Restriction map for
pF1KE2538
(Linear) (Six-base) MAPSORT of: KIEE2538.seq Check: 3539 from: 1 to: 7692
KIEE2538.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 6162 7692
Size: 594 5568 1530
Fragments arranged by size:
5568 1530 594
AccI GT'mk_AC
Cuts at: 0 329 548 3094 3684 3933 7692
Size: 329 219 2546 590 249 3759
Fragments arranged by size:
3759 2546 590 329 249 219
AclI AA'CG_TT
Cuts at: 0 4987 7692
Size: 4987 2705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 213 307 962 1547 2426 2441 2632
Size: 89 124 94 655 585 879 15 191
Cuts at: 2632 4063 4246 4344 4846 4955 5320 5371 6123
Size: 1431 183 98 502 109 365 51 752
Cuts at: 6123 6967 7526 7582 7692
Size: 844 559 56 110
Fragments arranged by size:
1431 879 844 752 655 585 559 502
365 191 183 124 110 109 98 94
89 56 51 15
AflIII A'CryG_T
Cuts at: 0 795 2964 3732 3886 6681 6901 7692
Size: 795 2169 768 154 2795 220 791
Fragments arranged by size:
2795 2169 795 791 768 220 154
AhdI GACnn_n'nnGTC
Cuts at: 0 4092 7692
Size: 4092 3600
AleI CACnn'nnGTG
Cuts at: 0 4889 7692
Size: 4889 2803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2073 7692
Size: 2073 5619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1598 1630 7692
Size: 1598 32 6062
Fragments arranged by size:
6062 1598 32
AlwNI CAG_nnn'CTG
Cuts at: 0 967 2041 2587 5602 5866 6429 6694 7455
Size: 967 1074 546 3015 264 563 265 761
Cuts at: 7455 7692
Size: 237
Fragments arranged by size:
3015 1074 967 761 563 546 265 264
237
ApaI G_GGCC'C
Cuts at: 0 4340 7692
Size: 4340 3352
ApoI r'AATT_y
Cuts at: 0 422 872 1018 1702 1710 1915 2517 2536
Size: 422 450 146 684 8 205 602 19
Cuts at: 2536 2898 3015 3222 3346 3425 3552 4063 6071
Size: 362 117 207 124 79 127 511 2008
Cuts at: 6071 7692
Size: 1621
Fragments arranged by size:
2008 1621 684 602 511 450 422 362
207 205 146 127 124 117 79 19
8
AseI AT'TA_AT
Cuts at: 0 1808 5921 7692
Size: 1808 4113 1771
Fragments arranged by size:
4113 1808 1771
AvaI C'yCGr_G
Cuts at: 0 4772 7193 7692
Size: 4772 2421 499
Fragments arranged by size:
4772 2421 499
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2330 2363 3838 3871 7692
Size: 2330 33 1475 33 3821
Fragments arranged by size:
3821 2330 1475 33 33
BamHI G'GATC_C
Cuts at: 0 3918 4036 6531 7251 7692
Size: 3918 118 2495 720 441
Fragments arranged by size:
3918 2495 720 441 118
BanI G'GyrC_C
Cuts at: 0 172 6675 7692
Size: 172 6503 1017
Fragments arranged by size:
6503 1017 172
BanII G_rGCy'C
Cuts at: 0 1063 1312 3764 4340 4530 5885 7692
Size: 1063 249 2452 576 190 1355 1807
Fragments arranged by size:
2452 1807 1355 1063 576 249 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 1405 2647 2931 3035 4561 6169 6973 7192
Size: 1405 1242 284 104 1526 1608 804 219
Cuts at: 7192 7514 7692
Size: 322 178
Fragments arranged by size:
1608 1526 1405 1242 804 322 284 219
178 104
BbvCI CC'TCA_GC
Cuts at: 0 7082 7692
Size: 7082 610
BclI T'GATC_A
Cuts at: 0 1980 5727 6609 7229 7692
Size: 1980 3747 882 620 463
Fragments arranged by size:
3747 1980 882 620 463
BfrBI ATG'CAT
Cuts at: 0 2141 3052 7692
Size: 2141 911 4640
Fragments arranged by size:
4640 2141 911
BglII A'GATC_T
Cuts at: 0 706 1210 7692
Size: 706 504 6482
Fragments arranged by size:
6482 706 504
BlpI GC'TnA_GC
Cuts at: 0 1062 5206 5590 6617 7692
Size: 1062 4144 384 1027 1075
Fragments arranged by size:
4144 1075 1062 1027 384
Bme1580I G_kGCm'C
Cuts at: 0 4316 4340 7692
Size: 4316 24 3352
Fragments arranged by size:
4316 3352 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1618 3697 3924 3956 6580 6708 6784 7431
Size: 1618 2079 227 32 2624 128 76 647
Cuts at: 7431 7692
Size: 261
Fragments arranged by size:
2624 2079 1618 647 261 227 128 76
32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1229 1251 4765 5202 7692
Size: 1229 22 3514 437 2490
Fragments arranged by size:
3514 2490 1229 437 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1826 2699 3181 6738 7371 7609 7692
Size: 293 1533 873 482 3557 633 238 83
Fragments arranged by size:
3557 1533 873 633 482 293 238 83
Bpu10I CC'TnA_GC
Cuts at: 0 7082 7692
Size: 7082 610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1392 2870 3773 3953 4514 4710 6857 7312
Size: 1392 1478 903 180 561 196 2147 455
Cuts at: 7312 7692
Size: 380
Fragments arranged by size:
2147 1478 1392 903 561 455 380 196
180
BsaI GGTCTCn'nnnn_
Cuts at: 0 1436 1486 1844 5188 5486 6182 7692
Size: 1436 50 358 3344 298 696 1510
Fragments arranged by size:
3344 1510 1436 696 358 298 50
BsaAI yAC'GTr
Cuts at: 0 798 3809 3889 7692
Size: 798 3011 80 3803
Fragments arranged by size:
3803 3011 798 80
BsaBI GATnn'nnATC
Cuts at: 0 1182 2609 5726 7692
Size: 1182 1427 3117 1966
Fragments arranged by size:
3117 1966 1427 1182
BsaWI w'CCGG_w
Cuts at: 0 3420 7466 7692
Size: 3420 4046 226
Fragments arranged by size:
4046 3420 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2616 2646 2951 2981 5598 5628 5768 5798
Size: 2616 30 305 30 2617 30 140 30
Cuts at: 5798 5944 5974 6736 6766 7019 7049 7549 7579
Size: 146 30 762 30 253 30 500 30
Cuts at: 7579 7692
Size: 113
Fragments arranged by size:
2617 2616 762 500 305 253 146 140
113 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2966 4027 5084 5965 7136 7692
Size: 2966 1061 1057 881 1171 556
Fragments arranged by size:
2966 1171 1061 1057 881 556
BseYI C'CCAG_C
Cuts at: 0 1834 4682 6535 6574 6971 7692
Size: 1834 2848 1853 39 397 721
Fragments arranged by size:
2848 1853 1834 721 397 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 254 6848 7692
Size: 124 130 6594 844
Fragments arranged by size:
6594 844 130 124
BsiEI CG_ry'CG
Cuts at: 0 5367 7692
Size: 5367 2325
BsiHKAI G_wGCw'C
Cuts at: 0 59 1063 1312 1365 3764 4530 5207 5553
Size: 59 1004 249 53 2399 766 677 346
Cuts at: 5553 5596 6563 7692
Size: 43 967 1129
Fragments arranged by size:
2399 1129 1004 967 766 677 346 249
59 53 43
BsmI GAATG_Cn'
Cuts at: 0 4493 5809 7692
Size: 4493 1316 1883
Fragments arranged by size:
4493 1883 1316
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3199 7692
Size: 3199 4493
Bsp1286I G_dGCh'C
Cuts at: 0 59 1063 1312 1365 3764 4316 4340 4530
Size: 59 1004 249 53 2399 552 24 190
Cuts at: 4530 5207 5553 5596 5885 6563 7692
Size: 677 346 43 289 678 1129
Fragments arranged by size:
2399 1129 1004 678 677 552 346 289
249 190 59 53 43 24
BspEI T'CCGG_A
Cuts at: 0 3420 7692
Size: 3420 4272
BspHI T'CATG_A
Cuts at: 0 3102 4574 4877 7692
Size: 3102 1472 303 2815
Fragments arranged by size:
3102 2815 1472 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 2212 6162 7692
Size: 594 1618 3950 1530
Fragments arranged by size:
3950 1618 1530 594
BsrBI CCG'CTC
Cuts at: 0 6267 7692
Size: 6267 1425
BsrDI GCAATG_nn'
Cuts at: 0 285 3912 7602 7692
Size: 285 3627 3690 90
Fragments arranged by size:
3690 3627 285 90
BsrFI r'CCGG_y
Cuts at: 0 6777 7692
Size: 6777 915
BsrGI T'GTAC_A
Cuts at: 0 1723 4501 6843 6957 7692
Size: 1723 2778 2342 114 735
Fragments arranged by size:
2778 2342 1723 735 114
BssSI C'ACGA_G
Cuts at: 0 1364 7692
Size: 1364 6328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 6420 7692
Size: 6420 1272
BstBI TT'CG_AA
Cuts at: 0 4714 7692
Size: 4714 2978
BstEII G'GTnAC_C
Cuts at: 0 3544 5059 6496 7692
Size: 3544 1515 1437 1196
Fragments arranged by size:
3544 1515 1437 1196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4376 4962 5011 6036 6733 7692
Size: 4376 586 49 1025 697 959
Fragments arranged by size:
4376 1025 959 697 586 49
BstYI r'GATC_y
Cuts at: 0 706 1083 1210 2023 3918 4036 5339 5721
Size: 706 377 127 813 1895 118 1303 382
Cuts at: 5721 6531 7128 7197 7251 7692
Size: 810 597 69 54 441
Fragments arranged by size:
1895 1303 813 810 706 597 441 382
377 127 118 69 54
Bsu36I CC'TnA_GG
Cuts at: 0 1447 7580 7692
Size: 1447 6133 112
Fragments arranged by size:
6133 1447 112
BtgI C'CryG_G
Cuts at: 0 3023 3236 7692
Size: 3023 213 4456
Fragments arranged by size:
4456 3023 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4389 5528 7692
Size: 4389 1139 2164
Fragments arranged by size:
4389 2164 1139
BtsI GCAGTG_nn'
Cuts at: 0 266 463 530 2735 4498 6445 7058 7692
Size: 266 197 67 2205 1763 1947 613 634
Fragments arranged by size:
2205 1947 1763 634 613 266 197 67
ClaI AT'CG_AT
Cuts at: 0 1857 7692
Size: 1857 5835
DraI TTT'AAA
Cuts at: 0 1016 1053 1073 1707 1880 2762 4767 7515
Size: 1016 37 20 634 173 882 2005 2748
Cuts at: 7515 7692
Size: 177
Fragments arranged by size:
2748 2005 1016 882 634 177 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 0 6902 7692
Size: 6902 790
EaeI y'GGCC_r
Cuts at: 0 1342 5364 6023 7692
Size: 1342 4022 659 1669
Fragments arranged by size:
4022 1669 1342 659
EagI C'GGCC_G
Cuts at: 0 5364 7692
Size: 5364 2328
EarI CTCTTCn'nnn_
Cuts at: 0 326 1382 1437 1512 2651 2913 3126 3593
Size: 326 1056 55 75 1139 262 213 467
Cuts at: 3593 3752 4004 5387 5750 5942 6258 7692
Size: 159 252 1383 363 192 316 1434
Fragments arranged by size:
1434 1383 1139 1056 467 363 326 316
262 252 213 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 557 1218 7692
Size: 557 661 6474
Fragments arranged by size:
6474 661 557
EcoICRI GAG'CTC
Cuts at: 0 1061 1310 3762 4528 7692
Size: 1061 249 2452 766 3164
Fragments arranged by size:
3164 2452 1061 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 213 293 307 962 1547 1826 2426
Size: 89 124 80 14 655 585 279 600
Cuts at: 2426 2441 2632 2699 3181 4063 4246 4344 4846
Size: 15 191 67 482 882 183 98 502
Cuts at: 4846 4955 5320 5371 6123 6738 6967 7371 7526
Size: 109 365 51 752 615 229 404 155
Cuts at: 7526 7582 7609 7692
Size: 56 27 83
Fragments arranged by size:
882 752 655 615 600 585 502 482
404 365 279 229 191 183 155 124
109 98 89 83 80 67 56 51
27 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1257 2813 6058 7692
Size: 1257 1556 3245 1634
Fragments arranged by size:
3245 1634 1556 1257
EcoO109I rG'GnC_Cy
Cuts at: 0 4204 4337 4365 7584 7692
Size: 4204 133 28 3219 108
Fragments arranged by size:
4204 3219 133 108 28
EcoRI G'AATT_C
Cuts at: 0 2898 3222 6071 7692
Size: 2898 324 2849 1621
Fragments arranged by size:
2898 2849 1621 324
EcoRV GAT'ATC
Cuts at: 0 4191 7692
Size: 4191 3501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4576 5740 7692
Size: 4576 1164 1952
Fragments arranged by size:
4576 1952 1164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 760 792 817 849 1948 1980
Size: 355 32 373 32 25 32 1099 32
Cuts at: 1980 2695 2727 2918 2950 3514 3546 3910 3942
Size: 715 32 191 32 564 32 364 32
Cuts at: 3942 4078 4110 5072 5104 5527 5559 5944 5976
Size: 136 32 962 32 423 32 385 32
Cuts at: 5976 7104 7136 7692
Size: 1128 32 556
Fragments arranged by size:
1128 1099 962 715 564 556 423 385
373 364 355 191 136 32 32 32
32 32 32 32 32 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 4985 7692
Size: 4985 2707
HindIII A'AGCT_T
Cuts at: 0 5383 7692
Size: 5383 2309
HpaI GTT'AAC
Cuts at: 0 4985 7692
Size: 4985 2707
MfeI C'AATT_G
Cuts at: 0 5049 6953 7692
Size: 5049 1904 739
Fragments arranged by size:
5049 1904 739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1641 5423 6025 6931 7348 7692
Size: 1641 3782 602 906 417 344
Fragments arranged by size:
3782 1641 906 602 417 344
MscI TGG'CCA
Cuts at: 0 6025 7692
Size: 6025 1667
MslI CAynn'nnrTG
Cuts at: 0 1280 2284 2600 3057 3335 3521 3737 4374
Size: 1280 1004 316 457 278 186 216 637
Cuts at: 4374 4649 4889 7692
Size: 275 240 2803
Fragments arranged by size:
2803 1280 1004 637 457 316 278 275
240 216 186
MspA1I CmG'CkG
Cuts at: 0 9 313 4889 5796 7692
Size: 9 304 4576 907 1896
Fragments arranged by size:
4576 1896 907 304 9
NaeI GCC'GGC
Cuts at: 0 6779 7692
Size: 6779 913
NcoI C'CATG_G
Cuts at: 0 3023 3236 7692
Size: 3023 213 4456
Fragments arranged by size:
4456 3023 213
NdeI CA'TA_TG
Cuts at: 0 7329 7692
Size: 7329 363
NgoMIV G'CCGG_C
Cuts at: 0 6777 7692
Size: 6777 915
NsiI A_TGCA'T
Cuts at: 0 2143 3054 7692
Size: 2143 911 4638
Fragments arranged by size:
4638 2143 911
NspI r_CATG'y
Cuts at: 0 2141 2145 2211 2546 2770 2968 3736 4547
Size: 2141 4 66 335 224 198 768 811
Cuts at: 4547 4648 6685 6905 7644 7692
Size: 101 2037 220 739 48
Fragments arranged by size:
2141 2037 811 768 739 335 224 220
198 101 66 48 4
PciI A'CATG_T
Cuts at: 0 2964 3732 6681 6901 7692
Size: 2964 768 2949 220 791
Fragments arranged by size:
2964 2949 791 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 6720 7692
Size: 6720 972
PfoI T'CCnGG_A
Cuts at: 0 3939 6035 7692
Size: 3939 2096 1657
Fragments arranged by size:
3939 2096 1657
PmlI CAC'GTG
Cuts at: 0 3889 7692
Size: 3889 3803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5269 5301 7692
Size: 5269 32 2391
Fragments arranged by size:
5269 2391 32
PpuMI rG'GwC_Cy
Cuts at: 0 4365 7584 7692
Size: 4365 3219 108
Fragments arranged by size:
4365 3219 108
PshAI GACnn'nnGTC
Cuts at: 0 1623 5497 7692
Size: 1623 3874 2195
Fragments arranged by size:
3874 2195 1623
PsiI TTA'TAA
Cuts at: 0 730 1664 1921 5401 5650 6470 7692
Size: 730 934 257 3480 249 820 1222
Fragments arranged by size:
3480 1222 934 820 730 257 249
PspOMI G'GGCC_C
Cuts at: 0 4336 7692
Size: 4336 3356
PspXI vC'TCGA_Gb
Cuts at: 0 4772 7692
Size: 4772 2920
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2562 2594 2733 2765 7692
Size: 2562 32 139 32 4927
Fragments arranged by size:
4927 2562 139 32 32
PstI C_TGCA'G
Cuts at: 0 14 381 7692
Size: 14 367 7311
Fragments arranged by size:
7311 367 14
PvuII CAG'CTG
Cuts at: 0 9 313 4889 5796 7692
Size: 9 304 4576 907 1896
Fragments arranged by size:
4576 1896 907 304 9
SacI G_AGCT'C
Cuts at: 0 1063 1312 3764 4530 7692
Size: 1063 249 2452 766 3162
Fragments arranged by size:
3162 2452 1063 766 249
SanDI GG'GwC_CC
Cuts at: 0 4365 7692
Size: 4365 3327
SapI GCTCTTCn'nnn_
Cuts at: 0 3126 3593 3752 7692
Size: 3126 467 159 3940
Fragments arranged by size:
3940 3126 467 159
ScaI AGT'ACT
Cuts at: 0 5459 6856 7567 7692
Size: 5459 1397 711 125
Fragments arranged by size:
5459 1397 711 125
SexAI A'CCwGG_T
Cuts at: 0 5198 6504 7692
Size: 5198 1306 1188
Fragments arranged by size:
5198 1306 1188
SfcI C'TryA_G
Cuts at: 0 10 377 545 3095 5747 6350 6638 7692
Size: 10 367 168 2550 2652 603 288 1054
Fragments arranged by size:
2652 2550 1054 603 367 288 168 10
SmlI C'TyrA_G
Cuts at: 0 1407 2885 3788 3968 4529 4689 4772 6872
Size: 1407 1478 903 180 561 160 83 2100
Cuts at: 6872 7193 7291 7692
Size: 321 98 401
Fragments arranged by size:
2100 1478 1407 903 561 401 321 180
160 98 83
SpeI A'CTAG_T
Cuts at: 0 2575 7692
Size: 2575 5117
SphI G_CATG'C
Cuts at: 0 2141 2145 2211 2770 4648 7692
Size: 2141 4 66 559 1878 3044
Fragments arranged by size:
3044 2141 1878 559 66 4
SspI AAT'ATT
Cuts at: 0 809 1129 2169 2433 4735 7692
Size: 809 320 1040 264 2302 2957
Fragments arranged by size:
2957 2302 1040 809 320 264
StuI AGG'CCT
Cuts at: 0 6523 7351 7692
Size: 6523 828 341
Fragments arranged by size:
6523 828 341
StyI C'CwwG_G
Cuts at: 0 3023 3236 6623 7619 7692
Size: 3023 213 3387 996 73
Fragments arranged by size:
3387 3023 996 213 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3563 6588 7692
Size: 3563 3025 1104
Fragments arranged by size:
3563 3025 1104
TatI w'GTAC_w
Cuts at: 0 1723 2383 3508 4501 5457 6741 6843 6854
Size: 1723 660 1125 993 956 1284 102 11
Cuts at: 6854 6957 7565 7625 7637 7692
Size: 103 608 60 12 55
Fragments arranged by size:
1723 1284 1125 993 956 660 608 103
102 60 55 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2657 2689 2951 2983 4371 4403 5275 5307
Size: 2657 32 262 32 1388 32 872 32
Cuts at: 5307 5559 5591 5598 5630 6574 6606 6736 6768
Size: 252 32 7 32 944 32 130 32
Cuts at: 6768 7002 7034 7576 7608 7692
Size: 234 32 542 32 84
Fragments arranged by size:
2657 1388 944 872 542 262 252 234
130 84 32 32 32 32 32 32
32 32 32 32 7
XbaI T'CTAG_A
Cuts at: 0 1237 7692
Size: 1237 6455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 3644 6033 6576 7692
Size: 465 3179 2389 543 1116
Fragments arranged by size:
3179 2389 1116 543 465
XhoI C'TCGA_G
Cuts at: 0 4772 7193 7692
Size: 4772 2421 499
Fragments arranged by size:
4772 2421 499
XmnI GAAnn'nnTTC
Cuts at: 0 872 5032 7692
Size: 872 4160 2660
Fragments arranged by size:
4160 2660 872
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII HpaI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII SacI SanDI
SapI ScaI SexAI SfcI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI ApaLI AscI AsiSI
AvrII BbeI BcgI BciVI BglI BmgBI BmtI BsaHI
BsiWI BssHII BstZ17I CspCI DraIII FseI FspI FspAI
HaeII KasI KpnI MluI NarI NheI NotI NruI
PacI PasI PmeI PvuI RsrII SacII SalI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI
Tth111I XmaI ZraI