Restriction map for
pF1KE2469
(Linear) (Six-base) MAPSORT of: KIEE2469.seq Check: 8751 from: 1 to: 4746
KIEE2469.orf
With 182 enzymes: SgfI *
May 14, 2013 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2130 4746
Size: 2130 2616
AatII G_ACGT'C
Cuts at: 0 998 4746
Size: 998 3748
AccI GT'mk_AC
Cuts at: 0 365 4746
Size: 365 4381
Acc65I G'GTAC_C
Cuts at: 0 2734 4746
Size: 2734 2012
AclI AA'CG_TT
Cuts at: 0 2546 4746
Size: 2546 2200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1546 3292 3633 4093 4105 4278 4399
Size: 1507 39 1746 341 460 12 173 121
Cuts at: 4399 4675 4746
Size: 276 71
Fragments arranged by size:
1746 1507 460 341 276 173 121 71
39 12
AflIII A'CryG_T
Cuts at: 0 4190 4746
Size: 4190 556
AhdI GACnn_n'nnGTC
Cuts at: 0 2955 4746
Size: 2955 1791
AleI CACnn'nnGTG
Cuts at: 0 358 1561 2975 3484 4746
Size: 358 1203 1414 509 1262
Fragments arranged by size:
1414 1262 1203 509 358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1537 2993 4112 4746
Size: 1537 1456 1119 634
Fragments arranged by size:
1537 1456 1119 634
AlwNI CAG_nnn'CTG
Cuts at: 0 1995 2206 2679 3149 3573 4047 4746
Size: 1995 211 473 470 424 474 699
Fragments arranged by size:
1995 699 474 473 470 424 211
ApaI G_GGCC'C
Cuts at: 0 777 1900 4746
Size: 777 1123 2846
Fragments arranged by size:
2846 1123 777
ApaLI G'TGCA_C
Cuts at: 0 172 3168 4618 4746
Size: 172 2996 1450 128
Fragments arranged by size:
2996 1450 172 128
ApoI r'AATT_y
Cuts at: 0 4440 4746
Size: 4440 306
AvaI C'yCGr_G
Cuts at: 0 205 619 851 1021 1955 2111 2522 2883
Size: 205 414 232 170 934 156 411 361
Cuts at: 2883 4478 4746
Size: 1595 268
Fragments arranged by size:
1595 934 414 411 361 268 232 205
170 156
BamHI G'GATC_C
Cuts at: 0 224 4746
Size: 224 4522
BanI G'GyrC_C
Cuts at: 0 99 682 847 890 1920 2716 2734 3990
Size: 99 583 165 43 1030 796 18 1256
Cuts at: 3990 4300 4663 4746
Size: 310 363 83
Fragments arranged by size:
1256 1030 796 583 363 310 165 99
83 43 18
BanII G_rGCy'C
Cuts at: 0 166 777 1886 1900 2529 3827 3920 3953
Size: 166 611 1109 14 629 1298 93 33
Cuts at: 3953 3986 4746
Size: 33 760
Fragments arranged by size:
1298 1109 760 629 611 166 93 33
33 14
BbeI G_GCGC'C
Cuts at: 0 894 4746
Size: 894 3852
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 990 2202 2544 2791 3042 4501 4710
Size: 102 888 1212 342 247 251 1459 209
Cuts at: 4710 4746
Size: 36
Fragments arranged by size:
1459 1212 888 342 251 247 209 102
36
BbvCI CC'TCA_GC
Cuts at: 0 901 3157 4746
Size: 901 2256 1589
Fragments arranged by size:
2256 1589 901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4209 4243 4746
Size: 4209 34 503
Fragments arranged by size:
4209 503 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2116 4746
Size: 2116 2630
BglI GCCn_nnn'nGGC
Cuts at: 0 334 772 1979 3842 4142 4746
Size: 334 438 1207 1863 300 604
Fragments arranged by size:
1863 1207 604 438 334 300
BglII A'GATC_T
Cuts at: 0 1247 4746
Size: 1247 3499
BlpI GC'TnA_GC
Cuts at: 0 1828 4699 4746
Size: 1828 2871 47
Fragments arranged by size:
2871 1828 47
Bme1580I G_kGCm'C
Cuts at: 0 176 212 777 841 852 1306 1875 1900
Size: 176 36 565 64 11 454 569 25
Cuts at: 1900 1925 3172 4205 4622 4746
Size: 25 1247 1033 417 124
Fragments arranged by size:
1247 1033 569 565 454 417 176 124
64 36 25 25 11
BmgBI CAC'GTC
Cuts at: 0 108 3245 4092 4746
Size: 108 3137 847 654
Fragments arranged by size:
3137 847 654 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 1997 2188 4746
Size: 1997 191 2558
Fragments arranged by size:
2558 1997 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 4746
Size: 48 4698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 2235 2836 3439 3904 4746
Size: 1486 749 601 603 465 842
Fragments arranged by size:
1486 842 749 603 601 465
Bpu10I CC'TnA_GC
Cuts at: 0 901 3157 3427 4048 4746
Size: 901 2256 270 621 698
Fragments arranged by size:
2256 901 698 621 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1179 2641 2725 3049 4693 4746
Size: 827 352 1462 84 324 1644 53
Fragments arranged by size:
1644 1462 827 352 324 84 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 2202 2447 3334 3876 4746
Size: 2202 245 887 542 870
Fragments arranged by size:
2202 887 870 542 245
BsaAI yAC'GTr
Cuts at: 0 2060 4746
Size: 2060 2686
BsaBI GATnn'nnATC
Cuts at: 0 2585 3375 4036 4126 4746
Size: 2585 790 661 90 620
Fragments arranged by size:
2585 790 661 620 90
BsaHI Gr'CG_yC
Cuts at: 0 812 891 995 3553 4746
Size: 812 79 104 2558 1193
Fragments arranged by size:
2558 1193 812 104 79
BsaWI w'CCGG_w
Cuts at: 0 3308 4746
Size: 3308 1438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1379 1409 3001 3031 3679 3709 4051 4081
Size: 1379 30 1592 30 648 30 342 30
Cuts at: 4081 4746
Size: 665
Fragments arranged by size:
1592 1379 665 648 342 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 591 1178 1342 1876 2368 2929 2938
Size: 538 53 587 164 534 492 561 9
Cuts at: 2938 2956 4746
Size: 18 1790
Fragments arranged by size:
1790 587 561 538 534 492 164 53
18 9
BseYI C'CCAG_C
Cuts at: 0 933 1451 1899 2254 3587 3924 4373 4746
Size: 933 518 448 355 1333 337 449 373
Fragments arranged by size:
1333 933 518 449 448 373 355 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1868 2027 2953 3274 3799 4228 4655
Size: 295 1573 159 926 321 525 429 427
Cuts at: 4655 4746
Size: 91
Fragments arranged by size:
1573 926 525 429 427 321 295 159
91
BsiEI CG_ry'CG
Cuts at: 0 38 2279 4137 4746
Size: 38 2241 1858 609
Fragments arranged by size:
2241 1858 609 38
BsiHKAI G_wGCw'C
Cuts at: 0 166 176 1462 2780 3172 3212 3782 3827
Size: 166 10 1286 1318 392 40 570 45
Cuts at: 3827 3920 4339 4622 4746
Size: 93 419 283 124
Fragments arranged by size:
1318 1286 570 419 392 283 166 124
93 45 40 10
BsmI GAATG_Cn'
Cuts at: 0 2767 4746
Size: 2767 1979
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1561 4746
Size: 1561 3185
Bsp1286I G_dGCh'C
Cuts at: 0 166 176 212 777 841 852 1306 1462
Size: 166 10 36 565 64 11 454 156
Cuts at: 1462 1875 1886 1900 1925 2529 2780 3172 3212
Size: 413 11 14 25 604 251 392 40
Cuts at: 3212 3782 3827 3920 3953 3986 4205 4339 4622
Size: 570 45 93 33 33 219 134 283
Cuts at: 4622 4746
Size: 124
Fragments arranged by size:
604 570 565 454 413 392 283 251
219 166 156 134 124 93 64 45
40 36 33 33 25 14 11 11
10
BspHI T'CATG_A
Cuts at: 0 1634 2792 4746
Size: 1634 1158 1954
Fragments arranged by size:
1954 1634 1158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 635 938 2130 2687 3572 4746
Size: 224 411 303 1192 557 885 1174
Fragments arranged by size:
1192 1174 885 557 411 303 224
BsrBI CCG'CTC
Cuts at: 0 1482 2265 3434 4746
Size: 1482 783 1169 1312
Fragments arranged by size:
1482 1312 1169 783
BsrDI GCAATG_nn'
Cuts at: 0 1652 2699 3704 4746
Size: 1652 1047 1005 1042
Fragments arranged by size:
1652 1047 1042 1005
BsrFI r'CCGG_y
Cuts at: 0 1978 2611 3022 4077 4141 4746
Size: 1978 633 411 1055 64 605
Fragments arranged by size:
1978 1055 633 605 411 64
BsrGI T'GTAC_A
Cuts at: 0 317 4746
Size: 317 4429
BssSI C'ACGA_G
Cuts at: 0 550 2870 3493 4746
Size: 550 2320 623 1253
Fragments arranged by size:
2320 1253 623 550
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 4251 4617 4746
Size: 216 4035 366 129
Fragments arranged by size:
4035 366 216 129
BstBI TT'CG_AA
Cuts at: 0 4196 4746
Size: 4196 550
BstEII G'GTnAC_C
Cuts at: 0 291 3792 4599 4746
Size: 291 3501 807 147
Fragments arranged by size:
3501 807 291 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1313 4013 4539 4746
Size: 1313 2700 526 207
Fragments arranged by size:
2700 1313 526 207
BstYI r'GATC_y
Cuts at: 0 224 1247 1853 2984 4031 4037 4127 4746
Size: 224 1023 606 1131 1047 6 90 619
Fragments arranged by size:
1131 1047 1023 619 606 224 90 6
Bsu36I CC'TnA_GG
Cuts at: 0 1597 1892 1927 4746
Size: 1597 295 35 2819
Fragments arranged by size:
2819 1597 295 35
BtgI C'CryG_G
Cuts at: 0 359 1223 1509 1721 2306 2329 2448 2989
Size: 359 864 286 212 585 23 119 541
Cuts at: 2989 3194 3945 4523 4544 4746
Size: 205 751 578 21 202
Fragments arranged by size:
864 751 585 578 541 359 286 212
205 202 119 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 747 2030 3768 3980 4089 4212 4590 4746
Size: 747 1283 1738 212 109 123 378 156
Fragments arranged by size:
1738 1283 747 378 212 156 123 109
BtsI GCAGTG_nn'
Cuts at: 0 1873 1984 4152 4396 4746
Size: 1873 111 2168 244 350
Fragments arranged by size:
2168 1873 350 244 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3333 3368 4746
Size: 3333 35 1378
Fragments arranged by size:
3333 1378 35
DraIII CAC_nnn'GTG
Cuts at: 0 4352 4746
Size: 4352 394
DrdI GACnn_nn'nnGTC
Cuts at: 0 58 3184 4746
Size: 58 3126 1562
Fragments arranged by size:
3126 1562 58
EaeI y'GGCC_r
Cuts at: 0 35 923 1136 1430 1643 3083 3101 3278
Size: 35 888 213 294 213 1440 18 177
Cuts at: 3278 3461 3512 4015 4134 4541 4746
Size: 183 51 503 119 407 205
Fragments arranged by size:
1440 888 503 407 294 213 213 205
183 177 119 51 35 18
EagI C'GGCC_G
Cuts at: 0 35 4134 4746
Size: 35 4099 612
Fragments arranged by size:
4099 612 35
EarI CTCTTCn'nnn_
Cuts at: 0 124 145 250 1750 2290 2906 2915 3721
Size: 124 21 105 1500 540 616 9 806
Cuts at: 3721 4297 4746
Size: 576 449
Fragments arranged by size:
1500 806 616 576 540 449 124 105
21 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3009 4746
Size: 3009 1737
EcoICRI GAG'CTC
Cuts at: 0 164 3825 3918 4746
Size: 164 3661 93 828
Fragments arranged by size:
3661 828 164 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 1507 1546 2235 2836 3292 3439 3633
Size: 1486 21 39 689 601 456 147 194
Cuts at: 3633 3904 4093 4105 4278 4399 4675 4746
Size: 271 189 12 173 121 276 71
Fragments arranged by size:
1486 689 601 456 276 271 194 189
173 147 121 71 39 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 553 885 982 2899 3160 4488 4561 4746
Size: 553 332 97 1917 261 1328 73 185
Fragments arranged by size:
1917 1328 553 332 261 185 97 73
EcoO109I rG'GnC_Cy
Cuts at: 0 60 624 773 774 1601 1896 1971 2250
Size: 60 564 149 1 827 295 75 279
Cuts at: 2250 2302 2862 3561 4281 4359 4746
Size: 52 560 699 720 78 387
Fragments arranged by size:
827 720 699 564 560 387 295 279
149 78 75 60 52 1
EcoRV GAT'ATC
Cuts at: 0 2289 2583 4746
Size: 2289 294 2163
Fragments arranged by size:
2289 2163 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1069 4176 4746
Size: 1069 3107 570
Fragments arranged by size:
3107 1069 570
HaeII r_GCGC'y
Cuts at: 0 894 3122 4746
Size: 894 2228 1624
Fragments arranged by size:
2228 1624 894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1271 1379 1411 1784 1816 1825 1857
Size: 1239 32 108 32 373 32 9 32
Cuts at: 1857 2554 2586 2941 2973 4106 4138 4746
Size: 697 32 355 32 1133 32 608
Fragments arranged by size:
1239 1133 697 608 373 355 108 32
32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 2500 4746
Size: 2500 2246
HindIII A'AGCT_T
Cuts at: 0 3676 4746
Size: 3676 1070
KasI G'GCGC_C
Cuts at: 0 890 4746
Size: 890 3856
KpnI G_GTAC'C
Cuts at: 0 2738 4746
Size: 2738 2008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1238 2114 3722 3830 3899 4746
Size: 413 825 876 1608 108 69 847
Fragments arranged by size:
1608 876 847 825 413 108 69
MscI TGG'CCA
Cuts at: 0 1138 1432 3085 3103 3280 3514 4017 4543
Size: 1138 294 1653 18 177 234 503 526
Cuts at: 4543 4746
Size: 203
Fragments arranged by size:
1653 1138 526 503 294 234 203 177
18
MslI CAynn'nnrTG
Cuts at: 0 358 571 766 1561 1633 2591 2704 2975
Size: 358 213 195 795 72 958 113 271
Cuts at: 2975 3484 4094 4537 4746
Size: 509 610 443 209
Fragments arranged by size:
958 795 610 509 443 358 271 213
209 195 113 72
MspA1I CmG'CkG
Cuts at: 0 1393 1455 1511 1660 1767 2121 2932 3060
Size: 1393 62 56 149 107 354 811 128
Cuts at: 3060 3573 3727 3790 3924 4242 4298 4377 4464
Size: 513 154 63 134 318 56 79 87
Cuts at: 4464 4746
Size: 282
Fragments arranged by size:
1393 811 513 354 318 282 154 149
134 128 107 87 79 63 62 56
56
NaeI GCC'GGC
Cuts at: 0 2613 3024 4746
Size: 2613 411 1722
Fragments arranged by size:
2613 1722 411
NarI GG'CG_CC
Cuts at: 0 891 4746
Size: 891 3855
NcoI C'CATG_G
Cuts at: 0 1223 2329 2448 2989 3194 3945 4746
Size: 1223 1106 119 541 205 751 801
Fragments arranged by size:
1223 1106 801 751 541 205 119
NgoMIV G'CCGG_C
Cuts at: 0 2611 3022 4746
Size: 2611 411 1724
Fragments arranged by size:
2611 1724 411
NotI GC'GGCC_GC
Cuts at: 0 4134 4746
Size: 4134 612
NspI r_CATG'y
Cuts at: 0 324 3773 4746
Size: 324 3449 973
Fragments arranged by size:
3449 973 324
PasI CC'CwG_GG
Cuts at: 0 2859 4746
Size: 2859 1887
PflMI CCAn_nnn'nTGG
Cuts at: 0 1755 2043 2814 3737 4566 4746
Size: 1755 288 771 923 829 180
Fragments arranged by size:
1755 923 829 771 288 180
PfoI T'CCnGG_A
Cuts at: 0 2630 4746
Size: 2630 2116
PmlI CAC'GTG
Cuts at: 0 2060 4746
Size: 2060 2686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3649 3681 4746
Size: 3649 32 1065
Fragments arranged by size:
3649 1065 32
PpuMI rG'GwC_Cy
Cuts at: 0 60 624 2250 3561 4281 4746
Size: 60 564 1626 1311 720 465
Fragments arranged by size:
1626 1311 720 564 465 60
PshAI GACnn'nnGTC
Cuts at: 0 2650 4746
Size: 2650 2096
PspOMI G'GGCC_C
Cuts at: 0 773 1896 4746
Size: 773 1123 2850
Fragments arranged by size:
2850 1123 773
PspXI vC'TCGA_Gb
Cuts at: 0 2883 4746
Size: 2883 1863
PstI C_TGCA'G
Cuts at: 0 656 1991 2426 2903 3141 3217 3569 4280
Size: 656 1335 435 477 238 76 352 711
Cuts at: 4280 4746
Size: 466
Fragments arranged by size:
1335 711 656 477 466 435 352 238
76
PvuII CAG'CTG
Cuts at: 0 1393 1660 2121 2932 3060 3573 3727 3924
Size: 1393 267 461 811 128 513 154 197
Cuts at: 3924 4377 4464 4746
Size: 453 87 282
Fragments arranged by size:
1393 811 513 461 453 282 267 197
154 128 87
SacI G_AGCT'C
Cuts at: 0 166 3827 3920 4746
Size: 166 3661 93 826
Fragments arranged by size:
3661 826 166 93
SacII CC_GC'GG
Cuts at: 0 1512 4746
Size: 1512 3234
SapI GCTCTTCn'nnn_
Cuts at: 0 250 4746
Size: 250 4496
SbfI CC_TGCA'GG
Cuts at: 0 4280 4746
Size: 4280 466
ScaI AGT'ACT
Cuts at: 0 1423 3541 4746
Size: 1423 2118 1205
Fragments arranged by size:
2118 1423 1205
SexAI A'CCwGG_T
Cuts at: 0 4640 4746
Size: 4640 106
SfcI C'TryA_G
Cuts at: 0 652 1987 2422 2671 2899 3137 3213 3565
Size: 652 1335 435 249 228 238 76 352
Cuts at: 3565 4276 4746
Size: 711 470
Fragments arranged by size:
1335 711 652 470 435 352 249 238
228 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1979 3842 4746
Size: 1979 1863 904
Fragments arranged by size:
1979 1863 904
SfoI GGC'GCC
Cuts at: 0 892 4746
Size: 892 3854
SmaI CCC'GGG
Cuts at: 0 207 853 1957 4480 4746
Size: 207 646 1104 2523 266
Fragments arranged by size:
2523 1104 646 266 207
SmlI C'TyrA_G
Cuts at: 0 842 1158 2620 2740 2883 3064 4672 4746
Size: 842 316 1462 120 143 181 1608 74
Fragments arranged by size:
1608 1462 842 316 181 143 120 74
SrfI GCCC'GGGC
Cuts at: 0 853 4480 4746
Size: 853 3627 266
Fragments arranged by size:
3627 853 266
StuI AGG'CCT
Cuts at: 0 805 4409 4746
Size: 805 3604 337
Fragments arranged by size:
3604 805 337
StyI C'CwwG_G
Cuts at: 0 167 978 1223 2329 2448 2989 3194 3351
Size: 167 811 245 1106 119 541 205 157
Cuts at: 3351 3945 4023 4746
Size: 594 78 723
Fragments arranged by size:
1106 811 723 594 541 245 205 167
157 119 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 1330 2293 3127 3601 4746
Size: 310 1020 963 834 474 1145
Fragments arranged by size:
1145 1020 963 834 474 310
TatI w'GTAC_w
Cuts at: 0 317 1326 1421 3539 3758 4746
Size: 317 1009 95 2118 219 988
Fragments arranged by size:
2118 1009 988 317 219 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1754 1786 3394 3426 3473 3505 3576 3608
Size: 1754 32 1608 32 47 32 71 32
Cuts at: 3608 4746
Size: 1138
Fragments arranged by size:
1754 1608 1138 71 47 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4285 4714 4746
Size: 4285 429 32
Fragments arranged by size:
4285 429 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1221 2464 3738 4746
Size: 1221 1243 1274 1008
Fragments arranged by size:
1274 1243 1221 1008
XhoI C'TCGA_G
Cuts at: 0 2883 4746
Size: 2883 1863
XmaI C'CCGG_G
Cuts at: 0 205 851 1955 4478 4746
Size: 205 646 1104 2523 268
Fragments arranged by size:
2523 1104 646 268 205
XmnI GAAnn'nnTTC
Cuts at: 0 703 1549 3541 4746
Size: 703 846 1992 1205
Fragments arranged by size:
1992 1205 846 703
ZraI GAC'GTC
Cuts at: 0 996 4746
Size: 996 3750
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AloI AscI AseI AsiSI AvrII
BaeI BclI BfrBI BmtI BsiWI BspEI BssHII BstZ17I
ClaI DraI EcoRI FseI FspI FspAI HpaI MfeI
MluI NdeI NheI NruI NsiI PacI PciI PmeI
PsiI PsrI PvuI RsrII SalI SanDI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI XbaI