(Linear) (Six-base) MAPSORT of: KIEE2536.seq  Check: 1111  from: 1  to: 369



KIEE2536.orf

 With 182 enzymes: SgfI * 

                        July 9, 2014 09:50  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     133     369 
   Size:        133     236
 
ApaI G_GGCC'C

Cuts at:      0     327     369 
   Size:        327      42
 
AvaI C'yCGr_G

Cuts at:      0      10     320     369 
   Size:         10     310      49

  Fragments arranged by size: 

                 310      49      10
 
BanI G'GyrC_C

Cuts at:      0     271     369 
   Size:        271      98
 
BanII G_rGCy'C

Cuts at:      0     327     369 
   Size:        327      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     180     369 
   Size:        180     189
 
Bme1580I G_kGCm'C

Cuts at:      0     274     327     369 
   Size:        274      53      42

  Fragments arranged by size: 

                 274      53      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     278     369 
   Size:        278      91
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     318     369 
   Size:        318      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     369 
   Size:        120     249
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     369 
   Size:        307      62
 
BseYI C'CCAG_C

Cuts at:      0      15      33      59     369 
   Size:         15      18      26     310

  Fragments arranged by size: 

                 310      26      18      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     337     369 
   Size:        337      32
 
Bsp1286I G_dGCh'C

Cuts at:      0     274     327     369 
   Size:        274      53      42

  Fragments arranged by size: 

                 274      53      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     161     369 
   Size:        161     208
 
BsrFI r'CCGG_y

Cuts at:      0     278     369 
   Size:        278      91
 
Bsu36I CC'TnA_GG

Cuts at:      0     329     369 
   Size:        329      40
 
BtsI GCAGTG_nn'

Cuts at:      0     170     369 
   Size:        170     199
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     369 
   Size:        120     249
 
EcoO109I rG'GnC_Cy

Cuts at:      0     324     369 
   Size:        324      45
 
HaeII r_GCGC'y

Cuts at:      0      34     369 
   Size:         34     335
 
HindIII A'AGCT_T

Cuts at:      0     244     369 
   Size:        244     125
 
MspA1I CmG'CkG

Cuts at:      0      59     369 
   Size:         59     310
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     369 
   Size:        283      32      54

  Fragments arranged by size: 

                 283      54      32
 
PspOMI G'GGCC_C

Cuts at:      0     323     369 
   Size:        323      46
 
SmaI CCC'GGG

Cuts at:      0      12     322     369 
   Size:         12     310      47

  Fragments arranged by size: 

                 310      47      12
 
SrfI GCCC'GGGC

Cuts at:      0     322     369 
   Size:        322      47
 
StyI C'CwwG_G

Cuts at:      0     338     369 
   Size:        338      31
 
XmaI C'CCGG_G

Cuts at:      0      10     320     369 
   Size:         10     310      49

  Fragments arranged by size: 

                 310      49      10
 

 Enzymes that do cut:

     AlfI     ApaI     AvaI     BanI    BanII     BbsI Bme1580I     BmrI
     BplI     BpmI    BseRI    BseYI    BsmBI Bsp1286I    BspMI    BsrFI
   Bsu36I     BtsI  Eco57MI EcoO109I    HaeII  HindIII   MspA1I     PpiI
   PspOMI     SmaI     SrfI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI