Restriction map for
pF1KE2533
(Linear) (Six-base) MAPSORT of: KIEE2533.seq Check: 4269 from: 1 to: 1578
KIEE2533.orf
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 804 1578
Size: 804 774
AccI GT'mk_AC
Cuts at: 0 419 1578
Size: 419 1159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1505 1578
Size: 716 789 73
Fragments arranged by size:
789 716 73
AlwNI CAG_nnn'CTG
Cuts at: 0 916 1578
Size: 916 662
ApaI G_GGCC'C
Cuts at: 0 278 1578
Size: 278 1300
BamHI G'GATC_C
Cuts at: 0 883 1578
Size: 883 695
BanI G'GyrC_C
Cuts at: 0 5 1578
Size: 5 1573
BanII G_rGCy'C
Cuts at: 0 278 851 1578
Size: 278 573 727
Fragments arranged by size:
727 573 278
BbsI GAAGACnn'nnnn_
Cuts at: 0 1185 1372 1578
Size: 1185 187 206
Fragments arranged by size:
1185 206 187
BbvCI CC'TCA_GC
Cuts at: 0 763 1141 1578
Size: 763 378 437
Fragments arranged by size:
763 437 378
BfrBI ATG'CAT
Cuts at: 0 313 1578
Size: 313 1265
BglII A'GATC_T
Cuts at: 0 381 1578
Size: 381 1197
Bme1580I G_kGCm'C
Cuts at: 0 8 278 1578
Size: 8 270 1300
Fragments arranged by size:
1300 270 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 676 1578
Size: 652 24 902
Fragments arranged by size:
902 652 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1578
Size: 432 1146
Bpu10I CC'TnA_GC
Cuts at: 0 763 1141 1578
Size: 763 378 437
Fragments arranged by size:
763 437 378
BseYI C'CCAG_C
Cuts at: 0 101 176 1171 1499 1578
Size: 101 75 995 328 79
Fragments arranged by size:
995 328 101 79 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1578
Size: 782 796
BsiEI CG_ry'CG
Cuts at: 0 291 1578
Size: 291 1287
BsiHKAI G_wGCw'C
Cuts at: 0 851 1406 1578
Size: 851 555 172
Fragments arranged by size:
851 555 172
Bsp1286I G_dGCh'C
Cuts at: 0 8 278 851 1406 1578
Size: 8 270 573 555 172
Fragments arranged by size:
573 555 270 172 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 804 901 1048 1578
Size: 804 97 147 530
Fragments arranged by size:
804 530 147 97
BsrDI GCAATG_nn'
Cuts at: 0 1286 1578
Size: 1286 292
BsrFI r'CCGG_y
Cuts at: 0 1369 1578
Size: 1369 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1578
Size: 468 1110
BstYI r'GATC_y
Cuts at: 0 381 883 1578
Size: 381 502 695
Fragments arranged by size:
695 502 381
BstZ17I GTA'TAC
Cuts at: 0 420 1578
Size: 420 1158
BtsI GCAGTG_nn'
Cuts at: 0 95 681 1578
Size: 95 586 897
Fragments arranged by size:
897 586 95
DraIII CAC_nnn'GTG
Cuts at: 0 30 1578
Size: 30 1548
EcoICRI GAG'CTC
Cuts at: 0 849 1578
Size: 849 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 716 1505 1578
Size: 432 284 789 73
Fragments arranged by size:
789 432 284 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1107 1578
Size: 1107 471
EcoO109I rG'GnC_Cy
Cuts at: 0 274 593 631 1578
Size: 274 319 38 947
Fragments arranged by size:
947 319 274 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 560 585 617 1009 1041 1578
Size: 528 32 25 32 392 32 537
Fragments arranged by size:
537 528 392 32 32 32 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1445 1578
Size: 485 960 133
Fragments arranged by size:
960 485 133
MslI CAynn'nnrTG
Cuts at: 0 868 1442 1578
Size: 868 574 136
Fragments arranged by size:
868 574 136
MspA1I CmG'CkG
Cuts at: 0 197 287 576 1578
Size: 197 90 289 1002
Fragments arranged by size:
1002 289 197 90
NsiI A_TGCA'T
Cuts at: 0 315 1578
Size: 315 1263
NspI r_CATG'y
Cuts at: 0 1349 1578
Size: 1349 229
PasI CC'CwG_GG
Cuts at: 0 271 1125 1578
Size: 271 854 453
Fragments arranged by size:
854 453 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 1073 1578
Size: 1073 505
PpuMI rG'GwC_Cy
Cuts at: 0 593 631 1578
Size: 593 38 947
Fragments arranged by size:
947 593 38
PshAI GACnn'nnGTC
Cuts at: 0 934 1006 1578
Size: 934 72 572
Fragments arranged by size:
934 572 72
PspOMI G'GGCC_C
Cuts at: 0 274 1578
Size: 274 1304
PstI C_TGCA'G
Cuts at: 0 575 1578
Size: 575 1003
PvuII CAG'CTG
Cuts at: 0 197 576 1578
Size: 197 379 1002
Fragments arranged by size:
1002 379 197
SacI G_AGCT'C
Cuts at: 0 851 1578
Size: 851 727
ScaI AGT'ACT
Cuts at: 0 805 1578
Size: 805 773
SfcI C'TryA_G
Cuts at: 0 192 571 1578
Size: 192 379 1007
Fragments arranged by size:
1007 379 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1578
Size: 352 1226
TatI w'GTAC_w
Cuts at: 0 803 1578
Size: 803 775
Tth111I GACn'n_nGTC
Cuts at: 0 682 1578
Size: 682 896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 1578
Size: 184 1394
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI BamHI BanI BanII
BbsI BbvCI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI BstZ17I BtsI DraIII EcoICRI Eco57MI EcoNI
EcoO109I Hin4I MmeI MslI MspA1I NsiI NspI PasI
PflMI PpuMI PshAI PspOMI PstI PvuII SacI ScaI
SfcI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BciVI BclI BglI
BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI XbaI XhoI XmaI XmnI ZraI