Restriction map for
pF1KE2532
(Linear) (Six-base) MAPSORT of: KIEE2532.seq Check: 6657 from: 1 to: 5781
KIEE2532.orf
With 182 enzymes: SgfI *
August 6, 2014 11:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2101 5781
Size: 2101 3680
AatII G_ACGT'C
Cuts at: 0 1319 3608 5781
Size: 1319 2289 2173
Fragments arranged by size:
2289 2173 1319
AccI GT'mk_AC
Cuts at: 0 2315 5781
Size: 2315 3466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 358 498 689 876 1003 1169 2448
Size: 210 148 140 191 187 127 166 1279
Cuts at: 2448 5169 5185 5781
Size: 2721 16 596
Fragments arranged by size:
2721 1279 596 210 191 187 166 148
140 127 16
AfeI AGC'GCT
Cuts at: 0 1864 5722 5781
Size: 1864 3858 59
Fragments arranged by size:
3858 1864 59
AflII C'TTAA_G
Cuts at: 0 2307 5781
Size: 2307 3474
AflIII A'CryG_T
Cuts at: 0 179 2133 3317 3620 4220 5781
Size: 179 1954 1184 303 600 1561
Fragments arranged by size:
1954 1561 1184 600 303 179
AhdI GACnn_n'nnGTC
Cuts at: 0 66 5781
Size: 66 5715
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4641 4673 5590 5622 5781
Size: 4641 32 917 32 159
Fragments arranged by size:
4641 917 159 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 201 789 1509 2019 2792 4159 4323 4575
Size: 201 588 720 510 773 1367 164 252
Cuts at: 4575 4802 5149 5610 5781
Size: 227 347 461 171
Fragments arranged by size:
1367 773 720 588 510 461 347 252
227 201 171 164
ApoI r'AATT_y
Cuts at: 0 78 3232 4984 5781
Size: 78 3154 1752 797
Fragments arranged by size:
3154 1752 797 78
AseI AT'TA_AT
Cuts at: 0 947 5781
Size: 947 4834
AvaI C'yCGr_G
Cuts at: 0 1990 5781
Size: 1990 3791
BamHI G'GATC_C
Cuts at: 0 1233 4934 5192 5465 5781
Size: 1233 3701 258 273 316
Fragments arranged by size:
3701 1233 316 273 258
BanI G'GyrC_C
Cuts at: 0 1165 1654 1745 2181 2625 3264 5017 5626
Size: 1165 489 91 436 444 639 1753 609
Cuts at: 5626 5781
Size: 155
Fragments arranged by size:
1753 1165 639 609 489 444 436 155
91
BanII G_rGCy'C
Cuts at: 0 686 1344 1472 3197 3392 4616 5767 5781
Size: 686 658 128 1725 195 1224 1151 14
Fragments arranged by size:
1725 1224 1151 686 658 195 128 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 2369 3600 3686 4933 5781
Size: 2369 1231 86 1247 848
Fragments arranged by size:
2369 1247 1231 848 86
BbvCI CC'TCA_GC
Cuts at: 0 3698 4298 4727 5781
Size: 3698 600 429 1054
Fragments arranged by size:
3698 1054 600 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 455 610 644 5117 5151 5781
Size: 421 34 155 34 4473 34 630
Fragments arranged by size:
4473 630 421 155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1131 2226 3846 5356 5781
Size: 1131 1095 1620 1510 425
Fragments arranged by size:
1620 1510 1131 1095 425
BclI T'GATC_A
Cuts at: 0 533 3008 5781
Size: 533 2475 2773
Fragments arranged by size:
2773 2475 533
BlpI GC'TnA_GC
Cuts at: 0 3364 5781
Size: 3364 2417
Bme1580I G_kGCm'C
Cuts at: 0 282 1394 1787 2186 2630 3267 5781
Size: 282 1112 393 399 444 637 2514
Fragments arranged by size:
2514 1112 637 444 399 393 282
BmgBI CAC'GTC
Cuts at: 0 2457 4269 5781
Size: 2457 1812 1512
Fragments arranged by size:
2457 1812 1512
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1500 1821 2966 3390 4488 4812 5781
Size: 72 1428 321 1145 424 1098 324 969
Fragments arranged by size:
1428 1145 1098 969 424 324 321 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2646 5781
Size: 2646 3135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1297 1814 2646 2864 3064 3615 4167
Size: 559 738 517 832 218 200 551 552
Cuts at: 4167 4643 5781
Size: 476 1138
Fragments arranged by size:
1138 832 738 559 552 551 517 476
218 200
Bpu10I CC'TnA_GC
Cuts at: 0 1343 3225 3698 4298 4727 5781
Size: 1343 1882 473 600 429 1054
Fragments arranged by size:
1882 1343 1054 600 473 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 343 1708 2332 5781
Size: 334 9 1365 624 3449
Fragments arranged by size:
3449 1365 624 334 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 139 1039 2945 3953 4364 4519 5781
Size: 137 2 900 1906 1008 411 155 1262
Fragments arranged by size:
1906 1262 1008 900 411 155 137 2
BsaAI yAC'GTr
Cuts at: 0 1788 2136 3320 3623 4419 5781
Size: 1788 348 1184 303 796 1362
Fragments arranged by size:
1788 1362 1184 796 348 303
BsaBI GATnn'nnATC
Cuts at: 0 2882 4783 5781
Size: 2882 1901 998
Fragments arranged by size:
2882 1901 998
BsaHI Gr'CG_yC
Cuts at: 0 1316 3605 5173 5781
Size: 1316 2289 1568 608
Fragments arranged by size:
2289 1568 1316 608
BsaWI w'CCGG_w
Cuts at: 0 422 5781
Size: 422 5359
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 844 874 1342 1372 2575 2605
Size: 272 30 542 30 468 30 1203 30
Cuts at: 2605 3655 3685 4096 4126 4481 4511 4571 4601
Size: 1050 30 411 30 355 30 60 30
Cuts at: 4601 4641 4671 5137 5167 5590 5620 5781
Size: 40 30 466 30 423 30 161
Fragments arranged by size:
1203 1050 542 468 466 423 411 355
272 161 60 40 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 337 1047 3715 3718 5019 5126 5611
Size: 277 60 710 2668 3 1301 107 485
Cuts at: 5611 5781
Size: 170
Fragments arranged by size:
2668 1301 710 485 277 170 107 60
3
BseYI C'CCAG_C
Cuts at: 0 50 291 876 1199 1405 1881 1923 2785
Size: 50 241 585 323 206 476 42 862
Cuts at: 2785 3095 3260 4514 4575 5258 5781
Size: 310 165 1254 61 683 523
Fragments arranged by size:
1254 862 683 585 523 476 323 310
241 206 165 61 50 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 349 350 376 2434 3661 4172 4555
Size: 103 246 1 26 2058 1227 511 383
Cuts at: 4555 5359 5623 5781
Size: 804 264 158
Fragments arranged by size:
2058 1227 804 511 383 264 246 158
103 26 1
BsiEI CG_ry'CG
Cuts at: 0 728 3602 5781
Size: 728 2874 2179
Fragments arranged by size:
2874 2179 728
BsiHKAI G_wGCw'C
Cuts at: 0 686 929 1969 2348 4565 5781
Size: 686 243 1040 379 2217 1216
Fragments arranged by size:
2217 1216 1040 686 379 243
BsiWI C'GTAC_G
Cuts at: 0 3320 5781
Size: 3320 2461
BsmI GAATG_Cn'
Cuts at: 0 763 3440 5781
Size: 763 2677 2341
Fragments arranged by size:
2677 2341 763
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4259 5781
Size: 4259 1522
Bsp1286I G_dGCh'C
Cuts at: 0 282 686 929 1344 1394 1472 1787 1969
Size: 282 404 243 415 50 78 315 182
Cuts at: 1969 2186 2348 2630 3197 3267 3392 4565 4616
Size: 217 162 282 567 70 125 1173 51
Cuts at: 4616 5767 5781
Size: 1151 14
Fragments arranged by size:
1173 1151 567 415 404 315 282 282
243 217 182 162 125 78 70 51
50 14
BspEI T'CCGG_A
Cuts at: 0 422 5781
Size: 422 5359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1505 2101 3074 5781
Size: 1505 596 973 2707
Fragments arranged by size:
2707 1505 973 596
BsrDI GCAATG_nn'
Cuts at: 0 2387 2829 3737 4622 5517 5781
Size: 2387 442 908 885 895 264
Fragments arranged by size:
2387 908 895 885 442 264
BsrFI r'CCGG_y
Cuts at: 0 338 1657 5781
Size: 338 1319 4124
Fragments arranged by size:
4124 1319 338
BsrGI T'GTAC_A
Cuts at: 0 1349 4742 5781
Size: 1349 3393 1039
Fragments arranged by size:
3393 1349 1039
BssSI C'ACGA_G
Cuts at: 0 1042 5136 5781
Size: 1042 4094 645
Fragments arranged by size:
4094 1042 645
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1878 4159 5781
Size: 1878 2281 1622
Fragments arranged by size:
2281 1878 1622
BstBI TT'CG_AA
Cuts at: 0 2414 2678 5781
Size: 2414 264 3103
Fragments arranged by size:
3103 2414 264
BstEII G'GTnAC_C
Cuts at: 0 1611 4308 4464 5781
Size: 1611 2697 156 1317
Fragments arranged by size:
2697 1611 1317 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2443 3157 3204 3417 4639 5781
Size: 2443 714 47 213 1222 1142
Fragments arranged by size:
2443 1222 1142 714 213 47
BstYI r'GATC_y
Cuts at: 0 500 1233 1376 1943 4302 4818 4934 5192
Size: 500 733 143 567 2359 516 116 258
Cuts at: 5192 5294 5465 5781
Size: 102 171 316
Fragments arranged by size:
2359 733 567 516 500 316 258 171
143 116 102
Bsu36I CC'TnA_GG
Cuts at: 0 1502 1841 1933 4133 5781
Size: 1502 339 92 2200 1648
Fragments arranged by size:
2200 1648 1502 339 92
BtgI C'CryG_G
Cuts at: 0 380 1049 1385 1472 1627 2697 3041 3725
Size: 380 669 336 87 155 1070 344 684
Cuts at: 3725 5781
Size: 2056
Fragments arranged by size:
2056 1070 684 669 380 344 336 155
87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1750 2835 5109 5395 5781
Size: 1750 1085 2274 286 386
Fragments arranged by size:
2274 1750 1085 386 286
BtsI GCAGTG_nn'
Cuts at: 0 199 337 4435 4682 4803 5081 5285 5781
Size: 199 138 4098 247 121 278 204 496
Fragments arranged by size:
4098 496 278 247 204 199 138 121
ClaI AT'CG_AT
Cuts at: 0 4430 5781
Size: 4430 1351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 111 146 5781
Size: 111 35 5635
Fragments arranged by size:
5635 111 35
EaeI y'GGCC_r
Cuts at: 0 1283 1475 1659 3599 4097 4442 4701 5140
Size: 1283 192 184 1940 498 345 259 439
Cuts at: 5140 5781
Size: 641
Fragments arranged by size:
1940 1283 641 498 439 345 259 192
184
EagI C'GGCC_G
Cuts at: 0 3599 5781
Size: 3599 2182
EarI CTCTTCn'nnn_
Cuts at: 0 454 895 2742 3569 3574 3779 4570 4996
Size: 454 441 1847 827 5 205 791 426
Cuts at: 4996 5097 5781
Size: 101 684
Fragments arranged by size:
1847 827 791 684 454 441 426 205
101 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 656 1129 4041 5781
Size: 656 473 2912 1740
Fragments arranged by size:
2912 1740 656 473
EcoICRI GAG'CTC
Cuts at: 0 684 5781
Size: 684 5097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 358 498 559 689 876 1003 1169
Size: 210 148 140 61 130 187 127 166
Cuts at: 1169 1297 1814 2448 2646 2864 3064 3615 4167
Size: 128 517 634 198 218 200 551 552
Cuts at: 4167 4643 5169 5185 5781
Size: 476 526 16 596
Fragments arranged by size:
634 596 552 551 526 517 476 218
210 200 198 187 166 148 140 130
128 127 61 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1501 2545 3070 4053 5781
Size: 1501 1044 525 983 1728
Fragments arranged by size:
1728 1501 1044 983 525
EcoO109I rG'GnC_Cy
Cuts at: 0 354 489 1244 1494 1506 1845 1937 2419
Size: 354 135 755 250 12 339 92 482
Cuts at: 2419 2782 3063 3075 3252 3405 3708 4482 5536
Size: 363 281 12 177 153 303 774 1054
Cuts at: 5536 5781
Size: 245
Fragments arranged by size:
1054 774 755 482 363 354 339 303
281 250 245 177 153 135 92 12
12
EcoRV GAT'ATC
Cuts at: 0 675 3087 5781
Size: 675 2412 2694
Fragments arranged by size:
2694 2412 675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 647 1007 5781
Size: 647 360 4774
Fragments arranged by size:
4774 647 360
HaeII r_GCGC'y
Cuts at: 0 1866 5724 5781
Size: 1866 3858 57
Fragments arranged by size:
3858 1866 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 721 753 844 876 3379 3411
Size: 52 32 637 32 91 32 2503 32
Cuts at: 3411 3923 3955 5137 5169 5409 5441 5561 5593
Size: 512 32 1182 32 240 32 120 32
Cuts at: 5593 5781
Size: 188
Fragments arranged by size:
2503 1182 637 512 240 188 120 91
52 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2460 5781
Size: 2460 3321
HindIII A'AGCT_T
Cuts at: 0 3561 5781
Size: 3561 2220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1971 5781
Size: 1971 3810
MscI TGG'CCA
Cuts at: 0 1285 1477 4099 4703 5142 5781
Size: 1285 192 2622 604 439 639
Fragments arranged by size:
2622 1285 639 604 439 192
MslI CAynn'nnrTG
Cuts at: 0 3730 5781
Size: 3730 2051
MspA1I CmG'CkG
Cuts at: 0 94 2439 2874 3427 4539 5262 5781
Size: 94 2345 435 553 1112 723 519
Fragments arranged by size:
2345 1112 723 553 519 435 94
NcoI C'CATG_G
Cuts at: 0 380 1385 1472 3041 5781
Size: 380 1005 87 1569 2740
Fragments arranged by size:
2740 1569 1005 380 87
NspI r_CATG'y
Cuts at: 0 19 183 990 2271 4092 5781
Size: 19 164 807 1281 1821 1689
Fragments arranged by size:
1821 1689 1281 807 164 19
PasI CC'CwG_GG
Cuts at: 0 2186 3241 3450 3749 5781
Size: 2186 1055 209 299 2032
Fragments arranged by size:
2186 2032 1055 299 209
PciI A'CATG_T
Cuts at: 0 179 5781
Size: 179 5602
PflMI CCAn_nnn'nTGG
Cuts at: 0 1367 1742 1977 2792 3506 3693 4355 4575
Size: 1367 375 235 815 714 187 662 220
Cuts at: 4575 5149 5781
Size: 574 632
Fragments arranged by size:
1367 815 714 662 632 574 375 235
220 187
PfoI T'CCnGG_A
Cuts at: 0 677 1372 1556 3080 4659 5781
Size: 677 695 184 1524 1579 1122
Fragments arranged by size:
1579 1524 1122 695 677 184
PmlI CAC'GTG
Cuts at: 0 1788 2136 5781
Size: 1788 348 3645
Fragments arranged by size:
3645 1788 348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2225 2257 3568 3600 4641 4673 5590 5622
Size: 2225 32 1311 32 1041 32 917 32
Cuts at: 5622 5781
Size: 159
Fragments arranged by size:
2225 1311 1041 917 159 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 489 1494 1845 1937 3063 3252 3405 3708
Size: 489 1005 351 92 1126 189 153 303
Cuts at: 3708 4482 5781
Size: 774 1299
Fragments arranged by size:
1299 1126 1005 774 489 351 303 189
153 92
PsiI TTA'TAA
Cuts at: 0 260 5781
Size: 260 5521
PstI C_TGCA'G
Cuts at: 0 1136 1514 3443 3536 4319 4959 5781
Size: 1136 378 1929 93 783 640 822
Fragments arranged by size:
1929 1136 822 783 640 378 93
PvuII CAG'CTG
Cuts at: 0 2874 5781
Size: 2874 2907
SacI G_AGCT'C
Cuts at: 0 686 5781
Size: 686 5095
SanDI GG'GwC_CC
Cuts at: 0 3405 5781
Size: 3405 2376
SapI GCTCTTCn'nnn_
Cuts at: 0 895 4570 5781
Size: 895 3675 1211
Fragments arranged by size:
3675 1211 895
SbfI CC_TGCA'GG
Cuts at: 0 1136 1514 5781
Size: 1136 378 4267
Fragments arranged by size:
4267 1136 378
ScaI AGT'ACT
Cuts at: 0 5713 5781
Size: 5713 68
SexAI A'CCwGG_T
Cuts at: 0 4384 5781
Size: 4384 1397
SfcI C'TryA_G
Cuts at: 0 1132 1450 1510 3439 3532 3906 4315 4955
Size: 1132 318 60 1929 93 374 409 640
Cuts at: 4955 5442 5531 5781
Size: 487 89 250
Fragments arranged by size:
1929 1132 640 487 409 374 318 250
93 89 60
SmlI C'TyrA_G
Cuts at: 0 349 358 1723 2307 2347 5781
Size: 349 9 1365 584 40 3434
Fragments arranged by size:
3434 1365 584 349 40 9
SphI G_CATG'C
Cuts at: 0 2271 4092 5781
Size: 2271 1821 1689
Fragments arranged by size:
2271 1821 1689
StuI AGG'CCT
Cuts at: 0 1839 2917 4032 4677 4732 4961 5781
Size: 1839 1078 1115 645 55 229 820
Fragments arranged by size:
1839 1115 1078 820 645 229 55
StyI C'CwwG_G
Cuts at: 0 380 1385 1472 2546 2633 3041 5461 5781
Size: 380 1005 87 1074 87 408 2420 320
Fragments arranged by size:
2420 1074 1005 408 380 320 87 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 319 714 1599 1620 3446 3669 5781
Size: 268 51 395 885 21 1826 223 2112
Fragments arranged by size:
2112 1826 885 395 268 223 51 21
TatI w'GTAC_w
Cuts at: 0 1349 3373 4742 5711 5781
Size: 1349 2024 1369 969 70
Fragments arranged by size:
2024 1369 1349 969 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 81 113 272 294 304 326 3389 3421
Size: 81 32 159 22 10 22 3063 32
Cuts at: 3421 3510 3542 3700 3732 4481 4513 4571 4603
Size: 89 32 158 32 749 32 58 32
Cuts at: 4603 5781
Size: 1178
Fragments arranged by size:
3063 1178 749 159 158 89 81 58
32 32 32 32 32 32 22 22
10
Tth111I GACn'n_nGTC
Cuts at: 0 724 5781
Size: 724 5057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4266 5781
Size: 4266 1515
XmnI GAAnn'nnTTC
Cuts at: 0 826 2536 3583 5781
Size: 826 1710 1047 2198
Fragments arranged by size:
2198 1710 1047 826
ZraI GAC'GTC
Cuts at: 0 1317 3606 5781
Size: 1317 2289 2175
Fragments arranged by size:
2289 2175 1317
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AloI AlwNI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PsiI PstI PvuII SacI SanDI SapI SbfI ScaI
SexAI SfcI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AleI AlfI ApaI ApaLI AscI
AsiSI AvrII BaeI BbeI BfrBI BglI BglII BmtI
BspHI BsrBI BssHII BstZ17I DraI DraIII DrdI EcoRI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmaI