Restriction map for
pF1KE2569
(Linear) (Six-base) MAPSORT of: KIEE2569.seq Check: 2695 from: 1 to: 3273
KIEE2569.orf
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AccI GT'mk_AC
Cuts at: 0 2463 3273
Size: 2463 810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1413 1739 2302 2425 2638 3232 3273
Size: 631 782 326 563 123 213 594 41
Fragments arranged by size:
782 631 594 563 326 213 123 41
AfeI AGC'GCT
Cuts at: 0 2856 3273
Size: 2856 417
AflII C'TTAA_G
Cuts at: 0 1617 3273
Size: 1617 1656
AflIII A'CryG_T
Cuts at: 0 1091 1269 2090 3164 3273
Size: 1091 178 821 1074 109
Fragments arranged by size:
1091 1074 821 178 109
AleI CACnn'nnGTG
Cuts at: 0 511 2917 3273
Size: 511 2406 356
Fragments arranged by size:
2406 511 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 930 3273
Size: 930 2343
AlwNI CAG_nnn'CTG
Cuts at: 0 573 789 1368 1953 3273
Size: 573 216 579 585 1320
Fragments arranged by size:
1320 585 579 573 216
ApaI G_GGCC'C
Cuts at: 0 799 3273
Size: 799 2474
ApaLI G'TGCA_C
Cuts at: 0 3168 3273
Size: 3168 105
ApoI r'AATT_y
Cuts at: 0 700 1004 2196 2509 2692 2791 3273
Size: 700 304 1192 313 183 99 482
Fragments arranged by size:
1192 700 482 313 304 183 99
AseI AT'TA_AT
Cuts at: 0 1691 2071 3273
Size: 1691 380 1202
Fragments arranged by size:
1691 1202 380
AvaI C'yCGr_G
Cuts at: 0 86 202 921 2004 2887 3273
Size: 86 116 719 1083 883 386
Fragments arranged by size:
1083 883 719 386 116 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1565 1598 3273
Size: 1565 33 1675
Fragments arranged by size:
1675 1565 33
BanI G'GyrC_C
Cuts at: 0 20 385 2911 3273
Size: 20 365 2526 362
Fragments arranged by size:
2526 365 362 20
BanII G_rGCy'C
Cuts at: 0 169 799 953 1417 2419 2888 3039 3273
Size: 169 630 154 464 1002 469 151 234
Fragments arranged by size:
1002 630 469 464 234 169 154 151
BbeI G_GCGC'C
Cuts at: 0 24 3273
Size: 24 3249
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 183 224 1727 2413 3220 3273
Size: 135 48 41 1503 686 807 53
Fragments arranged by size:
1503 807 686 135 53 48 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 355 3154 3273
Size: 355 2799 119
Fragments arranged by size:
2799 355 119
BclI T'GATC_A
Cuts at: 0 584 966 1388 2291 3273
Size: 584 382 422 903 982
Fragments arranged by size:
982 903 584 422 382
BfrBI ATG'CAT
Cuts at: 0 992 3273
Size: 992 2281
BglI GCCn_nnn'nGGC
Cuts at: 0 2892 3273
Size: 2892 381
BlpI GC'TnA_GC
Cuts at: 0 517 2594 3273
Size: 517 2077 679
Fragments arranged by size:
2077 679 517
Bme1580I G_kGCm'C
Cuts at: 0 799 2864 3172 3273
Size: 799 2065 308 101
Fragments arranged by size:
2065 799 308 101
BmgBI CAC'GTC
Cuts at: 0 1270 2349 2712 3273
Size: 1270 1079 363 561
Fragments arranged by size:
1270 1079 561 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1274 1315 2587 3273
Size: 1148 126 41 1272 686
Fragments arranged by size:
1272 1148 686 126 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 3273
Size: 1696 1577
BsaI GGTCTCn'nnnn_
Cuts at: 0 2376 2530 3273
Size: 2376 154 743
Fragments arranged by size:
2376 743 154
BsaAI yAC'GTr
Cuts at: 0 2589 3167 3273
Size: 2589 578 106
Fragments arranged by size:
2589 578 106
BsaBI GATnn'nnATC
Cuts at: 0 277 2290 3273
Size: 277 2013 983
Fragments arranged by size:
2013 983 277
BsaHI Gr'CG_yC
Cuts at: 0 21 470 3273
Size: 21 449 2803
Fragments arranged by size:
2803 449 21
BsaWI w'CCGG_w
Cuts at: 0 2679 3100 3273
Size: 2679 421 173
Fragments arranged by size:
2679 421 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 378 404 408 917 947 2109 2139
Size: 374 4 26 4 509 30 1162 30
Cuts at: 2139 2953 2983 3273
Size: 814 30 290
Fragments arranged by size:
1162 814 509 374 290 30 30 30
26 4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 486 489 1339 2542 2641 3145 3273
Size: 64 422 3 850 1203 99 504 128
Fragments arranged by size:
1203 850 504 422 128 99 64 3
BseYI C'CCAG_C
Cuts at: 0 1418 2863 3273
Size: 1418 1445 410
Fragments arranged by size:
1445 1418 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1870 3273
Size: 1870 1403
BsiHKAI G_wGCw'C
Cuts at: 0 557 953 1301 2600 2876 3039 3172 3273
Size: 557 396 348 1299 276 163 133 101
Fragments arranged by size:
1299 557 396 348 276 163 133 101
BsmI GAATG_Cn'
Cuts at: 0 994 1158 2231 2737 3273
Size: 994 164 1073 506 536
Fragments arranged by size:
1073 994 536 506 164
BsmBI CGTCTCn'nnnn_
Cuts at: 0 62 396 461 3127 3273
Size: 62 334 65 2666 146
Fragments arranged by size:
2666 334 146 65 62
Bsp1286I G_dGCh'C
Cuts at: 0 169 557 799 953 1301 1417 2419 2600
Size: 169 388 242 154 348 116 1002 181
Cuts at: 2600 2864 2876 2888 3039 3172 3273
Size: 264 12 12 151 133 101
Fragments arranged by size:
1002 388 348 264 242 181 169 154
151 133 116 101 12 12
BspEI T'CCGG_A
Cuts at: 0 2679 3273
Size: 2679 594
BspHI T'CATG_A
Cuts at: 0 2294 3273
Size: 2294 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 3273
Size: 41 3232
BsrBI CCG'CTC
Cuts at: 0 44 135 723 2523 2892 3273
Size: 44 91 588 1800 369 381
Fragments arranged by size:
1800 588 381 369 91 44
BsrDI GCAATG_nn'
Cuts at: 0 476 1711 1963 2012 3273
Size: 476 1235 252 49 1261
Fragments arranged by size:
1261 1235 476 252 49
BsrFI r'CCGG_y
Cuts at: 0 744 3273
Size: 744 2529
BsrGI T'GTAC_A
Cuts at: 0 440 3273
Size: 440 2833
BssSI C'ACGA_G
Cuts at: 0 1858 3273
Size: 1858 1415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2901 3273
Size: 2901 372
BstEII G'GTnAC_C
Cuts at: 0 3123 3273
Size: 3123 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1245 2393 3090 3273
Size: 1245 1148 697 183
Fragments arranged by size:
1245 1148 697 183
BstYI r'GATC_y
Cuts at: 0 569 2036 3273
Size: 569 1467 1237
Fragments arranged by size:
1467 1237 569
BtgI C'CryG_G
Cuts at: 0 79 356 758 1574 1586 1781 2032 2411
Size: 79 277 402 816 12 195 251 379
Cuts at: 2411 2540 2915 3006 3273
Size: 129 375 91 267
Fragments arranged by size:
816 402 379 375 277 267 251 195
129 91 79 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 738 2742 3273
Size: 28 710 2004 531
Fragments arranged by size:
2004 710 531 28
BtsI GCAGTG_nn'
Cuts at: 0 1738 1786 2446 3273
Size: 1738 48 660 827
Fragments arranged by size:
1738 827 660 48
DraI TTT'AAA
Cuts at: 0 1358 3273
Size: 1358 1915
DraIII CAC_nnn'GTG
Cuts at: 0 1371 3273
Size: 1371 1902
EaeI y'GGCC_r
Cuts at: 0 575 1235 2029 2078 2606 3080 3273
Size: 575 660 794 49 528 474 193
Fragments arranged by size:
794 660 575 528 474 193 49
EarI CTCTTCn'nnn_
Cuts at: 0 328 427 650 1432 1633 1933 3273
Size: 328 99 223 782 201 300 1340
Fragments arranged by size:
1340 782 328 300 223 201 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 3273
Size: 80 3193
EcoICRI GAG'CTC
Cuts at: 0 951 3037 3273
Size: 951 2086 236
Fragments arranged by size:
2086 951 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1148 1274 1315 1413 1739 2302 2425
Size: 631 517 126 41 98 326 563 123
Cuts at: 2425 2587 2638 3232 3273
Size: 162 51 594 41
Fragments arranged by size:
631 594 563 517 326 162 126 123
98 51 41 41
EcoO109I rG'GnC_Cy
Cuts at: 0 795 796 1260 1347 1466 3207 3245 3273
Size: 795 1 464 87 119 1741 38 28
Fragments arranged by size:
1741 795 464 119 87 38 28 1
EcoRV GAT'ATC
Cuts at: 0 1137 2246 3057 3273
Size: 1137 1109 811 216
Fragments arranged by size:
1137 1109 811 216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2742 2988 3237 3273
Size: 2742 246 249 36
Fragments arranged by size:
2742 249 246 36
FspI TGC'GCA
Cuts at: 0 2192 3273
Size: 2192 1081
HaeII r_GCGC'y
Cuts at: 0 24 1313 2858 3273
Size: 24 1289 1545 415
Fragments arranged by size:
1545 1289 415 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 394 406 426 458 490 917 949
Size: 374 20 12 20 32 32 427 32
Cuts at: 949 1121 1153 1201 1233 3021 3053 3170 3202
Size: 172 32 48 32 1788 32 117 32
Cuts at: 3202 3273
Size: 71
Fragments arranged by size:
1788 427 374 172 117 71 48 32
32 32 32 32 32 32 20 20
12
HincII GTy'rAC
Cuts at: 0 715 3273
Size: 715 2558
HindIII A'AGCT_T
Cuts at: 0 2151 3273
Size: 2151 1122
KasI G'GCGC_C
Cuts at: 0 20 3273
Size: 20 3253
MfeI C'AATT_G
Cuts at: 0 1679 3273
Size: 1679 1594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 677 2152 2732 3273
Size: 271 406 1475 580 541
Fragments arranged by size:
1475 580 541 406 271
MscI TGG'CCA
Cuts at: 0 577 1237 2031 2608 3082 3273
Size: 577 660 794 577 474 191
Fragments arranged by size:
794 660 577 577 474 191
MslI CAynn'nnrTG
Cuts at: 0 160 433 511 757 763 1147 1304 2071
Size: 160 273 78 246 6 384 157 767
Cuts at: 2071 2299 2833 2917 3235 3273
Size: 228 534 84 318 38
Fragments arranged by size:
767 534 384 318 273 246 228 160
157 84 78 38 6
MspA1I CmG'CkG
Cuts at: 0 81 2228 3273
Size: 81 2147 1045
Fragments arranged by size:
2147 1045 81
NaeI GCC'GGC
Cuts at: 0 746 3273
Size: 746 2527
NarI GG'CG_CC
Cuts at: 0 21 3273
Size: 21 3252
NcoI C'CATG_G
Cuts at: 0 356 758 1586 2411 2540 2915 3006 3273
Size: 356 402 828 825 129 375 91 267
Fragments arranged by size:
828 825 402 375 356 267 129 91
NgoMIV G'CCGG_C
Cuts at: 0 744 3273
Size: 744 2529
NsiI A_TGCA'T
Cuts at: 0 994 3273
Size: 994 2279
NspI r_CATG'y
Cuts at: 0 465 942 1095 1146 2094 2904 3273
Size: 465 477 153 51 948 810 369
Fragments arranged by size:
948 810 477 465 369 153 51
PasI CC'CwG_GG
Cuts at: 0 398 3273
Size: 398 2875
PciI A'CATG_T
Cuts at: 0 1091 2090 3273
Size: 1091 999 1183
Fragments arranged by size:
1183 1091 999
PflMI CCAn_nnn'nTGG
Cuts at: 0 2352 3273
Size: 2352 921
PfoI T'CCnGG_A
Cuts at: 0 3107 3273
Size: 3107 166
PmlI CAC'GTG
Cuts at: 0 2589 3167 3273
Size: 2589 578 106
Fragments arranged by size:
2589 578 106
PpuMI rG'GwC_Cy
Cuts at: 0 1260 1347 3207 3245 3273
Size: 1260 87 1860 38 28
Fragments arranged by size:
1860 1260 87 38 28
PsiI TTA'TAA
Cuts at: 0 1316 3273
Size: 1316 1957
PspOMI G'GGCC_C
Cuts at: 0 795 3273
Size: 795 2478
PstI C_TGCA'G
Cuts at: 0 794 1196 3152 3273
Size: 794 402 1956 121
Fragments arranged by size:
1956 794 402 121
PvuII CAG'CTG
Cuts at: 0 2228 3273
Size: 2228 1045
RsrII CG'GwC_CG
Cuts at: 0 1473 3273
Size: 1473 1800
SacI G_AGCT'C
Cuts at: 0 953 3039 3273
Size: 953 2086 234
Fragments arranged by size:
2086 953 234
SacII CC_GC'GG
Cuts at: 0 82 3273
Size: 82 3191
SanDI GG'GwC_CC
Cuts at: 0 1260 1347 3273
Size: 1260 87 1926
Fragments arranged by size:
1926 1260 87
SapI GCTCTTCn'nnn_
Cuts at: 0 650 3273
Size: 650 2623
ScaI AGT'ACT
Cuts at: 0 1760 3273
Size: 1760 1513
SexAI A'CCwGG_T
Cuts at: 0 404 557 1367 3273
Size: 404 153 810 1906
Fragments arranged by size:
1906 810 404 153
SfcI C'TryA_G
Cuts at: 0 790 1192 2223 2361 2481 3148 3273
Size: 790 402 1031 138 120 667 125
Fragments arranged by size:
1031 790 667 402 138 125 120
SfoI GGC'GCC
Cuts at: 0 22 3273
Size: 22 3251
SmaI CCC'GGG
Cuts at: 0 88 923 3273
Size: 88 835 2350
Fragments arranged by size:
2350 835 88
SmlI C'TyrA_G
Cuts at: 0 1617 1711 3273
Size: 1617 94 1562
Fragments arranged by size:
1617 1562 94
SpeI A'CTAG_T
Cuts at: 0 930 3273
Size: 930 2343
SspI AAT'ATT
Cuts at: 0 2476 3273
Size: 2476 797
StyI C'CwwG_G
Cuts at: 0 356 758 800 1232 1397 1586 2306 2411
Size: 356 402 42 432 165 189 720 105
Cuts at: 2411 2540 2915 3006 3273
Size: 129 375 91 267
Fragments arranged by size:
720 432 402 375 356 267 189 165
129 105 91 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1461 2353 3273
Size: 1461 892 920
Fragments arranged by size:
1461 920 892
TatI w'GTAC_w
Cuts at: 0 440 1758 2897 3273
Size: 440 1318 1139 376
Fragments arranged by size:
1318 1139 440 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 410 3273
Size: 378 32 2863
Fragments arranged by size:
2863 378 32
Tth111I GACn'n_nGTC
Cuts at: 0 2653 3273
Size: 2653 620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1249 1447 3273
Size: 1249 198 1826
Fragments arranged by size:
1826 1249 198
XmaI C'CCGG_G
Cuts at: 0 86 921 3273
Size: 86 835 2352
Fragments arranged by size:
2352 835 86
XmnI GAAnn'nnTTC
Cuts at: 0 1724 2509 2735 3273
Size: 1724 785 226 538
Fragments arranged by size:
1724 785 538 226
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PsiI PspOMI PstI PvuII RsrII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI
SspI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AloI AscI
AsiSI AvrII BamHI BbvCI BcgI BglII BmrI BmtI
BplI Bpu10I BsiEI BsiWI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DrdI EagI EcoNI EcoRI FseI FspAI
HpaI KpnI MluI NdeI NheI NotI NruI PacI
PmeI PpiI PshAI PspXI PsrI PvuI SalI SbfI
SfiI SgfI SgrAI SnaBI SphI SrfI StuI SwaI
XbaI XhoI ZraI