Restriction map for
pF1KE2499
(Linear) (Six-base) MAPSORT of: KIEE2499.seq Check: 3957 from: 1 to: 13722
KIEE2499.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2520 4575 5835 6399 6618 6894 7926 10737
Size: 2520 2055 1260 564 219 276 1032 2811
Cuts at: 10737 13396 13722
Size: 2659 326
Fragments arranged by size:
2811 2659 2520 2055 1260 1032 564 326
276 219
AatII G_ACGT'C
Cuts at: 0 8792 12472 13722
Size: 8792 3680 1250
Fragments arranged by size:
8792 3680 1250
AccI GT'mk_AC
Cuts at: 0 8765 11165 13722
Size: 8765 2400 2557
Fragments arranged by size:
8765 2557 2400
Acc65I G'GTAC_C
Cuts at: 0 602 13722
Size: 602 13120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 940 1066 1246 1576 2101 2254 2482
Size: 56 884 126 180 330 525 153 228
Cuts at: 2482 2851 3496 5890 6451 6736 8209 9232 9460
Size: 369 645 2394 561 285 1473 1023 228
Cuts at: 9460 9978 10545 12049 12205 12463 13222 13722
Size: 518 567 1504 156 258 759 500
Fragments arranged by size:
2394 1504 1473 1023 884 759 645 567
561 525 518 500 369 330 285 258
228 228 180 156 153 126 56
AfeI AGC'GCT
Cuts at: 0 3031 3607 3640 4573 4861 4978 6871 7218
Size: 3031 576 33 933 288 117 1893 347
Cuts at: 7218 7294 7600 8689 9094 13066 13237 13722
Size: 76 306 1089 405 3972 171 485
Fragments arranged by size:
3972 3031 1893 1089 933 576 485 405
347 306 288 171 117 76 33
AflIII A'CryG_T
Cuts at: 0 4878 13722
Size: 4878 8844
AgeI A'CCGG_T
Cuts at: 0 11998 13057 13722
Size: 11998 1059 665
Fragments arranged by size:
11998 1059 665
AhdI GACnn_n'nnGTC
Cuts at: 0 825 11589 13722
Size: 825 10764 2133
Fragments arranged by size:
10764 2133 825
AleI CACnn'nnGTG
Cuts at: 0 269 2552 3132 8113 8965 10747 13722
Size: 269 2283 580 4981 852 1782 2975
Fragments arranged by size:
4981 2975 2283 1782 852 580 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4438 4814 7958 8509 9037 10486 12220 13477
Size: 4438 376 3144 551 528 1449 1734 1257
Cuts at: 13477 13722
Size: 245
Fragments arranged by size:
4438 3144 1734 1449 1257 551 528 376
245
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 983 1014 1015 13722
Size: 982 1 31 1 12707
Fragments arranged by size:
12707 982 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 34 612 1044 1662 2301 2715 2982 2985
Size: 34 578 432 618 639 414 267 3
Cuts at: 2985 2994 3840 4353 4512 4692 5205 5274 5337
Size: 9 846 513 159 180 513 69 63
Cuts at: 5337 5445 6285 6612 6642 6714 7149 7380 7533
Size: 108 840 327 30 72 435 231 153
Cuts at: 7533 7551 7668 7722 7806 7860 7881 8052 8241
Size: 18 117 54 84 54 21 171 189
Cuts at: 8241 8682 9078 9192 9438 9471 9888 10145 10299
Size: 441 396 114 246 33 417 257 154
Cuts at: 10299 10359 10659 10989 11190 13011 13200 13257 13353
Size: 60 300 330 201 1821 189 57 96
Cuts at: 13353 13722
Size: 369
Fragments arranged by size:
1821 846 840 639 618 578 513 513
441 435 432 417 414 396 369 330
327 300 267 257 246 231 201 189
189 180 171 159 154 153 117 114
108 96 84 72 69 63 60 57
54 54 34 33 30 21 18 9
3
ApaI G_GGCC'C
Cuts at: 0 2578 3402 3492 4683 8362 9346 9591 9738
Size: 2578 824 90 1191 3679 984 245 147
Cuts at: 9738 10339 10887 11345 12073 12916 13705 13722
Size: 601 548 458 728 843 789 17
Fragments arranged by size:
3679 2578 1191 984 843 824 789 728
601 548 458 245 147 90 17
ApaLI G'TGCA_C
Cuts at: 0 2545 3005 3091 4517 8536 10513 13722
Size: 2545 460 86 1426 4019 1977 3209
Fragments arranged by size:
4019 3209 2545 1977 1426 460 86
ApoI r'AATT_y
Cuts at: 0 1780 6595 13722
Size: 1780 4815 7127
Fragments arranged by size:
7127 4815 1780
AvaI C'yCGr_G
Cuts at: 0 330 347 1624 1838 2923 3454 3685 6368
Size: 330 17 1277 214 1085 531 231 2683
Cuts at: 6368 6461 8362 8704 8783 9346 9458 9557 9731
Size: 93 1901 342 79 563 112 99 174
Cuts at: 9731 10339 10401 10681 10870 11059 11110 11344 12073
Size: 608 62 280 189 189 51 234 729
Cuts at: 12073 12517 12789 13571 13653 13677 13722
Size: 444 272 782 82 24 45
Fragments arranged by size:
2683 1901 1277 1085 782 729 608 563
531 444 342 330 280 272 234 231
214 189 189 174 112 99 93 82
79 62 51 45 24 17
BamHI G'GATC_C
Cuts at: 0 12832 13722
Size: 12832 890
BanI G'GyrC_C
Cuts at: 0 413 602 1149 1469 1619 1843 2652 2729
Size: 413 189 547 320 150 224 809 77
Cuts at: 2729 3739 4730 5188 6026 6146 9021 9145 9268
Size: 1010 991 458 838 120 2875 124 123
Cuts at: 9268 9597 9674 11086 11669 11686 11872 12680 13366
Size: 329 77 1412 583 17 186 808 686
Cuts at: 13366 13722
Size: 356
Fragments arranged by size:
2875 1412 1010 991 838 809 808 686
583 547 458 413 356 329 320 224
189 186 150 124 123 120 77 77
17
BanII G_rGCy'C
Cuts at: 0 2105 2578 3047 3402 3455 3492 3686 4366
Size: 2105 473 469 355 53 37 194 680
Cuts at: 4366 4683 6468 6770 7266 7346 8126 8362 9068
Size: 317 1785 302 496 80 780 236 706
Cuts at: 9068 9138 9346 9591 9665 9738 9752 9848 10031
Size: 70 208 245 74 73 14 96 183
Cuts at: 10031 10059 10117 10339 10887 11152 11345 11600 11978
Size: 28 58 222 548 265 193 255 378
Cuts at: 11978 12073 12916 13280 13705 13722
Size: 95 843 364 425 17
Fragments arranged by size:
2105 1785 843 780 706 680 548 496
473 469 425 378 364 355 317 302
265 255 245 236 222 208 194 193
183 96 95 80 74 73 70 58
53 37 28 17 14
BbeI G_GCGC'C
Cuts at: 0 1623 3743 4734 6030 6150 12684 13370 13722
Size: 1623 2120 991 1296 120 6534 686 352
Fragments arranged by size:
6534 2120 1623 1296 991 686 352 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 1252 4849 5552 8144 10969 11886 12472
Size: 290 962 3597 703 2592 2825 917 586
Cuts at: 12472 13722
Size: 1250
Fragments arranged by size:
3597 2825 2592 1250 962 917 703 586
290
BbvCI CC'TCA_GC
Cuts at: 0 17 812 5402 5830 6038 6350 7061 7220
Size: 17 795 4590 428 208 312 711 159
Cuts at: 7220 7277 7844 10048 10727 11027 11644 13722
Size: 57 567 2204 679 300 617 2078
Fragments arranged by size:
4590 2204 2078 795 711 679 617 567
428 312 300 208 159 57 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2758 2792 3180 3214 4239 4273 7326 7360
Size: 2758 34 388 34 1025 34 3053 34
Cuts at: 7360 8154 8188 9036 9070 13722
Size: 794 34 848 34 4652
Fragments arranged by size:
4652 3053 2758 1025 848 794 388 34
34 34 34 34 34
BclI T'GATC_A
Cuts at: 0 10934 12659 13722
Size: 10934 1725 1063
Fragments arranged by size:
10934 1725 1063
BglI GCCn_nnn'nGGC
Cuts at: 0 1078 1757 2440 2680 3436 3466 3976 4396
Size: 1078 679 683 240 756 30 510 420
Cuts at: 4396 4484 4660 4687 4945 4999 5350 5392 5485
Size: 88 176 27 258 54 351 42 93
Cuts at: 5485 6202 6343 6733 6946 7906 9341 9379 9595
Size: 717 141 390 213 960 1435 38 216
Cuts at: 9595 10154 10231 10321 10372 10823 11236 11491 13141
Size: 559 77 90 51 451 413 255 1650
Cuts at: 13141 13195 13722
Size: 54 527
Fragments arranged by size:
1650 1435 1078 960 756 717 683 679
559 527 510 451 420 413 390 351
258 255 240 216 213 176 141 93
90 88 77 54 54 51 42 38
30 27
BglII A'GATC_T
Cuts at: 0 515 8435 13409 13722
Size: 515 7920 4974 313
Fragments arranged by size:
7920 4974 515 313
BlpI GC'TnA_GC
Cuts at: 0 167 2605 2942 4133 4409 4910 6799 7226
Size: 167 2438 337 1191 276 501 1889 427
Cuts at: 7226 7439 9700 11372 13722
Size: 213 2261 1672 2350
Fragments arranged by size:
2438 2350 2261 1889 1672 1191 501 427
337 276 213 167
Bme1580I G_kGCm'C
Cuts at: 0 1154 1806 2549 2578 2636 2657 3009 3095
Size: 1154 652 743 29 58 21 352 86
Cuts at: 3095 3141 3402 3492 3549 4521 4683 4923 5505
Size: 46 261 90 57 972 162 240 582
Cuts at: 5505 6531 8362 8540 8555 9278 9346 9539 9591
Size: 1026 1831 178 15 723 68 193 52
Cuts at: 9591 9738 10011 10339 10517 10887 11089 11345 12073
Size: 147 273 328 178 370 202 256 728
Cuts at: 12073 12916 13301 13456 13705 13722
Size: 843 385 155 249 17
Fragments arranged by size:
1831 1154 1026 972 843 743 728 723
652 582 385 370 352 328 273 261
256 249 240 202 193 178 178 162
155 147 90 86 68 58 57 52
46 29 21 17 15
BmgBI CAC'GTC
Cuts at: 0 801 882 1446 2787 3594 3669 8142 8562
Size: 801 81 564 1341 807 75 4473 420
Cuts at: 8562 10539 13362 13722
Size: 1977 2823 360
Fragments arranged by size:
4473 2823 1977 1341 807 801 564 420
360 81 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 3703 10053 12125 13722
Size: 3703 6350 2072 1597
Fragments arranged by size:
6350 3703 2072 1597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 2070 2973 4650 5433 6789 9771 10131
Size: 345 1725 903 1677 783 1356 2982 360
Cuts at: 10131 13722
Size: 3591
Fragments arranged by size:
3591 2982 1725 1677 1356 903 783 360
345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 618 660 748 990 1138 1234 1273
Size: 340 278 42 88 242 148 96 39
Cuts at: 1273 1651 1945 2031 2095 2380 2716 2887 3088
Size: 378 294 86 64 285 336 171 201
Cuts at: 3088 3301 3322 3355 3865 3916 4207 4714 4801
Size: 213 21 33 510 51 291 507 87
Cuts at: 4801 4876 4993 5182 5296 5467 5740 5818 5932
Size: 75 117 189 114 171 273 78 114
Cuts at: 5932 5938 6001 6124 6685 7204 7228 7402 7465
Size: 6 63 123 561 519 24 174 63
Cuts at: 7465 7648 7828 7903 8290 8518 8704 9061 9193
Size: 183 180 75 387 228 186 357 132
Cuts at: 9193 9649 10186 10267 10495 10681 11272 11560 12001
Size: 456 537 81 228 186 591 288 441
Cuts at: 12001 12229 12607 12852 12967 13036 13264 13492 13722
Size: 228 378 245 115 69 228 228 230
Fragments arranged by size:
591 561 537 519 510 507 456 441
387 378 378 357 340 336 294 291
288 285 278 273 245 242 230 228
228 228 228 228 213 201 189 186
186 183 180 174 171 171 148 132
123 117 115 114 114 96 88 87
86 81 78 75 75 69 64 63
63 51 42 39 33 24 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 17 812 3394 5402 5830 6038 6350 6467
Size: 17 795 2582 2008 428 208 312 117
Cuts at: 6467 7061 7220 7277 7844 10048 10727 11027 11644
Size: 594 159 57 567 2204 679 300 617
Cuts at: 11644 11662 13722
Size: 18 2060
Fragments arranged by size:
2582 2204 2060 2008 795 679 617 594
567 428 312 300 208 159 117 57
18 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1441 3328 3400 3655 3979 4972 5608
Size: 1129 312 1887 72 255 324 993 636
Cuts at: 5608 5632 6793 7000 7039 7219 7477 10057 11194
Size: 24 1161 207 39 180 258 2580 1137
Cuts at: 11194 13390 13722
Size: 2196 332
Fragments arranged by size:
2580 2196 1887 1161 1137 1129 993 636
332 324 312 258 255 207 180 72
39 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1040 6664 9571 9769 9961 10385 10732 13722
Size: 1040 5624 2907 198 192 424 347 2990
Fragments arranged by size:
5624 2990 2907 1040 424 347 198 192
BsaAI yAC'GTr
Cuts at: 0 1203 2742 4029 4881 8669 10646 11007 11619
Size: 1203 1539 1287 852 3788 1977 361 612
Cuts at: 11619 13722
Size: 2103
Fragments arranged by size:
3788 2103 1977 1539 1287 1203 852 612
361
BsaBI GATnn'nnATC
Cuts at: 0 1024 8956 10939 12625 13722
Size: 1024 7932 1983 1686 1097
Fragments arranged by size:
7932 1983 1686 1097 1024
BsaHI Gr'CG_yC
Cuts at: 0 860 1328 1620 3521 3740 3755 4731 6027
Size: 860 468 292 1901 219 15 976 1296
Cuts at: 6027 6147 7286 8789 9485 9611 11717 11903 12469
Size: 120 1139 1503 696 126 2106 186 566
Cuts at: 12469 12681 13082 13334 13367 13601 13722
Size: 212 401 252 33 234 121
Fragments arranged by size:
2106 1901 1503 1296 1139 976 860 696
566 468 401 292 252 234 219 212
186 126 121 120 33 15
BsaWI w'CCGG_w
Cuts at: 0 7298 11998 12977 13057 13722
Size: 7298 4700 979 80 665
Fragments arranged by size:
7298 4700 979 665 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 351 381 616 646 1171 1201
Size: 295 30 26 30 235 30 525 30
Cuts at: 1201 1954 1984 5206 5236 6157 6187 8758 8788
Size: 753 30 3222 30 921 30 2571 30
Cuts at: 8788 9973 10003 10171 10201 10810 10840 11085 11115
Size: 1185 30 168 30 609 30 245 30
Cuts at: 11115 12595 12625 12658 12688 12940 12970 13237 13267
Size: 1480 30 33 30 252 30 267 30
Cuts at: 13267 13722
Size: 455
Fragments arranged by size:
3222 2571 1480 1185 921 753 609 525
455 295 267 252 245 235 168 33
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1030 1321 1693 1890 2002 2005 2008 2854
Size: 1030 291 372 197 112 3 3 846
Cuts at: 2854 3400 3421 3862 3892 4129 4132 4135 4165
Size: 546 21 441 30 237 3 3 30
Cuts at: 4165 4291 4309 4354 4447 4450 4543 4576 4711
Size: 126 18 45 93 3 93 33 135
Cuts at: 4711 4732 4918 4969 5041 5095 5134 5293 5296
Size: 21 186 51 72 54 39 159 3
Cuts at: 5296 5419 5422 5497 5737 5770 5866 5869 6028
Size: 123 3 75 240 33 96 3 159
Cuts at: 6028 6031 6055 6085 6088 6151 6367 6376 6460
Size: 3 24 30 3 63 216 9 84
Cuts at: 6460 6682 6715 6775 6778 6808 6898 6928 6988
Size: 222 33 60 3 30 90 30 60
Cuts at: 6988 7171 7294 7327 7516 7690 7753 7783 7807
Size: 183 123 33 189 174 63 30 24
Cuts at: 7807 7846 7861 7900 7933 8095 8605 8986 9190
Size: 39 15 39 33 162 510 381 204
Cuts at: 9190 9589 9760 9973 10582 10774 10777 10888 11476
Size: 399 171 213 609 192 3 111 588
Cuts at: 11476 11779 11851 12058 12262 12316 12382 12621 12624
Size: 303 72 207 204 54 66 239 3
Cuts at: 12624 12631 12813 12828 12831 12919 13462 13638 13641
Size: 7 182 15 3 88 543 176 3
Cuts at: 13641 13722
Size: 81
Fragments arranged by size:
1030 846 609 588 546 543 510 441
399 381 372 303 291 240 239 237
222 216 213 207 204 204 197 192
189 186 183 182 176 174 171 162
159 159 135 126 123 123 112 111
96 93 93 90 88 84 81 75
72 72 66 63 63 60 60 54
54 51 45 39 39 39 33 33
33 33 33 30 30 30 30 30
30 24 24 21 21 18 15 15
9 7 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 67 136 162 1726 1743 2113 2475 2702
Size: 67 69 26 1564 17 370 362 227
Cuts at: 2702 3140 3186 3291 3626 5768 5922 7289 7661
Size: 438 46 105 335 2142 154 1367 372
Cuts at: 7661 8617 9488 9517 9631 10110 10594 10886 11486
Size: 956 871 29 114 479 484 292 600
Cuts at: 11486 11599 12875 13051 13722
Size: 113 1276 176 671
Fragments arranged by size:
2142 1564 1367 1276 956 871 671 600
484 479 438 372 370 362 335 292
227 176 154 114 113 105 69 67
46 29 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 490 904 1183 1468 1495 1633 1747
Size: 229 261 414 279 285 27 138 114
Cuts at: 1747 2476 2564 2809 2842 2857 3746 4609 4820
Size: 729 88 245 33 15 889 863 211
Cuts at: 4820 6070 6274 6394 6805 7057 7315 7393 7537
Size: 1250 204 120 411 252 258 78 144
Cuts at: 7537 8353 11335 13014 13270 13722
Size: 816 2982 1679 256 452
Fragments arranged by size:
2982 1679 1250 889 863 816 729 452
414 411 285 279 261 258 256 252
245 229 211 204 144 138 120 114
88 78 33 27 15
BsiEI CG_ry'CG
Cuts at: 0 651 980 2019 5463 5894 6068 8390 9060
Size: 651 329 1039 3444 431 174 2322 670
Cuts at: 9060 10914 11358 12101 12669 12726 13190 13665 13722
Size: 1854 444 743 568 57 464 475 57
Fragments arranged by size:
3444 2322 1854 1039 743 670 651 568
475 464 444 431 329 174 57 57
BsiHKAI G_wGCw'C
Cuts at: 0 2105 2549 2606 3009 3047 3095 3395 3455
Size: 2105 444 57 403 38 48 300 60
Cuts at: 3455 3686 4366 4521 6770 7346 8012 8126 8483
Size: 231 680 155 2249 576 666 114 357
Cuts at: 8483 8540 9068 9665 9752 9848 10031 10517 11978
Size: 57 528 597 87 96 183 486 1461
Cuts at: 11978 13722
Size: 1744
Fragments arranged by size:
2249 2105 1744 1461 680 666 597 576
528 486 444 403 357 300 231 183
155 114 96 87 60 57 57 48
38
BsiWI C'GTAC_G
Cuts at: 0 4039 13722
Size: 4039 9683
BsmI GAATG_Cn'
Cuts at: 0 4331 13722
Size: 4331 9391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 638 849 3073 4777 5236 7153 7534 8701
Size: 638 211 2224 1704 459 1917 381 1167
Cuts at: 8701 9098 9426 10678 12514 12870 12901 12934 13722
Size: 397 328 1252 1836 356 31 33 788
Fragments arranged by size:
2224 1917 1836 1704 1252 1167 788 638
459 397 381 356 328 211 33 31
Bsp1286I G_dGCh'C
Cuts at: 0 1154 1806 2105 2549 2578 2606 2636 2657
Size: 1154 652 299 444 29 28 30 21
Cuts at: 2657 3009 3047 3095 3141 3395 3402 3455 3492
Size: 352 38 48 46 254 7 53 37
Cuts at: 3492 3549 3686 4366 4521 4683 4923 5505 6468
Size: 57 137 680 155 162 240 582 963
Cuts at: 6468 6531 6770 7266 7346 8012 8126 8362 8483
Size: 63 239 496 80 666 114 236 121
Cuts at: 8483 8540 8555 9068 9138 9278 9346 9539 9591
Size: 57 15 513 70 140 68 193 52
Cuts at: 9591 9665 9738 9752 9848 10011 10031 10059 10117
Size: 74 73 14 96 163 20 28 58
Cuts at: 10117 10339 10517 10887 11089 11152 11345 11600 11978
Size: 222 178 370 202 63 193 255 378
Cuts at: 11978 12073 12916 13280 13301 13456 13705 13722
Size: 95 843 364 21 155 249 17
Fragments arranged by size:
1154 963 843 680 666 652 582 513
496 444 378 370 364 352 299 255
254 249 240 239 236 222 202 193
193 178 163 162 155 155 140 137
121 114 96 95 80 74 73 70
68 63 63 58 57 57 53 52
48 46 38 37 30 29 28 28
21 21 20 17 15 14 7
BspEI T'CCGG_A
Cuts at: 0 7298 13722
Size: 7298 6424
BspHI T'CATG_A
Cuts at: 0 9354 13722
Size: 9354 4368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 986 1661 1670 1730 1883 2375 2520 3297
Size: 986 675 9 60 153 492 145 777
Cuts at: 3297 4043 4575 4755 5835 6399 6618 6698 6894
Size: 746 532 180 1080 564 219 80 196
Cuts at: 6894 7926 8162 8681 8909 9230 10737 11081 11177
Size: 1032 236 519 228 321 1507 344 96
Cuts at: 11177 11772 12392 13396 13722
Size: 595 620 1004 326
Fragments arranged by size:
1507 1080 1032 1004 986 777 746 675
620 595 564 532 519 492 344 326
321 236 228 219 196 180 153 145
96 80 60 9
BsrBI CCG'CTC
Cuts at: 0 171 198 762 1126 1218 1800 2801 3588
Size: 171 27 564 364 92 582 1001 787
Cuts at: 3588 3618 4161 4242 4728 4914 4944 4965 5577
Size: 30 543 81 486 186 30 21 612
Cuts at: 5577 5802 6021 6117 6163 6426 7194 9714 10665
Size: 225 219 96 46 263 768 2520 951
Cuts at: 10665 11301 11376 12462 12674 12728 12812 13722
Size: 636 75 1086 212 54 84 910
Fragments arranged by size:
2520 1086 1001 951 910 787 768 636
612 582 564 543 486 364 263 225
219 212 186 171 96 92 84 81
75 54 46 30 30 27 21
BsrFI r'CCGG_y
Cuts at: 0 116 674 713 2120 2930 3123 3435 3963
Size: 116 558 39 1407 810 193 312 528
Cuts at: 3963 4418 5458 5462 8062 8315 8570 8990 9265
Size: 455 1040 4 2600 253 255 420 275
Cuts at: 9265 9645 9677 10153 10381 10909 11386 11497 11689
Size: 380 32 476 228 528 477 111 192
Cuts at: 11689 11998 12257 12751 12799 13057 13228 13261 13552
Size: 309 259 494 48 258 171 33 291
Cuts at: 13552 13564 13576 13606 13722
Size: 12 12 30 116
Fragments arranged by size:
2600 1407 1040 810 558 528 528 494
477 476 455 420 380 312 309 291
275 259 258 255 253 228 193 192
171 116 116 111 48 39 33 32
30 12 12 4
BsrGI T'GTAC_A
Cuts at: 0 10751 13722
Size: 10751 2971
BssHII G'CGCG_C
Cuts at: 0 4663 4948 8394 12105 13722
Size: 4663 285 3446 3711 1617
Fragments arranged by size:
4663 3711 3446 1617 285
BssSI C'ACGA_G
Cuts at: 0 1513 3403 8656 10309 10348 11335 13722
Size: 1513 1890 5253 1653 39 987 2387
Fragments arranged by size:
5253 2387 1890 1653 1513 987 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1611 1662 2301 2736 2985 3099 3651 3840
Size: 1611 51 639 435 249 114 552 189
Cuts at: 3840 4269 4353 4512 4516 6285 6303 6612 7035
Size: 429 84 159 4 1769 18 309 423
Cuts at: 7035 7515 7533 7551 9328 9471 10145 11879 13011
Size: 480 18 18 1777 143 674 1734 1132
Cuts at: 13011 13722
Size: 711
Fragments arranged by size:
1777 1769 1734 1611 1132 711 674 639
552 480 435 429 423 309 249 189
159 143 114 84 51 18 18 18
4
BstEII G'GTnAC_C
Cuts at: 0 7362 8844 9915 10917 11382 11841 13722
Size: 7362 1482 1071 1002 465 459 1881
Fragments arranged by size:
7362 1881 1482 1071 1002 465 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 535 2113 3333 3357 3792 13722
Size: 535 1578 1220 24 435 9930
Fragments arranged by size:
9930 1578 1220 535 435 24
BstYI r'GATC_y
Cuts at: 0 515 1025 3776 4376 4439 5858 7385 8435
Size: 515 510 2751 600 63 1419 1527 1050
Cuts at: 8435 12314 12832 13409 13722
Size: 3879 518 577 313
Fragments arranged by size:
3879 2751 1527 1419 1050 600 577 518
515 510 313 63
Bsu36I CC'TnA_GG
Cuts at: 0 794 1915 8286 12245 13722
Size: 794 1121 6371 3959 1477
Fragments arranged by size:
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BtgI C'CryG_G
Cuts at: 0 2153 3366 3861 5210 6728 7988 8111 8513
Size: 2153 1213 495 1349 1518 1260 123 402
Cuts at: 8513 8555 9242 9311 9497 9806 9920 10490 10818
Size: 42 687 69 186 309 114 570 328
Cuts at: 10818 11153 11736 11759 11798 12192 12224 12266 12917
Size: 335 583 23 39 394 32 42 651
Cuts at: 12917 13587 13715 13722
Size: 670 128 7
Fragments arranged by size:
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186 128 123 114 69 42 42 39
32 23 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 308 438 630 3132 3233 7419 9141
Size: 171 137 130 192 2502 101 4186 1722
Cuts at: 9141 11868 12690 13722
Size: 2727 822 1032
Fragments arranged by size:
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137 130 101
BtsI GCAGTG_nn'
Cuts at: 0 63 82 2194 2311 4510 4803 6649 7925
Size: 63 19 2112 117 2199 293 1846 1276
Cuts at: 7925 9329 9866 10146 13722
Size: 1404 537 280 3576
Fragments arranged by size:
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ClaI AT'CG_AT
Cuts at: 0 12695 13722
Size: 12695 1027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1568 1603 12783 12818 13722
Size: 1568 35 11180 35 904
Fragments arranged by size:
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DraIII CAC_nnn'GTG
Cuts at: 0 230 1695 1810 2726 4516 8550 9534 10527
Size: 230 1465 115 916 1790 4034 984 993
Cuts at: 10527 13722
Size: 3195
Fragments arranged by size:
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115
DrdI GACnn_nn'nnGTC
Cuts at: 0 19 805 12646 13182 13722
Size: 19 786 11841 536 540
Fragments arranged by size:
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EaeI y'GGCC_r
Cuts at: 0 297 557 648 977 1205 2432 2510 2813
Size: 297 260 91 329 228 1227 78 303
Cuts at: 2813 3437 3794 3977 4166 4205 5207 5351 5384
Size: 624 357 183 189 39 1002 144 33
Cuts at: 5384 5460 5477 5537 5597 5891 6065 7247 7652
Size: 76 17 60 60 294 174 1182 405
Cuts at: 7652 7727 7965 8387 8427 9239 9647 10259 10911
Size: 75 238 422 40 812 408 612 652
Cuts at: 10911 11492 11691 11993 12098 13187 13230 13662 13722
Size: 581 199 302 105 1089 43 432 60
Fragments arranged by size:
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302 297 294 260 238 228 199 189
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EagI C'GGCC_G
Cuts at: 0 648 977 5460 5891 6065 8387 10911 12098
Size: 648 329 4483 431 174 2322 2524 1187
Cuts at: 12098 13187 13662 13722
Size: 1089 475 60
Fragments arranged by size:
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EarI CTCTTCn'nnn_
Cuts at: 0 661 1441 2746 3008 5038 5507 6049 6224
Size: 661 780 1305 262 2030 469 542 175
Cuts at: 6224 6410 6775 7642 8449 10426 10778 11431 12160
Size: 186 365 867 807 1977 352 653 729
Cuts at: 12160 12298 13638 13722
Size: 138 1340 84
Fragments arranged by size:
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661 653 542 469 365 352 262 186
175 138 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 772 1429 2331 4252 4387 4540 4549 4633
Size: 772 657 902 1921 135 153 9 84
Cuts at: 4633 4729 4738 4822 5038 5101 5131 5197 5524
Size: 96 9 84 216 63 30 66 327
Cuts at: 5524 5587 5650 5677 5704 5920 6025 6241 6343
Size: 63 63 27 27 216 105 216 102
Cuts at: 6343 6373 6394 6550 6736 6925 7081 7168 7324
Size: 30 21 156 186 189 156 87 156
Cuts at: 7324 8046 8479 8626 10456 10603 10732 10966 11112
Size: 722 433 147 1830 147 129 234 146
Cuts at: 11112 11131 11944 12190 13722
Size: 19 813 246 1532
Fragments arranged by size:
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21 19 9 9
EcoICRI GAG'CTC
Cuts at: 0 2103 3045 3453 3684 4364 6768 7344 8124
Size: 2103 942 408 231 680 2404 576 780
Cuts at: 8124 9066 9663 9750 9846 10029 11976 13722
Size: 942 597 87 96 183 1947 1746
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 340 618 660 748 940 990 1066
Size: 56 284 278 42 88 192 50 76
Cuts at: 1066 1138 1234 1246 1273 1576 1651 1945 2031
Size: 72 96 12 27 303 75 294 86
Cuts at: 2031 2095 2101 2254 2380 2482 2716 2851 2887
Size: 64 6 153 126 102 234 135 36
Cuts at: 2887 3088 3301 3322 3355 3496 3865 3916 4207
Size: 201 213 21 33 141 369 51 291
Cuts at: 4207 4714 4801 4876 4993 5182 5296 5467 5740
Size: 507 87 75 117 189 114 171 273
Cuts at: 5740 5818 5890 5932 5938 6001 6124 6451 6685
Size: 78 72 42 6 63 123 327 234
Cuts at: 6685 6736 7204 7228 7402 7465 7648 7828 7903
Size: 51 468 24 174 63 183 180 75
Cuts at: 7903 8209 8290 8518 8704 9061 9193 9232 9460
Size: 306 81 228 186 357 132 39 228
Cuts at: 9460 9649 9978 10186 10267 10495 10545 10681 11272
Size: 189 329 208 81 228 50 136 591
Cuts at: 11272 11560 12001 12049 12205 12229 12463 12607 12852
Size: 288 441 48 156 24 234 144 245
Cuts at: 12852 12967 13036 13222 13264 13492 13722
Size: 115 69 186 42 228 230
Fragments arranged by size:
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306 303 294 291 288 284 278 273
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114 102 96 88 87 86 81 81
78 76 75 75 75 72 72 69
64 63 63 56 51 51 50 50
48 42 42 42 39 36 33 27
24 24 21 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 319 739 2371 2998 3844 4738 4870
Size: 250 69 420 1632 627 846 894 132
Cuts at: 4870 5053 6880 7426 8269 8497 9430 9442 9571
Size: 183 1827 546 843 228 933 12 129
Cuts at: 9571 9976 10246 10423 10474 10843 11038 11101 11569
Size: 405 270 177 51 369 195 63 468
Cuts at: 11569 12157 12208 13204 13306 13722
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Fragments arranged by size:
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69 63 51 51 12
EcoO109I rG'GnC_Cy
Cuts at: 0 324 609 765 1659 1919 2364 2575 2712
Size: 324 285 156 894 260 445 211 137
Cuts at: 2712 3398 3837 4680 4689 6978 7548 8359 8415
Size: 686 439 843 9 2289 570 811 56
Cuts at: 8415 8463 8700 8898 9006 9343 9399 9435 9447
Size: 48 237 198 108 337 56 36 12
Cuts at: 9447 9468 9587 9588 9801 10044 10098 10212 10336
Size: 21 119 1 213 243 54 114 124
Cuts at: 10336 10440 10677 11031 11331 11341 11466 11820 12070
Size: 104 237 354 300 10 125 354 250
Cuts at: 12070 12126 12174 12561 12872 12913 13095 13161 13197
Size: 56 48 387 311 41 182 66 36
Cuts at: 13197 13311 13682 13701 13702 13722
Size: 114 371 19 1 20
Fragments arranged by size:
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114 108 104 66 56 56 56 54
48 48 41 36 36 21 20 19
12 10 9 1 1
EcoRI G'AATT_C
Cuts at: 0 1780 13722
Size: 1780 11942
EcoRV GAT'ATC
Cuts at: 0 525 13722
Size: 525 13197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 5895 8655 10632 11748 12366 13722
Size: 237 5658 2760 1977 1116 618 1356
Fragments arranged by size:
5658 2760 1977 1356 1116 618 237
FseI GG_CCGG'CC
Cuts at: 0 5462 10913 13232 13722
Size: 5462 5451 2319 490
Fragments arranged by size:
5462 5451 2319 490
FspI TGC'GCA
Cuts at: 0 4426 5776 5959 6496 7097 9013 11611 12490
Size: 4426 1350 183 537 601 1916 2598 879
Cuts at: 12490 13722
Size: 1232
Fragments arranged by size:
4426 2598 1916 1350 1232 879 601 537
183
HaeII r_GCGC'y
Cuts at: 0 137 1521 1623 1706 2062 2850 3033 3609
Size: 137 1384 102 83 356 788 183 576
Cuts at: 3609 3642 3743 3861 4479 4575 4734 4863 4980
Size: 33 101 118 618 96 159 129 117
Cuts at: 4980 5005 5205 5676 5763 5869 6030 6103 6150
Size: 25 200 471 87 106 161 73 47
Cuts at: 6150 6873 7220 7233 7296 7422 7602 8691 9096
Size: 723 347 13 63 126 180 1089 405
Cuts at: 9096 9302 10299 11643 11806 12498 12684 13068 13239
Size: 206 997 1344 163 692 186 384 171
Cuts at: 13239 13370 13722
Size: 131 352
Fragments arranged by size:
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576 471 405 384 356 352 347 206
200 186 183 180 171 163 161 159
137 131 129 126 118 117 106 102
101 96 87 83 73 63 47 33
25 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 811 843 5395 5427 6157 6189 9973 10005
Size: 811 32 4552 32 730 32 3784 32
Cuts at: 10005 10171 10203 10384 10416 11040 11072 11104 11136
Size: 166 32 181 32 624 32 32 32
Cuts at: 11136 11575 11607 12118 12150 12491 12523 12658 12690
Size: 439 32 511 32 341 32 135 32
Cuts at: 12690 12772 12804 12940 12972 13722
Size: 82 32 136 32 750
Fragments arranged by size:
4552 3784 811 750 730 624 511 439
341 181 166 136 135 82 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 237 8331 13089 13722
Size: 237 8094 4758 633
Fragments arranged by size:
8094 4758 633 237
HindIII A'AGCT_T
Cuts at: 0 7560 13722
Size: 7560 6162
KasI G'GCGC_C
Cuts at: 0 1619 3739 4730 6026 6146 12680 13366 13722
Size: 1619 2120 991 1296 120 6534 686 356
Fragments arranged by size:
6534 2120 1619 1296 991 686 356 120
KpnI G_GTAC'C
Cuts at: 0 606 13722
Size: 606 13116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1322 4442 7491 9233 9257 9461 10838
Size: 524 798 3120 3049 1742 24 204 1377
Cuts at: 10838 11693 13722
Size: 855 2029
Fragments arranged by size:
3120 3049 2029 1742 1377 855 798 524
204 24
MscI TGG'CCA
Cuts at: 0 299 1207 2434 2815 5353 5386 7654 7729
Size: 299 908 1227 381 2538 33 2268 75
Cuts at: 7729 8429 11494 13722
Size: 700 3065 2228
Fragments arranged by size:
3065 2538 2268 2228 1227 908 700 381
299 75 33
MslI CAynn'nnrTG
Cuts at: 0 269 697 2029 2552 3132 8113 8962 8965
Size: 269 428 1332 523 580 4981 849 3
Cuts at: 8965 9241 10747 13722
Size: 276 1506 2975
Fragments arranged by size:
4981 2975 1506 1332 849 580 523 428
276 269 3
MspA1I CmG'CkG
Cuts at: 0 111 125 166 638 915 1170 1314 1380
Size: 111 14 41 472 277 255 144 66
Cuts at: 1380 1743 2208 2400 2460 2568 2982 3066 3111
Size: 363 465 192 60 108 414 84 45
Cuts at: 3111 3368 3576 3630 3705 3792 3822 3936 4143
Size: 257 208 54 75 87 30 114 207
Cuts at: 4143 4197 4203 4353 4677 4854 4896 5139 5172
Size: 54 6 150 324 177 42 243 33
Cuts at: 5172 5178 5212 5265 5271 5529 5535 5562 5709
Size: 6 34 53 6 258 6 27 147
Cuts at: 5709 5772 5838 5896 5997 6003 6090 6186 6192
Size: 63 66 58 101 6 87 96 6
Cuts at: 6192 6315 6568 6663 6711 6966 6993 7149 7176
Size: 123 253 95 48 255 27 156 27
Cuts at: 7176 7200 7206 7530 7593 7668 7686 7719 7806
Size: 24 6 324 63 75 18 33 87
Cuts at: 7806 7818 7872 7990 8091 8238 8892 9078 9492
Size: 12 54 118 101 147 654 186 414
Cuts at: 9492 9672 9690 9879 9998 10356 10656 10881 10890
Size: 180 18 189 119 358 300 225 9
Cuts at: 10890 11490 11589 11658 11697 11811 11879 12390 12441
Size: 600 99 69 39 114 68 511 51
Cuts at: 12441 12879 13008 13523 13540 13589 13722
Size: 438 129 515 17 49 133
Fragments arranged by size:
654 600 515 511 472 465 438 414
414 363 358 324 324 300 277 258
257 255 255 253 243 225 208 207
192 189 186 180 177 156 150 147
147 144 133 129 123 119 118 114
114 111 108 101 101 99 96 95
87 87 87 84 75 75 69 68
66 66 63 63 60 58 54 54
54 53 51 49 48 45 42 41
39 34 33 33 30 27 27 27
24 18 18 17 14 12 9 6
6 6 6 6 6 6
NaeI GCC'GGC
Cuts at: 0 118 3125 3437 4420 5460 8064 8992 9267
Size: 118 3007 312 983 1040 2604 928 275
Cuts at: 9267 9647 9679 10155 10911 12753 13230 13578 13722
Size: 380 32 476 756 1842 477 348 144
Fragments arranged by size:
3007 2604 1842 1040 983 928 756 477
476 380 348 312 275 144 118 32
NarI GG'CG_CC
Cuts at: 0 1620 3740 4731 6027 6147 12681 13367 13722
Size: 1620 2120 991 1296 120 6534 686 355
Fragments arranged by size:
6534 2120 1620 1296 991 686 355 120
NcoI C'CATG_G
Cuts at: 0 11759 12192 13722
Size: 11759 433 1530
Fragments arranged by size:
11759 1530 433
NgoMIV G'CCGG_C
Cuts at: 0 116 3123 3435 4418 5458 8062 8990 9265
Size: 116 3007 312 983 1040 2604 928 275
Cuts at: 9265 9645 9677 10153 10909 12751 13228 13576 13722
Size: 380 32 476 756 1842 477 348 146
Fragments arranged by size:
3007 2604 1842 1040 983 928 756 477
476 380 348 312 275 146 116 32
NotI GC'GGCC_GC
Cuts at: 0 5891 13722
Size: 5891 7831
NspI r_CATG'y
Cuts at: 0 4272 12786 13722
Size: 4272 8514 936
Fragments arranged by size:
8514 4272 936
PasI CC'CwG_GG
Cuts at: 0 73 13695 13722
Size: 73 13622 27
Fragments arranged by size:
13622 73 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 1116 1170 2571 2715 10893 13722
Size: 1116 54 1401 144 8178 2829
Fragments arranged by size:
8178 2829 1401 1116 144 54
PfoI T'CCnGG_A
Cuts at: 0 155 785 1211 3446 4019 7640 7880 8474
Size: 155 630 426 2235 573 3621 240 594
Cuts at: 8474 11078 11864 11978 12868 12929 13241 13722
Size: 2604 786 114 890 61 312 481
Fragments arranged by size:
3621 2604 2235 890 786 630 594 573
481 426 312 240 155 114 61
PmlI CAC'GTG
Cuts at: 0 1203 2742 4881 13722
Size: 1203 1539 2139 8841
Fragments arranged by size:
8841 2139 1539 1203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 11020 11052 13722
Size: 11020 32 2670
Fragments arranged by size:
11020 2670 32
PpuMI rG'GwC_Cy
Cuts at: 0 324 765 1659 1919 2364 6978 8415 8700
Size: 324 441 894 260 445 4614 1437 285
Cuts at: 8700 8898 9399 9801 10044 10098 10677 11031 12126
Size: 198 501 402 243 54 579 354 1095
Cuts at: 12126 12872 13161 13682 13722
Size: 746 289 521 40
Fragments arranged by size:
4614 1437 1095 894 746 579 521 501
445 441 402 354 324 289 285 260
243 198 54 40
PshAI GACnn'nnGTC
Cuts at: 0 10090 13722
Size: 10090 3632
PspOMI G'GGCC_C
Cuts at: 0 2574 3398 3488 4679 8358 9342 9587 9734
Size: 2574 824 90 1191 3679 984 245 147
Cuts at: 9734 10335 10883 11341 12069 12912 13701 13722
Size: 601 548 458 728 843 789 21
Fragments arranged by size:
3679 2574 1191 984 843 824 789 728
601 548 458 245 147 90 21
PspXI vC'TCGA_Gb
Cuts at: 0 3454 3685 13722
Size: 3454 231 10037
Fragments arranged by size:
10037 3454 231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 694 726 2233 2265 4069 4101 8653 8685
Size: 694 32 1507 32 1804 32 4552 32
Cuts at: 8685 10630 10662 12364 12396 13722
Size: 1945 32 1702 32 1326
Fragments arranged by size:
4552 1945 1804 1702 1507 1326 694 32
32 32 32 32 32
PstI C_TGCA'G
Cuts at: 0 383 914 1286 1340 1365 1607 1658 1880
Size: 383 531 372 54 25 242 51 222
Cuts at: 1880 2183 2306 2375 2965 2990 3065 3572 3752
Size: 303 123 69 590 25 75 507 180
Cuts at: 3752 3827 4262 4349 4460 4508 4688 4712 4817
Size: 75 435 87 111 48 180 24 105
Cuts at: 4817 4871 5021 5300 5316 6191 6224 6290 6486
Size: 54 150 279 16 875 33 66 196
Cuts at: 6486 6662 6710 6881 7079 7121 7490 7538 7556
Size: 176 48 171 198 42 369 48 18
Cuts at: 7556 7802 7877 8057 8090 8159 8231 8678 9083
Size: 246 75 180 33 69 72 447 405
Cuts at: 9083 9767 10136 11810 12389 13319 13722
Size: 684 369 1674 579 930 403
Fragments arranged by size:
1674 930 875 684 590 579 531 507
447 435 405 403 383 372 369 369
303 279 246 242 222 198 196 180
180 180 176 171 150 123 111 105
87 75 75 75 72 69 69 66
54 54 51 48 48 48 42 33
33 25 25 24 18 16
PvuII CAG'CTG
Cuts at: 0 166 638 915 1314 1743 2208 2400 2460
Size: 166 472 277 399 429 465 192 60
Cuts at: 2460 2568 2982 3066 3630 3705 3822 3936 4203
Size: 108 414 84 564 75 117 114 267
Cuts at: 4203 4353 4854 4896 5178 5271 5535 5772 6003
Size: 150 501 42 282 93 264 237 231
Cuts at: 6003 6186 6192 6315 6568 6663 6966 7149 7206
Size: 183 6 123 253 95 303 183 57
Cuts at: 7206 7530 7668 7806 7872 8238 9078 9492 9690
Size: 324 138 138 66 366 840 414 198
Cuts at: 9690 10356 10656 10890 11490 11589 11658 11697 11811
Size: 666 300 234 600 99 69 39 114
Cuts at: 11811 11879 12390 12879 13722
Size: 68 511 489 843
Fragments arranged by size:
843 840 666 600 564 511 501 489
472 465 429 414 414 399 366 324
303 300 282 277 267 264 253 237
234 231 198 192 183 183 166 150
138 138 123 117 114 114 108 99
95 93 84 75 69 68 66 60
57 42 39 6
SacI G_AGCT'C
Cuts at: 0 2105 3047 3455 3686 4366 6770 7346 8126
Size: 2105 942 408 231 680 2404 576 780
Cuts at: 8126 9068 9665 9752 9848 10031 11978 13722
Size: 942 597 87 96 183 1947 1744
Fragments arranged by size:
2404 2105 1947 1744 942 942 780 680
597 576 408 231 183 96 87
SacII CC_GC'GG
Cuts at: 0 3369 5213 7991 13590 13722
Size: 3369 1844 2778 5599 132
Fragments arranged by size:
5599 3369 2778 1844 132
SanDI GG'GwC_CC
Cuts at: 0 9399 13722
Size: 9399 4323
SapI GCTCTTCn'nnn_
Cuts at: 0 1441 2746 5038 6049 6775 7642 11431 13722
Size: 1441 1305 2292 1011 726 867 3789 2291
Fragments arranged by size:
3789 2292 2291 1441 1305 1011 867 726
SbfI CC_TGCA'GG
Cuts at: 0 1658 4688 4871 6881 8159 13722
Size: 1658 3030 183 2010 1278 5563
Fragments arranged by size:
5563 3030 2010 1658 1278 183
ScaI AGT'ACT
Cuts at: 0 9862 13722
Size: 9862 3860
SexAI A'CCwGG_T
Cuts at: 0 12983 13722
Size: 12983 739
SfcI C'TryA_G
Cuts at: 0 379 910 1282 1336 1361 1603 1654 1876
Size: 379 531 372 54 25 242 51 222
Cuts at: 1876 2179 2203 2302 2371 2961 2986 3061 3568
Size: 303 24 99 69 590 25 75 507
Cuts at: 3568 3748 3823 4047 4258 4345 4456 4504 4684
Size: 180 75 224 211 87 111 48 180
Cuts at: 4684 4708 4813 4867 5017 5296 5312 6187 6220
Size: 24 105 54 150 279 16 875 33
Cuts at: 6220 6274 6286 6482 6658 6706 6877 7075 7117
Size: 54 12 196 176 48 171 198 42
Cuts at: 7117 7486 7534 7552 7588 7798 7873 8053 8086
Size: 369 48 18 36 210 75 180 33
Cuts at: 8086 8155 8227 8674 9079 9621 9763 10132 10386
Size: 69 72 447 405 542 142 369 254
Cuts at: 10386 11806 12385 13315 13503 13512 13722
Size: 1420 579 930 188 9 210
Fragments arranged by size:
1420 930 875 590 579 542 531 507
447 405 379 372 369 369 303 279
254 242 224 222 211 210 210 198
196 188 180 180 180 176 171 150
142 111 105 99 87 75 75 75
72 69 69 54 54 54 51 48
48 48 42 36 33 33 25 25
24 24 18 16 12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2440 2680 3436 3466 3976 4687 5485 6202
Size: 2440 240 756 30 510 711 798 717
Cuts at: 6202 6733 9341 13141 13195 13722
Size: 531 2608 3800 54 527
Fragments arranged by size:
3800 2608 2440 798 756 717 711 531
527 510 240 54 30
SfoI GGC'GCC
Cuts at: 0 1621 3741 4732 6028 6148 12682 13368 13722
Size: 1621 2120 991 1296 120 6534 686 354
Fragments arranged by size:
6534 2120 1621 1296 991 686 354 120
SgrAI Cr'CCGG_yG
Cuts at: 0 11497 12799 13057 13722
Size: 11497 1302 258 665
Fragments arranged by size:
11497 1302 665 258
SmaI CCC'GGG
Cuts at: 0 349 1840 6370 6463 8785 9460 13573 13722
Size: 349 1491 4530 93 2322 675 4113 149
Fragments arranged by size:
4530 4113 2322 1491 675 349 149 93
SmlI C'TyrA_G
Cuts at: 0 1144 1456 3343 3415 3454 3634 3685 3958
Size: 1144 312 1887 72 39 180 51 273
Cuts at: 3958 4987 5623 5647 6808 7015 7054 7234 7492
Size: 1029 636 24 1161 207 39 180 258
Cuts at: 7492 10072 11059 11110 11209 13405 13722
Size: 2580 987 51 99 2196 317
Fragments arranged by size:
2580 2196 1887 1161 1144 1029 987 636
317 312 273 258 207 180 180 99
72 51 51 39 39 24
SphI G_CATG'C
Cuts at: 0 4272 13722
Size: 4272 9450
SrfI GCCC'GGGC
Cuts at: 0 6463 13722
Size: 6463 7259
StuI AGG'CCT
Cuts at: 0 745 1760 2429 2884 3073 3469 5701 9619
Size: 745 1015 669 455 189 396 2232 3918
Cuts at: 9619 12550 13027 13144 13722
Size: 2931 477 117 578
Fragments arranged by size:
3918 2931 2232 1015 745 669 578 477
455 396 189 117
StyI C'CwwG_G
Cuts at: 0 96 480 560 1073 1097 1904 2444 3464
Size: 96 384 80 513 24 807 540 1020
Cuts at: 3464 3734 3989 4385 5354 6128 6998 7655 8630
Size: 270 255 396 969 774 870 657 975
Cuts at: 8630 8747 9601 9614 10175 10607 10922 11759 11909
Size: 117 854 13 561 432 315 837 150
Cuts at: 11909 12192 12341 13493 13722
Size: 283 149 1152 229
Fragments arranged by size:
1152 1020 975 969 870 854 837 807
774 657 561 540 513 432 396 384
315 283 270 255 229 150 149 117
96 80 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1662 2489 3679 6688 7366 9799 12337 13722
Size: 1662 827 1190 3009 678 2433 2538 1385
Fragments arranged by size:
3009 2538 2433 1662 1385 1190 827 678
TatI w'GTAC_w
Cuts at: 0 1541 2408 3902 4076 9860 10751 13722
Size: 1541 867 1494 174 5784 891 2971
Fragments arranged by size:
5784 2971 1541 1494 891 867 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1954 1986 7370 7402 10810 10842
Size: 1171 32 751 32 5384 32 3408 32
Cuts at: 10842 11085 11117 12595 12627 13722
Size: 243 32 1478 32 1095
Fragments arranged by size:
5384 3408 1478 1171 1095 751 243 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 13084 13722
Size: 13084 638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1284 5442 7440 10029 10269 11766 12876 13722
Size: 1284 4158 1998 2589 240 1497 1110 846
Fragments arranged by size:
4158 2589 1998 1497 1284 1110 846 240
XhoI C'TCGA_G
Cuts at: 0 3454 3685 11059 11110 13722
Size: 3454 231 7374 51 2612
Fragments arranged by size:
7374 3454 2612 231 51
XmaI C'CCGG_G
Cuts at: 0 347 1838 6368 6461 8783 9458 13571 13722
Size: 347 1491 4530 93 2322 675 4113 151
Fragments arranged by size:
4530 4113 2322 1491 675 347 151 93
XmnI GAAnn'nnTTC
Cuts at: 0 220 13144 13722
Size: 220 12924 578
Fragments arranged by size:
12924 578 220
ZraI GAC'GTC
Cuts at: 0 8790 12470 13722
Size: 8790 3680 1252
Fragments arranged by size:
8790 3680 1252
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BmtI BsrDI BstBI BstZ17I DraI FspAI HpaI
MfeI MluI NdeI NheI NruI NsiI PacI PciI
PmeI PsiI PvuI RsrII SalI SgfI SnaBI SpeI
SspI SwaI XbaI