Restriction map for
pF1KE2548
(Linear) (Six-base) MAPSORT of: KIEE2548.seq Check: 7648 from: 1 to: 2820
KIEE2548.orf
With 182 enzymes: SgfI *
October 24, 2014 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1135 2820
Size: 1135 1685
AatII G_ACGT'C
Cuts at: 0 1248 2820
Size: 1248 1572
AccI GT'mk_AC
Cuts at: 0 1634 2024 2820
Size: 1634 390 796
Fragments arranged by size:
1634 796 390
AclI AA'CG_TT
Cuts at: 0 585 2820
Size: 585 2235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 991 1966 2092 2602 2767 2820
Size: 355 636 975 126 510 165 53
Fragments arranged by size:
975 636 510 355 165 126 53
AflIII A'CryG_T
Cuts at: 0 2063 2820
Size: 2063 757
AlwNI CAG_nnn'CTG
Cuts at: 0 1665 2745 2820
Size: 1665 1080 75
Fragments arranged by size:
1665 1080 75
ApaI G_GGCC'C
Cuts at: 0 1213 2600 2759 2820
Size: 1213 1387 159 61
Fragments arranged by size:
1387 1213 159 61
ApaLI G'TGCA_C
Cuts at: 0 1642 2041 2820
Size: 1642 399 779
Fragments arranged by size:
1642 779 399
ApoI r'AATT_y
Cuts at: 0 2446 2820
Size: 2446 374
AvaI C'yCGr_G
Cuts at: 0 737 875 1981 2820
Size: 737 138 1106 839
Fragments arranged by size:
1106 839 737 138
BamHI G'GATC_C
Cuts at: 0 1023 2820
Size: 1023 1797
BanI G'GyrC_C
Cuts at: 0 1123 2250 2820
Size: 1123 1127 570
Fragments arranged by size:
1127 1123 570
BanII G_rGCy'C
Cuts at: 0 924 1213 1340 1837 2165 2532 2600 2759
Size: 924 289 127 497 328 367 68 159
Cuts at: 2759 2820
Size: 61
Fragments arranged by size:
924 497 367 328 289 159 127 68
61
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 526 1868 2770 2820
Size: 365 161 1342 902 50
Fragments arranged by size:
1342 902 365 161 50
BbvCI CC'TCA_GC
Cuts at: 0 277 2820
Size: 277 2543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1972 2006 2820
Size: 1972 34 814
Fragments arranged by size:
1972 814 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2422 2687 2820
Size: 2422 265 133
Fragments arranged by size:
2422 265 133
BglI GCCn_nnn'nGGC
Cuts at: 0 2198 2820
Size: 2198 622
BglII A'GATC_T
Cuts at: 0 681 2820
Size: 681 2139
BlpI GC'TnA_GC
Cuts at: 0 2159 2820
Size: 2159 661
Bme1580I G_kGCm'C
Cuts at: 0 1126 1213 1646 2045 2255 2600 2759 2820
Size: 1126 87 433 399 210 345 159 61
Fragments arranged by size:
1126 433 399 345 210 159 87 61
BmgBI CAC'GTC
Cuts at: 0 438 2457 2820
Size: 438 2019 363
Fragments arranged by size:
2019 438 363
BmrI ACTGGGnnnn_n'
Cuts at: 0 1753 2224 2820
Size: 1753 471 596
Fragments arranged by size:
1753 596 471
BmtI G_CTAG'C
Cuts at: 0 643 2820
Size: 643 2177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2659 2820
Size: 2659 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1176 1260 1326 1434 2013 2820
Size: 571 605 84 66 108 579 807
Fragments arranged by size:
807 605 579 571 108 84 66
Bpu10I CC'TnA_GC
Cuts at: 0 277 1118 2820
Size: 277 841 1702
Fragments arranged by size:
1702 841 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 301 1488 1839 2660 2820
Size: 238 63 1187 351 821 160
Fragments arranged by size:
1187 821 351 238 160 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 1807 1815 2820
Size: 1807 8 1005
Fragments arranged by size:
1807 1005 8
BsaAI yAC'GTr
Cuts at: 0 2066 2820
Size: 2066 754
BsaHI Gr'CG_yC
Cuts at: 0 1245 2820
Size: 1245 1575
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 899 929 1458 1488 1606 1636 2006 2036
Size: 899 30 529 30 118 30 370 30
Cuts at: 2036 2820
Size: 784
Fragments arranged by size:
899 784 529 370 118 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 106 109 112 1026 1179 1309 1406
Size: 103 3 3 3 914 153 130 97
Cuts at: 1406 2230 2674 2820
Size: 824 444 146
Fragments arranged by size:
914 824 444 153 146 130 103 97
3 3 3
BseYI C'CCAG_C
Cuts at: 0 429 711 1238 1432 1727 2165 2290 2820
Size: 429 282 527 194 295 438 125 530
Fragments arranged by size:
530 527 438 429 295 282 194 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1560 2820
Size: 1560 1260
BsiEI CG_ry'CG
Cuts at: 0 20 2820
Size: 20 2800
BsiHKAI G_wGCw'C
Cuts at: 0 1340 1646 2045 2142 2462 2820
Size: 1340 306 399 97 320 358
Fragments arranged by size:
1340 399 358 320 306 97
BsmI GAATG_Cn'
Cuts at: 0 2052 2820
Size: 2052 768
Bsp1286I G_dGCh'C
Cuts at: 0 924 1126 1213 1340 1646 1837 2045 2142
Size: 924 202 87 127 306 191 208 97
Cuts at: 2142 2165 2255 2462 2532 2600 2759 2820
Size: 23 90 207 70 68 159 61
Fragments arranged by size:
924 306 208 207 202 191 159 127
97 90 87 70 68 61 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 842 1135 2333 2820
Size: 842 293 1198 487
Fragments arranged by size:
1198 842 487 293
BsrBI CCG'CTC
Cuts at: 0 1263 1414 2820
Size: 1263 151 1406
Fragments arranged by size:
1406 1263 151
BsrDI GCAATG_nn'
Cuts at: 0 372 2820
Size: 372 2448
BsrFI r'CCGG_y
Cuts at: 0 19 2820
Size: 19 2801
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2049 2820
Size: 2049 771
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2697 2820
Size: 2697 123
BstYI r'GATC_y
Cuts at: 0 681 1023 2820
Size: 681 342 1797
Fragments arranged by size:
1797 681 342
Bsu36I CC'TnA_GG
Cuts at: 0 295 2820
Size: 295 2525
BtgI C'CryG_G
Cuts at: 0 31 2237 2397 2820
Size: 31 2206 160 423
Fragments arranged by size:
2206 423 160 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 296 592 1559 2820
Size: 296 296 967 1261
Fragments arranged by size:
1261 967 296 296
BtsI GCAGTG_nn'
Cuts at: 0 1904 2820
Size: 1904 916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1893 1928 2820
Size: 1893 35 892
Fragments arranged by size:
1893 892 35
DraIII CAC_nnn'GTG
Cuts at: 0 1650 2343 2820
Size: 1650 693 477
Fragments arranged by size:
1650 693 477
DrdI GACnn_nn'nnGTC
Cuts at: 0 1729 2820
Size: 1729 1091
EaeI y'GGCC_r
Cuts at: 0 17 224 1254 2234 2552 2687 2820
Size: 17 207 1030 980 318 135 133
Fragments arranged by size:
1030 980 318 207 135 133 17
EagI C'GGCC_G
Cuts at: 0 17 2820
Size: 17 2803
EarI CTCTTCn'nnn_
Cuts at: 0 127 331 368 395 473 1144 1515 2287
Size: 127 204 37 27 78 671 371 772
Cuts at: 2287 2651 2652 2820
Size: 364 1 168
Fragments arranged by size:
772 671 371 364 204 168 127 78
37 27 1
EcoICRI GAG'CTC
Cuts at: 0 1338 2820
Size: 1338 1482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 571 991 1176 1260 1326 1434 1966
Size: 355 216 420 185 84 66 108 532
Cuts at: 1966 2013 2092 2602 2767 2820
Size: 47 79 510 165 53
Fragments arranged by size:
532 510 420 355 216 185 165 108
84 79 66 53 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 2820
Size: 319 2501
EcoO109I rG'GnC_Cy
Cuts at: 0 1209 1454 1479 2820
Size: 1209 245 25 1341
Fragments arranged by size:
1341 1209 245 25
EcoRI G'AATT_C
Cuts at: 0 2446 2820
Size: 2446 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2170 2820
Size: 2170 650
FspI TGC'GCA
Cuts at: 0 49 2820
Size: 49 2771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 2006 2038 2420 2452 2658 2690
Size: 1008 32 966 32 382 32 206 32
Cuts at: 2690 2820
Size: 130
Fragments arranged by size:
1008 966 382 206 130 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1485 2820
Size: 1485 1335
MscI TGG'CCA
Cuts at: 0 226 2554 2820
Size: 226 2328 266
Fragments arranged by size:
2328 266 226
MslI CAynn'nnrTG
Cuts at: 0 2627 2820
Size: 2627 193
MspA1I CmG'CkG
Cuts at: 0 33 1322 1495 1990 2169 2319 2820
Size: 33 1289 173 495 179 150 501
Fragments arranged by size:
1289 501 495 179 173 150 33
NaeI GCC'GGC
Cuts at: 0 21 2820
Size: 21 2799
NcoI C'CATG_G
Cuts at: 0 2397 2820
Size: 2397 423
NgoMIV G'CCGG_C
Cuts at: 0 19 2820
Size: 19 2801
NheI G'CTAG_C
Cuts at: 0 639 2820
Size: 639 2181
NspI r_CATG'y
Cuts at: 0 691 2820
Size: 691 2129
PfoI T'CCnGG_A
Cuts at: 0 1026 1450 2820
Size: 1026 424 1370
Fragments arranged by size:
1370 1026 424
PmlI CAC'GTG
Cuts at: 0 2066 2820
Size: 2066 754
PpuMI rG'GwC_Cy
Cuts at: 0 1454 1479 2820
Size: 1454 25 1341
Fragments arranged by size:
1454 1341 25
PsiI TTA'TAA
Cuts at: 0 262 1289 2820
Size: 262 1027 1531
Fragments arranged by size:
1531 1027 262
PspOMI G'GGCC_C
Cuts at: 0 1209 2596 2755 2820
Size: 1209 1387 159 65
Fragments arranged by size:
1387 1209 159 65
PstI C_TGCA'G
Cuts at: 0 181 673 839 929 1132 2820
Size: 181 492 166 90 203 1688
Fragments arranged by size:
1688 492 203 181 166 90
PvuII CAG'CTG
Cuts at: 0 1990 2169 2319 2820
Size: 1990 179 150 501
Fragments arranged by size:
1990 501 179 150
SacI G_AGCT'C
Cuts at: 0 1340 2820
Size: 1340 1480
SacII CC_GC'GG
Cuts at: 0 34 2820
Size: 34 2786
SapI GCTCTTCn'nnn_
Cuts at: 0 127 1515 2820
Size: 127 1388 1305
Fragments arranged by size:
1388 1305 127
SbfI CC_TGCA'GG
Cuts at: 0 1132 2820
Size: 1132 1688
SexAI A'CCwGG_T
Cuts at: 0 767 2820
Size: 767 2053
SfcI C'TryA_G
Cuts at: 0 177 669 835 925 955 1128 2820
Size: 177 492 166 90 30 173 1692
Fragments arranged by size:
1692 492 177 173 166 90 30
SmlI C'TyrA_G
Cuts at: 0 253 316 737 875 1503 1818 2675 2820
Size: 253 63 421 138 628 315 857 145
Fragments arranged by size:
857 628 421 315 253 145 138 63
SphI G_CATG'C
Cuts at: 0 691 2820
Size: 691 2129
StyI C'CwwG_G
Cuts at: 0 62 227 1004 1998 2397 2820
Size: 62 165 777 994 399 423
Fragments arranged by size:
994 777 423 399 165 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 2820
Size: 421 2399
TatI w'GTAC_w
Cuts at: 0 448 2820
Size: 448 2372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1446 1478 1606 1638 2820
Size: 1446 32 128 32 1182
Fragments arranged by size:
1446 1182 128 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1759 2368 2820
Size: 1759 609 452
Fragments arranged by size:
1759 609 452
XbaI T'CTAG_A
Cuts at: 0 1059 2820
Size: 1059 1761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1701 2380 2820
Size: 1701 679 440
Fragments arranged by size:
1701 679 440
XhoI C'TCGA_G
Cuts at: 0 737 875 2820
Size: 737 138 1945
Fragments arranged by size:
1945 737 138
XmnI GAAnn'nnTTC
Cuts at: 0 586 778 2820
Size: 586 192 2042
Fragments arranged by size:
2042 586 192
ZraI GAC'GTC
Cuts at: 0 1246 2820
Size: 1246 1574
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
Hin4I HindIII MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuII SacI SacII SapI SbfI SexAI SfcI SmlI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbeI BclI BfrBI
BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstBI BstEII BstZ17I ClaI DraI EciI EcoRV
FseI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PshAI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
XmaI