Restriction map for
pF1KE2566
(Linear) (Six-base) MAPSORT of: KIEE2566.seq Check: 2817 from: 1 to: 1710
KIEE2566.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AccI GT'mk_AC
Cuts at: 0 113 1710
Size: 113 1597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 353 526 1302 1387 1710
Size: 217 136 173 776 85 323
Fragments arranged by size:
776 323 217 173 136 85
AflII C'TTAA_G
Cuts at: 0 774 1444 1710
Size: 774 670 266
Fragments arranged by size:
774 670 266
AhdI GACnn_n'nnGTC
Cuts at: 0 321 1710
Size: 321 1389
AleI CACnn'nnGTG
Cuts at: 0 1606 1710
Size: 1606 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1222 1624 1710
Size: 1222 402 86
Fragments arranged by size:
1222 402 86
ApaLI G'TGCA_C
Cuts at: 0 1609 1710
Size: 1609 101
ApoI r'AATT_y
Cuts at: 0 370 607 808 1710
Size: 370 237 201 902
Fragments arranged by size:
902 370 237 201
BamHI G'GATC_C
Cuts at: 0 450 1710
Size: 450 1260
BanI G'GyrC_C
Cuts at: 0 1600 1710
Size: 1600 110
BanII G_rGCy'C
Cuts at: 0 257 1678 1710
Size: 257 1421 32
Fragments arranged by size:
1421 257 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 1710
Size: 267 1443
BbvCI CC'TCA_GC
Cuts at: 0 359 1710
Size: 359 1351
BclI T'GATC_A
Cuts at: 0 302 1710
Size: 302 1408
Bme1580I G_kGCm'C
Cuts at: 0 1613 1710
Size: 1613 97
BmgBI CAC'GTC
Cuts at: 0 318 1710
Size: 318 1392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1084 1598 1710
Size: 1063 21 514 112
Fragments arranged by size:
1063 514 112 21
Bpu10I CC'TnA_GC
Cuts at: 0 359 1710
Size: 359 1351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1664 1710
Size: 461 1203 46
Fragments arranged by size:
1203 461 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1538 1710
Size: 1538 172
BseYI C'CCAG_C
Cuts at: 0 458 1474 1710
Size: 458 1016 236
Fragments arranged by size:
1016 458 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1639 1710
Size: 1639 71
BsiHKAI G_wGCw'C
Cuts at: 0 257 1098 1613 1678 1710
Size: 257 841 515 65 32
Fragments arranged by size:
841 515 257 65 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 1710
Size: 82 1628
Bsp1286I G_dGCh'C
Cuts at: 0 257 1098 1613 1678 1710
Size: 257 841 515 65 32
Fragments arranged by size:
841 515 257 65 32
BspHI T'CATG_A
Cuts at: 0 185 1710
Size: 185 1525
BsrDI GCAATG_nn'
Cuts at: 0 386 631 673 1710
Size: 386 245 42 1037
Fragments arranged by size:
1037 386 245 42
BssSI C'ACGA_G
Cuts at: 0 1264 1710
Size: 1264 446
BstYI r'GATC_y
Cuts at: 0 450 1710
Size: 450 1260
BstZ17I GTA'TAC
Cuts at: 0 114 1710
Size: 114 1596
Bsu36I CC'TnA_GG
Cuts at: 0 1228 1496 1710
Size: 1228 268 214
Fragments arranged by size:
1228 268 214
BtgI C'CryG_G
Cuts at: 0 701 1710
Size: 701 1009
EarI CTCTTCn'nnn_
Cuts at: 0 1321 1532 1710
Size: 1321 211 178
Fragments arranged by size:
1321 211 178
EcoICRI GAG'CTC
Cuts at: 0 255 1676 1710
Size: 255 1421 34
Fragments arranged by size:
1421 255 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 353 526 1063 1084 1302 1387 1598
Size: 217 136 173 537 21 218 85 211
Cuts at: 1598 1710
Size: 112
Fragments arranged by size:
537 218 217 211 173 136 112 85
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1466 1710
Size: 1466 244
EcoO109I rG'GnC_Cy
Cuts at: 0 1471 1710
Size: 1471 239
EcoRI G'AATT_C
Cuts at: 0 607 808 1710
Size: 607 201 902
Fragments arranged by size:
902 607 201
EcoRV GAT'ATC
Cuts at: 0 1503 1710
Size: 1503 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1459 1710
Size: 1459 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 513 545 770 802 1710
Size: 307 32 174 32 225 32 908
Fragments arranged by size:
908 307 225 174 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 984 1710
Size: 595 389 726
Fragments arranged by size:
726 595 389
MslI CAynn'nnrTG
Cuts at: 0 391 1091 1606 1710
Size: 391 700 515 104
Fragments arranged by size:
700 515 391 104
MspA1I CmG'CkG
Cuts at: 0 53 1710
Size: 53 1657
NcoI C'CATG_G
Cuts at: 0 701 1710
Size: 701 1009
NspI r_CATG'y
Cuts at: 0 994 1710
Size: 994 716
PfoI T'CCnGG_A
Cuts at: 0 1575 1710
Size: 1575 135
PsiI TTA'TAA
Cuts at: 0 1556 1710
Size: 1556 154
PstI C_TGCA'G
Cuts at: 0 1124 1275 1710
Size: 1124 151 435
Fragments arranged by size:
1124 435 151
SacI G_AGCT'C
Cuts at: 0 257 1678 1710
Size: 257 1421 32
Fragments arranged by size:
1421 257 32
ScaI AGT'ACT
Cuts at: 0 71 1710
Size: 71 1639
SfcI C'TryA_G
Cuts at: 0 133 1120 1271 1710
Size: 133 987 151 439
Fragments arranged by size:
987 439 151 133
SmlI C'TyrA_G
Cuts at: 0 476 774 1444 1679 1710
Size: 476 298 670 235 31
Fragments arranged by size:
670 476 298 235 31
SspI AAT'ATT
Cuts at: 0 420 1710
Size: 420 1290
StyI C'CwwG_G
Cuts at: 0 701 1710
Size: 701 1009
TatI w'GTAC_w
Cuts at: 0 26 69 1710
Size: 26 43 1641
Fragments arranged by size:
1641 43 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1224 1256 1710
Size: 1224 32 454
Fragments arranged by size:
1224 454 32
XbaI T'CTAG_A
Cuts at: 0 466 1710
Size: 466 1244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1332 1710
Size: 1332 378
XmnI GAAnn'nnTTC
Cuts at: 0 878 1624 1710
Size: 878 746 86
Fragments arranged by size:
878 746 86
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI ApaLI ApoI
BamHI BanI BanII BbsI BbvCI BclI Bme1580I BmgBI
BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BsrDI BssSI BstYI BstZ17I Bsu36I BtgI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I MmeI MslI MspA1I NcoI NspI PfoI PsiI
PstI SacI ScaI SfcI SmlI SspI StyI TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BfrBI BglI BglII BlpI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstXI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XhoI XmaI ZraI