(Linear) (Six-base) MAPSORT of: KIEE2431.seq  Check: 8099  from: 1  to: 927



KIEE2431.orf

 With 182 enzymes: SgfI * 

                        September 11, 2018 18:37  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     287     927 
   Size:        287     640
 
ApoI r'AATT_y

Cuts at:      0      60      90     137     469     927 
   Size:         60      30      47     332     458

  Fragments arranged by size: 

                 458     332      60      47      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     919     927 
   Size:        771     148       8

  Fragments arranged by size: 

                 771     148       8
 
BbvCI CC'TCA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BclI T'GATC_A

Cuts at:      0     269     927 
   Size:        269     658
 
BglII A'GATC_T

Cuts at:      0     119     927 
   Size:        119     808
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     906     927 
   Size:        906      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     601     632     927 
   Size:        307     294      31     295

  Fragments arranged by size: 

                 307     295     294      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     927 
   Size:        162     765
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     906     927 
   Size:        906      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     927 
   Size:        381     546
 
BseYI C'CCAG_C

Cuts at:      0     655     927 
   Size:        655     272
 
BsrDI GCAATG_nn'

Cuts at:      0     518     927 
   Size:        518     409
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     927 
   Size:        119     524       8     276

  Fragments arranged by size: 

                 524     276     119       8
 
BtgI C'CryG_G

Cuts at:      0     539     633     927 
   Size:        539      94     294

  Fragments arranged by size: 

                 539     294      94
 
BtsI GCAGTG_nn'

Cuts at:      0     143     272     927 
   Size:        143     129     655

  Fragments arranged by size: 

                 655     143     129
 
EarI CTCTTCn'nnn_

Cuts at:      0     499     927 
   Size:        499     428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     927 
   Size:        403     524
 
EcoRV GAT'ATC

Cuts at:      0     740     927 
   Size:        740     187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141     927 
   Size:        141     786
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     906     927 
   Size:        104      32     770      21

  Fragments arranged by size: 

                 770     104      32      21
 
HindIII A'AGCT_T

Cuts at:      0     677     927 
   Size:        677     250
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     927 
   Size:        365     562
 
MslI CAynn'nnrTG

Cuts at:      0     388     927 
   Size:        388     539
 
NcoI C'CATG_G

Cuts at:      0     539     927 
   Size:        539     388
 
PsiI TTA'TAA

Cuts at:      0     254     927 
   Size:        254     673
 
SmlI C'TyrA_G

Cuts at:      0     322     616     647     927 
   Size:        322     294      31     280

  Fragments arranged by size: 

                 322     294     280      31
 
StyI C'CwwG_G

Cuts at:      0     539     927 
   Size:        539     388
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     839     888     927 
   Size:        839      49      39

  Fragments arranged by size: 

                 839      49      39
 
TatI w'GTAC_w

Cuts at:      0     515     927 
   Size:        515     412
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     927 
   Size:         36     891
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI     BclI    BglII     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI    BsrDI    BstYI
     BtgI     BtsI     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
  HindIII     MmeI     MslI     NcoI     PsiI     SmlI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI