Restriction map for
pF1KE2521
(Linear) (Six-base) MAPSORT of: KIEE2521.seq Check: 5422 from: 1 to: 1653
KIEE2521.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AgeI A'CCGG_T
Cuts at: 0 1233 1653
Size: 1233 420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 1653
Size: 227 32 1394
Fragments arranged by size:
1394 227 32
ApoI r'AATT_y
Cuts at: 0 1146 1503 1653
Size: 1146 357 150
Fragments arranged by size:
1146 357 150
BamHI G'GATC_C
Cuts at: 0 1596 1653
Size: 1596 57
BanI G'GyrC_C
Cuts at: 0 688 702 1653
Size: 688 14 951
Fragments arranged by size:
951 688 14
BanII G_rGCy'C
Cuts at: 0 1111 1653
Size: 1111 542
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 104 359 559 569 1277 1653
Size: 33 71 255 200 10 708 376
Fragments arranged by size:
708 376 255 200 71 33 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1050 1084 1653
Size: 1050 34 569
Fragments arranged by size:
1050 569 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 253 1653
Size: 253 1400
BglII A'GATC_T
Cuts at: 0 270 364 1653
Size: 270 94 1289
Fragments arranged by size:
1289 270 94
BlpI GC'TnA_GC
Cuts at: 0 11 1105 1325 1653
Size: 11 1094 220 328
Fragments arranged by size:
1094 328 220 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 889 1653
Size: 889 764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 1653
Size: 765 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 578 1196 1601 1653
Size: 16 562 618 405 52
Fragments arranged by size:
618 562 405 52 16
Bpu10I CC'TnA_GC
Cuts at: 0 1536 1653
Size: 1536 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 274 1653
Size: 156 118 1379
Fragments arranged by size:
1379 156 118
BsaWI w'CCGG_w
Cuts at: 0 1233 1653
Size: 1233 420
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1363 1366 1369 1653
Size: 1363 3 3 284
Fragments arranged by size:
1363 284 3 3
BseYI C'CCAG_C
Cuts at: 0 1635 1653
Size: 1635 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1653
Size: 695 958
Bsp1286I G_dGCh'C
Cuts at: 0 1111 1653
Size: 1111 542
BspHI T'CATG_A
Cuts at: 0 771 1470 1653
Size: 771 699 183
Fragments arranged by size:
771 699 183
BsrBI CCG'CTC
Cuts at: 0 342 1653
Size: 342 1311
BsrDI GCAATG_nn'
Cuts at: 0 924 1653
Size: 924 729
BsrFI r'CCGG_y
Cuts at: 0 1233 1653
Size: 1233 420
BstEII G'GTnAC_C
Cuts at: 0 828 1653
Size: 828 825
BstYI r'GATC_y
Cuts at: 0 221 263 270 364 1596 1653
Size: 221 42 7 94 1232 57
Fragments arranged by size:
1232 221 94 57 42 7
Bsu36I CC'TnA_GG
Cuts at: 0 247 1653
Size: 247 1406
BtgI C'CryG_G
Cuts at: 0 524 1653
Size: 524 1129
DraI TTT'AAA
Cuts at: 0 320 606 1653
Size: 320 286 1047
Fragments arranged by size:
1047 320 286
EarI CTCTTCn'nnn_
Cuts at: 0 179 302 392 1004 1653
Size: 179 123 90 612 649
Fragments arranged by size:
649 612 179 123 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 959 1653
Size: 959 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 578 1196 1601 1653
Size: 16 562 618 405 52
Fragments arranged by size:
618 562 405 52 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 798 1653
Size: 798 855
EcoRI G'AATT_C
Cuts at: 0 1146 1653
Size: 1146 507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1033 1653
Size: 1033 620
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 1072 1104 1178 1210 1366 1398
Size: 255 32 785 32 74 32 156 32
Cuts at: 1398 1581 1585 1613 1617 1653
Size: 183 4 28 4 36
Fragments arranged by size:
785 255 183 156 74 36 32 32
32 32 28 4 4
HindIII A'AGCT_T
Cuts at: 0 512 1015 1533 1653
Size: 512 503 518 120
Fragments arranged by size:
518 512 503 120
MfeI C'AATT_G
Cuts at: 0 335 404 1653
Size: 335 69 1249
Fragments arranged by size:
1249 335 69
MspA1I CmG'CkG
Cuts at: 0 170 1653
Size: 170 1483
NcoI C'CATG_G
Cuts at: 0 524 1653
Size: 524 1129
PsiI TTA'TAA
Cuts at: 0 361 1653
Size: 361 1292
SmlI C'TyrA_G
Cuts at: 0 135 253 1653
Size: 135 118 1400
Fragments arranged by size:
1400 135 118
SspI AAT'ATT
Cuts at: 0 1124 1653
Size: 1124 529
StuI AGG'CCT
Cuts at: 0 133 804 1653
Size: 133 671 849
Fragments arranged by size:
849 671 133
StyI C'CwwG_G
Cuts at: 0 524 1048 1099 1653
Size: 524 524 51 554
Fragments arranged by size:
554 524 524 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1170 1653
Size: 1170 483
TatI w'GTAC_w
Cuts at: 0 565 1653
Size: 565 1088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 1631 1653
Size: 1489 32 110 22
Fragments arranged by size:
1489 110 32 22
XbaI T'CTAG_A
Cuts at: 0 153 231 1653
Size: 153 78 1422
Fragments arranged by size:
1422 153 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 1653
Size: 909 744
Enzymes that do cut:
AgeI AloI ApoI BamHI BanI BanII BbsI BcgI
BciVI BglII BlpI BmrI BplI BpmI Bpu10I BpuEI
BsaWI BseRI BseYI BsgI Bsp1286I BspHI BsrBI BsrDI
BsrFI BstEII BstYI Bsu36I BtgI DraI EarI EciI
Eco57MI EcoNI EcoRI FalI Hin4I HindIII MfeI MspA1I
NcoI PsiI SmlI SspI StuI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BclI
BfrBI BglI Bme1580I BmgBI BmtI BsaI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XhoI XmaI XmnI
ZraI