Restriction map for
pF1KE2416
(Linear) (Six-base) MAPSORT of: KIEE2416.seq Check: 6906 from: 1 to: 1917
KIEE2416.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 190 1099 1712 1917
Size: 190 909 613 205
Fragments arranged by size:
909 613 205 190
Acc65I G'GTAC_C
Cuts at: 0 1463 1917
Size: 1463 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1777 1917
Size: 1777 140
AfeI AGC'GCT
Cuts at: 0 1407 1917
Size: 1407 510
AleI CACnn'nnGTG
Cuts at: 0 601 1917
Size: 601 1316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1501 1917
Size: 1501 416
ApaI G_GGCC'C
Cuts at: 0 160 1650 1917
Size: 160 1490 267
Fragments arranged by size:
1490 267 160
ApaLI G'TGCA_C
Cuts at: 0 1086 1917
Size: 1086 831
AvaI C'yCGr_G
Cuts at: 0 123 1446 1696 1917
Size: 123 1323 250 221
Fragments arranged by size:
1323 250 221 123
BamHI G'GATC_C
Cuts at: 0 119 458 1069 1917
Size: 119 339 611 848
Fragments arranged by size:
848 611 339 119
BanI G'GyrC_C
Cuts at: 0 292 309 580 914 1225 1348 1463 1470
Size: 292 17 271 334 311 123 115 7
Cuts at: 1470 1700 1917
Size: 230 217
Fragments arranged by size:
334 311 292 271 230 217 123 115
17 7
BanII G_rGCy'C
Cuts at: 0 160 487 547 571 818 1055 1650 1814
Size: 160 327 60 24 247 237 595 164
Cuts at: 1814 1917
Size: 103
Fragments arranged by size:
595 327 247 237 164 160 103 60
24
BbeI G_GCGC'C
Cuts at: 0 296 1229 1917
Size: 296 933 688
Fragments arranged by size:
933 688 296
BbsI GAAGACnn'nnnn_
Cuts at: 0 1833 1917
Size: 1833 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1221 1255 1623 1657 1917
Size: 1221 34 368 34 260
Fragments arranged by size:
1221 368 260 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1462 1917
Size: 1462 455
BclI T'GATC_A
Cuts at: 0 1301 1917
Size: 1301 616
BglI GCCn_nnn'nGGC
Cuts at: 0 232 290 1369 1654 1917
Size: 232 58 1079 285 263
Fragments arranged by size:
1079 285 263 232 58
BglII A'GATC_T
Cuts at: 0 908 1917
Size: 908 1009
BlpI GC'TnA_GC
Cuts at: 0 173 361 1917
Size: 173 188 1556
Fragments arranged by size:
1556 188 173
Bme1580I G_kGCm'C
Cuts at: 0 98 160 1090 1475 1636 1650 1703 1917
Size: 98 62 930 385 161 14 53 214
Fragments arranged by size:
930 385 214 161 98 62 53 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 1342 1917
Size: 43 1299 575
Fragments arranged by size:
1299 575 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 984 1833 1917
Size: 561 423 849 84
Fragments arranged by size:
849 561 423 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 259 404 471 556 730 954 1867
Size: 223 36 145 67 85 174 224 913
Cuts at: 1867 1917
Size: 50
Fragments arranged by size:
913 224 223 174 145 85 67 50
36
Bpu10I CC'TnA_GC
Cuts at: 0 1808 1917
Size: 1808 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 784 1114 1917
Size: 289 495 330 803
Fragments arranged by size:
803 495 330 289
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 1917
Size: 525 1392
BsaAI yAC'GTr
Cuts at: 0 93 1917
Size: 93 1824
BsaHI Gr'CG_yC
Cuts at: 0 293 1226 1917
Size: 293 933 691
Fragments arranged by size:
933 691 293
BsaWI w'CCGG_w
Cuts at: 0 1035 1917
Size: 1035 882
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 377 407 910 940 1384 1414 1917
Size: 377 30 503 30 444 30 503
Fragments arranged by size:
503 503 444 377 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 259 513 1864 1917
Size: 256 3 254 1351 53
Fragments arranged by size:
1351 256 254 53 3
BseYI C'CCAG_C
Cuts at: 0 21 97 148 1509 1917
Size: 21 76 51 1361 408
Fragments arranged by size:
1361 408 76 51 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 715 1461 1917
Size: 499 216 746 456
Fragments arranged by size:
746 499 456 216
BsiHKAI G_wGCw'C
Cuts at: 0 179 487 547 1090 1670 1917
Size: 179 308 60 543 580 247
Fragments arranged by size:
580 543 308 247 179 60
Bsp1286I G_dGCh'C
Cuts at: 0 98 160 179 487 547 571 818 1055
Size: 98 62 19 308 60 24 247 237
Cuts at: 1055 1090 1475 1636 1650 1670 1703 1814 1917
Size: 35 385 161 14 20 33 111 103
Fragments arranged by size:
385 308 247 237 161 111 103 98
62 60 35 33 24 20 19 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 737 803 1099 1605 1712 1917
Size: 190 547 66 296 506 107 205
Fragments arranged by size:
547 506 296 205 190 107 66
BsrBI CCG'CTC
Cuts at: 0 1326 1817 1917
Size: 1326 491 100
Fragments arranged by size:
1326 491 100
BsrDI GCAATG_nn'
Cuts at: 0 863 1917
Size: 863 1054
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 1891 1917
Size: 69 1822 26
Fragments arranged by size:
1822 69 26
BstEII G'GTnAC_C
Cuts at: 0 465 1917
Size: 465 1452
BstXI CCAn_nnnn'nTGG
Cuts at: 0 382 438 564 982 1221 1497 1509 1917
Size: 382 56 126 418 239 276 12 408
Fragments arranged by size:
418 408 382 276 239 126 56 12
BstYI r'GATC_y
Cuts at: 0 119 458 908 1069 1917
Size: 119 339 450 161 848
Fragments arranged by size:
848 450 339 161 119
Bsu36I CC'TnA_GG
Cuts at: 0 104 886 1917
Size: 104 782 1031
Fragments arranged by size:
1031 782 104
BtgI C'CryG_G
Cuts at: 0 54 659 771 995 1214 1358 1601 1917
Size: 54 605 112 224 219 144 243 316
Fragments arranged by size:
605 316 243 224 219 144 112 54
BtsI GCAGTG_nn'
Cuts at: 0 217 1885 1917
Size: 217 1668 32
Fragments arranged by size:
1668 217 32
EaeI y'GGCC_r
Cuts at: 0 440 777 1142 1271 1361 1379 1499 1523
Size: 440 337 365 129 90 18 120 24
Cuts at: 1523 1917
Size: 394
Fragments arranged by size:
440 394 365 337 129 120 90 24
18
EarI CTCTTCn'nnn_
Cuts at: 0 343 1917
Size: 343 1574
EcoICRI GAG'CTC
Cuts at: 0 485 545 1917
Size: 485 60 1372
Fragments arranged by size:
1372 485 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 259 404 471 556 730 954 1777
Size: 223 36 145 67 85 174 224 823
Cuts at: 1777 1867 1917
Size: 90 50
Fragments arranged by size:
823 224 223 174 145 90 85 67
50 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 613 1129 1917
Size: 241 372 516 788
Fragments arranged by size:
788 516 372 241
EcoO109I rG'GnC_Cy
Cuts at: 0 26 108 128 157 198 1316 1646 1647
Size: 26 82 20 29 41 1118 330 1
Cuts at: 1647 1917
Size: 270
Fragments arranged by size:
1118 330 270 82 41 29 26 20
1
HaeII r_GCGC'y
Cuts at: 0 210 296 1229 1409 1510 1917
Size: 210 86 933 180 101 407
Fragments arranged by size:
933 407 210 180 101 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 1677 1709 1917
Size: 1384 32 261 32 208
Fragments arranged by size:
1384 261 208 32 32
KasI G'GCGC_C
Cuts at: 0 292 1225 1917
Size: 292 933 692
Fragments arranged by size:
933 692 292
KpnI G_GTAC'C
Cuts at: 0 1467 1917
Size: 1467 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 596 1364 1669 1917
Size: 455 141 768 305 248
Fragments arranged by size:
768 455 305 248 141
MscI TGG'CCA
Cuts at: 0 1144 1381 1501 1917
Size: 1144 237 120 416
Fragments arranged by size:
1144 416 237 120
MslI CAynn'nnrTG
Cuts at: 0 562 601 946 1018 1219 1495 1600 1901
Size: 562 39 345 72 201 276 105 301
Cuts at: 1901 1917
Size: 16
Fragments arranged by size:
562 345 301 276 201 105 72 39
16
MspA1I CmG'CkG
Cuts at: 0 378 840 1535 1827 1917
Size: 378 462 695 292 90
Fragments arranged by size:
695 462 378 292 90
NarI GG'CG_CC
Cuts at: 0 293 1226 1917
Size: 293 933 691
Fragments arranged by size:
933 691 293
NcoI C'CATG_G
Cuts at: 0 771 995 1214 1601 1917
Size: 771 224 219 387 316
Fragments arranged by size:
771 387 316 224 219
NspI r_CATG'y
Cuts at: 0 67 360 1917
Size: 67 293 1557
Fragments arranged by size:
1557 293 67
PasI CC'CwG_GG
Cuts at: 0 1388 1835 1917
Size: 1388 447 82
Fragments arranged by size:
1388 447 82
PflMI CCAn_nnn'nTGG
Cuts at: 0 55 1917
Size: 55 1862
PfoI T'CCnGG_A
Cuts at: 0 200 1844 1917
Size: 200 1644 73
Fragments arranged by size:
1644 200 73
PpuMI rG'GwC_Cy
Cuts at: 0 26 108 128 198 1917
Size: 26 82 20 70 1719
Fragments arranged by size:
1719 82 70 26 20
PspOMI G'GGCC_C
Cuts at: 0 156 1646 1917
Size: 156 1490 271
Fragments arranged by size:
1490 271 156
PstI C_TGCA'G
Cuts at: 0 1685 1917
Size: 1685 232
PvuII CAG'CTG
Cuts at: 0 378 840 1917
Size: 378 462 1077
Fragments arranged by size:
1077 462 378
SacI G_AGCT'C
Cuts at: 0 487 547 1917
Size: 487 60 1370
Fragments arranged by size:
1370 487 60
SanDI GG'GwC_CC
Cuts at: 0 26 1917
Size: 26 1891
SfcI C'TryA_G
Cuts at: 0 1681 1917
Size: 1681 236
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1654 1917
Size: 1654 263
SfoI GGC'GCC
Cuts at: 0 294 1227 1917
Size: 294 933 690
Fragments arranged by size:
933 690 294
SmaI CCC'GGG
Cuts at: 0 125 1698 1917
Size: 125 1573 219
Fragments arranged by size:
1573 219 125
SmlI C'TyrA_G
Cuts at: 0 304 799 1129 1917
Size: 304 495 330 788
Fragments arranged by size:
788 495 330 304
StuI AGG'CCT
Cuts at: 0 1806 1917
Size: 1806 111
StyI C'CwwG_G
Cuts at: 0 771 981 995 1145 1214 1502 1601 1917
Size: 771 210 14 150 69 288 99 316
Fragments arranged by size:
771 316 288 210 150 99 69 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 935 1279 1917
Size: 935 344 638
Fragments arranged by size:
935 638 344
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 377 409 910 942 1061 1093
Size: 35 32 310 32 501 32 119 32
Cuts at: 1093 1917
Size: 824
Fragments arranged by size:
824 501 310 119 35 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 600 1365 1842 1917
Size: 234 366 765 477 75
Fragments arranged by size:
765 477 366 234 75
XmaI C'CCGG_G
Cuts at: 0 123 1696 1917
Size: 123 1573 221
Fragments arranged by size:
1573 221 123
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BfrBI BmgBI BmtI BsaBI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI