(Linear) (Six-base) MAPSORT of: KIEE2463.seq  Check: 2301  from: 1  to: 639



KIEE2463.orf

 With 182 enzymes: SgfI * 

                        March 1, 2013 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      24     639 
   Size:         24     615
 
AccI GT'mk_AC

Cuts at:      0     101     639 
   Size:        101     538
 
Acc65I G'GTAC_C

Cuts at:      0     414     639 
   Size:        414     225
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     226     639 
   Size:         30     196     413

  Fragments arranged by size: 

                 413     196      30
 
AflIII A'CryG_T

Cuts at:      0     584     639 
   Size:        584      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     612     639 
   Size:        612      27
 
BanI G'GyrC_C

Cuts at:      0     414     639 
   Size:        414     225
 
BanII G_rGCy'C

Cuts at:      0      26     639 
   Size:         26     613
 
BclI T'GATC_A

Cuts at:      0     323     639 
   Size:        323     316
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     318     320     639 
   Size:        318       2     319

  Fragments arranged by size: 

                 319     318       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     639 
   Size:        184     455
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     639 
   Size:        466     173
 
BseYI C'CCAG_C

Cuts at:      0       9     612     639 
   Size:          9     603      27

  Fragments arranged by size: 

                 603      27       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     639 
   Size:         26     613
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24     639 
   Size:         24     615
 
BstZ17I GTA'TAC

Cuts at:      0     102     639 
   Size:        102     537
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      54     388     639 
   Size:         54     334     251

  Fragments arranged by size: 

                 334     251      54
 
BtsI GCAGTG_nn'

Cuts at:      0      20     639 
   Size:         20     619
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     534     589     639 
   Size:        534      55      50

  Fragments arranged by size: 

                 534      55      50
 
EaeI y'GGCC_r

Cuts at:      0       2     639 
   Size:          2     637
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     184     226     639 
   Size:         30     154      42     413

  Fragments arranged by size: 

                 413     154      42      30
 
FspI TGC'GCA

Cuts at:      0     562     639 
   Size:        562      77
 
FspAI rTGC'GCAy

Cuts at:      0     562     639 
   Size:        562      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419     639 
   Size:        387      32     220

  Fragments arranged by size: 

                 387     220      32
 
HincII GTy'rAC

Cuts at:      0     582     639 
   Size:        582      57
 
HindIII A'AGCT_T

Cuts at:      0      76     639 
   Size:         76     563
 
KpnI G_GTAC'C

Cuts at:      0     418     639 
   Size:        418     221
 
MslI CAynn'nnrTG

Cuts at:      0     262     639 
   Size:        262     377
 
NspI r_CATG'y

Cuts at:      0     588     639 
   Size:        588      51
 
PciI A'CATG_T

Cuts at:      0     584     639 
   Size:        584      55
 
SmlI C'TyrA_G

Cuts at:      0     481     639 
   Size:        481     158
 
StuI AGG'CCT

Cuts at:      0      87     639 
   Size:         87     552
 
StyI C'CwwG_G

Cuts at:      0     363     639 
   Size:        363     276
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     535     639 
   Size:        535     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     542     574     639 
   Size:        542      32      65

  Fragments arranged by size: 

                 542      65      32
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII    AlwNI     BanI    BanII
     BclI     BplI     BpmI    BpuEI    BseYI Bsp1286I    BspMI  BstZ17I
    BtgZI     BtsI     DrdI     EaeI  Eco57MI     FspI    FspAI    Hin4I
   HincII  HindIII     KpnI     MslI     NspI     PciI     SmlI     StuI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     ClaI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    HaeII     HpaI     KasI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI