Restriction map for
pF1KE2466
(Linear) (Six-base) MAPSORT of: KIEE2466.seq Check: 1808 from: 1 to: 2958
KIEE2466.orf
With 182 enzymes: SgfI *
August 29, 2014 10:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 541 1420 1858 2098 2104 2958
Size: 147 394 879 438 240 6 854
Fragments arranged by size:
879 854 438 394 240 147 6
AfeI AGC'GCT
Cuts at: 0 373 2958
Size: 373 2585
AflIII A'CryG_T
Cuts at: 0 772 1916 2456 2958
Size: 772 1144 540 502
Fragments arranged by size:
1144 772 540 502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2017 2958
Size: 2017 941
AlwNI CAG_nnn'CTG
Cuts at: 0 1163 2697 2829 2832 2958
Size: 1163 1534 132 3 126
Fragments arranged by size:
1534 1163 132 126 3
ApoI r'AATT_y
Cuts at: 0 1848 2958
Size: 1848 1110
AseI AT'TA_AT
Cuts at: 0 551 2958
Size: 551 2407
AvaI C'yCGr_G
Cuts at: 0 219 674 1905 2958
Size: 219 455 1231 1053
Fragments arranged by size:
1231 1053 455 219
BanII G_rGCy'C
Cuts at: 0 276 344 1177 2098 2162 2315 2958
Size: 276 68 833 921 64 153 643
Fragments arranged by size:
921 833 643 276 153 68 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 1047 1221 1233 2679 2958
Size: 1047 174 12 1446 279
Fragments arranged by size:
1446 1047 279 174 12
BclI T'GATC_A
Cuts at: 0 2195 2958
Size: 2195 763
BfrBI ATG'CAT
Cuts at: 0 1982 2958
Size: 1982 976
BglI GCCn_nnn'nGGC
Cuts at: 0 1255 1642 2659 2671 2958
Size: 1255 387 1017 12 287
Fragments arranged by size:
1255 1017 387 287 12
BglII A'GATC_T
Cuts at: 0 755 2579 2958
Size: 755 1824 379
Fragments arranged by size:
1824 755 379
BlpI GC'TnA_GC
Cuts at: 0 629 1176 1990 2958
Size: 629 547 814 968
Fragments arranged by size:
968 814 629 547
Bme1580I G_kGCm'C
Cuts at: 0 106 918 1138 2958
Size: 106 812 220 1820
Fragments arranged by size:
1820 812 220 106
BmgBI CAC'GTC
Cuts at: 0 773 2423 2958
Size: 773 1650 535
Fragments arranged by size:
1650 773 535
BmrI ACTGGGnnnn_n'
Cuts at: 0 2736 2958
Size: 2736 222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 36 2958
Size: 36 2922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 439 445 577 2176 2958
Size: 427 12 6 132 1599 782
Fragments arranged by size:
1599 782 427 132 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 333 2958
Size: 333 2625
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 1566 2958
Size: 550 1016 1392
Fragments arranged by size:
1392 1016 550
BsaAI yAC'GTr
Cuts at: 0 1872 1917 2958
Size: 1872 45 1041
Fragments arranged by size:
1872 1041 45
BsaBI GATnn'nnATC
Cuts at: 0 1024 2044 2958
Size: 1024 1020 914
Fragments arranged by size:
1024 1020 914
BsaHI Gr'CG_yC
Cuts at: 0 316 1607 1902 2291 2951 2958
Size: 316 1291 295 389 660 7
Fragments arranged by size:
1291 660 389 316 295 7
BsaWI w'CCGG_w
Cuts at: 0 227 2958
Size: 227 2731
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1546 1576 1609 1639 2488 2518 2620 2650
Size: 1546 30 33 30 849 30 102 30
Cuts at: 2650 2958
Size: 308
Fragments arranged by size:
1546 849 308 102 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 1354 1459 1644 1822 2125 2496 2824
Size: 400 954 105 185 178 303 371 328
Cuts at: 2824 2958
Size: 134
Fragments arranged by size:
954 400 371 328 303 185 178 134
105
BseYI C'CCAG_C
Cuts at: 0 1002 1093 1310 1539 2004 2922 2929 2958
Size: 1002 91 217 229 465 918 7 29
Fragments arranged by size:
1002 918 465 229 217 91 29 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 516 832 2596 2958
Size: 268 248 316 1764 362
Fragments arranged by size:
1764 362 316 268 248
BsiEI CG_ry'CG
Cuts at: 0 35 174 2958
Size: 35 139 2784
Fragments arranged by size:
2784 139 35
BsiHKAI G_wGCw'C
Cuts at: 0 593 635 742 1177 1277 2315 2786 2958
Size: 593 42 107 435 100 1038 471 172
Fragments arranged by size:
1038 593 471 435 172 107 100 42
Bsp1286I G_dGCh'C
Cuts at: 0 106 276 344 593 635 742 918 1138
Size: 106 170 68 249 42 107 176 220
Cuts at: 1138 1177 1277 2098 2162 2315 2786 2958
Size: 39 100 821 64 153 471 172
Fragments arranged by size:
821 471 249 220 176 172 170 153
107 106 100 68 64 42 39
BspHI T'CATG_A
Cuts at: 0 1574 2708 2958
Size: 1574 1134 250
Fragments arranged by size:
1574 1134 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 1162 2576 2958
Size: 98 1064 1414 382
Fragments arranged by size:
1414 1064 382 98
BsrBI CCG'CTC
Cuts at: 0 429 2856 2958
Size: 429 2427 102
Fragments arranged by size:
2427 429 102
BsrFI r'CCGG_y
Cuts at: 0 173 1430 1889 2322 2958
Size: 173 1257 459 433 636
Fragments arranged by size:
1257 636 459 433 173
BsrGI T'GTAC_A
Cuts at: 0 775 1010 1919 2459 2958
Size: 775 235 909 540 499
Fragments arranged by size:
909 775 540 499 235
BssSI C'ACGA_G
Cuts at: 0 358 804 2107 2958
Size: 358 446 1303 851
Fragments arranged by size:
1303 851 446 358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2829 2958
Size: 2829 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 954 1269 1386 2958
Size: 954 315 117 1572
Fragments arranged by size:
1572 954 315 117
BstYI r'GATC_y
Cuts at: 0 606 755 2279 2579 2765 2958
Size: 606 149 1524 300 186 193
Fragments arranged by size:
1524 606 300 193 186 149
BtgI C'CryG_G
Cuts at: 0 450 561 1076 1187 1223 1484 1628 2611
Size: 450 111 515 111 36 261 144 983
Cuts at: 2611 2943 2958
Size: 332 15
Fragments arranged by size:
983 515 450 332 261 144 111 111
36 15
BtsI GCAGTG_nn'
Cuts at: 0 844 1589 2958
Size: 844 745 1369
Fragments arranged by size:
1369 844 745
DraIII CAC_nnn'GTG
Cuts at: 0 1133 2958
Size: 1133 1825
DrdI GACnn_nn'nnGTC
Cuts at: 0 46 2958
Size: 46 2912
EaeI y'GGCC_r
Cuts at: 0 32 171 815 1631 1643 2958
Size: 32 139 644 816 12 1315
Fragments arranged by size:
1315 816 644 139 32 12
EagI C'GGCC_G
Cuts at: 0 32 171 2958
Size: 32 139 2787
Fragments arranged by size:
2787 139 32
EarI CTCTTCn'nnn_
Cuts at: 0 646 757 902 1213 1325 1331 1940 2116
Size: 646 111 145 311 112 6 609 176
Cuts at: 2116 2958
Size: 842
Fragments arranged by size:
842 646 609 311 176 145 112 111
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2272 2958
Size: 2272 686
EcoICRI GAG'CTC
Cuts at: 0 1175 2313 2958
Size: 1175 1138 645
Fragments arranged by size:
1175 1138 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 427 439 445 541 577 1420 1858
Size: 147 280 12 6 96 36 843 438
Cuts at: 1858 2098 2104 2176 2958
Size: 240 6 72 782
Fragments arranged by size:
843 782 438 280 240 147 96 72
36 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 1738 2958
Size: 406 1332 1220
Fragments arranged by size:
1332 1220 406
EcoO109I rG'GnC_Cy
Cuts at: 0 215 638 1314 1782 2958
Size: 215 423 676 468 1176
Fragments arranged by size:
1176 676 468 423 215
EcoRI G'AATT_C
Cuts at: 0 1848 2958
Size: 1848 1110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 732 2958
Size: 732 2226
HaeII r_GCGC'y
Cuts at: 0 375 1431 2958
Size: 375 1056 1527
Fragments arranged by size:
1527 1056 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 316 348 1109 1141 2958
Size: 250 32 34 32 761 32 1817
Fragments arranged by size:
1817 761 250 34 32 32 32
HincII GTy'rAC
Cuts at: 0 9 1451 2493 2958
Size: 9 1442 1042 465
Fragments arranged by size:
1442 1042 465 9
HindIII A'AGCT_T
Cuts at: 0 1800 2383 2958
Size: 1800 583 575
Fragments arranged by size:
1800 583 575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1697 1878 2597 2958
Size: 1697 181 719 361
Fragments arranged by size:
1697 719 361 181
MscI TGG'CCA
Cuts at: 0 817 1645 2958
Size: 817 828 1313
Fragments arranged by size:
1313 828 817
MslI CAynn'nnrTG
Cuts at: 0 196 362 551 1018 1102 1267 1573 1999
Size: 196 166 189 467 84 165 306 426
Cuts at: 1999 2191 2707 2958
Size: 192 516 251
Fragments arranged by size:
516 467 426 306 251 196 192 189
166 165 84
MspA1I CmG'CkG
Cuts at: 0 1310 2016 2133 2829 2958
Size: 1310 706 117 696 129
Fragments arranged by size:
1310 706 696 129 117
NaeI GCC'GGC
Cuts at: 0 175 1432 2958
Size: 175 1257 1526
Fragments arranged by size:
1526 1257 175
NcoI C'CATG_G
Cuts at: 0 1076 1484 1628 2958
Size: 1076 408 144 1330
Fragments arranged by size:
1330 1076 408 144
NgoMIV G'CCGG_C
Cuts at: 0 173 1430 2958
Size: 173 1257 1528
Fragments arranged by size:
1528 1257 173
NsiI A_TGCA'T
Cuts at: 0 1984 2958
Size: 1984 974
NspI r_CATG'y
Cuts at: 0 2460 2958
Size: 2460 498
PciI A'CATG_T
Cuts at: 0 2456 2958
Size: 2456 502
PflMI CCAn_nnn'nTGG
Cuts at: 0 2697 2958
Size: 2697 261
PfoI T'CCnGG_A
Cuts at: 0 404 1733 2735 2958
Size: 404 1329 1002 223
Fragments arranged by size:
1329 1002 404 223
PpuMI rG'GwC_Cy
Cuts at: 0 1314 1782 2958
Size: 1314 468 1176
Fragments arranged by size:
1314 1176 468
PshAI GACnn'nnGTC
Cuts at: 0 235 2958
Size: 235 2723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 759 791 2958
Size: 759 32 2167
Fragments arranged by size:
2167 759 32
PstI C_TGCA'G
Cuts at: 0 1159 2587 2958
Size: 1159 1428 371
Fragments arranged by size:
1428 1159 371
PvuII CAG'CTG
Cuts at: 0 2016 2829 2958
Size: 2016 813 129
Fragments arranged by size:
2016 813 129
SacI G_AGCT'C
Cuts at: 0 1177 2315 2958
Size: 1177 1138 643
Fragments arranged by size:
1177 1138 643
ScaI AGT'ACT
Cuts at: 0 19 2958
Size: 19 2939
SexAI A'CCwGG_T
Cuts at: 0 953 2696 2958
Size: 953 1743 262
Fragments arranged by size:
1743 953 262
SfcI C'TryA_G
Cuts at: 0 1155 1617 2583 2958
Size: 1155 462 966 375
Fragments arranged by size:
1155 966 462 375
SmaI CCC'GGG
Cuts at: 0 676 1907 2958
Size: 676 1231 1051
Fragments arranged by size:
1231 1051 676
SrfI GCCC'GGGC
Cuts at: 0 1907 2958
Size: 1907 1051
StyI C'CwwG_G
Cuts at: 0 1076 1484 1628 1640 2639 2747 2958
Size: 1076 408 144 12 999 108 211
Fragments arranged by size:
1076 999 408 211 144 108 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 2958
Size: 1030 1928
TatI w'GTAC_w
Cuts at: 0 17 158 775 788 989 1010 1919 2183
Size: 17 141 617 13 201 21 909 264
Cuts at: 2183 2459 2958
Size: 276 499
Fragments arranged by size:
909 617 499 276 264 201 141 21
17 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2488 2520 2958
Size: 2488 32 438
Fragments arranged by size:
2488 438 32
Tth111I GACn'n_nGTC
Cuts at: 0 955 2728 2958
Size: 955 1773 230
Fragments arranged by size:
1773 955 230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1869 2553 2958
Size: 792 1077 684 405
Fragments arranged by size:
1077 792 684 405
XmaI C'CCGG_G
Cuts at: 0 674 1905 2958
Size: 674 1231 1053
Fragments arranged by size:
1231 1053 674
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApoI AseI AvaI
BanII BbsI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PpuMI PshAI PsrI PstI PvuII SacI ScaI SexAI
SfcI SmaI SrfI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BmtI BpuEI
BsiWI BsmI BsmBI BspEI BsrDI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI
XmnI ZraI