(Linear) (Six-base) MAPSORT of: KIEE2464.seq Check: 3484 from: 1 to: 2289 KIEE2464.orf With 182 enzymes: SgfI * March 1, 2013 10:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1965 2289 Size: 1965 324 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 232 370 751 985 1127 1354 1417 Size: 71 161 138 381 234 142 227 63 Cuts at: 1417 1630 1813 2289 Size: 213 183 476 Fragments arranged by size: 476 381 234 227 213 183 161 142 138 71 63 AflIII A'CryG_T Cuts at: 0 1071 2219 2289 Size: 1071 1148 70 Fragments arranged by size: 1148 1071 70 AlwNI CAG_nnn'CTG Cuts at: 0 579 808 1395 2289 Size: 579 229 587 894 Fragments arranged by size: 894 587 579 229 AvaI C'yCGr_G Cuts at: 0 1381 2289 Size: 1381 908 BanII G_rGCy'C Cuts at: 0 764 1026 2289 Size: 764 262 1263 Fragments arranged by size: 1263 764 262 BbsI GAAGACnn'nnnn_ Cuts at: 0 1262 1462 2289 Size: 1262 200 827 Fragments arranged by size: 1262 827 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1039 1073 1695 1729 2289 Size: 1039 34 622 34 560 Fragments arranged by size: 1039 622 560 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 483 2289 Size: 483 1806 BglII A'GATC_T Cuts at: 0 1212 2289 Size: 1212 1077 BlpI GC'TnA_GC Cuts at: 0 580 1061 2053 2289 Size: 580 481 992 236 Fragments arranged by size: 992 580 481 236 Bme1580I G_kGCm'C Cuts at: 0 1836 2289 Size: 1836 453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 720 2289 Size: 720 1569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 286 307 931 1152 1258 1339 1756 Size: 88 198 21 624 221 106 81 417 Cuts at: 1756 1828 1840 1924 2170 2289 Size: 72 12 84 246 119 Fragments arranged by size: 624 417 246 221 198 119 106 88 84 81 72 21 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 776 1195 2062 2289 Size: 776 419 867 227 Fragments arranged by size: 867 776 419 227 BsaI GGTCTCn'nnnn_ Cuts at: 0 105 589 608 980 1163 2038 2289 Size: 105 484 19 372 183 875 251 Fragments arranged by size: 875 484 372 251 183 105 19 BsaAI yAC'GTr Cuts at: 0 1074 2289 Size: 1074 1215 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 1987 2017 2244 2274 2289 Size: 400 30 1557 30 227 30 15 Fragments arranged by size: 1557 400 227 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 304 379 607 1066 1149 1255 1888 1921 Size: 304 75 228 459 83 106 633 33 Cuts at: 1921 1983 2270 2289 Size: 62 287 19 Fragments arranged by size: 633 459 304 287 228 106 83 75 62 33 19 BseYI C'CCAG_C Cuts at: 0 1487 1892 2048 2289 Size: 1487 405 156 241 Fragments arranged by size: 1487 405 241 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 2289 Size: 513 1776 BsiHKAI G_wGCw'C Cuts at: 0 323 586 678 764 1997 2167 2289 Size: 323 263 92 86 1233 170 122 Fragments arranged by size: 1233 323 263 170 122 92 86 BsmI GAATG_Cn' Cuts at: 0 201 1309 2289 Size: 201 1108 980 Fragments arranged by size: 1108 980 201 Bsp1286I G_dGCh'C Cuts at: 0 323 586 678 764 1026 1836 1997 2167 Size: 323 263 92 86 262 810 161 170 Cuts at: 2167 2289 Size: 122 Fragments arranged by size: 810 323 263 262 170 161 122 92 86 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 462 535 1595 1709 1965 2289 Size: 462 73 1060 114 256 324 Fragments arranged by size: 1060 462 324 256 114 73 BsrBI CCG'CTC Cuts at: 0 1665 2270 2289 Size: 1665 605 19 Fragments arranged by size: 1665 605 19 BsrDI GCAATG_nn' Cuts at: 0 60 2289 Size: 60 2229 BsrFI r'CCGG_y Cuts at: 0 2123 2289 Size: 2123 166 BssHII G'CGCG_C Cuts at: 0 715 2289 Size: 715 1574 BssSI C'ACGA_G Cuts at: 0 322 2289 Size: 322 1967 BstXI CCAn_nnnn'nTGG Cuts at: 0 435 2289 Size: 435 1854 BstYI r'GATC_y Cuts at: 0 1092 1212 1517 2289 Size: 1092 120 305 772 Fragments arranged by size: 1092 772 305 120 Bsu36I CC'TnA_GG Cuts at: 0 622 2289 Size: 622 1667 BtgI C'CryG_G Cuts at: 0 824 2289 Size: 824 1465 BtsI GCAGTG_nn' Cuts at: 0 633 676 2289 Size: 633 43 1613 Fragments arranged by size: 1613 633 43 DrdI GACnn_nn'nnGTC Cuts at: 0 1286 2289 Size: 1286 1003 EaeI y'GGCC_r Cuts at: 0 251 2289 Size: 251 2038 EarI CTCTTCn'nnn_ Cuts at: 0 275 752 1039 2289 Size: 275 477 287 1250 Fragments arranged by size: 1250 477 287 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 42 457 709 2103 2289 Size: 42 415 252 1394 186 Fragments arranged by size: 1394 415 252 186 42 EcoICRI GAG'CTC Cuts at: 0 762 2289 Size: 762 1527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 88 232 286 307 370 751 931 Size: 71 17 144 54 21 63 381 180 Cuts at: 931 985 1127 1152 1258 1339 1354 1417 1630 Size: 54 142 25 106 81 15 63 213 Cuts at: 1630 1756 1813 1828 1840 1924 2170 2289 Size: 126 57 15 12 84 246 119 Fragments arranged by size: 381 246 213 180 144 142 126 119 106 84 81 71 63 63 57 54 54 25 21 17 15 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 403 2289 Size: 403 1886 EcoO109I rG'GnC_Cy Cuts at: 0 1928 2289 Size: 1928 361 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 781 1359 2289 Size: 781 578 930 Fragments arranged by size: 930 781 578 FspI TGC'GCA Cuts at: 0 1705 2289 Size: 1705 584 HaeII r_GCGC'y Cuts at: 0 246 2289 Size: 246 2043 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 127 159 171 203 891 923 2244 2276 Size: 127 32 12 32 688 32 1321 32 Cuts at: 2276 2289 Size: 13 Fragments arranged by size: 1321 688 127 32 32 32 32 13 12 HindIII A'AGCT_T Cuts at: 0 1109 2289 Size: 1109 1180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1925 2289 Size: 1925 364 MscI TGG'CCA Cuts at: 0 253 2289 Size: 253 2036 MslI CAynn'nnrTG Cuts at: 0 1920 2289 Size: 1920 369 MspA1I CmG'CkG Cuts at: 0 920 963 1395 1578 1785 1896 2052 2082 Size: 920 43 432 183 207 111 156 30 Cuts at: 2082 2289 Size: 207 Fragments arranged by size: 920 432 207 207 183 156 111 43 30 NaeI GCC'GGC Cuts at: 0 2125 2289 Size: 2125 164 NcoI C'CATG_G Cuts at: 0 824 2289 Size: 824 1465 NgoMIV G'CCGG_C Cuts at: 0 2123 2289 Size: 2123 166 NspI r_CATG'y Cuts at: 0 897 2181 2289 Size: 897 1284 108 Fragments arranged by size: 1284 897 108 PflMI CCAn_nnn'nTGG Cuts at: 0 306 2289 Size: 306 1983 PfoI T'CCnGG_A Cuts at: 0 263 1129 2033 2289 Size: 263 866 904 256 Fragments arranged by size: 904 866 263 256 PmlI CAC'GTG Cuts at: 0 1074 2289 Size: 1074 1215 PpuMI rG'GwC_Cy Cuts at: 0 1928 2289 Size: 1928 361 PshAI GACnn'nnGTC Cuts at: 0 146 2289 Size: 146 2143 PspXI vC'TCGA_Gb Cuts at: 0 1381 2289 Size: 1381 908 PstI C_TGCA'G Cuts at: 0 575 1592 1655 2289 Size: 575 1017 63 634 Fragments arranged by size: 1017 634 575 63 PvuII CAG'CTG Cuts at: 0 963 1395 1578 1785 1896 2052 2082 2289 Size: 963 432 183 207 111 156 30 207 Fragments arranged by size: 963 432 207 207 183 156 111 30 RsrII CG'GwC_CG Cuts at: 0 2106 2289 Size: 2106 183 SacI G_AGCT'C Cuts at: 0 764 2289 Size: 764 1525 SanDI GG'GwC_CC Cuts at: 0 1928 2289 Size: 1928 361 SapI GCTCTTCn'nnn_ Cuts at: 0 275 752 1039 2289 Size: 275 477 287 1250 Fragments arranged by size: 1250 477 287 275 SfcI C'TryA_G Cuts at: 0 128 571 1588 1651 1852 2289 Size: 128 443 1017 63 201 437 Fragments arranged by size: 1017 443 437 201 128 63 SmlI C'TyrA_G Cuts at: 0 791 1174 1381 2041 2289 Size: 791 383 207 660 248 Fragments arranged by size: 791 660 383 248 207 SphI G_CATG'C Cuts at: 0 897 2181 2289 Size: 897 1284 108 Fragments arranged by size: 1284 897 108 StuI AGG'CCT Cuts at: 0 1402 2289 Size: 1402 887 StyI C'CwwG_G Cuts at: 0 824 1988 2006 2289 Size: 824 1164 18 283 Fragments arranged by size: 1164 824 283 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 851 1004 2289 Size: 851 153 1285 Fragments arranged by size: 1285 851 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 400 432 1987 2019 2289 Size: 400 32 1555 32 270 Fragments arranged by size: 1555 400 270 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 261 474 2289 Size: 261 213 1815 Fragments arranged by size: 1815 261 213 XhoI C'TCGA_G Cuts at: 0 1381 2289 Size: 1381 908 XmnI GAAnn'nnTTC Cuts at: 0 373 2289 Size: 373 1916 Enzymes that do cut: AarI AcuI AflIII AlwNI AvaI BanII BbsI BcgI BciVI BglII BlpI Bme1580I BplI BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI PshAI PspXI PstI PvuII RsrII SacI SanDI SapI SfcI SmlI SphI StuI StyI TaqII TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BclI BfrBI BglI BmgBI BmrI BmtI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FseI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PsiI PspOMI PsrI PvuI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XmaI ZraI