(Linear) (Six-base) MAPSORT of: KIEE2554.seq Check: 4043 from: 1 to: 1083 KIEE2554.orf With 182 enzymes: SgfI * December 15, 2014 12:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 544 792 954 1083 Size: 544 248 162 129 Fragments arranged by size: 544 248 162 129 AleI CACnn'nnGTG Cuts at: 0 257 757 1083 Size: 257 500 326 Fragments arranged by size: 500 326 257 ApaLI G'TGCA_C Cuts at: 0 727 750 856 1083 Size: 727 23 106 227 Fragments arranged by size: 727 227 106 23 ApoI r'AATT_y Cuts at: 0 997 1083 Size: 997 86 BanI G'GyrC_C Cuts at: 0 624 1083 Size: 624 459 BanII G_rGCy'C Cuts at: 0 78 86 145 779 1083 Size: 78 8 59 634 304 Fragments arranged by size: 634 304 78 59 8 BbeI G_GCGC'C Cuts at: 0 628 1083 Size: 628 455 BbsI GAAGACnn'nnnn_ Cuts at: 0 726 1083 Size: 726 357 BfrBI ATG'CAT Cuts at: 0 268 1083 Size: 268 815 BglI GCCn_nnn'nGGC Cuts at: 0 880 1083 Size: 880 203 Bme1580I G_kGCm'C Cuts at: 0 731 754 860 1083 Size: 731 23 106 223 Fragments arranged by size: 731 223 106 23 BmgBI CAC'GTC Cuts at: 0 732 1083 Size: 732 351 BmrI ACTGGGnnnn_n' Cuts at: 0 415 1083 Size: 415 668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 1083 Size: 337 746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1083 Size: 829 254 BsaI GGTCTCn'nnnn_ Cuts at: 0 757 1083 Size: 757 326 BsaHI Gr'CG_yC Cuts at: 0 625 1083 Size: 625 458 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 58 135 1083 Size: 55 3 77 948 Fragments arranged by size: 948 77 55 3 BseYI C'CCAG_C Cuts at: 0 802 1083 Size: 802 281 BsiHKAI G_wGCw'C Cuts at: 0 86 145 731 754 779 860 1083 Size: 86 59 586 23 25 81 223 Fragments arranged by size: 586 223 86 81 59 25 23 BsiWI C'GTAC_G Cuts at: 0 298 1083 Size: 298 785 Bsp1286I G_dGCh'C Cuts at: 0 78 86 145 731 754 779 860 1083 Size: 78 8 59 586 23 25 81 223 Fragments arranged by size: 586 223 81 78 59 25 23 8 BsrDI GCAATG_nn' Cuts at: 0 1052 1083 Size: 1052 31 BsrFI r'CCGG_y Cuts at: 0 604 1083 Size: 604 479 BssSI C'ACGA_G Cuts at: 0 859 1083 Size: 859 224 BstAPI GCAn_nnn'nTGC Cuts at: 0 371 1083 Size: 371 712 BstEII G'GTnAC_C Cuts at: 0 834 1083 Size: 834 249 Bsu36I CC'TnA_GG Cuts at: 0 26 1083 Size: 26 1057 BtsI GCAGTG_nn' Cuts at: 0 157 930 1083 Size: 157 773 153 Fragments arranged by size: 773 157 153 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 906 941 1083 Size: 906 35 142 Fragments arranged by size: 906 142 35 EaeI y'GGCC_r Cuts at: 0 515 1058 1083 Size: 515 543 25 Fragments arranged by size: 543 515 25 EarI CTCTTCn'nnn_ Cuts at: 0 32 451 823 1083 Size: 32 419 372 260 Fragments arranged by size: 419 372 260 32 EcoICRI GAG'CTC Cuts at: 0 84 143 777 1083 Size: 84 59 634 306 Fragments arranged by size: 634 306 84 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 544 792 954 1083 Size: 337 207 248 162 129 Fragments arranged by size: 337 248 207 162 129 EcoO109I rG'GnC_Cy Cuts at: 0 191 1083 Size: 191 892 EcoRV GAT'ATC Cuts at: 0 978 1083 Size: 978 105 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 306 1083 Size: 306 777 FspI TGC'GCA Cuts at: 0 952 1083 Size: 952 131 HaeII r_GCGC'y Cuts at: 0 628 1083 Size: 628 455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 178 210 226 258 1083 Size: 178 32 16 32 825 Fragments arranged by size: 825 178 32 32 16 HincII GTy'rAC Cuts at: 0 894 1083 Size: 894 189 KasI G'GCGC_C Cuts at: 0 624 1083 Size: 624 459 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 418 1083 Size: 418 665 MslI CAynn'nnrTG Cuts at: 0 257 269 757 1083 Size: 257 12 488 326 Fragments arranged by size: 488 326 257 12 MspA1I CmG'CkG Cuts at: 0 341 743 1083 Size: 341 402 340 Fragments arranged by size: 402 341 340 NarI GG'CG_CC Cuts at: 0 625 1083 Size: 625 458 NsiI A_TGCA'T Cuts at: 0 270 1083 Size: 270 813 NspI r_CATG'y Cuts at: 0 106 272 414 1083 Size: 106 166 142 669 Fragments arranged by size: 669 166 142 106 PfoI T'CCnGG_A Cuts at: 0 32 136 1083 Size: 32 104 947 Fragments arranged by size: 947 104 32 PstI C_TGCA'G Cuts at: 0 176 1083 Size: 176 907 PvuII CAG'CTG Cuts at: 0 341 1083 Size: 341 742 SacI G_AGCT'C Cuts at: 0 86 145 779 1083 Size: 86 59 634 304 Fragments arranged by size: 634 304 86 59 ScaI AGT'ACT Cuts at: 0 379 1083 Size: 379 704 SexAI A'CCwGG_T Cuts at: 0 830 1083 Size: 830 253 SfcI C'TryA_G Cuts at: 0 172 1083 Size: 172 911 SfoI GGC'GCC Cuts at: 0 626 1083 Size: 626 457 SmlI C'TyrA_G Cuts at: 0 844 1083 Size: 844 239 SphI G_CATG'C Cuts at: 0 106 1083 Size: 106 977 StuI AGG'CCT Cuts at: 0 529 1083 Size: 529 554 TatI w'GTAC_w Cuts at: 0 377 500 1083 Size: 377 123 583 Fragments arranged by size: 583 377 123 XmnI GAAnn'nnTTC Cuts at: 0 529 565 1083 Size: 529 36 518 Fragments arranged by size: 529 518 36 Enzymes that do cut: AcuI AleI ApaLI ApoI BanI BanII BbeI BbsI BfrBI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsiWI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII Bsu36I BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI NsiI NspI PfoI PstI PvuII SacI ScaI SexAI SfcI SfoI SmlI SphI StuI TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglII BlpI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BstBI BstXI BstYI BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspAI HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI