Restriction map for
pF1KE2492
(Linear) (Six-base) MAPSORT of: KIEE2492.seq Check: 3513 from: 1 to: 5364
KIEE2492.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1296 1347 5322 5364
Size: 1296 51 3975 42
Fragments arranged by size:
3975 1296 51 42
AatII G_ACGT'C
Cuts at: 0 2749 5165 5364
Size: 2749 2416 199
Fragments arranged by size:
2749 2416 199
AccI GT'mk_AC
Cuts at: 0 1360 4157 5364
Size: 1360 2797 1207
Fragments arranged by size:
2797 1360 1207
Acc65I G'GTAC_C
Cuts at: 0 271 5364
Size: 271 5093
AclI AA'CG_TT
Cuts at: 0 2873 5364
Size: 2873 2491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1061 2536 2830 2958 3796 5263 5362
Size: 740 321 1475 294 128 838 1467 99
Cuts at: 5362 5364
Size: 2
Fragments arranged by size:
1475 1467 838 740 321 294 128 99
2
AflIII A'CryG_T
Cuts at: 0 3078 5364
Size: 3078 2286
AleI CACnn'nnGTG
Cuts at: 0 721 982 1354 4096 4616 5152 5364
Size: 721 261 372 2742 520 536 212
Fragments arranged by size:
2742 721 536 520 372 261 212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1091 5364
Size: 1091 4273
AlwNI CAG_nnn'CTG
Cuts at: 0 530 1930 5364
Size: 530 1400 3434
Fragments arranged by size:
3434 1400 530
ApaI G_GGCC'C
Cuts at: 0 4248 5364
Size: 4248 1116
ApaLI G'TGCA_C
Cuts at: 0 828 4315 5364
Size: 828 3487 1049
Fragments arranged by size:
3487 1049 828
ApoI r'AATT_y
Cuts at: 0 1470 1771 2650 3467 4003 4404 5364
Size: 1470 301 879 817 536 401 960
Fragments arranged by size:
1470 960 879 817 536 401 301
AseI AT'TA_AT
Cuts at: 0 3290 5364
Size: 3290 2074
AvaI C'yCGr_G
Cuts at: 0 376 502 1753 3104 3799 4320 4787 5364
Size: 376 126 1251 1351 695 521 467 577
Fragments arranged by size:
1351 1251 695 577 521 467 376 126
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3493 3526 5364
Size: 3493 33 1838
Fragments arranged by size:
3493 1838 33
BamHI G'GATC_C
Cuts at: 0 372 3645 5364
Size: 372 3273 1719
Fragments arranged by size:
3273 1719 372
BanI G'GyrC_C
Cuts at: 0 26 271 2184 2446 3892 4389 5364
Size: 26 245 1913 262 1446 497 975
Fragments arranged by size:
1913 1446 975 497 262 245 26
BanII G_rGCy'C
Cuts at: 0 524 784 1904 4248 4277 4794 5057 5364
Size: 524 260 1120 2344 29 517 263 307
Fragments arranged by size:
2344 1120 524 517 307 263 260 29
BbeI G_GCGC'C
Cuts at: 0 30 2188 5364
Size: 30 2158 3176
Fragments arranged by size:
3176 2158 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 2323 2741 2909 3081 3830 5165 5276
Size: 116 2207 418 168 172 749 1335 111
Cuts at: 5276 5364
Size: 88
Fragments arranged by size:
2207 1335 749 418 172 168 116 111
88
BbvCI CC'TCA_GC
Cuts at: 0 4738 5364
Size: 4738 626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 329 809 843 2713 2747 5364
Size: 295 34 480 34 1870 34 2617
Fragments arranged by size:
2617 1870 480 295 34 34 34
BglII A'GATC_T
Cuts at: 0 1398 2838 4830 5252 5364
Size: 1398 1440 1992 422 112
Fragments arranged by size:
1992 1440 1398 422 112
BlpI GC'TnA_GC
Cuts at: 0 892 1130 5364
Size: 892 238 4234
Fragments arranged by size:
4234 892 238
Bme1580I G_kGCm'C
Cuts at: 0 832 3038 4204 4248 4319 4394 5364
Size: 832 2206 1166 44 71 75 970
Fragments arranged by size:
2206 1166 970 832 75 71 44
BmgBI CAC'GTC
Cuts at: 0 4205 5364
Size: 4205 1159
BmrI ACTGGGnnnn_n'
Cuts at: 0 1300 2346 2407 3468 3708 5364
Size: 1300 1046 61 1061 240 1656
Fragments arranged by size:
1656 1300 1061 1046 240 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 402 696 2857 5364
Size: 402 294 2161 2507
Fragments arranged by size:
2507 2161 402 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 313 1466 2060 3380 3671 4246 4969
Size: 124 189 1153 594 1320 291 575 723
Cuts at: 4969 5071 5364
Size: 102 293
Fragments arranged by size:
1320 1153 723 594 575 293 291 189
124 102
Bpu10I CC'TnA_GC
Cuts at: 0 523 1092 4738 5364
Size: 523 569 3646 626
Fragments arranged by size:
3646 626 569 523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1833 4993 5269 5364
Size: 954 879 3160 276 95
Fragments arranged by size:
3160 954 879 276 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 3356 5364
Size: 3356 2008
BsaAI yAC'GTr
Cuts at: 0 3534 5364
Size: 3534 1830
BsaHI Gr'CG_yC
Cuts at: 0 27 2185 2392 2746 5162 5364
Size: 27 2158 207 354 2416 202
Fragments arranged by size:
2416 2158 354 207 202 27
BsaWI w'CCGG_w
Cuts at: 0 238 4958 5364
Size: 238 4720 406
Fragments arranged by size:
4720 406 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 1365 1395 3899 3929 5364
Size: 739 30 596 30 2504 30 1435
Fragments arranged by size:
2504 1435 739 596 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 699 744 1266 2290 2320 2345 4737
Size: 190 509 45 522 1024 30 25 2392
Cuts at: 4737 5364
Size: 627
Fragments arranged by size:
2392 1024 627 522 509 190 45 30
25
BseYI C'CCAG_C
Cuts at: 0 663 1025 1199 1930 2083 3812 4847 4946
Size: 663 362 174 731 153 1729 1035 99
Cuts at: 4946 5080 5084 5364
Size: 134 4 280
Fragments arranged by size:
1729 1035 731 663 362 280 174 153
134 99 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 210 1330 2185 3103 5364
Size: 60 150 1120 855 918 2261
Fragments arranged by size:
2261 1120 918 855 150 60
BsiEI CG_ry'CG
Cuts at: 0 162 3921 4959 5364
Size: 162 3759 1038 405
Fragments arranged by size:
3759 1038 405 162
BsiHKAI G_wGCw'C
Cuts at: 0 832 2588 2765 3481 4093 4124 4319 4985
Size: 832 1756 177 716 612 31 195 666
Cuts at: 4985 5093 5364
Size: 108 271
Fragments arranged by size:
1756 832 716 666 612 271 195 177
108 31
BsmI GAATG_Cn'
Cuts at: 0 1493 1797 3661 5364
Size: 1493 304 1864 1703
Fragments arranged by size:
1864 1703 1493 304
BsmBI CGTCTCn'nnnn_
Cuts at: 0 505 3427 3727 5364
Size: 505 2922 300 1637
Fragments arranged by size:
2922 1637 505 300
Bsp1286I G_dGCh'C
Cuts at: 0 524 784 832 1904 2588 2765 3038 3481
Size: 524 260 48 1072 684 177 273 443
Cuts at: 3481 4093 4124 4204 4248 4277 4319 4394 4794
Size: 612 31 80 44 29 42 75 400
Cuts at: 4794 4985 5057 5093 5364
Size: 191 72 36 271
Fragments arranged by size:
1072 684 612 524 443 400 273 271
260 191 177 80 75 72 48 44
42 36 31 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1296 1347 5322 5364
Size: 1296 51 3975 42
Fragments arranged by size:
3975 1296 51 42
BsrBI CCG'CTC
Cuts at: 0 1885 2335 5364
Size: 1885 450 3029
Fragments arranged by size:
3029 1885 450
BsrDI GCAATG_nn'
Cuts at: 0 1063 5364
Size: 1063 4301
BsrFI r'CCGG_y
Cuts at: 0 535 2187 2596 4138 4175 5364
Size: 535 1652 409 1542 37 1189
Fragments arranged by size:
1652 1542 1189 535 409 37
BsrGI T'GTAC_A
Cuts at: 0 4835 5364
Size: 4835 529
BssHII G'CGCG_C
Cuts at: 0 4841 5364
Size: 4841 523
BssSI C'ACGA_G
Cuts at: 0 1242 4612 4879 5152 5364
Size: 1242 3370 267 273 212
Fragments arranged by size:
3370 1242 273 267 212
BstEII G'GTnAC_C
Cuts at: 0 793 5364
Size: 793 4571
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 2469 3096 3358 5364
Size: 30 2439 627 262 2006
Fragments arranged by size:
2439 2006 627 262 30
BstYI r'GATC_y
Cuts at: 0 372 1398 2838 3645 4698 4830 5252 5364
Size: 372 1026 1440 807 1053 132 422 112
Fragments arranged by size:
1440 1053 1026 807 422 372 132 112
Bsu36I CC'TnA_GG
Cuts at: 0 812 4438 4656 5364
Size: 812 3626 218 708
Fragments arranged by size:
3626 812 708 218
BtgI C'CryG_G
Cuts at: 0 246 275 727 1373 1778 2753 3921 3980
Size: 246 29 452 646 405 975 1168 59
Cuts at: 3980 4394 4454 5364
Size: 414 60 910
Fragments arranged by size:
1168 975 910 646 452 414 405 246
60 59 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1038 2041 4984 5088 5364
Size: 1038 1003 2943 104 276
Fragments arranged by size:
2943 1038 1003 276 104
BtsI GCAGTG_nn'
Cuts at: 0 3153 4103 4297 5364
Size: 3153 950 194 1067
Fragments arranged by size:
3153 1067 950 194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4342 4377 4755 4790 4885 4920 5364
Size: 4342 35 378 35 95 35 444
Fragments arranged by size:
4342 444 378 95 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 4128 4884 5364
Size: 4128 756 480
Fragments arranged by size:
4128 756 480
DrdI GACnn_nn'nnGTC
Cuts at: 0 2713 5364
Size: 2713 2651
EaeI y'GGCC_r
Cuts at: 0 159 1166 2189 2336 2756 3336 3918 5198
Size: 159 1007 1023 147 420 580 582 1280
Cuts at: 5198 5210 5364
Size: 12 154
Fragments arranged by size:
1280 1023 1007 582 580 420 159 154
147 12
EagI C'GGCC_G
Cuts at: 0 159 3918 5364
Size: 159 3759 1446
Fragments arranged by size:
3759 1446 159
EarI CTCTTCn'nnn_
Cuts at: 0 167 464 582 1037 1707 1829 3772 4328
Size: 167 297 118 455 670 122 1943 556
Cuts at: 4328 4957 5364
Size: 629 407
Fragments arranged by size:
1943 670 629 556 455 407 297 167
122 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4923 5364
Size: 4923 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 313 740 1061 1466 2060 2536 2830
Size: 124 189 427 321 405 594 476 294
Cuts at: 2830 2958 3380 3671 3796 4246 4969 5071 5263
Size: 128 422 291 125 450 723 102 192
Cuts at: 5263 5362 5364
Size: 99 2
Fragments arranged by size:
723 594 476 450 427 422 405 321
294 291 192 189 128 125 124 102
99 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 763 3042 4659 5364
Size: 103 660 2279 1617 705
Fragments arranged by size:
2279 1617 705 660 103
EcoO109I rG'GnC_Cy
Cuts at: 0 527 1016 1622 2067 4244 4479 5364
Size: 527 489 606 445 2177 235 885
Fragments arranged by size:
2177 885 606 527 489 445 235
EcoRI G'AATT_C
Cuts at: 0 4003 5364
Size: 4003 1361
EcoRV GAT'ATC
Cuts at: 0 2478 5364
Size: 2478 2886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1197 2860 5364
Size: 1197 1663 2504
Fragments arranged by size:
2504 1663 1197
HaeII r_GCGC'y
Cuts at: 0 30 2188 2790 3032 3118 4213 5364
Size: 30 2158 602 242 86 1095 1151
Fragments arranged by size:
2158 1151 1095 602 242 86 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 382 389 414 3899 3931 4612 4644
Size: 357 25 7 25 3485 32 681 32
Cuts at: 4644 5087 5119 5364
Size: 443 32 245
Fragments arranged by size:
3485 681 443 357 245 32 32 32
25 25 7
HincII GTy'rAC
Cuts at: 0 3000 4473 4869 5364
Size: 3000 1473 396 495
Fragments arranged by size:
3000 1473 495 396
HindIII A'AGCT_T
Cuts at: 0 1161 1508 3325 5245 5364
Size: 1161 347 1817 1920 119
Fragments arranged by size:
1920 1817 1161 347 119
KasI G'GCGC_C
Cuts at: 0 26 2184 5364
Size: 26 2158 3180
Fragments arranged by size:
3180 2158 26
KpnI G_GTAC'C
Cuts at: 0 275 5364
Size: 275 5089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 2501 2945 2951 4512 4979 5364
Size: 605 1896 444 6 1561 467 385
Fragments arranged by size:
1896 1561 605 467 444 385 6
MscI TGG'CCA
Cuts at: 0 3338 5212 5364
Size: 3338 1874 152
Fragments arranged by size:
3338 1874 152
MslI CAynn'nnrTG
Cuts at: 0 566 721 982 1354 2296 3302 4096 4459
Size: 566 155 261 372 942 1006 794 363
Cuts at: 4459 4616 5071 5119 5152 5364
Size: 157 455 48 33 212
Fragments arranged by size:
1006 942 794 566 455 372 363 261
212 157 155 48 33
MspA1I CmG'CkG
Cuts at: 0 345 982 1927 3585 4543 4946 5084 5196
Size: 345 637 945 1658 958 403 138 112
Cuts at: 5196 5364
Size: 168
Fragments arranged by size:
1658 958 945 637 403 345 168 138
112
NaeI GCC'GGC
Cuts at: 0 2189 4140 4177 5364
Size: 2189 1951 37 1187
Fragments arranged by size:
2189 1951 1187 37
NarI GG'CG_CC
Cuts at: 0 27 2185 5364
Size: 27 2158 3179
Fragments arranged by size:
3179 2158 27
NcoI C'CATG_G
Cuts at: 0 275 1778 4454 5364
Size: 275 1503 2676 910
Fragments arranged by size:
2676 1503 910 275
NgoMIV G'CCGG_C
Cuts at: 0 2187 4138 4175 5364
Size: 2187 1951 37 1189
Fragments arranged by size:
2187 1951 1189 37
NspI r_CATG'y
Cuts at: 0 3082 4692 5364
Size: 3082 1610 672
Fragments arranged by size:
3082 1610 672
PciI A'CATG_T
Cuts at: 0 3078 5364
Size: 3078 2286
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 5364
Size: 291 5073
PfoI T'CCnGG_A
Cuts at: 0 415 775 1966 3648 3899 3998 4701 5222
Size: 415 360 1191 1682 251 99 703 521
Cuts at: 5222 5364
Size: 142
Fragments arranged by size:
1682 1191 703 521 415 360 251 142
99
PpuMI rG'GwC_Cy
Cuts at: 0 527 1016 1622 5364
Size: 527 489 606 3742
Fragments arranged by size:
3742 606 527 489
PshAI GACnn'nnGTC
Cuts at: 0 3838 5364
Size: 3838 1526
PsiI TTA'TAA
Cuts at: 0 2672 5364
Size: 2672 2692
PspOMI G'GGCC_C
Cuts at: 0 4244 5364
Size: 4244 1120
PspXI vC'TCGA_Gb
Cuts at: 0 3104 5364
Size: 3104 2260
PstI C_TGCA'G
Cuts at: 0 764 1010 4292 4826 5364
Size: 764 246 3282 534 538
Fragments arranged by size:
3282 764 538 534 246
PvuII CAG'CTG
Cuts at: 0 345 982 1927 3585 5084 5364
Size: 345 637 945 1658 1499 280
Fragments arranged by size:
1658 1499 945 637 345 280
SapI GCTCTTCn'nnn_
Cuts at: 0 582 5364
Size: 582 4782
SbfI CC_TGCA'GG
Cuts at: 0 4826 5364
Size: 4826 538
ScaI AGT'ACT
Cuts at: 0 4990 5364
Size: 4990 374
SfcI C'TryA_G
Cuts at: 0 760 1006 4011 4288 4428 4822 5364
Size: 760 246 3005 277 140 394 542
Fragments arranged by size:
3005 760 542 394 277 246 140
SfoI GGC'GCC
Cuts at: 0 28 2186 5364
Size: 28 2158 3178
Fragments arranged by size:
3178 2158 28
SmaI CCC'GGG
Cuts at: 0 504 3801 5364
Size: 504 3297 1563
Fragments arranged by size:
3297 1563 504
SmlI C'TyrA_G
Cuts at: 0 969 1812 3104 5008 5248 5364
Size: 969 843 1292 1904 240 116
Fragments arranged by size:
1904 1292 969 843 240 116
SnaBI TAC'GTA
Cuts at: 0 3534 5364
Size: 3534 1830
SphI G_CATG'C
Cuts at: 0 4692 5364
Size: 4692 672
StuI AGG'CCT
Cuts at: 0 1072 1090 2311 2800 3208 5357 5364
Size: 1072 18 1221 489 408 2149 7
Fragments arranged by size:
2149 1221 1072 489 408 18 7
StyI C'CwwG_G
Cuts at: 0 275 545 1097 1778 4454 5364
Size: 275 270 552 681 2676 910
Fragments arranged by size:
2676 910 681 552 275 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 523 556 1039 1762 2097 2221 2439
Size: 298 225 33 483 723 335 124 218
Cuts at: 2439 2751 3322 5364
Size: 312 571 2042
Fragments arranged by size:
2042 723 571 483 335 312 298 225
218 124 33
TatI w'GTAC_w
Cuts at: 0 4370 4835 4988 5364
Size: 4370 465 153 376
Fragments arranged by size:
4370 465 376 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 1365 1397 5364
Size: 739 32 594 32 3967
Fragments arranged by size:
3967 739 594 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 620 5364
Size: 620 4744
XbaI T'CTAG_A
Cuts at: 0 198 5364
Size: 198 5166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1044 1161 3501 3978 4452 4643 5193 5364
Size: 1044 117 2340 477 474 191 550 171
Fragments arranged by size:
2340 1044 550 477 474 191 171 117
XhoI C'TCGA_G
Cuts at: 0 3104 5364
Size: 3104 2260
XmaI C'CCGG_G
Cuts at: 0 502 3799 5364
Size: 502 3297 1565
Fragments arranged by size:
3297 1565 502
XmnI GAAnn'nnTTC
Cuts at: 0 2537 5364
Size: 2537 2827
ZraI GAC'GTC
Cuts at: 0 2747 5163 5364
Size: 2747 2416 201
Fragments arranged by size:
2747 2416 201
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuII SapI SbfI ScaI SfcI SfoI SmaI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI AscI AsiSI AvrII
BciVI BclI BfrBI BglI BmtI BsaBI BsiWI BspEI
BspHI BstAPI BstBI BstZ17I ClaI DraI EcoICRI FseI
FspI FspAI HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PsrI
PvuI RsrII SacI SacII SalI SanDI SexAI SfiI
SgfI SgrAI SpeI SrfI SspI SwaI