 129 642 274 141
Cuts at: 1784 2032 2060 2207 2412 2511 2919 2990 2999
Size: 248 28 147 205 99 408 71 9
Cuts at: 2999 3016 3319 3458 3491 3683 3702 3937 4198
Size: 17 303 139 33 192 19 235 261
Cuts at: 4198 4342 4369 4491
Size: 144 27 122
Fragments arranged by size:
642 408 303 274 261 249 248 235
205 192 186 147 144 141 139 129
122 99 88 75 71 33 28 27
19 17 9
BspEI T'CCGG_A
Cuts at: 0 354 3094 3737 4491
Size: 354 2740 643 754
Fragments arranged by size:
2740 754 643 354
BspHI T'CATG_A
Cuts at: 0 1085 4491
Size: 1085 3406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 264 1065 4491
Size: 264 801 3426
Fragments arranged by size:
3426 801 264
BsrDI GCAATG_nn'
Cuts at: 0 117 632 1454 2228 3576 4080 4491
Size: 117 515 822 774 1348 504 411
Fragments arranged by size:
1348 822 774 515 504 411 117
BsrFI r'CCGG_y
Cuts at: 0 149 2521 3622 4491
Size: 149 2372 1101 869
Fragments arranged by size:
2372 1101 869 149
BssSI C'ACGA_G
Cuts at: 0 212 410 1892 4491
Size: 212 198 1482 2599
Fragments arranged by size:
2599 1482 212 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3712 3973 4491
Size: 3712 261 518
Fragments arranged by size:
3712 518 261
BstEII G'GTnAC_C
Cuts at: 0 2128 4438 4491
Size: 2128 2310 53
Fragments arranged by size:
2310 2128 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4297 4491
Size: 4297 194
BstYI r'GATC_y
Cuts at: 0 1836 1915 3082 3560 4107 4491
Size: 1836 79 1167 478 547 384
Fragments arranged by size:
1836 1167 547 478 384 79
Bsu36I CC'TnA_GG
Cuts at: 0 2357 2817 4491
Size: 2357 460 1674
Fragments arranged by size:
2357 1674 460
BtgI C'CryG_G
Cuts at: 0 677 689 885 1586 1856 1883 2295 2483
Size: 677 12 196 701 270 27 412 188
Cuts at: 2483 3848 4491
Size: 1365 643
Fragments arranged by size:
1365 701 677 643 412 270 196 188
27 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1016 1116 4491
Size: 1016 100 3375
Fragments arranged by size:
3375 1016 100
BtsI GCAGTG_nn'
Cuts at: 0 1259 1293 1308 4491
Size: 1259 34 15 3183
Fragments arranged by size:
3183 1259 34 15
ClaI AT'CG_AT
Cuts at: 0 2443 4491
Size: 2443 2048
DraIII CAC_nnn'GTG
Cuts at: 0 1975 3192 4491
Size: 1975 1217 1299
Fragments arranged by size:
1975 1299 1217
DrdI GACnn_nn'nnGTC
Cuts at: 0 879 1814 3539 3659 4491
Size: 879 935 1725 120 832
Fragments arranged by size:
1725 935 879 832 120
EaeI y'GGCC_r
Cuts at: 0 2379 3030 4491
Size: 2379 651 1461
Fragments arranged by size:
2379 1461 651
EarI CTCTTCn'nnn_
Cuts at: 0 354 1450 3252 3930 4491
Size: 354 1096 1802 678 561
Fragments arranged by size:
1802 1096 678 561 354
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 963 1480 3317 4046 4094 4473 4491
Size: 963 517 1837 729 48 379 18
Fragments arranged by size:
1837 963 729 517 379 48 18
EcoICRI GAG'CTC
Cuts at: 0 335 596 725 1641 2410 3489 4491
Size: 335 261 129 916 769 1079 1002
Fragments arranged by size:
1079 1002 916 769 335 261 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 175 321 1249 1504 1525 1555 1751
Size: 56 119 146 928 255 21 30 196
Cuts at: 1751 1940 2018 2336 2396 2486 2498 2830 2963
Size: 189 78 318 60 90 12 332 133
Cuts at: 2963 3272 3632 4124 4328 4491
Size: 309 360 492 204 163
Fragments arranged by size:
928 492 360 332 318 309 255 204
196 189 163 146 133 119 90 78
60 56 30 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 2942 4491
Size: 294 2648 1549
Fragments arranged by size:
2648 1549 294
EcoO109I rG'GnC_Cy
Cuts at: 0 683 695 1735 1781 1852 1879 1888 1906
Size: 683 12 1040 46 71 27 9 18
Cuts at: 1906 1957 1993 2028 2029 2038 2119 2146 2227
Size: 51 36 35 1 9 81 27 81
Cuts at: 2227 2308 2344 2353 2461 2470 2488 2497 2515
Size: 81 36 9 108 9 18 9 18
Cuts at: 2515 2569 2605 2629 2743 2761 2770 2986 2987
Size: 54 36 24 114 18 9 216 1
Cuts at: 2987 2996 3106 3133 3316 3359 3454 3455 3541
Size: 9 110 27 183 43 95 1 86
Cuts at: 3541 3597 3643 3652 3670 3679 3680 3802 3820
Size: 56 46 9 18 9 1 122 18
Cuts at: 3820 4003 4161 4194 4312 4348 4365 4366 4491
Size: 183 158 33 118 36 17 1 125
Fragments arranged by size:
1040 683 216 183 183 158 125 122
118 114 110 108 95 86 81 81
81 71 56 54 51 46 46 43
36 36 36 36 35 33 27 27
27 24 18 18 18 18 18 18
17 12 9 9 9 9 9 9
9 9 9 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 531 3514 4491
Size: 531 2983 977
Fragments arranged by size:
2983 977 531
EcoRV GAT'ATC
Cuts at: 0 1269 4491
Size: 1269 3222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2092 2572 4491
Size: 2092 480 1919
Fragments arranged by size:
2092 1919 480
FspI TGC'GCA
Cuts at: 0 3778 4491
Size: 3778 713
HaeII r_GCGC'y
Cuts at: 0 1331 1357 4491
Size: 1331 26 3134
Fragments arranged by size:
3134 1331 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2425 2457 3303 3335 3589 3621 4326 4358
Size: 2425 32 846 32 254 32 705 32
Cuts at: 4358 4491
Size: 133
Fragments arranged by size:
2425 846 705 254 133 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 2375 4491
Size: 2375 2116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 2717 3510 4491
Size: 563 2154 793 981
Fragments arranged by size:
2154 981 793 563
MscI TGG'CCA
Cuts at: 0 3032 4491
Size: 3032 1459
MslI CAynn'nnrTG
Cuts at: 0 323 991 2968 3589 4491
Size: 323 668 1977 621 902
Fragments arranged by size:
1977 902 668 621 323
MspA1I CmG'CkG
Cuts at: 0 185 679 1100 1388 1588 2009 2530 3276
Size: 185 494 421 288 200 421 521 746
Cuts at: 3276 3718 4491
Size: 442 773
Fragments arranged by size:
773 746 521 494 442 421 421 288
200 185
NaeI GCC'GGC
Cuts at: 0 151 2523 3624 4491
Size: 151 2372 1101 867
Fragments arranged by size:
2372 1101 867 151
NcoI C'CATG_G
Cuts at: 0 689 885 1856 1883 2483 4491
Size: 689 196 971 27 600 2008
Fragments arranged by size:
2008 971 689 600 196 27
NdeI CA'TA_TG
Cuts at: 0 912 4491
Size: 912 3579
NgoMIV G'CCGG_C
Cuts at: 0 149 2521 3622 4491
Size: 149 2372 1101 869
Fragments arranged by size:
2372 1101 869 149
NspI r_CATG'y
Cuts at: 0 849 933 3066 3414 4065 4152 4491
Size: 849 84 2133 348 651 87 339
Fragments arranged by size:
2133 849 651 348 339 87 84
PasI CC'CwG_GG
Cuts at: 0 1759 1777 2349 2874 2992 3001 3667 3826
Size: 1759 18 572 525 118 9 666 159
Cuts at: 3826 3960 4318 4491
Size: 134 358 173
Fragments arranged by size:
1759 666 572 525 358 173 159 134
118 18 9
PciI A'CATG_T
Cuts at: 0 3410 4491
Size: 3410 1081
PflMI CCAn_nnn'nTGG
Cuts at: 0 461 1578 4491
Size: 461 1117 2913
Fragments arranged by size:
2913 1117 461
PfoI T'CCnGG_A
Cuts at: 0 632 1911 2475 3648 4344 4491
Size: 632 1279 564 1173 696 147
Fragments arranged by size:
1279 1173 696 632 564 147
PmlI CAC'GTG
Cuts at: 0 2593 4491
Size: 2593 1898
PpuMI rG'GwC_Cy
Cuts at: 0 683 695 1888 1957 1993 2038 2119 2146
Size: 683 12 1193 69 36 45 81 27
Cuts at: 2146 2227 2308 2344 2353 2470 2488 2497 2569
Size: 81 81 36 9 117 18 9 72
Cuts at: 2569 2629 2743 2761 3106 3133 3359 3541 3597
Size: 60 114 18 345 27 226 182 56
Cuts at: 3597 3652 3820 4003 4312 4348 4491
Size: 55 168 183 309 36 143
Fragments arranged by size:
1193 683 345 309 226 183 182 168
143 117 114 81 81 81 72 69
60 56 55 45 36 36 36 27
27 18 18 12 9 9
PshAI GACnn'nnGTC
Cuts at: 0 2759 3287 4001 4491
Size: 2759 528 714 490
Fragments arranged by size:
2759 714 528 490
PspOMI G'GGCC_C
Cuts at: 0 1780 2028 2986 2995 3315 3454 3679 4194
Size: 1780 248 958 9 320 139 225 515
Cuts at: 4194 4365 4491
Size: 171 126
Fragments arranged by size:
1780 958 515 320 248 225 171 139
126 9
PspXI vC'TCGA_Gb
Cuts at: 0 2150 3674 4406 4491
Size: 2150 1524 732 85
Fragments arranged by size:
2150 1524 732 85
PstI C_TGCA'G
Cuts at: 0 440 1266 1387 3275 3993 4491
Size: 440 826 121 1888 718 498
Fragments arranged by size:
1888 826 718 498 440 121
PvuI CG_AT'CG
Cuts at: 0 508 4491
Size: 508 3983
PvuII CAG'CTG
Cuts at: 0 1388 2530 3718 4491
Size: 1388 1142 1188 773
Fragments arranged by size:
1388 1188 1142 773
SacI G_AGCT'C
Cuts at: 0 337 598 727 1643 2412 3491 4491
Size: 337 261 129 916 769 1079 1000
Fragments arranged by size:
1079 1000 916 769 337 261 129
SacII CC_GC'GG
Cuts at: 0 680 1589 4491
Size: 680 909 2902
Fragments arranged by size:
2902 909 680
SanDI GG'GwC_CC
Cuts at: 0 1993 2488 3106 3359 3820 4003 4491
Size: 1993 495 618 253 461 183 488
Fragments arranged by size:
1993 618 495 488 461 253 183
SapI GCTCTTCn'nnn_
Cuts at: 0 1450 3930 4491
Size: 1450 2480 561
Fragments arranged by size:
2480 1450 561
ScaI AGT'ACT
Cuts at: 0 3307 4491
Size: 3307 1184
SexAI A'CCwGG_T
Cuts at: 0 1953 1971 3120 4380 4491
Size: 1953 18 1149 1260 111
Fragments arranged by size:
1953 1260 1149 111 18
SfcI C'TryA_G
Cuts at: 0 436 1262 1278 1383 2042 3271 3918 3989
Size: 436 826 16 105 659 1229 647 71
Cuts at: 3989 4254 4491
Size: 265 237
Fragments arranged by size:
1229 826 659 647 436 265 237 105
71 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1859 2027 2522 2585 2604 2669 2723 2732
Size: 1859 168 495 63 19 65 54 9
Cuts at: 2732 2867 2994 3003 3678 3800 4202 4221 4491
Size: 135 127 9 675 122 402 19 270
Fragments arranged by size:
1859 675 495 402 270 168 135 127
122 65 63 54 19 19 9 9
SmaI CCC'GGG
Cuts at: 0 2208 3452 3548 3686 4491
Size: 2208 1244 96 138 805
Fragments arranged by size:
2208 1244 805 138 96
SmlI C'TyrA_G
Cuts at: 0 493 2150 3674 4406 4491
Size: 493 1657 1524 732 85
Fragments arranged by size:
1657 1524 732 493 85
SphI G_CATG'C
Cuts at: 0 849 4152 4491
Size: 849 3303 339
Fragments arranged by size:
3303 849 339
StuI AGG'CCT
Cuts at: 0 1202 2156 2798 4491
Size: 1202 954 642 1693
Fragments arranged by size:
1693 1202 954 642
StyI C'CwwG_G
Cuts at: 0 689 885 1091 1342 1784 1856 1883 2052
Size: 689 196 206 251 442 72 27 169
Cuts at: 2052 2250 2483 2747 2757 2784 4491
Size: 198 233 264 10 27 1707
Fragments arranged by size:
1707 689 442 264 251 233 206 198
196 169 72 27 27 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 816 1808 3952 4491
Size: 816 992 2144 539
Fragments arranged by size:
2144 992 816 539
TatI w'GTAC_w
Cuts at: 0 3047 3059 3305 4491
Size: 3047 12 246 1186
Fragments arranged by size:
3047 1186 246 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 324 356 536 568 1961 1993 3545 3577
Size: 324 32 180 32 1393 32 1552 32
Cuts at: 3577 4491
Size: 914
Fragments arranged by size:
1552 1393 914 324 180 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 401 1236 4444 4491
Size: 401 835 3208 47
Fragments arranged by size:
3208 835 401 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 458 1068 1358 3572 3588 4450 4491
Size: 458 610 290 2214 16 862 41
Fragments arranged by size:
2214 862 610 458 290 41 16
XhoI C'TCGA_G
Cuts at: 0 2150 3674 4406 4491
Size: 2150 1524 732 85
Fragments arranged by size:
2150 1524 732 85
XmaI C'CCGG_G
Cuts at: 0 2206 3450 3546 3684 4491
Size: 2206 1244 96 138 807
Fragments arranged by size:
2206 1244 807 138 96
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII SacI SacII SanDI SapI ScaI SexAI
SfcI SfiI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BfrBI
BlpI BmtI Bpu10I BsaBI BsiWI BsmBI BsrBI BsrGI
BssHII BstBI BstZ17I CspCI DraI EagI FseI FspAI
HincII HindIII HpaI KasI MfeI MluI NarI NheI
NotI NruI NsiI PacI PmeI PpiI PsiI PsrI
RsrII SalI SbfI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XmnI ZraI