Restriction map for
pF1KE2473
(Linear) (Six-base) MAPSORT of: KIEE2473.seq Check: 1220 from: 1 to: 2526
KIEE2473.orf
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1064 2526
Size: 1064 1462
AccI GT'mk_AC
Cuts at: 0 709 2526
Size: 709 1817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1197 1773 2159 2194 2516 2526
Size: 229 968 576 386 35 322 10
Fragments arranged by size:
968 576 386 322 229 35 10
AfeI AGC'GCT
Cuts at: 0 1473 2267 2526
Size: 1473 794 259
Fragments arranged by size:
1473 794 259
AflIII A'CryG_T
Cuts at: 0 1688 1823 2459 2526
Size: 1688 135 636 67
Fragments arranged by size:
1688 636 135 67
AleI CACnn'nnGTG
Cuts at: 0 91 516 2526
Size: 91 425 2010
Fragments arranged by size:
2010 425 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1912 2260 2526
Size: 1912 348 266
Fragments arranged by size:
1912 348 266
AlwNI CAG_nnn'CTG
Cuts at: 0 405 1506 2526
Size: 405 1101 1020
Fragments arranged by size:
1101 1020 405
ApaI G_GGCC'C
Cuts at: 0 38 303 618 1745 1870 2526
Size: 38 265 315 1127 125 656
Fragments arranged by size:
1127 656 315 265 125 38
ApaLI G'TGCA_C
Cuts at: 0 1783 2526
Size: 1783 743
AvaI C'yCGr_G
Cuts at: 0 293 764 1346 1519 2438 2526
Size: 293 471 582 173 919 88
Fragments arranged by size:
919 582 471 293 173 88
BamHI G'GATC_C
Cuts at: 0 1001 2526
Size: 1001 1525
BanI G'GyrC_C
Cuts at: 0 352 843 888 1079 1088 1600 1642 2526
Size: 352 491 45 191 9 512 42 884
Fragments arranged by size:
884 512 491 352 191 45 42 9
BanII G_rGCy'C
Cuts at: 0 38 303 618 812 1067 1520 1526 1745
Size: 38 265 315 194 255 453 6 219
Cuts at: 1745 1870 2526
Size: 125 656
Fragments arranged by size:
656 453 315 265 255 219 194 125
38 6
BbeI G_GCGC'C
Cuts at: 0 356 1092 2526
Size: 356 736 1434
Fragments arranged by size:
1434 736 356
BbsI GAAGACnn'nnnn_
Cuts at: 0 921 2504 2526
Size: 921 1583 22
Fragments arranged by size:
1583 921 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2316 2350 2526
Size: 2316 34 176
Fragments arranged by size:
2316 176 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2265 2526
Size: 2265 261
BglI GCCn_nnn'nGGC
Cuts at: 0 271 275 284 298 2128 2296 2526
Size: 271 4 9 14 1830 168 230
Fragments arranged by size:
1830 271 230 168 14 9 4
BlpI GC'TnA_GC
Cuts at: 0 1150 2179 2526
Size: 1150 1029 347
Fragments arranged by size:
1150 1029 347
Bme1580I G_kGCm'C
Cuts at: 0 38 98 289 303 618 831 1129 1353
Size: 38 60 191 14 315 213 298 224
Cuts at: 1353 1745 1787 1870 2009 2526
Size: 392 42 83 139 517
Fragments arranged by size:
517 392 315 298 224 213 191 139
83 60 42 38 14
BmgBI CAC'GTC
Cuts at: 0 1022 1824 2526
Size: 1022 802 702
Fragments arranged by size:
1022 802 702
BmrI ACTGGGnnnn_n'
Cuts at: 0 627 2526
Size: 627 1899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 794 2526
Size: 794 1732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 608 928 1754 2526
Size: 358 250 320 826 772
Fragments arranged by size:
826 772 358 320 250
Bpu10I CC'TnA_GC
Cuts at: 0 2099 2526
Size: 2099 427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 2209 2526
Size: 919 1290 317
Fragments arranged by size:
1290 919 317
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 588 613 1994 2526
Size: 451 137 25 1381 532
Fragments arranged by size:
1381 532 451 137 25
BsaAI yAC'GTr
Cuts at: 0 219 2526
Size: 219 2307
BsaHI Gr'CG_yC
Cuts at: 0 153 353 1089 2526
Size: 153 200 736 1437
Fragments arranged by size:
1437 736 200 153
BsaWI w'CCGG_w
Cuts at: 0 1985 2526
Size: 1985 541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1450 1480 1675 1705 2526
Size: 1450 30 195 30 821
Fragments arranged by size:
1450 821 195 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 1516 1723 2526
Size: 445 1071 207 803
Fragments arranged by size:
1071 803 445 207
BseYI C'CCAG_C
Cuts at: 0 2071 2526
Size: 2071 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1042 2526
Size: 1030 12 1484
Fragments arranged by size:
1484 1030 12
BsiEI CG_ry'CG
Cuts at: 0 2061 2526
Size: 2061 465
BsiHKAI G_wGCw'C
Cuts at: 0 797 1067 1595 1787 1891 2526
Size: 797 270 528 192 104 635
Fragments arranged by size:
797 635 528 270 192 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1028 1375 2526
Size: 1028 347 1151
Fragments arranged by size:
1151 1028 347
Bsp1286I G_dGCh'C
Cuts at: 0 38 98 289 303 618 797 812 831
Size: 38 60 191 14 315 179 15 19
Cuts at: 831 1067 1129 1353 1520 1526 1595 1745 1787
Size: 236 62 224 167 6 69 150 42
Cuts at: 1787 1870 1891 2009 2526
Size: 83 21 118 517
Fragments arranged by size:
517 315 236 224 191 179 167 150
118 83 69 62 60 42 38 21
19 15 14 6
BspHI T'CATG_A
Cuts at: 0 654 2526
Size: 654 1872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 537 639 992 1064 2526
Size: 537 102 353 72 1462
Fragments arranged by size:
1462 537 353 102 72
BsrBI CCG'CTC
Cuts at: 0 162 384 2166 2183 2526
Size: 162 222 1782 17 343
Fragments arranged by size:
1782 343 222 162 17
BsrDI GCAATG_nn'
Cuts at: 0 416 643 2207 2526
Size: 416 227 1564 319
Fragments arranged by size:
1564 416 319 227
BsrFI r'CCGG_y
Cuts at: 0 196 265 349 1531 2526
Size: 196 69 84 1182 995
Fragments arranged by size:
1182 995 196 84 69
BsrGI T'GTAC_A
Cuts at: 0 1619 2526
Size: 1619 907
BssSI C'ACGA_G
Cuts at: 0 91 1060 1352 2422 2526
Size: 91 969 292 1070 104
Fragments arranged by size:
1070 969 292 104 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 20 2526
Size: 20 2506
BstEII G'GTnAC_C
Cuts at: 0 1968 2526
Size: 1968 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 518 952 2340 2526
Size: 518 434 1388 186
Fragments arranged by size:
1388 518 434 186
BstYI r'GATC_y
Cuts at: 0 1001 2378 2526
Size: 1001 1377 148
Fragments arranged by size:
1377 1001 148
BtgI C'CryG_G
Cuts at: 0 38 2516 2526
Size: 38 2478 10
Fragments arranged by size:
2478 38 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2022 2526
Size: 2022 504
BtsI GCAGTG_nn'
Cuts at: 0 287 304 1069 1364 2526
Size: 287 17 765 295 1162
Fragments arranged by size:
1162 765 295 287 17
DraIII CAC_nnn'GTG
Cuts at: 0 1298 2526
Size: 1298 1228
EaeI y'GGCC_r
Cuts at: 0 236 263 276 347 1184 2058 2306 2526
Size: 236 27 13 71 837 874 248 220
Fragments arranged by size:
874 837 248 236 220 71 27 13
EagI C'GGCC_G
Cuts at: 0 2058 2526
Size: 2058 468
EarI CTCTTCn'nnn_
Cuts at: 0 146 653 1879 2526
Size: 146 507 1226 647
Fragments arranged by size:
1226 647 507 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 727 2526
Size: 727 1799
EcoICRI GAG'CTC
Cuts at: 0 1065 2526
Size: 1065 1461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 358 608 928 1197 1754 1773 2159
Size: 229 129 250 320 269 557 19 386
Cuts at: 2159 2194 2516 2526
Size: 35 322 10
Fragments arranged by size:
557 386 322 320 269 250 229 129
35 19 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1650 1736 2526
Size: 1650 86 790
Fragments arranged by size:
1650 790 86
EcoO109I rG'GnC_Cy
Cuts at: 0 34 299 300 615 1725 1741 1742 2526
Size: 34 265 1 315 1110 16 1 784
Fragments arranged by size:
1110 784 315 265 34 16 1 1
FseI GG_CCGG'CC
Cuts at: 0 200 269 2526
Size: 200 69 2257
Fragments arranged by size:
2257 200 69
FspI TGC'GCA
Cuts at: 0 988 1548 2526
Size: 988 560 978
Fragments arranged by size:
988 978 560
HaeII r_GCGC'y
Cuts at: 0 256 356 1092 1475 2269 2526
Size: 256 100 736 383 794 257
Fragments arranged by size:
794 736 383 257 256 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 1104 1136 1421 1450 1453 1482
Size: 169 32 903 32 285 29 3 29
Cuts at: 1482 1912 1944 2245 2277 2526
Size: 430 32 301 32 249
Fragments arranged by size:
903 430 301 285 249 169 32 32
32 32 29 29 3
KasI G'GCGC_C
Cuts at: 0 352 1088 2526
Size: 352 736 1438
Fragments arranged by size:
1438 736 352
MfeI C'AATT_G
Cuts at: 0 1632 2526
Size: 1632 894
MscI TGG'CCA
Cuts at: 0 238 2308 2526
Size: 238 2070 218
Fragments arranged by size:
2070 238 218
MslI CAynn'nnrTG
Cuts at: 0 91 516 1678 1837 2338 2526
Size: 91 425 1162 159 501 188
Fragments arranged by size:
1162 501 425 188 159 91
MspA1I CmG'CkG
Cuts at: 0 169 228 478 786 1617 2071 2469 2526
Size: 169 59 250 308 831 454 398 57
Fragments arranged by size:
831 454 398 308 250 169 59 57
NaeI GCC'GGC
Cuts at: 0 198 267 351 1533 2526
Size: 198 69 84 1182 993
Fragments arranged by size:
1182 993 198 84 69
NarI GG'CG_CC
Cuts at: 0 353 1089 2526
Size: 353 736 1437
Fragments arranged by size:
1437 736 353
NcoI C'CATG_G
Cuts at: 0 2516 2526
Size: 2516 10
NgoMIV G'CCGG_C
Cuts at: 0 196 265 349 1531 2526
Size: 196 69 84 1182 995
Fragments arranged by size:
1182 995 196 84 69
NspI r_CATG'y
Cuts at: 0 1221 1626 1692 2463 2526
Size: 1221 405 66 771 63
Fragments arranged by size:
1221 771 405 66 63
PciI A'CATG_T
Cuts at: 0 1688 2459 2526
Size: 1688 771 67
Fragments arranged by size:
1688 771 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 1506 2526
Size: 672 834 1020
Fragments arranged by size:
1020 834 672
PfoI T'CCnGG_A
Cuts at: 0 1731 2526
Size: 1731 795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 815 847 2526
Size: 815 32 1679
Fragments arranged by size:
1679 815 32
PpuMI rG'GwC_Cy
Cuts at: 0 1725 2526
Size: 1725 801
PspOMI G'GGCC_C
Cuts at: 0 34 299 614 1741 1866 2526
Size: 34 265 315 1127 125 660
Fragments arranged by size:
1127 660 315 265 125 34
PspXI vC'TCGA_Gb
Cuts at: 0 764 2526
Size: 764 1762
PstI C_TGCA'G
Cuts at: 0 151 227 785 1820 2109 2474 2526
Size: 151 76 558 1035 289 365 52
Fragments arranged by size:
1035 558 365 289 151 76 52
PvuII CAG'CTG
Cuts at: 0 228 478 786 1617 2071 2469 2526
Size: 228 250 308 831 454 398 57
Fragments arranged by size:
831 454 398 308 250 228 57
SacI G_AGCT'C
Cuts at: 0 1067 2526
Size: 1067 1459
SapI GCTCTTCn'nnn_
Cuts at: 0 146 1879 2526
Size: 146 1733 647
Fragments arranged by size:
1733 647 146
SexAI A'CCwGG_T
Cuts at: 0 1157 1505 2526
Size: 1157 348 1021
Fragments arranged by size:
1157 1021 348
SfcI C'TryA_G
Cuts at: 0 147 223 781 855 1573 1650 1816 2105
Size: 147 76 558 74 718 77 166 289
Cuts at: 2105 2470 2526
Size: 365 56
Fragments arranged by size:
718 558 365 289 166 147 77 76
74 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 275 2526
Size: 275 2251
SfoI GGC'GCC
Cuts at: 0 354 1090 2526
Size: 354 736 1436
Fragments arranged by size:
1436 736 354
SmaI CCC'GGG
Cuts at: 0 295 1348 2526
Size: 295 1053 1178
Fragments arranged by size:
1178 1053 295
SmlI C'TyrA_G
Cuts at: 0 764 934 2224 2526
Size: 764 170 1290 302
Fragments arranged by size:
1290 764 302 170
StuI AGG'CCT
Cuts at: 0 1978 2526
Size: 1978 548
StyI C'CwwG_G
Cuts at: 0 128 892 2516 2526
Size: 128 764 1624 10
Fragments arranged by size:
1624 764 128 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 680 2526
Size: 555 125 1846
Fragments arranged by size:
1846 555 125
TatI w'GTAC_w
Cuts at: 0 964 1619 2526
Size: 964 655 907
Fragments arranged by size:
964 907 655
Tth111I GACn'n_nGTC
Cuts at: 0 626 2017 2526
Size: 626 1391 509
Fragments arranged by size:
1391 626 509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 518 2337 2526
Size: 518 1819 189
Fragments arranged by size:
1819 518 189
XhoI C'TCGA_G
Cuts at: 0 764 2526
Size: 764 1762
XmaI C'CCGG_G
Cuts at: 0 293 1346 2526
Size: 293 1053 1180
Fragments arranged by size:
1180 1053 293
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI
HaeII Hin4I KasI MfeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI
PpuMI PspOMI PspXI PstI PvuII SacI SapI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI
BglII BmtI BsaBI BsiWI BsmI BspEI BssHII BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV
FalI FspAI HincII HindIII HpaI KpnI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XmnI ZraI