Restriction map for
pF1KE2556
(Linear) (Six-base) MAPSORT of: KIEE2556.seq Check: 6195 from: 1 to: 3522
KIEE2556.orf
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1671 3522
Size: 1671 1851
AatII G_ACGT'C
Cuts at: 0 1277 3522
Size: 1277 2245
AccI GT'mk_AC
Cuts at: 0 1181 1469 3522
Size: 1181 288 2053
Fragments arranged by size:
2053 1181 288
AclI AA'CG_TT
Cuts at: 0 1813 3522
Size: 1813 1709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 302 391 475 872 2461 3151 3522
Size: 174 128 89 84 397 1589 690 371
Fragments arranged by size:
1589 690 397 371 174 128 89 84
AflII C'TTAA_G
Cuts at: 0 2710 3522
Size: 2710 812
AflIII A'CryG_T
Cuts at: 0 782 3522
Size: 782 2740
AhdI GACnn_n'nnGTC
Cuts at: 0 1656 3522
Size: 1656 1866
AleI CACnn'nnGTG
Cuts at: 0 3323 3475 3522
Size: 3323 152 47
Fragments arranged by size:
3323 152 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 3522
Size: 791 2731
AlwNI CAG_nnn'CTG
Cuts at: 0 165 197 601 3106 3501 3522
Size: 165 32 404 2505 395 21
Fragments arranged by size:
2505 404 395 165 32 21
ApaI G_GGCC'C
Cuts at: 0 2175 2261 3522
Size: 2175 86 1261
Fragments arranged by size:
2175 1261 86
ApaLI G'TGCA_C
Cuts at: 0 1187 1795 3522
Size: 1187 608 1727
Fragments arranged by size:
1727 1187 608
ApoI r'AATT_y
Cuts at: 0 496 2654 2705 3522
Size: 496 2158 51 817
Fragments arranged by size:
2158 817 496 51
AvaI C'yCGr_G
Cuts at: 0 145 1480 2174 2525 2588 3408 3522
Size: 145 1335 694 351 63 820 114
Fragments arranged by size:
1335 820 694 351 145 114 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1098 1131 1312 1345 3522
Size: 1098 33 181 33 2177
Fragments arranged by size:
2177 1098 181 33 33
BamHI G'GATC_C
Cuts at: 0 77 1163 3522
Size: 77 1086 2359
Fragments arranged by size:
2359 1086 77
BanI G'GyrC_C
Cuts at: 0 1992 2178 3173 3522
Size: 1992 186 995 349
Fragments arranged by size:
1992 995 349 186
BanII G_rGCy'C
Cuts at: 0 593 1143 1868 1928 2017 2175 2261 2279
Size: 593 550 725 60 89 158 86 18
Cuts at: 2279 2427 2498 3522
Size: 148 71 1024
Fragments arranged by size:
1024 725 593 550 158 148 89 86
71 60 18
BbeI G_GCGC'C
Cuts at: 0 1996 2182 3522
Size: 1996 186 1340
Fragments arranged by size:
1996 1340 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 860 965 3221 3522
Size: 860 105 2256 301
Fragments arranged by size:
2256 860 301 105
BbvCI CC'TCA_GC
Cuts at: 0 1217 3427 3522
Size: 1217 2210 95
Fragments arranged by size:
2210 1217 95
BciVI GTATCCnnnnn_n'
Cuts at: 0 1099 3522
Size: 1099 2423
BclI T'GATC_A
Cuts at: 0 465 3522
Size: 465 3057
BfrBI ATG'CAT
Cuts at: 0 274 3522
Size: 274 3248
BglI GCCn_nnn'nGGC
Cuts at: 0 1716 1943 3522
Size: 1716 227 1579
Fragments arranged by size:
1716 1579 227
BlpI GC'TnA_GC
Cuts at: 0 435 1927 3522
Size: 435 1492 1595
Fragments arranged by size:
1595 1492 435
Bme1580I G_kGCm'C
Cuts at: 0 1094 1191 1799 2175 2261 3522
Size: 1094 97 608 376 86 1261
Fragments arranged by size:
1261 1094 608 376 97 86
BmgBI CAC'GTC
Cuts at: 0 2289 2408 3522
Size: 2289 119 1114
Fragments arranged by size:
2289 1114 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 3161 3522
Size: 3161 361
BmtI G_CTAG'C
Cuts at: 0 430 3522
Size: 430 3092
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2453 3522
Size: 2453 1069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1960 2293 2379 3495 3522
Size: 187 1773 333 86 1116 27
Fragments arranged by size:
1773 1116 333 187 86 27
Bpu10I CC'TnA_GC
Cuts at: 0 671 1217 1540 3427 3522
Size: 671 546 323 1887 95
Fragments arranged by size:
1887 671 546 323 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1939 2097 3205 3522
Size: 1939 158 1108 317
Fragments arranged by size:
1939 1108 317 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 826 2877 3522
Size: 826 2051 645
Fragments arranged by size:
2051 826 645
BsaAI yAC'GTr
Cuts at: 0 1011 2191 3522
Size: 1011 1180 1331
Fragments arranged by size:
1331 1180 1011
BsaBI GATnn'nnATC
Cuts at: 0 3231 3370 3522
Size: 3231 139 152
Fragments arranged by size:
3231 152 139
BsaHI Gr'CG_yC
Cuts at: 0 1274 1993 2108 2179 3264 3522
Size: 1274 719 115 71 1085 258
Fragments arranged by size:
1274 1085 719 258 115 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 873 903 3522
Size: 873 30 2619
Fragments arranged by size:
2619 873 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 844 981 2077 2149 2152 2155 2173 2176
Size: 844 137 1096 72 3 3 18 3
Cuts at: 2176 3013 3522
Size: 837 509
Fragments arranged by size:
1096 844 837 509 137 72 18 3
3 3
BseYI C'CCAG_C
Cuts at: 0 1001 2018 2023 2348 3328 3522
Size: 1001 1017 5 325 980 194
Fragments arranged by size:
1017 1001 980 325 194 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 2171 2248 3472 3522
Size: 1879 292 77 1224 50
Fragments arranged by size:
1879 1224 292 77 50
BsiEI CG_ry'CG
Cuts at: 0 1161 1520 2339 3522
Size: 1161 359 819 1183
Fragments arranged by size:
1183 1161 819 359
BsiHKAI G_wGCw'C
Cuts at: 0 130 1191 1496 1799 2740 3522
Size: 130 1061 305 303 941 782
Fragments arranged by size:
1061 941 782 305 303 130
BsiWI C'GTAC_G
Cuts at: 0 1439 3522
Size: 1439 2083
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1568 1664 2010 2357 3522
Size: 1568 96 346 347 1165
Fragments arranged by size:
1568 1165 347 346 96
Bsp1286I G_dGCh'C
Cuts at: 0 130 593 1094 1143 1191 1496 1799 1868
Size: 130 463 501 49 48 305 303 69
Cuts at: 1868 1928 2017 2175 2261 2279 2427 2498 2740
Size: 60 89 158 86 18 148 71 242
Cuts at: 2740 3522
Size: 782
Fragments arranged by size:
782 501 463 305 303 242 158 148
130 89 86 71 69 60 49 48
18
BspHI T'CATG_A
Cuts at: 0 1277 3368 3522
Size: 1277 2091 154
Fragments arranged by size:
2091 1277 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 938 1671 3522
Size: 938 733 1851
Fragments arranged by size:
1851 938 733
BsrBI CCG'CTC
Cuts at: 0 1587 2168 3522
Size: 1587 581 1354
Fragments arranged by size:
1587 1354 581
BsrDI GCAATG_nn'
Cuts at: 0 773 2987 3522
Size: 773 2214 535
Fragments arranged by size:
2214 773 535
BsrFI r'CCGG_y
Cuts at: 0 224 1688 2451 3522
Size: 224 1464 763 1071
Fragments arranged by size:
1464 1071 763 224
BsrGI T'GTAC_A
Cuts at: 0 617 3479 3522
Size: 617 2862 43
Fragments arranged by size:
2862 617 43
BssHII G'CGCG_C
Cuts at: 0 1879 2194 3522
Size: 1879 315 1328
Fragments arranged by size:
1879 1328 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 62 1848 3522
Size: 62 1786 1674
Fragments arranged by size:
1786 1674 62
BstYI r'GATC_y
Cuts at: 0 77 1163 1484 3248 3522
Size: 77 1086 321 1764 274
Fragments arranged by size:
1764 1086 321 274 77
BtgI C'CryG_G
Cuts at: 0 119 152 1457 1595 1835 1932 3522
Size: 119 33 1305 138 240 97 1590
Fragments arranged by size:
1590 1305 240 138 119 97 33
BtsI GCAGTG_nn'
Cuts at: 0 4 1182 1524 3318 3330 3522
Size: 4 1178 342 1794 12 192
Fragments arranged by size:
1794 1178 342 192 12 4
ClaI AT'CG_AT
Cuts at: 0 2775 3522
Size: 2775 747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1013 1048 3522
Size: 1013 35 2474
Fragments arranged by size:
2474 1013 35
DraIII CAC_nnn'GTG
Cuts at: 0 1806 3522
Size: 1806 1716
EaeI y'GGCC_r
Cuts at: 0 132 1517 1690 1919 2085 3031 3522
Size: 132 1385 173 229 166 946 491
Fragments arranged by size:
1385 946 491 229 173 166 132
EagI C'GGCC_G
Cuts at: 0 1517 3522
Size: 1517 2005
EarI CTCTTCn'nnn_
Cuts at: 0 650 2299 2990 3010 3239 3522
Size: 650 1649 691 20 229 283
Fragments arranged by size:
1649 691 650 283 229 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2083 3522
Size: 2083 1439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 187 302 391 475 872 1960 2293
Size: 174 13 115 89 84 397 1088 333
Cuts at: 2293 2379 2461 3151 3495 3522
Size: 86 82 690 344 27
Fragments arranged by size:
1088 690 397 344 333 174 115 89
86 84 82 27 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1449 2218 2606 3069 3522
Size: 1449 769 388 463 453
Fragments arranged by size:
1449 769 463 453 388
EcoO109I rG'GnC_Cy
Cuts at: 0 1380 1944 2171 2223 2258 3522
Size: 1380 564 227 52 35 1264
Fragments arranged by size:
1380 1264 564 227 52 35
EcoRI G'AATT_C
Cuts at: 0 2705 3522
Size: 2705 817
EcoRV GAT'ATC
Cuts at: 0 381 3522
Size: 381 3141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 416 2806 3522
Size: 416 2390 716
Fragments arranged by size:
2390 716 416
FspI TGC'GCA
Cuts at: 0 1634 3522
Size: 1634 1888
HaeII r_GCGC'y
Cuts at: 0 42 225 1316 1465 1996 2182 3522
Size: 42 183 1091 149 531 186 1340
Fragments arranged by size:
1340 1091 531 186 183 149 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1642 1674 2452 2484 3238 3270 3476 3508
Size: 1642 32 778 32 754 32 206 32
Cuts at: 3508 3522
Size: 14
Fragments arranged by size:
1642 778 754 206 32 32 32 32
14
HindIII A'AGCT_T
Cuts at: 0 1128 3522
Size: 1128 2394
KasI G'GCGC_C
Cuts at: 0 1992 2178 3522
Size: 1992 186 1344
Fragments arranged by size:
1992 1344 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1141 1274 2084 2369 2420 2438 3341
Size: 105 1036 133 810 285 51 18 903
Cuts at: 3341 3522
Size: 181
Fragments arranged by size:
1036 903 810 285 181 133 105 51
18
MscI TGG'CCA
Cuts at: 0 134 3033 3522
Size: 134 2899 489
Fragments arranged by size:
2899 489 134
MslI CAynn'nnrTG
Cuts at: 0 737 1040 1915 3323 3475 3522
Size: 737 303 875 1408 152 47
Fragments arranged by size:
1408 875 737 303 152 47
MspA1I CmG'CkG
Cuts at: 0 261 330 1459 1516 1638 1893 2352 3522
Size: 261 69 1129 57 122 255 459 1170
Fragments arranged by size:
1170 1129 459 261 255 122 69 57
NarI GG'CG_CC
Cuts at: 0 1993 2179 3522
Size: 1993 186 1343
Fragments arranged by size:
1993 1343 186
NdeI CA'TA_TG
Cuts at: 0 1062 3522
Size: 1062 2460
NheI G'CTAG_C
Cuts at: 0 426 3522
Size: 426 3096
NotI GC'GGCC_GC
Cuts at: 0 1517 3522
Size: 1517 2005
NsiI A_TGCA'T
Cuts at: 0 276 3522
Size: 276 3246
NspI r_CATG'y
Cuts at: 0 624 786 1393 3426 3522
Size: 624 162 607 2033 96
Fragments arranged by size:
2033 624 607 162 96
PciI A'CATG_T
Cuts at: 0 782 3522
Size: 782 2740
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 561 1938 3040 3522
Size: 165 396 1377 1102 482
Fragments arranged by size:
1377 1102 482 396 165
PfoI T'CCnGG_A
Cuts at: 0 1817 2270 3522
Size: 1817 453 1252
Fragments arranged by size:
1817 1252 453
PmlI CAC'GTG
Cuts at: 0 2191 3522
Size: 2191 1331
PpuMI rG'GwC_Cy
Cuts at: 0 2223 3522
Size: 2223 1299
PspOMI G'GGCC_C
Cuts at: 0 2171 2257 3522
Size: 2171 86 1265
Fragments arranged by size:
2171 1265 86
PspXI vC'TCGA_Gb
Cuts at: 0 145 2525 3522
Size: 145 2380 997
Fragments arranged by size:
2380 997 145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 3522
Size: 1093 32 2397
Fragments arranged by size:
2397 1093 32
PstI C_TGCA'G
Cuts at: 0 335 1643 1853 3325 3522
Size: 335 1308 210 1472 197
Fragments arranged by size:
1472 1308 335 210 197
PvuII CAG'CTG
Cuts at: 0 261 330 1638 1893 3522
Size: 261 69 1308 255 1629
Fragments arranged by size:
1629 1308 261 255 69
SacII CC_GC'GG
Cuts at: 0 1460 3522
Size: 1460 2062
SapI GCTCTTCn'nnn_
Cuts at: 0 3010 3522
Size: 3010 512
SexAI A'CCwGG_T
Cuts at: 0 346 3522
Size: 346 3176
SfcI C'TryA_G
Cuts at: 0 331 1182 1446 1470 1545 1639 1849 3321
Size: 331 851 264 24 75 94 210 1472
Cuts at: 3321 3522
Size: 201
Fragments arranged by size:
1472 851 331 264 210 201 94 75
24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1943 3522
Size: 1943 1579
SfoI GGC'GCC
Cuts at: 0 1994 2180 3522
Size: 1994 186 1342
Fragments arranged by size:
1994 1342 186
SgrAI Cr'CCGG_yG
Cuts at: 0 224 3522
Size: 224 3298
SmaI CCC'GGG
Cuts at: 0 2176 3522
Size: 2176 1346
SmlI C'TyrA_G
Cuts at: 0 145 1954 2076 2525 2588 2710 3220 3522
Size: 145 1809 122 449 63 122 510 302
Fragments arranged by size:
1809 510 449 302 145 122 122 63
SphI G_CATG'C
Cuts at: 0 1393 3522
Size: 1393 2129
SrfI GCCC'GGGC
Cuts at: 0 2176 3522
Size: 2176 1346
StuI AGG'CCT
Cuts at: 0 1284 3128 3522
Size: 1284 1844 394
Fragments arranged by size:
1844 1284 394
StyI C'CwwG_G
Cuts at: 0 681 2279 3522
Size: 681 1598 1243
Fragments arranged by size:
1598 1243 681
TatI w'GTAC_w
Cuts at: 0 617 3467 3479 3522
Size: 617 2850 12 43
Fragments arranged by size:
2850 617 43 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 873 905 2603 2635 3270 3302
Size: 781 32 60 32 1698 32 635 32
Cuts at: 3302 3522
Size: 220
Fragments arranged by size:
1698 781 635 220 60 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2227 3325 3522
Size: 2227 1098 197
Fragments arranged by size:
2227 1098 197
XhoI C'TCGA_G
Cuts at: 0 145 2525 2588 3522
Size: 145 2380 63 934
Fragments arranged by size:
2380 934 145 63
XmaI C'CCGG_G
Cuts at: 0 2174 3522
Size: 2174 1348
XmnI GAAnn'nnTTC
Cuts at: 0 2705 3522
Size: 2705 817
ZraI GAC'GTC
Cuts at: 0 1275 3522
Size: 1275 2247
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstYI BtgI BtsI ClaI CspCI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NarI NdeI NheI NotI NsiI NspI PciI
PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI PstI
PvuII SacII SapI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AloI AscI AseI AsiSI AvrII
BcgI BglII BsaWI BsmI BspEI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI DraI DrdI EcoICRI FseI
FspAI HincII HpaI KpnI MfeI MluI NaeI NcoI
NgoMIV NruI PacI PasI PmeI PpiI PshAI PsiI
PvuI RsrII SacI SalI SanDI SbfI ScaI SgfI
SnaBI SpeI SspI SwaI TaqII Tth111I XbaI