(Linear) (Six-base) MAPSORT of: KIEE2590.seq Check: 7567 from: 1 to: 2385 KIEE2590.orf With 182 enzymes: SgfI * September 30, 2015 09:49 .. Acc65I G'GTAC_C Cuts at: 0 1643 2327 2385 Size: 1643 684 58 Fragments arranged by size: 1643 684 58 AclI AA'CG_TT Cuts at: 0 814 2385 Size: 814 1571 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 849 1648 1795 1843 2004 2006 2385 Size: 499 350 799 147 48 161 2 379 Fragments arranged by size: 799 499 379 350 161 147 48 2 AflIII A'CryG_T Cuts at: 0 746 2385 Size: 746 1639 AhdI GACnn_n'nnGTC Cuts at: 0 2229 2385 Size: 2229 156 AlwNI CAG_nnn'CTG Cuts at: 0 1605 2173 2385 Size: 1605 568 212 Fragments arranged by size: 1605 568 212 ApoI r'AATT_y Cuts at: 0 1197 1278 2385 Size: 1197 81 1107 Fragments arranged by size: 1197 1107 81 AvaI C'yCGr_G Cuts at: 0 496 1658 2212 2385 Size: 496 1162 554 173 Fragments arranged by size: 1162 554 496 173 BamHI G'GATC_C Cuts at: 0 405 531 2385 Size: 405 126 1854 Fragments arranged by size: 1854 405 126 BanI G'GyrC_C Cuts at: 0 174 1635 1643 2327 2385 Size: 174 1461 8 684 58 Fragments arranged by size: 1461 684 174 58 8 BanII G_rGCy'C Cuts at: 0 680 760 2385 Size: 680 80 1625 Fragments arranged by size: 1625 680 80 BbeI G_GCGC'C Cuts at: 0 1639 2385 Size: 1639 746 BbsI GAAGACnn'nnnn_ Cuts at: 0 960 1019 1118 1956 2385 Size: 960 59 99 838 429 Fragments arranged by size: 960 838 429 99 59 BbvCI CC'TCA_GC Cuts at: 0 1811 2248 2385 Size: 1811 437 137 Fragments arranged by size: 1811 437 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1621 1655 1917 1951 2385 Size: 1621 34 262 34 434 Fragments arranged by size: 1621 434 262 34 34 BclI T'GATC_A Cuts at: 0 23 2385 Size: 23 2362 BglI GCCn_nnn'nGGC Cuts at: 0 55 2385 Size: 55 2330 Bme1580I G_kGCm'C Cuts at: 0 179 2385 Size: 179 2206 BmrI ACTGGGnnnn_n' Cuts at: 0 531 1346 1506 1830 2385 Size: 531 815 160 324 555 Fragments arranged by size: 815 555 531 324 160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1545 2385 Size: 1545 840 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1934 1982 2311 2385 Size: 1934 48 329 74 Fragments arranged by size: 1934 329 74 48 Bpu10I CC'TnA_GC Cuts at: 0 382 686 1811 2248 2323 2385 Size: 382 304 1125 437 75 62 Fragments arranged by size: 1125 437 382 304 75 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 2385 Size: 760 1625 BsaI GGTCTCn'nnnn_ Cuts at: 0 1400 2385 Size: 1400 985 BsaAI yAC'GTr Cuts at: 0 1746 2220 2385 Size: 1746 474 165 Fragments arranged by size: 1746 474 165 BsaHI Gr'CG_yC Cuts at: 0 1636 2385 Size: 1636 749 BsaWI w'CCGG_w Cuts at: 0 731 1478 2385 Size: 731 747 907 Fragments arranged by size: 907 747 731 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 237 267 2385 Size: 237 30 2118 Fragments arranged by size: 2118 237 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 258 261 881 915 1111 1302 1591 2232 Size: 258 3 620 34 196 191 289 641 Cuts at: 2232 2310 2385 Size: 78 75 Fragments arranged by size: 641 620 289 258 196 191 78 75 34 3 BseYI C'CCAG_C Cuts at: 0 36 58 190 413 1071 1372 2121 2385 Size: 36 22 132 223 658 301 749 264 Fragments arranged by size: 749 658 301 264 223 132 36 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 1004 2385 Size: 170 834 1381 Fragments arranged by size: 1381 834 170 BsiHKAI G_wGCw'C Cuts at: 0 316 2219 2385 Size: 316 1903 166 Fragments arranged by size: 1903 316 166 Bsp1286I G_dGCh'C Cuts at: 0 179 316 680 760 2219 2385 Size: 179 137 364 80 1459 166 Fragments arranged by size: 1459 364 179 166 137 80 BspEI T'CCGG_A Cuts at: 0 1478 2385 Size: 1478 907 BspHI T'CATG_A Cuts at: 0 1799 2093 2385 Size: 1799 294 292 Fragments arranged by size: 1799 294 292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 474 1269 2385 Size: 474 795 1116 Fragments arranged by size: 1116 795 474 BsrDI GCAATG_nn' Cuts at: 0 2309 2385 Size: 2309 76 BstYI r'GATC_y Cuts at: 0 405 531 1122 2036 2385 Size: 405 126 591 914 349 Fragments arranged by size: 914 591 405 349 126 BtsI GCAGTG_nn' Cuts at: 0 347 564 2385 Size: 347 217 1821 Fragments arranged by size: 1821 347 217 EarI CTCTTCn'nnn_ Cuts at: 0 1224 1423 1495 1696 2242 2385 Size: 1224 199 72 201 546 143 Fragments arranged by size: 1224 546 201 199 143 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1115 2245 2385 Size: 1115 1130 140 Fragments arranged by size: 1130 1115 140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 849 1648 1795 1843 1934 1982 2004 Size: 499 350 799 147 48 91 48 22 Cuts at: 2004 2006 2311 2385 Size: 2 305 74 Fragments arranged by size: 799 499 350 305 147 91 74 48 48 22 2 EcoO109I rG'GnC_Cy Cuts at: 0 360 722 1340 2385 Size: 360 362 618 1045 Fragments arranged by size: 1045 618 362 360 EcoRV GAT'ATC Cuts at: 0 149 2385 Size: 149 2236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 468 1209 2385 Size: 468 741 1176 Fragments arranged by size: 1176 741 468 HaeII r_GCGC'y Cuts at: 0 1605 1639 1980 2385 Size: 1605 34 341 405 Fragments arranged by size: 1605 405 341 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 133 165 454 486 577 609 2051 2083 Size: 133 32 289 32 91 32 1442 32 Cuts at: 2083 2215 2247 2356 2385 Size: 132 32 109 29 Fragments arranged by size: 1442 289 133 132 109 91 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 1611 2385 Size: 1611 774 KasI G'GCGC_C Cuts at: 0 1635 2385 Size: 1635 750 KpnI G_GTAC'C Cuts at: 0 1647 2331 2385 Size: 1647 684 54 Fragments arranged by size: 1647 684 54 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1296 1760 2385 Size: 1296 464 625 Fragments arranged by size: 1296 625 464 MspA1I CmG'CkG Cuts at: 0 40 2199 2385 Size: 40 2159 186 Fragments arranged by size: 2159 186 40 NarI GG'CG_CC Cuts at: 0 1636 2385 Size: 1636 749 NspI r_CATG'y Cuts at: 0 826 2385 Size: 826 1559 PasI CC'CwG_GG Cuts at: 0 725 2127 2385 Size: 725 1402 258 Fragments arranged by size: 1402 725 258 PflMI CCAn_nnn'nTGG Cuts at: 0 439 1518 1605 1862 2385 Size: 439 1079 87 257 523 Fragments arranged by size: 1079 523 439 257 87 PmlI CAC'GTG Cuts at: 0 2220 2385 Size: 2220 165 PpuMI rG'GwC_Cy Cuts at: 0 360 1340 2385 Size: 360 980 1045 Fragments arranged by size: 1045 980 360 PspXI vC'TCGA_Gb Cuts at: 0 496 2212 2385 Size: 496 1716 173 Fragments arranged by size: 1716 496 173 PstI C_TGCA'G Cuts at: 0 45 1025 2385 Size: 45 980 1360 Fragments arranged by size: 1360 980 45 PvuII CAG'CTG Cuts at: 0 40 2385 Size: 40 2345 SanDI GG'GwC_CC Cuts at: 0 1340 2385 Size: 1340 1045 SapI GCTCTTCn'nnn_ Cuts at: 0 1696 2385 Size: 1696 689 ScaI AGT'ACT Cuts at: 0 1699 2385 Size: 1699 686 SexAI A'CCwGG_T Cuts at: 0 2261 2385 Size: 2261 124 SfcI C'TryA_G Cuts at: 0 41 1021 2385 Size: 41 980 1364 Fragments arranged by size: 1364 980 41 SfoI GGC'GCC Cuts at: 0 1637 2385 Size: 1637 748 SmaI CCC'GGG Cuts at: 0 1660 2385 Size: 1660 725 SmlI C'TyrA_G Cuts at: 0 496 739 2212 2385 Size: 496 243 1473 173 Fragments arranged by size: 1473 496 243 173 StyI C'CwwG_G Cuts at: 0 1401 1856 2335 2385 Size: 1401 455 479 50 Fragments arranged by size: 1401 479 455 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 204 1774 2385 Size: 204 1570 611 Fragments arranged by size: 1570 611 204 TatI w'GTAC_w Cuts at: 0 1697 2385 Size: 1697 688 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 237 269 982 1014 2162 2194 2385 Size: 237 32 713 32 1148 32 191 Fragments arranged by size: 1148 713 237 191 32 32 32 XbaI T'CTAG_A Cuts at: 0 1119 2385 Size: 1119 1266 XhoI C'TCGA_G Cuts at: 0 496 2212 2385 Size: 496 1716 173 Fragments arranged by size: 1716 496 173 XmaI C'CCGG_G Cuts at: 0 1658 2385 Size: 1658 727 XmnI GAAnn'nnTTC Cuts at: 0 1115 2385 Size: 1115 1270 Enzymes that do cut: Acc65I AclI AcuI AflIII AhdI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BstYI BtsI EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MspA1I NarI NspI PasI PflMI PmlI PpuMI PspXI PstI PvuII SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XbaI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglII BlpI BmgBI BmtI BsaBI BsiEI BsiWI BsmI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XcmI ZraI