(Linear) (Six-base) MAPSORT of: KIEE2548.seq Check: 7648 from: 1 to: 2820 KIEE2548.orf With 182 enzymes: SgfI * October 24, 2014 10:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1135 2820 Size: 1135 1685 AatII G_ACGT'C Cuts at: 0 1248 2820 Size: 1248 1572 AccI GT'mk_AC Cuts at: 0 1634 2024 2820 Size: 1634 390 796 Fragments arranged by size: 1634 796 390 AclI AA'CG_TT Cuts at: 0 585 2820 Size: 585 2235 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 991 1966 2092 2602 2767 2820 Size: 355 636 975 126 510 165 53 Fragments arranged by size: 975 636 510 355 165 126 53 AflIII A'CryG_T Cuts at: 0 2063 2820 Size: 2063 757 AlwNI CAG_nnn'CTG Cuts at: 0 1665 2745 2820 Size: 1665 1080 75 Fragments arranged by size: 1665 1080 75 ApaI G_GGCC'C Cuts at: 0 1213 2600 2759 2820 Size: 1213 1387 159 61 Fragments arranged by size: 1387 1213 159 61 ApaLI G'TGCA_C Cuts at: 0 1642 2041 2820 Size: 1642 399 779 Fragments arranged by size: 1642 779 399 ApoI r'AATT_y Cuts at: 0 2446 2820 Size: 2446 374 AvaI C'yCGr_G Cuts at: 0 737 875 1981 2820 Size: 737 138 1106 839 Fragments arranged by size: 1106 839 737 138 BamHI G'GATC_C Cuts at: 0 1023 2820 Size: 1023 1797 BanI G'GyrC_C Cuts at: 0 1123 2250 2820 Size: 1123 1127 570 Fragments arranged by size: 1127 1123 570 BanII G_rGCy'C Cuts at: 0 924 1213 1340 1837 2165 2532 2600 2759 Size: 924 289 127 497 328 367 68 159 Cuts at: 2759 2820 Size: 61 Fragments arranged by size: 924 497 367 328 289 159 127 68 61 BbsI GAAGACnn'nnnn_ Cuts at: 0 365 526 1868 2770 2820 Size: 365 161 1342 902 50 Fragments arranged by size: 1342 902 365 161 50 BbvCI CC'TCA_GC Cuts at: 0 277 2820 Size: 277 2543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1972 2006 2820 Size: 1972 34 814 Fragments arranged by size: 1972 814 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2422 2687 2820 Size: 2422 265 133 Fragments arranged by size: 2422 265 133 BglI GCCn_nnn'nGGC Cuts at: 0 2198 2820 Size: 2198 622 BglII A'GATC_T Cuts at: 0 681 2820 Size: 681 2139 BlpI GC'TnA_GC Cuts at: 0 2159 2820 Size: 2159 661 Bme1580I G_kGCm'C Cuts at: 0 1126 1213 1646 2045 2255 2600 2759 2820 Size: 1126 87 433 399 210 345 159 61 Fragments arranged by size: 1126 433 399 345 210 159 87 61 BmgBI CAC'GTC Cuts at: 0 438 2457 2820 Size: 438 2019 363 Fragments arranged by size: 2019 438 363 BmrI ACTGGGnnnn_n' Cuts at: 0 1753 2224 2820 Size: 1753 471 596 Fragments arranged by size: 1753 596 471 BmtI G_CTAG'C Cuts at: 0 643 2820 Size: 643 2177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2659 2820 Size: 2659 161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 1176 1260 1326 1434 2013 2820 Size: 571 605 84 66 108 579 807 Fragments arranged by size: 807 605 579 571 108 84 66 Bpu10I CC'TnA_GC Cuts at: 0 277 1118 2820 Size: 277 841 1702 Fragments arranged by size: 1702 841 277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 301 1488 1839 2660 2820 Size: 238 63 1187 351 821 160 Fragments arranged by size: 1187 821 351 238 160 63 BsaI GGTCTCn'nnnn_ Cuts at: 0 1807 1815 2820 Size: 1807 8 1005 Fragments arranged by size: 1807 1005 8 BsaAI yAC'GTr Cuts at: 0 2066 2820 Size: 2066 754 BsaHI Gr'CG_yC Cuts at: 0 1245 2820 Size: 1245 1575 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 899 929 1458 1488 1606 1636 2006 2036 Size: 899 30 529 30 118 30 370 30 Cuts at: 2036 2820 Size: 784 Fragments arranged by size: 899 784 529 370 118 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 106 109 112 1026 1179 1309 1406 Size: 103 3 3 3 914 153 130 97 Cuts at: 1406 2230 2674 2820 Size: 824 444 146 Fragments arranged by size: 914 824 444 153 146 130 103 97 3 3 3 BseYI C'CCAG_C Cuts at: 0 429 711 1238 1432 1727 2165 2290 2820 Size: 429 282 527 194 295 438 125 530 Fragments arranged by size: 530 527 438 429 295 282 194 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1560 2820 Size: 1560 1260 BsiEI CG_ry'CG Cuts at: 0 20 2820 Size: 20 2800 BsiHKAI G_wGCw'C Cuts at: 0 1340 1646 2045 2142 2462 2820 Size: 1340 306 399 97 320 358 Fragments arranged by size: 1340 399 358 320 306 97 BsmI GAATG_Cn' Cuts at: 0 2052 2820 Size: 2052 768 Bsp1286I G_dGCh'C Cuts at: 0 924 1126 1213 1340 1646 1837 2045 2142 Size: 924 202 87 127 306 191 208 97 Cuts at: 2142 2165 2255 2462 2532 2600 2759 2820 Size: 23 90 207 70 68 159 61 Fragments arranged by size: 924 306 208 207 202 191 159 127 97 90 87 70 68 61 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 842 1135 2333 2820 Size: 842 293 1198 487 Fragments arranged by size: 1198 842 487 293 BsrBI CCG'CTC Cuts at: 0 1263 1414 2820 Size: 1263 151 1406 Fragments arranged by size: 1406 1263 151 BsrDI GCAATG_nn' Cuts at: 0 372 2820 Size: 372 2448 BsrFI r'CCGG_y Cuts at: 0 19 2820 Size: 19 2801 BstAPI GCAn_nnn'nTGC Cuts at: 0 2049 2820 Size: 2049 771 BstXI CCAn_nnnn'nTGG Cuts at: 0 2697 2820 Size: 2697 123 BstYI r'GATC_y Cuts at: 0 681 1023 2820 Size: 681 342 1797 Fragments arranged by size: 1797 681 342 Bsu36I CC'TnA_GG Cuts at: 0 295 2820 Size: 295 2525 BtgI C'CryG_G Cuts at: 0 31 2237 2397 2820 Size: 31 2206 160 423 Fragments arranged by size: 2206 423 160 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 296 592 1559 2820 Size: 296 296 967 1261 Fragments arranged by size: 1261 967 296 296 BtsI GCAGTG_nn' Cuts at: 0 1904 2820 Size: 1904 916 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1893 1928 2820 Size: 1893 35 892 Fragments arranged by size: 1893 892 35 DraIII CAC_nnn'GTG Cuts at: 0 1650 2343 2820 Size: 1650 693 477 Fragments arranged by size: 1650 693 477 DrdI GACnn_nn'nnGTC Cuts at: 0 1729 2820 Size: 1729 1091 EaeI y'GGCC_r Cuts at: 0 17 224 1254 2234 2552 2687 2820 Size: 17 207 1030 980 318 135 133 Fragments arranged by size: 1030 980 318 207 135 133 17 EagI C'GGCC_G Cuts at: 0 17 2820 Size: 17 2803 EarI CTCTTCn'nnn_ Cuts at: 0 127 331 368 395 473 1144 1515 2287 Size: 127 204 37 27 78 671 371 772 Cuts at: 2287 2651 2652 2820 Size: 364 1 168 Fragments arranged by size: 772 671 371 364 204 168 127 78 37 27 1 EcoICRI GAG'CTC Cuts at: 0 1338 2820 Size: 1338 1482 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 571 991 1176 1260 1326 1434 1966 Size: 355 216 420 185 84 66 108 532 Cuts at: 1966 2013 2092 2602 2767 2820 Size: 47 79 510 165 53 Fragments arranged by size: 532 510 420 355 216 185 165 108 84 79 66 53 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 2820 Size: 319 2501 EcoO109I rG'GnC_Cy Cuts at: 0 1209 1454 1479 2820 Size: 1209 245 25 1341 Fragments arranged by size: 1341 1209 245 25 EcoRI G'AATT_C Cuts at: 0 2446 2820 Size: 2446 374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2170 2820 Size: 2170 650 FspI TGC'GCA Cuts at: 0 49 2820 Size: 49 2771 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1008 1040 2006 2038 2420 2452 2658 2690 Size: 1008 32 966 32 382 32 206 32 Cuts at: 2690 2820 Size: 130 Fragments arranged by size: 1008 966 382 206 130 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1485 2820 Size: 1485 1335 MscI TGG'CCA Cuts at: 0 226 2554 2820 Size: 226 2328 266 Fragments arranged by size: 2328 266 226 MslI CAynn'nnrTG Cuts at: 0 2627 2820 Size: 2627 193 MspA1I CmG'CkG Cuts at: 0 33 1322 1495 1990 2169 2319 2820 Size: 33 1289 173 495 179 150 501 Fragments arranged by size: 1289 501 495 179 173 150 33 NaeI GCC'GGC Cuts at: 0 21 2820 Size: 21 2799 NcoI C'CATG_G Cuts at: 0 2397 2820 Size: 2397 423 NgoMIV G'CCGG_C Cuts at: 0 19 2820 Size: 19 2801 NheI G'CTAG_C Cuts at: 0 639 2820 Size: 639 2181 NspI r_CATG'y Cuts at: 0 691 2820 Size: 691 2129 PfoI T'CCnGG_A Cuts at: 0 1026 1450 2820 Size: 1026 424 1370 Fragments arranged by size: 1370 1026 424 PmlI CAC'GTG Cuts at: 0 2066 2820 Size: 2066 754 PpuMI rG'GwC_Cy Cuts at: 0 1454 1479 2820 Size: 1454 25 1341 Fragments arranged by size: 1454 1341 25 PsiI TTA'TAA Cuts at: 0 262 1289 2820 Size: 262 1027 1531 Fragments arranged by size: 1531 1027 262 PspOMI G'GGCC_C Cuts at: 0 1209 2596 2755 2820 Size: 1209 1387 159 65 Fragments arranged by size: 1387 1209 159 65 PstI C_TGCA'G Cuts at: 0 181 673 839 929 1132 2820 Size: 181 492 166 90 203 1688 Fragments arranged by size: 1688 492 203 181 166 90 PvuII CAG'CTG Cuts at: 0 1990 2169 2319 2820 Size: 1990 179 150 501 Fragments arranged by size: 1990 501 179 150 SacI G_AGCT'C Cuts at: 0 1340 2820 Size: 1340 1480 SacII CC_GC'GG Cuts at: 0 34 2820 Size: 34 2786 SapI GCTCTTCn'nnn_ Cuts at: 0 127 1515 2820 Size: 127 1388 1305 Fragments arranged by size: 1388 1305 127 SbfI CC_TGCA'GG Cuts at: 0 1132 2820 Size: 1132 1688 SexAI A'CCwGG_T Cuts at: 0 767 2820 Size: 767 2053 SfcI C'TryA_G Cuts at: 0 177 669 835 925 955 1128 2820 Size: 177 492 166 90 30 173 1692 Fragments arranged by size: 1692 492 177 173 166 90 30 SmlI C'TyrA_G Cuts at: 0 253 316 737 875 1503 1818 2675 2820 Size: 253 63 421 138 628 315 857 145 Fragments arranged by size: 857 628 421 315 253 145 138 63 SphI G_CATG'C Cuts at: 0 691 2820 Size: 691 2129 StyI C'CwwG_G Cuts at: 0 62 227 1004 1998 2397 2820 Size: 62 165 777 994 399 423 Fragments arranged by size: 994 777 423 399 165 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 421 2820 Size: 421 2399 TatI w'GTAC_w Cuts at: 0 448 2820 Size: 448 2372 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1446 1478 1606 1638 2820 Size: 1446 32 128 32 1182 Fragments arranged by size: 1446 1182 128 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1759 2368 2820 Size: 1759 609 452 Fragments arranged by size: 1759 609 452 XbaI T'CTAG_A Cuts at: 0 1059 2820 Size: 1059 1761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1701 2380 2820 Size: 1701 679 440 Fragments arranged by size: 1701 679 440 XhoI C'TCGA_G Cuts at: 0 737 875 2820 Size: 737 138 1945 Fragments arranged by size: 1945 737 138 XmnI GAAnn'nnTTC Cuts at: 0 586 778 2820 Size: 586 192 2042 Fragments arranged by size: 2042 586 192 ZraI GAC'GTC Cuts at: 0 1246 2820 Size: 1246 1574 Enzymes that do cut: AarI AatII AccI AclI AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HindIII MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PfoI PmlI PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SmlI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BclI BfrBI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI EciI EcoRV FseI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PspXI PsrI PvuI RsrII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XmaI