Restriction map for
pF1KE2461
(Linear) (Six-base) MAPSORT of: KIEE2461.seq Check: 8499 from: 1 to: 1761
KIEE2461.orf
With 182 enzymes: SgfI *
March 15, 2013 17:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 1761
Size: 144 1617
AccI GT'mk_AC
Cuts at: 0 1583 1761
Size: 1583 178
Acc65I G'GTAC_C
Cuts at: 0 296 1233 1761
Size: 296 937 528
Fragments arranged by size:
937 528 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 1761
Size: 1512 249
AfeI AGC'GCT
Cuts at: 0 1369 1761
Size: 1369 392
AleI CACnn'nnGTG
Cuts at: 0 907 1021 1761
Size: 907 114 740
Fragments arranged by size:
907 740 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1615 1761
Size: 1615 146
AlwNI CAG_nnn'CTG
Cuts at: 0 737 1761
Size: 737 1024
ApaLI G'TGCA_C
Cuts at: 0 1395 1761
Size: 1395 366
AvaI C'yCGr_G
Cuts at: 0 169 1761
Size: 169 1592
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1217 1250 1761
Size: 1217 33 511
Fragments arranged by size:
1217 511 33
BanI G'GyrC_C
Cuts at: 0 296 489 711 749 870 1007 1233 1761
Size: 296 193 222 38 121 137 226 528
Fragments arranged by size:
528 296 226 222 193 137 121 38
BanII G_rGCy'C
Cuts at: 0 386 481 1069 1761
Size: 386 95 588 692
Fragments arranged by size:
692 588 386 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 1761
Size: 74 1687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1629 1663 1761
Size: 1629 34 98
Fragments arranged by size:
1629 98 34
BclI T'GATC_A
Cuts at: 0 1289 1761
Size: 1289 472
BglI GCCn_nnn'nGGC
Cuts at: 0 26 1097 1761
Size: 26 1071 664
Fragments arranged by size:
1071 664 26
BlpI GC'TnA_GC
Cuts at: 0 475 1296 1627 1761
Size: 475 821 331 134
Fragments arranged by size:
821 475 331 134
Bme1580I G_kGCm'C
Cuts at: 0 494 752 800 873 1399 1761
Size: 494 258 48 73 526 362
Fragments arranged by size:
526 494 362 258 73 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 415 589 951 1425 1761
Size: 130 285 174 362 474 336
Fragments arranged by size:
474 362 336 285 174 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1761
Size: 638 1123
Bpu10I CC'TnA_GC
Cuts at: 0 1636 1761
Size: 1636 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1411 1761
Size: 1411 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 386 656 670 1396 1761
Size: 386 270 14 726 365
Fragments arranged by size:
726 386 365 270 14
BsaAI yAC'GTr
Cuts at: 0 1677 1761
Size: 1677 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 636 666 1761
Size: 636 30 1095
Fragments arranged by size:
1095 636 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 1438 1761
Size: 265 1173 323
Fragments arranged by size:
1173 323 265
BseYI C'CCAG_C
Cuts at: 0 53 808 1062 1304 1621 1761
Size: 53 755 254 242 317 140
Fragments arranged by size:
755 317 254 242 140 53
BsiHKAI G_wGCw'C
Cuts at: 0 356 386 932 1399 1715 1761
Size: 356 30 546 467 316 46
Fragments arranged by size:
546 467 356 316 46 30
Bsp1286I G_dGCh'C
Cuts at: 0 356 386 481 494 752 800 873 932
Size: 356 30 95 13 258 48 73 59
Cuts at: 932 1069 1399 1715 1761
Size: 137 330 316 46
Fragments arranged by size:
356 330 316 258 137 95 73 59
48 46 30 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 898 1069 1607 1761
Size: 144 754 171 538 154
Fragments arranged by size:
754 538 171 154 144
BssSI C'ACGA_G
Cuts at: 0 1740 1761
Size: 1740 21
BstEII G'GTnAC_C
Cuts at: 0 174 1761
Size: 174 1587
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 853 1273 1413 1617 1761
Size: 501 352 420 140 204 144
Fragments arranged by size:
501 420 352 204 144 140
BstYI r'GATC_y
Cuts at: 0 509 567 1761
Size: 509 58 1194
Fragments arranged by size:
1194 509 58
Bsu36I CC'TnA_GG
Cuts at: 0 875 1761
Size: 875 886
BtgI C'CryG_G
Cuts at: 0 266 1266 1571 1761
Size: 266 1000 305 190
Fragments arranged by size:
1000 305 266 190
BtsI GCAGTG_nn'
Cuts at: 0 431 777 1345 1761
Size: 431 346 568 416
Fragments arranged by size:
568 431 416 346
DraIII CAC_nnn'GTG
Cuts at: 0 519 1761
Size: 519 1242
EaeI y'GGCC_r
Cuts at: 0 1574 1607 1761
Size: 1574 33 154
Fragments arranged by size:
1574 154 33
EarI CTCTTCn'nnn_
Cuts at: 0 229 857 1543 1761
Size: 229 628 686 218
Fragments arranged by size:
686 628 229 218
EcoICRI GAG'CTC
Cuts at: 0 384 1761
Size: 384 1377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1512 1761
Size: 638 874 249
Fragments arranged by size:
874 638 249
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 1483 1761
Size: 226 1257 278
Fragments arranged by size:
1257 278 226
EcoO109I rG'GnC_Cy
Cuts at: 0 812 1143 1761
Size: 812 331 618
Fragments arranged by size:
812 618 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 412 639 1761
Size: 412 227 1122
Fragments arranged by size:
1122 412 227
HaeII r_GCGC'y
Cuts at: 0 1371 1749 1761
Size: 1371 378 12
Fragments arranged by size:
1371 378 12
KpnI G_GTAC'C
Cuts at: 0 300 1237 1761
Size: 300 937 524
Fragments arranged by size:
937 524 300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1761
Size: 1224 537
MslI CAynn'nnrTG
Cuts at: 0 907 1021 1761
Size: 907 114 740
Fragments arranged by size:
907 740 114
MspA1I CmG'CkG
Cuts at: 0 303 1300 1308 1643 1761
Size: 303 997 8 335 118
Fragments arranged by size:
997 335 303 118 8
PasI CC'CwG_GG
Cuts at: 0 291 1413 1761
Size: 291 1122 348
Fragments arranged by size:
1122 348 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 759 1761
Size: 759 1002
PfoI T'CCnGG_A
Cuts at: 0 1552 1761
Size: 1552 209
PpuMI rG'GwC_Cy
Cuts at: 0 812 1761
Size: 812 949
PstI C_TGCA'G
Cuts at: 0 35 155 1474 1761
Size: 35 120 1319 287
Fragments arranged by size:
1319 287 120 35
PvuII CAG'CTG
Cuts at: 0 303 1308 1761
Size: 303 1005 453
Fragments arranged by size:
1005 453 303
SacI G_AGCT'C
Cuts at: 0 386 1761
Size: 386 1375
SapI GCTCTTCn'nnn_
Cuts at: 0 1543 1761
Size: 1543 218
SexAI A'CCwGG_T
Cuts at: 0 707 1761
Size: 707 1054
SfcI C'TryA_G
Cuts at: 0 31 151 837 1470 1761
Size: 31 120 686 633 291
Fragments arranged by size:
686 633 291 120 31
SmlI C'TyrA_G
Cuts at: 0 1390 1761
Size: 1390 371
StuI AGG'CCT
Cuts at: 0 125 1122 1723 1761
Size: 125 997 601 38
Fragments arranged by size:
997 601 125 38
StyI C'CwwG_G
Cuts at: 0 387 1155 1229 1761
Size: 387 768 74 532
Fragments arranged by size:
768 532 387 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 930 1761
Size: 930 831
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 636 668 1761
Size: 636 32 1093
Fragments arranged by size:
1093 636 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 1761
Size: 288 1473
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaLI AvaI BaeI BanI BanII BbsI BcgI BclI
BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KpnI
MmeI MslI MspA1I PasI PflMI PfoI PpuMI PstI
PvuII SacI SapI SexAI SfcI SmlI StuI StyI
TaqII TstI XcmI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApaI
ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI
BciVI BfrBI BglII BmgBI BmrI BmtI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI