Restriction map for
pF1KE2421
(Linear) (Six-base) MAPSORT of: KIEE2421.seq Check: 2231 from: 1 to: 7053
KIEE2421.orf
With 182 enzymes: SgfI *
October 17, 2012 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 7053
Size: 28 7025
AccI GT'mk_AC
Cuts at: 0 1034 7053
Size: 1034 6019
Acc65I G'GTAC_C
Cuts at: 0 1811 7053
Size: 1811 5242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1241 1673 2194 2550 4618 5005 6158
Size: 869 372 432 521 356 2068 387 1153
Cuts at: 6158 7053
Size: 895
Fragments arranged by size:
2068 1153 895 869 521 432 387 372
356
AflII C'TTAA_G
Cuts at: 0 319 7053
Size: 319 6734
AflIII A'CryG_T
Cuts at: 0 5202 5937 7053
Size: 5202 735 1116
Fragments arranged by size:
5202 1116 735
AleI CACnn'nnGTG
Cuts at: 0 6931 7053
Size: 6931 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2342 6092 7022 7053
Size: 2342 3750 930 31
Fragments arranged by size:
3750 2342 930 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 1861 1893 7053
Size: 316 32 1513 32 5160
Fragments arranged by size:
5160 1513 316 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 477 1603 2749 3709 7053
Size: 477 1126 1146 960 3344
Fragments arranged by size:
3344 1146 1126 960 477
ApaI G_GGCC'C
Cuts at: 0 3307 6198 7053
Size: 3307 2891 855
Fragments arranged by size:
3307 2891 855
ApaLI G'TGCA_C
Cuts at: 0 853 5596 6997 7053
Size: 853 4743 1401 56
Fragments arranged by size:
4743 1401 853 56
ApoI r'AATT_y
Cuts at: 0 214 561 637 1166 2289 3480 3523 3635
Size: 214 347 76 529 1123 1191 43 112
Cuts at: 3635 3720 3969 4458 5266 5680 6038 6408 6977
Size: 85 249 489 808 414 358 370 569
Cuts at: 6977 7053
Size: 76
Fragments arranged by size:
1191 1123 808 569 529 489 414 370
358 347 249 214 112 85 76 76
43
AseI AT'TA_AT
Cuts at: 0 3118 3667 7053
Size: 3118 549 3386
Fragments arranged by size:
3386 3118 549
AvaI C'yCGr_G
Cuts at: 0 731 4101 7053
Size: 731 3370 2952
Fragments arranged by size:
3370 2952 731
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 293 326 6489 6522 7053
Size: 293 33 6163 33 531
Fragments arranged by size:
6163 531 293 33 33
BamHI G'GATC_C
Cuts at: 0 1869 4743 7053
Size: 1869 2874 2310
Fragments arranged by size:
2874 2310 1869
BanI G'GyrC_C
Cuts at: 0 838 1637 1811 2129 5526 6285 6319 7053
Size: 838 799 174 318 3397 759 34 734
Fragments arranged by size:
3397 838 799 759 734 318 174 34
BanII G_rGCy'C
Cuts at: 0 14 582 2991 3307 5119 5223 5760 6198
Size: 14 568 2409 316 1812 104 537 438
Cuts at: 6198 6257 7053
Size: 59 796
Fragments arranged by size:
2409 1812 796 568 537 438 316 104
59 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 432 1094 2001 2057 2082 2365 6423
Size: 340 92 662 907 56 25 283 4058
Cuts at: 6423 6769 7053
Size: 346 284
Fragments arranged by size:
4058 907 662 346 340 284 283 92
56 25
BbvCI CC'TCA_GC
Cuts at: 0 290 1304 7053
Size: 290 1014 5749
Fragments arranged by size:
5749 1014 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6382 6416 7053
Size: 6382 34 637
Fragments arranged by size:
6382 637 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 375 3374 3456 4836 5829 7053
Size: 375 2999 82 1380 993 1224
Fragments arranged by size:
2999 1380 1224 993 375 82
BclI T'GATC_A
Cuts at: 0 402 4419 7053
Size: 402 4017 2634
Fragments arranged by size:
4017 2634 402
BfrBI ATG'CAT
Cuts at: 0 3931 7053
Size: 3931 3122
BglI GCCn_nnn'nGGC
Cuts at: 0 6062 7053
Size: 6062 991
BglII A'GATC_T
Cuts at: 0 1680 4210 7053
Size: 1680 2530 2843
Fragments arranged by size:
2843 2530 1680
BlpI GC'TnA_GC
Cuts at: 0 242 5890 7053
Size: 242 5648 1163
Fragments arranged by size:
5648 1163 242
Bme1580I G_kGCm'C
Cuts at: 0 841 857 3307 5600 6198 6324 7001 7053
Size: 841 16 2450 2293 598 126 677 52
Fragments arranged by size:
2450 2293 841 677 598 126 52 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1242 2653 3422 4065 7053
Size: 1242 1411 769 643 2988
Fragments arranged by size:
2988 1411 1242 769 643
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2468 4452 7053
Size: 2468 1984 2601
Fragments arranged by size:
2601 2468 1984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1874 2543 3776 6261 6321 6732 6828 7053
Size: 1874 669 1233 2485 60 411 96 225
Fragments arranged by size:
2485 1874 1233 669 411 225 96 60
Bpu10I CC'TnA_GC
Cuts at: 0 290 1304 5861 7053
Size: 290 1014 4557 1192
Fragments arranged by size:
4557 1192 1014 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1885 2889 4184 7053
Size: 1885 1004 1295 2869
Fragments arranged by size:
2869 1885 1295 1004
BsaI GGTCTCn'nnnn_
Cuts at: 0 782 4601 5704 6671 6708 7053
Size: 782 3819 1103 967 37 345
Fragments arranged by size:
3819 1103 967 782 345 37
BsaBI GATnn'nnATC
Cuts at: 0 1100 5108 6860 7053
Size: 1100 4008 1752 193
Fragments arranged by size:
4008 1752 1100 193
BsaHI Gr'CG_yC
Cuts at: 0 907 7053
Size: 907 6146
BsaWI w'CCGG_w
Cuts at: 0 6223 7053
Size: 6223 830
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1823 1853 2072 2102 4143 4173 6651 6674
Size: 1823 30 219 30 2041 30 2478 23
Cuts at: 6674 6681 6704 7053
Size: 7 23 349
Fragments arranged by size:
2478 2041 1823 349 219 30 30 30
23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1240 1699 2403 4197 5977 6714 7053
Size: 1240 459 704 1794 1780 737 339
Fragments arranged by size:
1794 1780 1240 737 704 459 339
BseYI C'CCAG_C
Cuts at: 0 6079 7053
Size: 6079 974
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1879 7053
Size: 101 1778 5174
Fragments arranged by size:
5174 1778 101
BsiHKAI G_wGCw'C
Cuts at: 0 14 857 1989 2991 3751 4108 4966 5600
Size: 14 843 1132 1002 760 357 858 634
Cuts at: 5600 6091 6247 7001 7053
Size: 491 156 754 52
Fragments arranged by size:
1132 1002 858 843 760 754 634 491
357 156 52 14
BsmI GAATG_Cn'
Cuts at: 0 3209 6058 6527 7053
Size: 3209 2849 469 526
Fragments arranged by size:
3209 2849 526 469
Bsp1286I G_dGCh'C
Cuts at: 0 14 582 841 857 1989 2991 3307 3751
Size: 14 568 259 16 1132 1002 316 444
Cuts at: 3751 4108 4966 5119 5223 5600 5760 6091 6198
Size: 357 858 153 104 377 160 331 107
Cuts at: 6198 6247 6257 6324 7001 7053
Size: 49 10 67 677 52
Fragments arranged by size:
1132 1002 858 677 568 444 377 357
331 316 259 160 153 107 104 67
52 49 16 14 10
BspEI T'CCGG_A
Cuts at: 0 6223 7053
Size: 6223 830
BspHI T'CATG_A
Cuts at: 0 3195 3725 7053
Size: 3195 530 3328
Fragments arranged by size:
3328 3195 530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 462 2734 3006 7053
Size: 28 434 2272 272 4047
Fragments arranged by size:
4047 2272 434 272 28
BsrDI GCAATG_nn'
Cuts at: 0 1211 4223 4596 5910 6447 7053
Size: 1211 3012 373 1314 537 606
Fragments arranged by size:
3012 1314 1211 606 537 373
BsrGI T'GTAC_A
Cuts at: 0 191 5981 6104 7053
Size: 191 5790 123 949
Fragments arranged by size:
5790 949 191 123
BssSI C'ACGA_G
Cuts at: 0 1370 4615 7053
Size: 1370 3245 2438
Fragments arranged by size:
3245 2438 1370
BstBI TT'CG_AA
Cuts at: 0 6975 7053
Size: 6975 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1009 2117 2587 3140 4740 6740 7053
Size: 1009 1108 470 553 1600 2000 313
Fragments arranged by size:
2000 1600 1108 1009 553 470 313
BstYI r'GATC_y
Cuts at: 0 1629 1680 1869 4210 4611 4743 5781 6276
Size: 1629 51 189 2341 401 132 1038 495
Cuts at: 6276 7053
Size: 777
Fragments arranged by size:
2341 1629 1038 777 495 401 189 132
51
Bsu36I CC'TnA_GG
Cuts at: 0 2766 3450 6719 7012 7053
Size: 2766 684 3269 293 41
Fragments arranged by size:
3269 2766 684 293 41
BtgI C'CryG_G
Cuts at: 0 5301 7053
Size: 5301 1752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 222 883 7053
Size: 222 661 6170
Fragments arranged by size:
6170 661 222
BtsI GCAGTG_nn'
Cuts at: 0 89 5168 5238 7053
Size: 89 5079 70 1815
Fragments arranged by size:
5079 1815 89 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2802 2837 4964 4999 7053
Size: 2802 35 2127 35 2054
Fragments arranged by size:
2802 2127 2054 35 35
DraI TTT'AAA
Cuts at: 0 2976 3174 5793 6569 7053
Size: 2976 198 2619 776 484
Fragments arranged by size:
2976 2619 776 484 198
EaeI y'GGCC_r
Cuts at: 0 608 4602 6206 6858 7053
Size: 608 3994 1604 652 195
Fragments arranged by size:
3994 1604 652 608 195
EarI CTCTTCn'nnn_
Cuts at: 0 999 1217 4299 4784 5024 6545 7053
Size: 999 218 3082 485 240 1521 508
Fragments arranged by size:
3082 1521 999 508 485 240 218
EcoICRI GAG'CTC
Cuts at: 0 12 2989 7053
Size: 12 2977 4064
Fragments arranged by size:
4064 2977 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1241 1673 1874 2194 2543 2550 3776
Size: 869 372 432 201 320 349 7 1226
Cuts at: 3776 4618 5005 6158 6261 6321 6732 6828 7053
Size: 842 387 1153 103 60 411 96 225
Fragments arranged by size:
1226 1153 869 842 432 411 387 372
349 320 225 201 103 96 60 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2612 4633 7053
Size: 2612 2021 2420
Fragments arranged by size:
2612 2420 2021
EcoO109I rG'GnC_Cy
Cuts at: 0 274 474 1405 2770 3303 3304 3424 4275
Size: 274 200 931 1365 533 1 120 851
Cuts at: 4275 4566 6195 7044 7053
Size: 291 1629 849 9
Fragments arranged by size:
1629 1365 931 851 849 533 291 274
200 120 9 1
EcoRI G'AATT_C
Cuts at: 0 214 561 2289 6977 7053
Size: 214 347 1728 4688 76
Fragments arranged by size:
4688 1728 347 214 76
EcoRV GAT'ATC
Cuts at: 0 3947 7053
Size: 3947 3106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 717 4645 6273 6700 7053
Size: 717 3928 1628 427 353
Fragments arranged by size:
3928 1628 717 427 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 882 914 1132 1164 2072 2104
Size: 709 32 141 32 218 32 908 32
Cuts at: 2104 2500 2532 3094 3126 3225 3257 3796 3828
Size: 396 32 562 32 99 32 539 32
Cuts at: 3828 4048 4080 4404 4436 4783 4815 5044 5076
Size: 220 32 324 32 347 32 229 32
Cuts at: 5076 5584 5616 5766 5798 6674 6706 7053
Size: 508 32 150 32 876 32 347
Fragments arranged by size:
908 876 709 562 539 508 396 347
347 324 229 220 218 150 141 99
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2954 3008 5580 7053
Size: 2954 54 2572 1473
Fragments arranged by size:
2954 2572 1473 54
HindIII A'AGCT_T
Cuts at: 0 377 1019 2277 7053
Size: 377 642 1258 4776
Fragments arranged by size:
4776 1258 642 377
KpnI G_GTAC'C
Cuts at: 0 1815 7053
Size: 1815 5238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1407 1913 3312 7053
Size: 1407 506 1399 3741
Fragments arranged by size:
3741 1407 1399 506
MscI TGG'CCA
Cuts at: 0 610 4604 6208 6860 7053
Size: 610 3994 1604 652 193
Fragments arranged by size:
3994 1604 652 610 193
MslI CAynn'nnrTG
Cuts at: 0 461 1004 2098 2585 3101 3280 5930 6074
Size: 461 543 1094 487 516 179 2650 144
Cuts at: 6074 6823 6931 7053
Size: 749 108 122
Fragments arranged by size:
2650 1094 749 543 516 487 461 179
144 122 108
MspA1I CmG'CkG
Cuts at: 0 1040 1308 1852 7053
Size: 1040 268 544 5201
Fragments arranged by size:
5201 1040 544 268
NdeI CA'TA_TG
Cuts at: 0 462 3245 3774 3786 5522 7053
Size: 462 2783 529 12 1736 1531
Fragments arranged by size:
2783 1736 1531 529 462 12
NsiI A_TGCA'T
Cuts at: 0 3933 7053
Size: 3933 3120
NspI r_CATG'y
Cuts at: 0 826 3935 5206 5941 6079 6585 6822 7053
Size: 826 3109 1271 735 138 506 237 231
Fragments arranged by size:
3109 1271 826 735 506 237 231 138
PasI CC'CwG_GG
Cuts at: 0 4440 6129 6315 7053
Size: 4440 1689 186 738
Fragments arranged by size:
4440 1689 738 186
PciI A'CATG_T
Cuts at: 0 5202 5937 7053
Size: 5202 735 1116
Fragments arranged by size:
5202 1116 735
PflMI CCAn_nnn'nTGG
Cuts at: 0 2521 5720 7053
Size: 2521 3199 1333
Fragments arranged by size:
3199 2521 1333
PfoI T'CCnGG_A
Cuts at: 0 5261 7053
Size: 5261 1792
PpuMI rG'GwC_Cy
Cuts at: 0 274 474 1405 2770 4275 4566 7044 7053
Size: 274 200 931 1365 1505 291 2478 9
Fragments arranged by size:
2478 1505 1365 931 291 274 200 9
PshAI GACnn'nnGTC
Cuts at: 0 1040 4273 7053
Size: 1040 3233 2780
Fragments arranged by size:
3233 2780 1040
PsiI TTA'TAA
Cuts at: 0 1277 7053
Size: 1277 5776
PspOMI G'GGCC_C
Cuts at: 0 3303 6194 7053
Size: 3303 2891 859
Fragments arranged by size:
3303 2891 859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1043 1075 7053
Size: 1043 32 5978
Fragments arranged by size:
5978 1043 32
PstI C_TGCA'G
Cuts at: 0 2658 5163 5755 7053
Size: 2658 2505 592 1298
Fragments arranged by size:
2658 2505 1298 592
PvuII CAG'CTG
Cuts at: 0 1040 1852 7053
Size: 1040 812 5201
Fragments arranged by size:
5201 1040 812
SacI G_AGCT'C
Cuts at: 0 14 2991 7053
Size: 14 2977 4062
Fragments arranged by size:
4062 2977 14
ScaI AGT'ACT
Cuts at: 0 3797 7053
Size: 3797 3256
SexAI A'CCwGG_T
Cuts at: 0 545 6661 7053
Size: 545 6116 392
Fragments arranged by size:
6116 545 392
SfcI C'TryA_G
Cuts at: 0 1604 1730 2654 3224 5159 5751 7053
Size: 1604 126 924 570 1935 592 1302
Fragments arranged by size:
1935 1604 1302 924 592 570 126
SmlI C'TyrA_G
Cuts at: 0 319 1864 2868 4199 7053
Size: 319 1545 1004 1331 2854
Fragments arranged by size:
2854 1545 1331 1004 319
SpeI A'CTAG_T
Cuts at: 0 585 7053
Size: 585 6468
SphI G_CATG'C
Cuts at: 0 3935 6585 7053
Size: 3935 2650 468
Fragments arranged by size:
3935 2650 468
SspI AAT'ATT
Cuts at: 0 863 1288 1580 6471 7053
Size: 863 425 292 4891 582
Fragments arranged by size:
4891 863 582 425 292
StuI AGG'CCT
Cuts at: 0 569 2618 5392 5606 7053
Size: 569 2049 2774 214 1447
Fragments arranged by size:
2774 2049 1447 569 214
StyI C'CwwG_G
Cuts at: 0 247 611 911 963 2368 4403 6248 6693
Size: 247 364 300 52 1405 2035 1845 445
Cuts at: 6693 7053
Size: 360
Fragments arranged by size:
2035 1845 1405 445 364 360 300 247
52
SwaI ATTT'AAAT
Cuts at: 0 6569 7053
Size: 6569 484
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 5544 6527 6984 7053
Size: 67 5477 983 457 69
Fragments arranged by size:
5477 983 457 69 67
TatI w'GTAC_w
Cuts at: 0 191 1322 2695 3795 5673 5981 6104 7053
Size: 191 1131 1373 1100 1878 308 123 949
Fragments arranged by size:
1878 1373 1131 1100 949 308 191 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2322 2354 6651 6683 7053
Size: 2322 32 4297 32 370
Fragments arranged by size:
4297 2322 370 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2444 7053
Size: 2444 4609
XbaI T'CTAG_A
Cuts at: 0 6945 7053
Size: 6945 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 254 1006 4401 4790 5818 7053
Size: 254 752 3395 389 1028 1235
Fragments arranged by size:
3395 1235 1028 752 389 254
XmnI GAAnn'nnTTC
Cuts at: 0 1379 3736 4006 4538 6138 6832 7053
Size: 1379 2357 270 532 1600 694 221
Fragments arranged by size:
2357 1600 1379 694 532 270 221
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BssSI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NdeI NsiI NspI
PasI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuII SacI ScaI SexAI SfcI SmlI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AscI AsiSI AvrII
BbeI BmgBI BmtI BsaAI BsiEI BsiWI BsmBI BsrBI
BsrFI BssHII BstAPI BstEII BstZ17I ClaI DraIII DrdI
EagI EciI FseI FspI FspAI HaeII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpiI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI XhoI XmaI ZraI