(Linear) (Six-base) MAPSORT of: KIEE2555.seq Check: 9470 from: 1 to: 1368 KIEE2555.orf With 182 enzymes: SgfI * December 15, 2014 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1251 1368 Size: 1251 117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 402 667 827 1368 Size: 402 265 160 541 Fragments arranged by size: 541 402 265 160 AfeI AGC'GCT Cuts at: 0 344 881 1368 Size: 344 537 487 Fragments arranged by size: 537 487 344 AlwNI CAG_nnn'CTG Cuts at: 0 504 702 1266 1368 Size: 504 198 564 102 Fragments arranged by size: 564 504 198 102 AvaI C'yCGr_G Cuts at: 0 181 1368 Size: 181 1187 BamHI G'GATC_C Cuts at: 0 155 1368 Size: 155 1213 BanI G'GyrC_C Cuts at: 0 70 457 1251 1291 1368 Size: 70 387 794 40 77 Fragments arranged by size: 794 387 77 70 40 BanII G_rGCy'C Cuts at: 0 367 722 824 1368 Size: 367 355 102 544 Fragments arranged by size: 544 367 355 102 BbeI G_GCGC'C Cuts at: 0 1255 1295 1368 Size: 1255 40 73 Fragments arranged by size: 1255 73 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 840 849 891 912 1368 Size: 840 9 42 21 456 Fragments arranged by size: 840 456 42 21 9 BbvCI CC'TCA_GC Cuts at: 0 490 1368 Size: 490 878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 426 460 553 587 1368 Size: 426 34 93 34 781 Fragments arranged by size: 781 426 93 34 34 BglII A'GATC_T Cuts at: 0 866 1368 Size: 866 502 BlpI GC'TnA_GC Cuts at: 0 716 1368 Size: 716 652 Bme1580I G_kGCm'C Cuts at: 0 73 460 1118 1142 1368 Size: 73 387 658 24 226 Fragments arranged by size: 658 387 226 73 24 BmrI ACTGGGnnnn_n' Cuts at: 0 743 1368 Size: 743 625 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 656 1357 1368 Size: 656 701 11 Fragments arranged by size: 701 656 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 724 811 1368 Size: 709 15 87 557 Fragments arranged by size: 709 557 87 15 Bpu10I CC'TnA_GC Cuts at: 0 160 490 631 695 1368 Size: 160 330 141 64 673 Fragments arranged by size: 673 330 160 141 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 559 700 1368 Size: 559 141 668 Fragments arranged by size: 668 559 141 BsaAI yAC'GTr Cuts at: 0 1137 1368 Size: 1137 231 BsaHI Gr'CG_yC Cuts at: 0 14 560 1252 1292 1368 Size: 14 546 692 40 76 Fragments arranged by size: 692 546 76 40 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 23 241 712 994 1368 Size: 23 218 471 282 374 Fragments arranged by size: 471 374 282 218 23 BseYI C'CCAG_C Cuts at: 0 66 224 453 508 1026 1368 Size: 66 158 229 55 518 342 Fragments arranged by size: 518 342 229 158 66 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 443 1368 Size: 443 925 BsiHKAI G_wGCw'C Cuts at: 0 166 722 1368 Size: 166 556 646 Fragments arranged by size: 646 556 166 BsmI GAATG_Cn' Cuts at: 0 293 1368 Size: 293 1075 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5 277 1368 Size: 5 272 1091 Fragments arranged by size: 1091 272 5 Bsp1286I G_dGCh'C Cuts at: 0 73 166 367 460 722 824 1118 1142 Size: 73 93 201 93 262 102 294 24 Cuts at: 1142 1368 Size: 226 Fragments arranged by size: 294 262 226 201 102 93 93 73 24 BspHI T'CATG_A Cuts at: 0 1298 1368 Size: 1298 70 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1251 1368 Size: 1251 117 BsrFI r'CCGG_y Cuts at: 0 136 1073 1288 1368 Size: 136 937 215 80 Fragments arranged by size: 937 215 136 80 BssSI C'ACGA_G Cuts at: 0 1273 1368 Size: 1273 95 BstAPI GCAn_nnn'nTGC Cuts at: 0 1266 1368 Size: 1266 102 BstEII G'GTnAC_C Cuts at: 0 942 1368 Size: 942 426 BstXI CCAn_nnnn'nTGG Cuts at: 0 390 555 1248 1368 Size: 390 165 693 120 Fragments arranged by size: 693 390 165 120 BstYI r'GATC_y Cuts at: 0 155 512 780 866 1368 Size: 155 357 268 86 502 Fragments arranged by size: 502 357 268 155 86 BtgI C'CryG_G Cuts at: 0 19 389 542 938 1368 Size: 19 370 153 396 430 Fragments arranged by size: 430 396 370 153 19 DraIII CAC_nnn'GTG Cuts at: 0 1244 1368 Size: 1244 124 DrdI GACnn_nn'nnGTC Cuts at: 0 219 1368 Size: 219 1149 EaeI y'GGCC_r Cuts at: 0 128 311 495 545 668 1057 1286 1368 Size: 128 183 184 50 123 389 229 82 Fragments arranged by size: 389 229 184 183 128 123 82 50 EarI CTCTTCn'nnn_ Cuts at: 0 247 448 490 1012 1126 1171 1357 1368 Size: 247 201 42 522 114 45 186 11 Fragments arranged by size: 522 247 201 186 114 45 42 11 EcoICRI GAG'CTC Cuts at: 0 720 1368 Size: 720 648 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 402 667 709 724 811 827 1368 Size: 402 265 42 15 87 16 541 Fragments arranged by size: 541 402 265 87 42 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 793 1354 1368 Size: 793 561 14 Fragments arranged by size: 793 561 14 EcoO109I rG'GnC_Cy Cuts at: 0 178 501 1148 1368 Size: 178 323 647 220 Fragments arranged by size: 647 323 220 178 FspI TGC'GCA Cuts at: 0 292 1368 Size: 292 1076 FspAI rTGC'GCAy Cuts at: 0 292 1368 Size: 292 1076 HaeII r_GCGC'y Cuts at: 0 346 883 1255 1295 1363 1368 Size: 346 537 372 40 68 5 Fragments arranged by size: 537 372 346 68 40 5 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 765 790 797 822 1368 Size: 765 25 7 25 546 Fragments arranged by size: 765 546 25 25 7 KasI G'GCGC_C Cuts at: 0 1251 1291 1368 Size: 1251 40 77 Fragments arranged by size: 1251 77 40 MfeI C'AATT_G Cuts at: 0 204 1368 Size: 204 1164 MscI TGG'CCA Cuts at: 0 313 547 670 1059 1368 Size: 313 234 123 389 309 Fragments arranged by size: 389 313 309 234 123 MslI CAynn'nnrTG Cuts at: 0 388 610 937 1195 1368 Size: 388 222 327 258 173 Fragments arranged by size: 388 327 258 222 173 MspA1I CmG'CkG Cuts at: 0 21 228 453 494 1368 Size: 21 207 225 41 874 Fragments arranged by size: 874 225 207 41 21 NaeI GCC'GGC Cuts at: 0 138 1075 1290 1368 Size: 138 937 215 78 Fragments arranged by size: 937 215 138 78 NarI GG'CG_CC Cuts at: 0 1252 1292 1368 Size: 1252 40 76 Fragments arranged by size: 1252 76 40 NcoI C'CATG_G Cuts at: 0 389 542 938 1368 Size: 389 153 396 430 Fragments arranged by size: 430 396 389 153 NgoMIV G'CCGG_C Cuts at: 0 136 1073 1288 1368 Size: 136 937 215 80 Fragments arranged by size: 937 215 136 80 PasI CC'CwG_GG Cuts at: 0 576 1317 1368 Size: 576 741 51 Fragments arranged by size: 741 576 51 PflMI CCAn_nnn'nTGG Cuts at: 0 504 1368 Size: 504 864 PfoI T'CCnGG_A Cuts at: 0 278 1368 Size: 278 1090 PmlI CAC'GTG Cuts at: 0 1137 1368 Size: 1137 231 PpuMI rG'GwC_Cy Cuts at: 0 178 1148 1368 Size: 178 970 220 Fragments arranged by size: 970 220 178 PvuII CAG'CTG Cuts at: 0 228 453 1368 Size: 228 225 915 Fragments arranged by size: 915 228 225 SacI G_AGCT'C Cuts at: 0 722 1368 Size: 722 646 SacII CC_GC'GG Cuts at: 0 22 1368 Size: 22 1346 SanDI GG'GwC_CC Cuts at: 0 178 1368 Size: 178 1190 SapI GCTCTTCn'nnn_ Cuts at: 0 247 1368 Size: 247 1121 SexAI A'CCwGG_T Cuts at: 0 35 1226 1368 Size: 35 1191 142 Fragments arranged by size: 1191 142 35 SfcI C'TryA_G Cuts at: 0 430 474 1368 Size: 430 44 894 Fragments arranged by size: 894 430 44 SfoI GGC'GCC Cuts at: 0 1253 1293 1368 Size: 1253 40 75 Fragments arranged by size: 1253 75 40 StyI C'CwwG_G Cuts at: 0 389 542 938 1368 Size: 389 153 396 430 Fragments arranged by size: 430 396 389 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 198 1368 Size: 198 1170 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 63 95 1368 Size: 63 32 1273 Fragments arranged by size: 1273 63 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 505 936 1368 Size: 505 431 432 Fragments arranged by size: 505 432 431 XmnI GAAnn'nnTTC Cuts at: 0 91 840 1368 Size: 91 749 528 Fragments arranged by size: 749 528 91 Enzymes that do cut: AarI AcuI AfeI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI MfeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PvuII SacI SacII SanDI SapI SexAI SfcI SfoI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglI BmgBI BmtI BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EciI EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI