(Linear) (Six-base) MAPSORT of: KIEE2422.seq  Check: 8692  from: 1  to: 648



KIEE2422.orf

 With 182 enzymes: SgfI * 

                        October 5, 2012 11:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     502     648 
   Size:        164     338     146

  Fragments arranged by size: 

                 338     164     146
 
ApoI r'AATT_y

Cuts at:      0     426     574     648 
   Size:        426     148      74

  Fragments arranged by size: 

                 426     148      74
 
BanII G_rGCy'C

Cuts at:      0      83     648 
   Size:         83     565
 
BglII A'GATC_T

Cuts at:      0     153     648 
   Size:        153     495
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     648 
   Size:        314     334
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     328     648 
   Size:         83     245     320

  Fragments arranged by size: 

                 320     245      83
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     379     648 
   Size:        379     269
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     328     648 
   Size:         83     245     320

  Fragments arranged by size: 

                 320     245      83
 
BsrGI T'GTAC_A

Cuts at:      0     235     648 
   Size:        235     413
 
BstYI r'GATC_y

Cuts at:      0     153     648 
   Size:        153     495
 
BtsI GCAGTG_nn'

Cuts at:      0     117     648 
   Size:        117     531
 
DraI TTT'AAA

Cuts at:      0      39     648 
   Size:         39     609
 
EarI CTCTTCn'nnn_

Cuts at:      0      30     236     588     648 
   Size:         30     206     352      60

  Fragments arranged by size: 

                 352     206      60      30
 
EcoICRI GAG'CTC

Cuts at:      0      81     648 
   Size:         81     567
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     502     648 
   Size:        164     338     146

  Fragments arranged by size: 

                 338     164     146
 
EcoRI G'AATT_C

Cuts at:      0     574     648 
   Size:        574      74
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     121     648 
   Size:        121     527
 
HincII GTy'rAC

Cuts at:      0     177     648 
   Size:        177     471
 
HpaI GTT'AAC

Cuts at:      0     177     648 
   Size:        177     471
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     648 
   Size:        356     292
 
MslI CAynn'nnrTG

Cuts at:      0      74     436     648 
   Size:         74     362     212

  Fragments arranged by size: 

                 362     212      74
 
MspA1I CmG'CkG

Cuts at:      0     265     648 
   Size:        265     383
 
NspI r_CATG'y

Cuts at:      0     210     648 
   Size:        210     438
 
PfoI T'CCnGG_A

Cuts at:      0     569     648 
   Size:        569      79
 
PvuII CAG'CTG

Cuts at:      0     265     648 
   Size:        265     383
 
SacI G_AGCT'C

Cuts at:      0      83     648 
   Size:         83     565
 
SapI GCTCTTCn'nnn_

Cuts at:      0      30     648 
   Size:         30     618
 
TatI w'GTAC_w

Cuts at:      0     235     648 
   Size:        235     413
 

 Enzymes that do cut:

     AcuI     ApoI    BanII    BglII     BsgI  BsiHKAI    BsmBI Bsp1286I
    BsrGI    BstYI     BtsI     DraI     EarI  EcoICRI  Eco57MI    EcoRI
     FalI   HincII     HpaI     MmeI     MslI   MspA1I     NspI     PfoI
    PvuII     SacI     SapI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI