(Linear) (Six-base) MAPSORT of: KIEE2553.seq  Check: 275  from: 1  to: 774



KIEE2553.orf

 With 182 enzymes: SgfI * 

                        December 15, 2014 12:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     774 
   Size:         92     682
 
AflIII A'CryG_T

Cuts at:      0     350     774 
   Size:        350     424
 
AleI CACnn'nnGTG

Cuts at:      0     353     774 
   Size:        353     421
 
ApoI r'AATT_y

Cuts at:      0     560     774 
   Size:        560     214
 
AvaI C'yCGr_G

Cuts at:      0      40     674     774 
   Size:         40     634     100

  Fragments arranged by size: 

                 634     100      40
 
BanII G_rGCy'C

Cuts at:      0      64     232     774 
   Size:         64     168     542

  Fragments arranged by size: 

                 542     168      64
 
BglII A'GATC_T

Cuts at:      0     532     603     774 
   Size:        532      71     171

  Fragments arranged by size: 

                 532     171      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     275     774 
   Size:        275     499
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     774 
   Size:        379     395
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     225     774 
   Size:        225     549
 
BsiEI CG_ry'CG

Cuts at:      0     420     774 
   Size:        420     354
 
BsmI GAATG_Cn'

Cuts at:      0     530     774 
   Size:        530     244
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     232     774 
   Size:         64     168     542

  Fragments arranged by size: 

                 542     168      64
 
BsrFI r'CCGG_y

Cuts at:      0      46     774 
   Size:         46     728
 
BstYI r'GATC_y

Cuts at:      0     431     532     603     774 
   Size:        431     101      71     171

  Fragments arranged by size: 

                 431     171     101      71
 
Bsu36I CC'TnA_GG

Cuts at:      0     327     774 
   Size:        327     447
 
EaeI y'GGCC_r

Cuts at:      0     617     774 
   Size:        617     157
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     774 
   Size:         68     706
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     774 
   Size:         92     682
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     390     774 
   Size:        390     384
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     595     627     774 
   Size:        310      32     253      32     147

  Fragments arranged by size: 

                 310     253     147      32      32
 
HindIII A'AGCT_T

Cuts at:      0     141     774 
   Size:        141     633
 
MluI A'CGCG_T

Cuts at:      0     350     774 
   Size:        350     424
 
MscI TGG'CCA

Cuts at:      0     619     774 
   Size:        619     155
 
MslI CAynn'nnrTG

Cuts at:      0     353     774 
   Size:        353     421
 
MspA1I CmG'CkG

Cuts at:      0     425     774 
   Size:        425     349
 
NaeI GCC'GGC

Cuts at:      0      48     774 
   Size:         48     726
 
NgoMIV G'CCGG_C

Cuts at:      0      46     774 
   Size:         46     728
 
PspXI vC'TCGA_Gb

Cuts at:      0      40     774 
   Size:         40     734
 
PstI C_TGCA'G

Cuts at:      0     603     774 
   Size:        603     171
 
PvuII CAG'CTG

Cuts at:      0     425     774 
   Size:        425     349
 
SfcI C'TryA_G

Cuts at:      0     599     774 
   Size:        599     175
 
SmaI CCC'GGG

Cuts at:      0     676     774 
   Size:        676      98
 
SmlI C'TyrA_G

Cuts at:      0      40     394     774 
   Size:         40     354     380

  Fragments arranged by size: 

                 380     354      40
 
StuI AGG'CCT

Cuts at:      0     325     637     774 
   Size:        325     312     137

  Fragments arranged by size: 

                 325     312     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     774 
   Size:        186     588
 
XbaI T'CTAG_A

Cuts at:      0      54     774 
   Size:         54     720
 
XhoI C'TCGA_G

Cuts at:      0      40     774 
   Size:         40     734
 
XmaI C'CCGG_G

Cuts at:      0     674     774 
   Size:        674     100
 
XmnI GAAnn'nnTTC

Cuts at:      0     649     774 
   Size:        649     125
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AvaI    BanII    BglII     BmrI
    BpuEI    BseRI    BsiEI     BsmI Bsp1286I    BsrFI    BstYI   Bsu36I
     EaeI     EarI  Eco57MI     FalI    Hin4I  HindIII     MluI     MscI
     MslI   MspA1I     NaeI   NgoMIV    PspXI     PstI    PvuII     SfcI
     SmaI     SmlI     StuI    TaqII     XbaI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MmeI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
     TstI  Tth111I     XcmI     ZraI