Restriction map for
pF1KE2583
(Linear) (Six-base) MAPSORT of: KIEE2583.seq Check: 6967 from: 1 to: 4926
KIEE2583.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2305 4926
Size: 2305 2621
AccI GT'mk_AC
Cuts at: 0 1538 4926
Size: 1538 3388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2383 2785 4184 4673 4926
Size: 2383 402 1399 489 253
Fragments arranged by size:
2383 1399 489 402 253
AfeI AGC'GCT
Cuts at: 0 3915 4926
Size: 3915 1011
AhdI GACnn_n'nnGTC
Cuts at: 0 981 4926
Size: 981 3945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2323 4926
Size: 2323 2603
AlwNI CAG_nnn'CTG
Cuts at: 0 2310 2506 4918 4926
Size: 2310 196 2412 8
Fragments arranged by size:
2412 2310 196 8
ApaI G_GGCC'C
Cuts at: 0 889 1304 4926
Size: 889 415 3622
Fragments arranged by size:
3622 889 415
ApoI r'AATT_y
Cuts at: 0 295 2410 2581 4926
Size: 295 2115 171 2345
Fragments arranged by size:
2345 2115 295 171
AseI AT'TA_AT
Cuts at: 0 3194 4926
Size: 3194 1732
AvaI C'yCGr_G
Cuts at: 0 3857 4445 4926
Size: 3857 588 481
Fragments arranged by size:
3857 588 481
AvrII C'CTAG_G
Cuts at: 0 4786 4926
Size: 4786 140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1205 1238 2922 2955 4926
Size: 1205 33 1684 33 1971
Fragments arranged by size:
1971 1684 1205 33 33
BanI G'GyrC_C
Cuts at: 0 760 778 1329 3259 4926
Size: 760 18 551 1930 1667
Fragments arranged by size:
1930 1667 760 551 18
BanII G_rGCy'C
Cuts at: 0 7 22 34 889 1304 1721 4926
Size: 7 15 12 855 415 417 3205
Fragments arranged by size:
3205 855 417 415 15 12 7
BbeI G_GCGC'C
Cuts at: 0 782 3263 4926
Size: 782 2481 1663
Fragments arranged by size:
2481 1663 782
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 2340 4078 4926
Size: 656 1684 1738 848
Fragments arranged by size:
1738 1684 848 656
BciVI GTATCCnnnnn_n'
Cuts at: 0 351 1955 3262 3966 4926
Size: 351 1604 1307 704 960
Fragments arranged by size:
1604 1307 960 704 351
BclI T'GATC_A
Cuts at: 0 488 3449 4926
Size: 488 2961 1477
Fragments arranged by size:
2961 1477 488
BglI GCCn_nnn'nGGC
Cuts at: 0 1308 2047 4926
Size: 1308 739 2879
Fragments arranged by size:
2879 1308 739
BglII A'GATC_T
Cuts at: 0 1286 2573 2823 4822 4926
Size: 1286 1287 250 1999 104
Fragments arranged by size:
1999 1287 1286 250 104
BlpI GC'TnA_GC
Cuts at: 0 1240 3770 4604 4926
Size: 1240 2530 834 322
Fragments arranged by size:
2530 1240 834 322
Bme1580I G_kGCm'C
Cuts at: 0 860 889 1304 4105 4926
Size: 860 29 415 2801 821
Fragments arranged by size:
2801 860 821 415 29
BmgBI CAC'GTC
Cuts at: 0 967 3989 4050 4926
Size: 967 3022 61 876
Fragments arranged by size:
3022 967 876 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 853 1494 1636 4108 4926
Size: 853 641 142 2472 818
Fragments arranged by size:
2472 853 818 641 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2770 3114 4926
Size: 2770 344 1812
Fragments arranged by size:
2770 1812 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1214 1624 1747 1841 2253 2677 3257
Size: 421 793 410 123 94 412 424 580
Cuts at: 3257 4926
Size: 1669
Fragments arranged by size:
1669 793 580 424 421 412 410 123
94
Bpu10I CC'TnA_GC
Cuts at: 0 3556 4926
Size: 3556 1370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 2496 4926
Size: 10 2486 2430
Fragments arranged by size:
2486 2430 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 4926
Size: 874 4052
BsaAI yAC'GTr
Cuts at: 0 558 2982 4926
Size: 558 2424 1944
Fragments arranged by size:
2424 1944 558
BsaBI GATnn'nnATC
Cuts at: 0 2707 3004 4752 4926
Size: 2707 297 1748 174
Fragments arranged by size:
2707 1748 297 174
BsaHI Gr'CG_yC
Cuts at: 0 779 3260 4926
Size: 779 2481 1666
Fragments arranged by size:
2481 1666 779
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 568 598 2068 2098 4926
Size: 568 30 1470 30 2828
Fragments arranged by size:
2828 1470 568 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 573 1621 1757 2542 3103 3241 3580 3607
Size: 573 1048 136 785 561 138 339 27
Cuts at: 3607 4926
Size: 1319
Fragments arranged by size:
1319 1048 785 573 561 339 138 136
27
BseYI C'CCAG_C
Cuts at: 0 190 4926
Size: 190 4736
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3625 4926
Size: 3625 1301
BsiEI CG_ry'CG
Cuts at: 0 3495 4926
Size: 3495 1431
BsiHKAI G_wGCw'C
Cuts at: 0 7 34 722 2540 3047 3614 4926
Size: 7 27 688 1818 507 567 1312
Fragments arranged by size:
1818 1312 688 567 507 27 7
BsmI GAATG_Cn'
Cuts at: 0 1497 3807 4926
Size: 1497 2310 1119
Fragments arranged by size:
2310 1497 1119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3490 4926
Size: 3490 1436
Bsp1286I G_dGCh'C
Cuts at: 0 7 22 34 722 860 889 1304 1721
Size: 7 15 12 688 138 29 415 417
Cuts at: 1721 2540 3047 3614 4105 4926
Size: 819 507 567 491 821
Fragments arranged by size:
821 819 688 567 507 491 417 415
138 29 15 12 7
BspHI T'CATG_A
Cuts at: 0 2846 3047 3420 4926
Size: 2846 201 373 1506
Fragments arranged by size:
2846 1506 373 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 926 2254 2305 2648 3332 4926
Size: 926 1328 51 343 684 1594
Fragments arranged by size:
1594 1328 926 684 343 51
BsrDI GCAATG_nn'
Cuts at: 0 2285 4534 4926
Size: 2285 2249 392
Fragments arranged by size:
2285 2249 392
BssSI C'ACGA_G
Cuts at: 0 862 871 1720 1954 2077 2827 4926
Size: 862 9 849 234 123 750 2099
Fragments arranged by size:
2099 862 849 750 234 123 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 855 1509 3603 4926
Size: 855 654 2094 1323
Fragments arranged by size:
2094 1323 855 654
BstXI CCAn_nnnn'nTGG
Cuts at: 0 909 942 2043 4926
Size: 909 33 1101 2883
Fragments arranged by size:
2883 1101 909 33
BstYI r'GATC_y
Cuts at: 0 518 1286 2573 2750 2823 4822 4926
Size: 518 768 1287 177 73 1999 104
Fragments arranged by size:
1999 1287 768 518 177 104 73
Bsu36I CC'TnA_GG
Cuts at: 0 1033 4926
Size: 1033 3893
BtgI C'CryG_G
Cuts at: 0 625 1376 2069 2261 3185 4926
Size: 625 751 693 192 924 1741
Fragments arranged by size:
1741 924 751 693 625 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1522 4926
Size: 1522 3404
BtsI GCAGTG_nn'
Cuts at: 0 364 1979 2006 3604 4601 4926
Size: 364 1615 27 1598 997 325
Fragments arranged by size:
1615 1598 997 364 325 27
ClaI AT'CG_AT
Cuts at: 0 3936 4926
Size: 3936 990
DraI TTT'AAA
Cuts at: 0 4259 4926
Size: 4259 667
DrdI GACnn_nn'nnGTC
Cuts at: 0 3430 4926
Size: 3430 1496
EaeI y'GGCC_r
Cuts at: 0 144 1309 1379 2033 2145 2258 2683 2831
Size: 144 1165 70 654 112 113 425 148
Cuts at: 2831 2895 3492 4926
Size: 64 597 1434
Fragments arranged by size:
1434 1165 654 597 425 148 144 113
112 70 64
EagI C'GGCC_G
Cuts at: 0 3492 4926
Size: 3492 1434
EarI CTCTTCn'nnn_
Cuts at: 0 197 920 1392 1892 2018 2471 3556 3950
Size: 197 723 472 500 126 453 1085 394
Cuts at: 3950 4034 4160 4926
Size: 84 126 766
Fragments arranged by size:
1085 766 723 500 472 453 394 197
126 126 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 323 1028 1673 4926
Size: 323 705 645 3253
Fragments arranged by size:
3253 705 645 323
EcoICRI GAG'CTC
Cuts at: 0 5 32 4926
Size: 5 27 4894
Fragments arranged by size:
4894 27 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1214 1624 1747 1841 2253 2383 2677
Size: 421 793 410 123 94 412 130 294
Cuts at: 2677 2785 3257 4184 4673 4926
Size: 108 472 927 489 253
Fragments arranged by size:
927 793 489 472 421 412 410 294
253 130 123 108 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 490 3844 4926
Size: 490 3354 1082
Fragments arranged by size:
3354 1082 490
EcoO109I rG'GnC_Cy
Cuts at: 0 512 885 886 1301 2040 4926
Size: 512 373 1 415 739 2886
Fragments arranged by size:
2886 739 512 415 373 1
EcoRI G'AATT_C
Cuts at: 0 295 2581 4926
Size: 295 2286 2345
Fragments arranged by size:
2345 2286 295
EcoRV GAT'ATC
Cuts at: 0 2919 3400 3940 4926
Size: 2919 481 540 986
Fragments arranged by size:
2919 986 540 481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2815 3743 4926
Size: 2815 928 1183
Fragments arranged by size:
2815 1183 928
FspI TGC'GCA
Cuts at: 0 992 4926
Size: 992 3934
HaeII r_GCGC'y
Cuts at: 0 782 3263 3917 4926
Size: 782 2481 654 1009
Fragments arranged by size:
2481 1009 782 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 568 600 967 999 2095 2127
Size: 226 32 310 32 367 32 1096 32
Cuts at: 2127 2416 2448 2494 2526 3533 3565 3625 3657
Size: 289 32 46 32 1007 32 60 32
Cuts at: 3657 3961 3993 4926
Size: 304 32 933
Fragments arranged by size:
1096 1007 933 367 310 304 289 226
60 46 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1539 2961 3756 4473 4926
Size: 1539 1422 795 717 453
Fragments arranged by size:
1539 1422 795 717 453
HindIII A'AGCT_T
Cuts at: 0 4431 4904 4926
Size: 4431 473 22
Fragments arranged by size:
4431 473 22
HpaI GTT'AAC
Cuts at: 0 4473 4926
Size: 4473 453
KasI G'GCGC_C
Cuts at: 0 778 3259 4926
Size: 778 2481 1667
Fragments arranged by size:
2481 1667 778
MfeI C'AATT_G
Cuts at: 0 4510 4926
Size: 4510 416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 719 3398 3683 4565 4926
Size: 639 80 2679 285 882 361
Fragments arranged by size:
2679 882 639 361 285 80
MscI TGG'CCA
Cuts at: 0 146 1381 2035 2833 4926
Size: 146 1235 654 798 2093
Fragments arranged by size:
2093 1235 798 654 146
MslI CAynn'nnrTG
Cuts at: 0 940 1183 1912 2665 2845 3478 4926
Size: 940 243 729 753 180 633 1448
Fragments arranged by size:
1448 940 753 729 633 243 180
MspA1I CmG'CkG
Cuts at: 0 396 962 1098 1239 1754 3405 3491 3497
Size: 396 566 136 141 515 1651 86 6
Cuts at: 3497 4926
Size: 1429
Fragments arranged by size:
1651 1429 566 515 396 141 136 86
6
NarI GG'CG_CC
Cuts at: 0 779 3260 4926
Size: 779 2481 1666
Fragments arranged by size:
2481 1666 779
NcoI C'CATG_G
Cuts at: 0 625 1376 2069 3185 4926
Size: 625 751 693 1116 1741
Fragments arranged by size:
1741 1116 751 693 625
NdeI CA'TA_TG
Cuts at: 0 2421 4926
Size: 2421 2505
NotI GC'GGCC_GC
Cuts at: 0 3492 4926
Size: 3492 1434
NspI r_CATG'y
Cuts at: 0 1578 3015 3132 4382 4834 4926
Size: 1578 1437 117 1250 452 92
Fragments arranged by size:
1578 1437 1250 452 117 92
PasI CC'CwG_GG
Cuts at: 0 765 1317 4926
Size: 765 552 3609
Fragments arranged by size:
3609 765 552
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 1995 2121 4189 4926
Size: 378 1617 126 2068 737
Fragments arranged by size:
2068 1617 737 378 126
PfoI T'CCnGG_A
Cuts at: 0 2693 2942 3617 4926
Size: 2693 249 675 1309
Fragments arranged by size:
2693 1309 675 249
PshAI GACnn'nnGTC
Cuts at: 0 4003 4926
Size: 4003 923
PsiI TTA'TAA
Cuts at: 0 1076 1111 1616 4926
Size: 1076 35 505 3310
Fragments arranged by size:
3310 1076 505 35
PspOMI G'GGCC_C
Cuts at: 0 885 1300 4926
Size: 885 415 3626
Fragments arranged by size:
3626 885 415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2866 2898 4926
Size: 2866 32 2028
Fragments arranged by size:
2866 2028 32
PstI C_TGCA'G
Cuts at: 0 827 2302 3502 3749 4596 4926
Size: 827 1475 1200 247 847 330
Fragments arranged by size:
1475 1200 847 827 330 247
PvuII CAG'CTG
Cuts at: 0 396 1098 1239 3405 4926
Size: 396 702 141 2166 1521
Fragments arranged by size:
2166 1521 702 396 141
SacI G_AGCT'C
Cuts at: 0 7 34 4926
Size: 7 27 4892
Fragments arranged by size:
4892 27 7
SalI G'TCGA_C
Cuts at: 0 1537 4926
Size: 1537 3389
SapI GCTCTTCn'nnn_
Cuts at: 0 920 3556 4926
Size: 920 2636 1370
Fragments arranged by size:
2636 1370 920
ScaI AGT'ACT
Cuts at: 0 1000 2809 4926
Size: 1000 1809 2117
Fragments arranged by size:
2117 1809 1000
SexAI A'CCwGG_T
Cuts at: 0 653 3377 4926
Size: 653 2724 1549
Fragments arranged by size:
2724 1549 653
SfcI C'TryA_G
Cuts at: 0 611 823 2298 2869 3498 3745 3924 4592
Size: 611 212 1475 571 629 247 179 668
Cuts at: 4592 4926
Size: 334
Fragments arranged by size:
1475 668 629 611 571 334 247 212
179
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1308 2047 4926
Size: 1308 739 2879
Fragments arranged by size:
2879 1308 739
SfoI GGC'GCC
Cuts at: 0 780 3261 4926
Size: 780 2481 1665
Fragments arranged by size:
2481 1665 780
SmaI CCC'GGG
Cuts at: 0 3859 4447 4926
Size: 3859 588 479
Fragments arranged by size:
3859 588 479
SmlI C'TyrA_G
Cuts at: 0 25 2511 4926
Size: 25 2486 2415
Fragments arranged by size:
2486 2415 25
StuI AGG'CCT
Cuts at: 0 434 485 1815 4926
Size: 434 51 1330 3111
Fragments arranged by size:
3111 1330 434 51
StyI C'CwwG_G
Cuts at: 0 625 1376 1388 2036 2069 2447 3185 4786
Size: 625 751 12 648 33 378 738 1601
Cuts at: 4786 4926
Size: 140
Fragments arranged by size:
1601 751 738 648 625 378 140 33
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 274 751 1389 1476 2332 4926
Size: 204 70 477 638 87 856 2594
Fragments arranged by size:
2594 856 638 477 204 87 70
TatI w'GTAC_w
Cuts at: 0 998 2807 3017 4487 4926
Size: 998 1809 210 1470 439
Fragments arranged by size:
1809 1470 998 439 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2068 2100 4554 4586 4926
Size: 2068 32 2454 32 340
Fragments arranged by size:
2454 2068 340 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 690 1628 4926
Size: 690 938 3298
Fragments arranged by size:
3298 938 690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1599 2043 2403 3312 4926
Size: 1599 444 360 909 1614
Fragments arranged by size:
1614 1599 909 444 360
XmaI C'CCGG_G
Cuts at: 0 3857 4445 4926
Size: 3857 588 481
Fragments arranged by size:
3857 588 481
XmnI GAAnn'nnTTC
Cuts at: 0 1158 3550 4658 4926
Size: 1158 2392 1108 268
Fragments arranged by size:
2392 1158 1108 268
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
ApoI AseI AvaI AvrII BaeI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NotI NspI PasI PflMI PfoI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SalI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaLI AscI AsiSI BamHI BbvCI BcgI BfrBI BmtI
BsaWI BsiWI BspEI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I CspCI DraIII FseI FspAI KpnI MluI
NaeI NgoMIV NheI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PspXI PvuI RsrII SacII SanDI
SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI ZraI