Restriction map for
pF1KE2586
(Linear) (Six-base) MAPSORT of: KIEE2586.seq Check: 7187 from: 1 to: 2373
KIEE2586.orf
With 182 enzymes: SgfI *
August 7, 2015 15:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1908 2373
Size: 1908 465
AccI GT'mk_AC
Cuts at: 0 29 2373
Size: 29 2344
Acc65I G'GTAC_C
Cuts at: 0 376 2373
Size: 376 1997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1620 1863 2373
Size: 105 1515 243 510
Fragments arranged by size:
1515 510 243 105
AflIII A'CryG_T
Cuts at: 0 512 2373
Size: 512 1861
AleI CACnn'nnGTG
Cuts at: 0 964 1861 2373
Size: 964 897 512
Fragments arranged by size:
964 897 512
AlwNI CAG_nnn'CTG
Cuts at: 0 645 1791 2312 2373
Size: 645 1146 521 61
Fragments arranged by size:
1146 645 521 61
ApaI G_GGCC'C
Cuts at: 0 1569 2373
Size: 1569 804
ApoI r'AATT_y
Cuts at: 0 439 1148 2373
Size: 439 709 1225
Fragments arranged by size:
1225 709 439
BamHI G'GATC_C
Cuts at: 0 244 2373
Size: 244 2129
BanI G'GyrC_C
Cuts at: 0 369 376 787 1855 2373
Size: 369 7 411 1068 518
Fragments arranged by size:
1068 518 411 369 7
BanII G_rGCy'C
Cuts at: 0 815 1308 1569 1946 2062 2259 2276 2373
Size: 815 493 261 377 116 197 17 97
Fragments arranged by size:
815 493 377 261 197 116 97 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 1473 2082 2373
Size: 441 1032 609 291
Fragments arranged by size:
1032 609 441 291
BbvCI CC'TCA_GC
Cuts at: 0 132 1426 1928 2373
Size: 132 1294 502 445
Fragments arranged by size:
1294 502 445 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 854 2373
Size: 820 34 1519
Fragments arranged by size:
1519 820 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1289 2373
Size: 1289 1084
BclI T'GATC_A
Cuts at: 0 1247 2373
Size: 1247 1126
BglI GCCn_nnn'nGGC
Cuts at: 0 496 2138 2373
Size: 496 1642 235
Fragments arranged by size:
1642 496 235
BglII A'GATC_T
Cuts at: 0 1631 2373
Size: 1631 742
BlpI GC'TnA_GC
Cuts at: 0 2056 2270 2373
Size: 2056 214 103
Fragments arranged by size:
2056 214 103
Bme1580I G_kGCm'C
Cuts at: 0 1569 2373
Size: 1569 804
BmgBI CAC'GTC
Cuts at: 0 1086 2175 2229 2373
Size: 1086 1089 54 144
Fragments arranged by size:
1089 1086 144 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 88 1940 2322 2373
Size: 88 1852 382 51
Fragments arranged by size:
1852 382 88 51
BmtI G_CTAG'C
Cuts at: 0 529 2373
Size: 529 1844
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1769 2373
Size: 1769 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 2243 2373
Size: 1220 1023 130
Fragments arranged by size:
1220 1023 130
Bpu10I CC'TnA_GC
Cuts at: 0 132 1426 1928 2373
Size: 132 1294 502 445
Fragments arranged by size:
1294 502 445 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1309 1321 1690 2373
Size: 643 666 12 369 683
Fragments arranged by size:
683 666 643 369 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 984 1757 2350 2373
Size: 984 773 593 23
Fragments arranged by size:
984 773 593 23
BsaAI yAC'GTr
Cuts at: 0 24 513 1598 2373
Size: 24 489 1085 775
Fragments arranged by size:
1085 775 489 24
BsaBI GATnn'nnATC
Cuts at: 0 1588 2035 2373
Size: 1588 447 338
Fragments arranged by size:
1588 447 338
BsaHI Gr'CG_yC
Cuts at: 0 2141 2373
Size: 2141 232
BsaWI w'CCGG_w
Cuts at: 0 83 2373
Size: 83 2290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 306 1404 1761 1884 2308 2373
Size: 306 1098 357 123 424 65
Fragments arranged by size:
1098 424 357 306 123 65
BseYI C'CCAG_C
Cuts at: 0 2182 2373
Size: 2182 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 2373
Size: 493 1880
BsiEI CG_ry'CG
Cuts at: 0 629 2373
Size: 629 1744
BsiHKAI G_wGCw'C
Cuts at: 0 176 659 815 2062 2276 2373
Size: 176 483 156 1247 214 97
Fragments arranged by size:
1247 483 214 176 156 97
BsmI GAATG_Cn'
Cuts at: 0 1815 2170 2373
Size: 1815 355 203
Fragments arranged by size:
1815 355 203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2219 2373
Size: 2219 154
Bsp1286I G_dGCh'C
Cuts at: 0 176 659 815 1308 1569 1946 2062 2259
Size: 176 483 156 493 261 377 116 197
Cuts at: 2259 2276 2373
Size: 17 97
Fragments arranged by size:
493 483 377 261 197 176 156 116
97 17
BspHI T'CATG_A
Cuts at: 0 620 1760 2373
Size: 620 1140 613
Fragments arranged by size:
1140 620 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 1908 2373
Size: 230 1678 465
Fragments arranged by size:
1678 465 230
BsrDI GCAATG_nn'
Cuts at: 0 120 2373
Size: 120 2253
BsrFI r'CCGG_y
Cuts at: 0 631 2373
Size: 631 1742
BsrGI T'GTAC_A
Cuts at: 0 2117 2373
Size: 2117 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1791 2373
Size: 1791 582
BstBI TT'CG_AA
Cuts at: 0 1093 1497 2373
Size: 1093 404 876
Fragments arranged by size:
1093 876 404
BstYI r'GATC_y
Cuts at: 0 244 641 1268 1631 2373
Size: 244 397 627 363 742
Fragments arranged by size:
742 627 397 363 244
BtgI C'CryG_G
Cuts at: 0 491 2133 2373
Size: 491 1642 240
Fragments arranged by size:
1642 491 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 741 1635 2373
Size: 741 894 738
Fragments arranged by size:
894 741 738
BtsI GCAGTG_nn'
Cuts at: 0 1454 2373
Size: 1454 919
DraI TTT'AAA
Cuts at: 0 1146 2373
Size: 1146 1227
EaeI y'GGCC_r
Cuts at: 0 284 584 626 1064 1217 1958 2048 2373
Size: 284 300 42 438 153 741 90 325
Fragments arranged by size:
741 438 325 300 284 153 90 42
EagI C'GGCC_G
Cuts at: 0 626 2373
Size: 626 1747
EarI CTCTTCn'nnn_
Cuts at: 0 61 1366 2373
Size: 61 1305 1007
Fragments arranged by size:
1305 1007 61
EcoICRI GAG'CTC
Cuts at: 0 813 2060 2274 2373
Size: 813 1247 214 99
Fragments arranged by size:
1247 813 214 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1220 1620 1863 2243 2373
Size: 105 1115 400 243 380 130
Fragments arranged by size:
1115 400 380 243 130 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1560 2373
Size: 1560 813
EcoO109I rG'GnC_Cy
Cuts at: 0 14 255 1565 1932 2043 2373
Size: 14 241 1310 367 111 330
Fragments arranged by size:
1310 367 330 241 111 14
EcoRV GAT'ATC
Cuts at: 0 1575 2373
Size: 1575 798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 684 858 2109 2193 2373
Size: 684 174 1251 84 180
Fragments arranged by size:
1251 684 180 174 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 665 697 1095 1127 1295 1327 1879 1911
Size: 665 32 398 32 168 32 552 32
Cuts at: 1911 2373
Size: 462
Fragments arranged by size:
665 552 462 398 168 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 380 2373
Size: 380 1993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 2373
Size: 969 1404
MscI TGG'CCA
Cuts at: 0 286 586 1066 1219 1960 2050 2373
Size: 286 300 480 153 741 90 323
Fragments arranged by size:
741 480 323 300 286 153 90
MslI CAynn'nnrTG
Cuts at: 0 400 550 964 1540 1861 2373
Size: 400 150 414 576 321 512
Fragments arranged by size:
576 512 414 400 321 150
MspA1I CmG'CkG
Cuts at: 0 239 423 785 1927 2373
Size: 239 184 362 1142 446
Fragments arranged by size:
1142 446 362 239 184
NaeI GCC'GGC
Cuts at: 0 633 2373
Size: 633 1740
NcoI C'CATG_G
Cuts at: 0 491 2133 2373
Size: 491 1642 240
Fragments arranged by size:
1642 491 240
NgoMIV G'CCGG_C
Cuts at: 0 631 2373
Size: 631 1742
NheI G'CTAG_C
Cuts at: 0 525 2373
Size: 525 1848
NspI r_CATG'y
Cuts at: 0 831 927 1956 2346 2373
Size: 831 96 1029 390 27
Fragments arranged by size:
1029 831 390 96 27
PasI CC'CwG_GG
Cuts at: 0 700 2373
Size: 700 1673
PflMI CCAn_nnn'nTGG
Cuts at: 0 1524 2373
Size: 1524 849
PfoI T'CCnGG_A
Cuts at: 0 1997 2373
Size: 1997 376
PmlI CAC'GTG
Cuts at: 0 513 2373
Size: 513 1860
PpuMI rG'GwC_Cy
Cuts at: 0 255 2373
Size: 255 2118
PspOMI G'GGCC_C
Cuts at: 0 1565 2373
Size: 1565 808
PstI C_TGCA'G
Cuts at: 0 300 422 641 1123 1787 1796 2373
Size: 300 122 219 482 664 9 577
Fragments arranged by size:
664 577 482 300 219 122 9
PvuII CAG'CTG
Cuts at: 0 423 2373
Size: 423 1950
SacI G_AGCT'C
Cuts at: 0 815 2062 2276 2373
Size: 815 1247 214 97
Fragments arranged by size:
1247 815 214 97
SapI GCTCTTCn'nnn_
Cuts at: 0 1366 2373
Size: 1366 1007
SbfI CC_TGCA'GG
Cuts at: 0 1787 2373
Size: 1787 586
SfcI C'TryA_G
Cuts at: 0 296 418 637 854 1119 1783 1792 2373
Size: 296 122 219 217 265 664 9 581
Fragments arranged by size:
664 581 296 265 219 217 122 9
SmlI C'TyrA_G
Cuts at: 0 658 1324 1336 1705 2373
Size: 658 666 12 369 668
Fragments arranged by size:
668 666 658 369 12
StyI C'CwwG_G
Cuts at: 0 491 2133 2250 2373
Size: 491 1642 117 123
Fragments arranged by size:
1642 491 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1441 2373
Size: 1441 932
TatI w'GTAC_w
Cuts at: 0 662 2117 2373
Size: 662 1455 256
Fragments arranged by size:
1455 662 256
Tth111I GACn'n_nGTC
Cuts at: 0 2197 2373
Size: 2197 176
XbaI T'CTAG_A
Cuts at: 0 2160 2373
Size: 2160 213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 1260 1662 1953 2373
Size: 549 711 402 291 420
Fragments arranged by size:
711 549 420 402 291
XmnI GAAnn'nnTTC
Cuts at: 0 2089 2373
Size: 2089 284
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaI
ApoI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SapI SbfI SfcI SmlI StyI TaqII
TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BfrBI BsaXI BsiWI BspEI BsrBI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EciI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI NarI NdeI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XhoI XmaI ZraI