Restriction map for
pF1KE2427
(Linear) (Six-base) MAPSORT of: KIEE2427.seq Check: 7644 from: 1 to: 1893
KIEE2427.orf
With 182 enzymes: SgfI *
November 16, 2012 10:46 ..
AccI GT'mk_AC
Cuts at: 0 1775 1893
Size: 1775 118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 741 1133 1893
Size: 705 36 392 760
Fragments arranged by size:
760 705 392 36
AflIII A'CryG_T
Cuts at: 0 1370 1793 1893
Size: 1370 423 100
Fragments arranged by size:
1370 423 100
AgeI A'CCGG_T
Cuts at: 0 1067 1893
Size: 1067 826
AleI CACnn'nnGTG
Cuts at: 0 1618 1893
Size: 1618 275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1873 1893
Size: 1873 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1893
Size: 724 32 1137
Fragments arranged by size:
1137 724 32
AlwNI CAG_nnn'CTG
Cuts at: 0 59 492 778 897 1584 1893
Size: 59 433 286 119 687 309
Fragments arranged by size:
687 433 309 286 119 59
ApoI r'AATT_y
Cuts at: 0 138 1002 1018 1411 1574 1893
Size: 138 864 16 393 163 319
Fragments arranged by size:
864 393 319 163 138 16
AvaI C'yCGr_G
Cuts at: 0 580 1181 1893
Size: 580 601 712
Fragments arranged by size:
712 601 580
BamHI G'GATC_C
Cuts at: 0 1679 1893
Size: 1679 214
BanI G'GyrC_C
Cuts at: 0 3 192 1429 1690 1893
Size: 3 189 1237 261 203
Fragments arranged by size:
1237 261 203 189 3
BanII G_rGCy'C
Cuts at: 0 66 832 1094 1893
Size: 66 766 262 799
Fragments arranged by size:
799 766 262 66
BbeI G_GCGC'C
Cuts at: 0 7 1893
Size: 7 1886
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 970 1330 1893
Size: 18 952 360 563
Fragments arranged by size:
952 563 360 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1200 1234 1893
Size: 1200 34 659
Fragments arranged by size:
1200 659 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1383 1893
Size: 1383 510
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1870 1893
Size: 283 1587 23
Fragments arranged by size:
1587 283 23
BglII A'GATC_T
Cuts at: 0 226 302 327 371 1103 1893
Size: 226 76 25 44 732 790
Fragments arranged by size:
790 732 226 76 44 25
Bme1580I G_kGCm'C
Cuts at: 0 441 1213 1893
Size: 441 772 680
Fragments arranged by size:
772 680 441
BmgBI CAC'GTC
Cuts at: 0 99 1893
Size: 99 1794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 1893
Size: 762 1131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 943 1893
Size: 580 363 950
Fragments arranged by size:
950 580 363
Bpu10I CC'TnA_GC
Cuts at: 0 188 1893
Size: 188 1705
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 1893
Size: 313 1580
BsaAI yAC'GTr
Cuts at: 0 804 1893
Size: 804 1089
BsaBI GATnn'nnATC
Cuts at: 0 1154 1893
Size: 1154 739
BsaHI Gr'CG_yC
Cuts at: 0 4 70 1893
Size: 4 66 1823
Fragments arranged by size:
1823 66 4
BsaWI w'CCGG_w
Cuts at: 0 1067 1682 1893
Size: 1067 615 211
Fragments arranged by size:
1067 615 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1701 1731 1893
Size: 1701 30 162
Fragments arranged by size:
1701 162 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 546 559 1280 1893
Size: 478 68 13 721 613
Fragments arranged by size:
721 613 478 68 13
BseYI C'CCAG_C
Cuts at: 0 241 515 1194 1758 1865 1893
Size: 241 274 679 564 107 28
Fragments arranged by size:
679 564 274 241 107 28
BsiHKAI G_wGCw'C
Cuts at: 0 189 785 1094 1893
Size: 189 596 309 799
Fragments arranged by size:
799 596 309 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 1893
Size: 457 1436
Bsp1286I G_dGCh'C
Cuts at: 0 66 189 441 785 832 1094 1213 1893
Size: 66 123 252 344 47 262 119 680
Fragments arranged by size:
680 344 262 252 123 119 66 47
BspEI T'CCGG_A
Cuts at: 0 1682 1893
Size: 1682 211
BspHI T'CATG_A
Cuts at: 0 846 1007 1893
Size: 846 161 886
Fragments arranged by size:
886 846 161
BsrDI GCAATG_nn'
Cuts at: 0 252 1163 1893
Size: 252 911 730
Fragments arranged by size:
911 730 252
BsrFI r'CCGG_y
Cuts at: 0 1067 1893
Size: 1067 826
BsrGI T'GTAC_A
Cuts at: 0 1373 1893
Size: 1373 520
BstAPI GCAn_nnn'nTGC
Cuts at: 0 436 1893
Size: 436 1457
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 1327 1484 1893
Size: 249 1078 157 409
Fragments arranged by size:
1078 409 249 157
BstYI r'GATC_y
Cuts at: 0 226 302 327 371 1103 1679 1893
Size: 226 76 25 44 732 576 214
Fragments arranged by size:
732 576 226 214 76 44 25
Bsu36I CC'TnA_GG
Cuts at: 0 529 824 1517 1893
Size: 529 295 693 376
Fragments arranged by size:
693 529 376 295
BtgI C'CryG_G
Cuts at: 0 1145 1616 1893
Size: 1145 471 277
Fragments arranged by size:
1145 471 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1203 1893
Size: 1203 690
BtsI GCAGTG_nn'
Cuts at: 0 1634 1836 1893
Size: 1634 202 57
Fragments arranged by size:
1634 202 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1098 1133 1893
Size: 1098 35 760
Fragments arranged by size:
1098 760 35
DraIII CAC_nnn'GTG
Cuts at: 0 436 1893
Size: 436 1457
DrdI GACnn_nn'nnGTC
Cuts at: 0 1045 1542 1893
Size: 1045 497 351
Fragments arranged by size:
1045 497 351
EaeI y'GGCC_r
Cuts at: 0 1329 1817 1893
Size: 1329 488 76
Fragments arranged by size:
1329 488 76
EarI CTCTTCn'nnn_
Cuts at: 0 265 1597 1672 1893
Size: 265 1332 75 221
Fragments arranged by size:
1332 265 221 75
EcoICRI GAG'CTC
Cuts at: 0 1092 1893
Size: 1092 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 705 741 943 1133 1893
Size: 580 125 36 202 190 760
Fragments arranged by size:
760 580 202 190 125 36
EcoO109I rG'GnC_Cy
Cuts at: 0 285 555 1607 1893
Size: 285 270 1052 286
Fragments arranged by size:
1052 286 285 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 349 750 987 1893
Size: 349 401 237 906
Fragments arranged by size:
906 401 349 237
HaeII r_GCGC'y
Cuts at: 0 7 1893
Size: 7 1886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 287 312 319 343 344 375
Size: 60 32 195 25 7 24 1 31
Cuts at: 375 938 970 1088 1120 1408 1440 1893
Size: 563 32 118 32 288 32 453
Fragments arranged by size:
563 453 288 195 118 60 32 32
32 32 31 25 24 7 1
KasI G'GCGC_C
Cuts at: 0 3 1893
Size: 3 1890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1332 1727 1893
Size: 863 469 395 166
Fragments arranged by size:
863 469 395 166
MscI TGG'CCA
Cuts at: 0 1331 1819 1893
Size: 1331 488 74
Fragments arranged by size:
1331 488 74
MslI CAynn'nnrTG
Cuts at: 0 1618 1893
Size: 1618 275
MspA1I CmG'CkG
Cuts at: 0 343 705 775 1257 1893
Size: 343 362 70 482 636
Fragments arranged by size:
636 482 362 343 70
NarI GG'CG_CC
Cuts at: 0 4 1893
Size: 4 1889
NspI r_CATG'y
Cuts at: 0 215 928 1750 1797 1893
Size: 215 713 822 47 96
Fragments arranged by size:
822 713 215 96 47
PciI A'CATG_T
Cuts at: 0 1793 1893
Size: 1793 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 1338 1893
Size: 59 1279 555
Fragments arranged by size:
1279 555 59
PmlI CAC'GTG
Cuts at: 0 804 1893
Size: 804 1089
PpuMI rG'GwC_Cy
Cuts at: 0 555 1893
Size: 555 1338
PshAI GACnn'nnGTC
Cuts at: 0 1303 1893
Size: 1303 590
PstI C_TGCA'G
Cuts at: 0 497 774 1893
Size: 497 277 1119
Fragments arranged by size:
1119 497 277
PvuII CAG'CTG
Cuts at: 0 343 705 775 1257 1893
Size: 343 362 70 482 636
Fragments arranged by size:
636 482 362 343 70
SacI G_AGCT'C
Cuts at: 0 1094 1893
Size: 1094 799
SapI GCTCTTCn'nnn_
Cuts at: 0 1672 1893
Size: 1672 221
SfcI C'TryA_G
Cuts at: 0 493 770 980 1893
Size: 493 277 210 913
Fragments arranged by size:
913 493 277 210
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 283 1893
Size: 283 1610
SfoI GGC'GCC
Cuts at: 0 5 1893
Size: 5 1888
SmaI CCC'GGG
Cuts at: 0 1183 1893
Size: 1183 710
StyI C'CwwG_G
Cuts at: 0 180 413 1205 1893
Size: 180 233 792 688
Fragments arranged by size:
792 688 233 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1185 1893
Size: 1185 708
TatI w'GTAC_w
Cuts at: 0 1373 1893
Size: 1373 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1701 1733 1893
Size: 1701 32 160
Fragments arranged by size:
1701 160 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 96 1893
Size: 96 1797
XmaI C'CCGG_G
Cuts at: 0 1181 1893
Size: 1181 712
XmnI GAAnn'nnTTC
Cuts at: 0 1555 1893
Size: 1555 338
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlfI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NspI PciI PflMI PmlI PpuMI PshAI PstI PvuII
SacI SapI SfcI SfiI SfoI SmaI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BclI
BfrBI BlpI BmrI BmtI BpuEI BsgI BsiEI BsiWI
BsmI BspMI BsrBI BssHII BssSI BstBI BstEII BstZ17I
ClaI DraI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI ZraI