Restriction map for
pF1KE2597
(Linear) (Six-base) MAPSORT of: KIEE2597.seq Check: 3229 from: 1 to: 5817
KIEE2597.orf
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1254 5817
Size: 1254 4563
AccI GT'mk_AC
Cuts at: 0 1301 5529 5817
Size: 1301 4228 288
Fragments arranged by size:
4228 1301 288
Acc65I G'GTAC_C
Cuts at: 0 766 5817
Size: 766 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1163 1947 2219 2225 2711 4615 5032
Size: 1019 144 784 272 6 486 1904 417
Cuts at: 5032 5338 5446 5678 5817
Size: 306 108 232 139
Fragments arranged by size:
1904 1019 784 486 417 306 272 232
144 139 108 6
AfeI AGC'GCT
Cuts at: 0 325 3418 5817
Size: 325 3093 2399
Fragments arranged by size:
3093 2399 325
AflIII A'CryG_T
Cuts at: 0 1484 4218 5817
Size: 1484 2734 1599
Fragments arranged by size:
2734 1599 1484
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 3505 3537 5111 5143 5817
Size: 1609 32 1864 32 1574 32 674
Fragments arranged by size:
1864 1609 1574 674 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 604 1072 1347 1900 3030 3660 3816 4200
Size: 604 468 275 553 1130 630 156 384
Cuts at: 4200 5157 5454 5610 5817
Size: 957 297 156 207
Fragments arranged by size:
1130 957 630 604 553 468 384 297
275 207 156 156
ApoI r'AATT_y
Cuts at: 0 745 1019 1096 1971 2281 2575 4521 5695
Size: 745 274 77 875 310 294 1946 1174
Cuts at: 5695 5817
Size: 122
Fragments arranged by size:
1946 1174 875 745 310 294 274 122
77
AvaI C'yCGr_G
Cuts at: 0 3395 3431 3915 5817
Size: 3395 36 484 1902
Fragments arranged by size:
3395 1902 484 36
AvrII C'CTAG_G
Cuts at: 0 1213 5817
Size: 1213 4604
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 750 783 5817
Size: 750 33 5034
Fragments arranged by size:
5034 750 33
BamHI G'GATC_C
Cuts at: 0 755 5705 5817
Size: 755 4950 112
Fragments arranged by size:
4950 755 112
BanI G'GyrC_C
Cuts at: 0 766 1759 2056 5817
Size: 766 993 297 3761
Fragments arranged by size:
3761 993 766 297
BanII G_rGCy'C
Cuts at: 0 1577 2327 5129 5817
Size: 1577 750 2802 688
Fragments arranged by size:
2802 1577 750 688
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 559 1858 2308 4264 5243 5585 5817
Size: 222 337 1299 450 1956 979 342 232
Fragments arranged by size:
1956 1299 979 450 342 337 232 222
BbvCI CC'TCA_GC
Cuts at: 0 2089 2789 3483 4211 4922 5309 5770 5817
Size: 2089 700 694 728 711 387 461 47
Fragments arranged by size:
2089 728 711 700 694 461 387 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3685 3719 5094 5128 5817
Size: 3685 34 1375 34 689
Fragments arranged by size:
3685 1375 689 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4959 5817
Size: 4959 858
BclI T'GATC_A
Cuts at: 0 527 914 3818 5180 5817
Size: 527 387 2904 1362 637
Fragments arranged by size:
2904 1362 637 527 387
BfrBI ATG'CAT
Cuts at: 0 1080 5817
Size: 1080 4737
BglI GCCn_nnn'nGGC
Cuts at: 0 3397 3475 3577 5281 5305 5817
Size: 3397 78 102 1704 24 512
Fragments arranged by size:
3397 1704 512 102 78 24
BglII A'GATC_T
Cuts at: 0 894 1188 1406 5817
Size: 894 294 218 4411
Fragments arranged by size:
4411 894 294 218
BlpI GC'TnA_GC
Cuts at: 0 1127 2813 2981 4193 5411 5817
Size: 1127 1686 168 1212 1218 406
Fragments arranged by size:
1686 1218 1212 1127 406 168
Bme1580I G_kGCm'C
Cuts at: 0 971 3996 5817
Size: 971 3025 1821
Fragments arranged by size:
3025 1821 971
BmrI ACTGGGnnnn_n'
Cuts at: 0 614 2268 4113 5817
Size: 614 1654 1845 1704
Fragments arranged by size:
1845 1704 1654 614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2021 3408 3741 5817
Size: 2021 1387 333 2076
Fragments arranged by size:
2076 2021 1387 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 709 832 1447 1516 2662 2860 2992
Size: 218 491 123 615 69 1146 198 132
Cuts at: 2992 3385 3460 3565 3682 4189 4258 5221 5368
Size: 393 75 105 117 507 69 963 147
Cuts at: 5368 5476 5587 5740 5817
Size: 108 111 153 77
Fragments arranged by size:
1146 963 615 507 491 393 218 198
153 147 132 123 117 111 108 105
77 75 69 69
Bpu10I CC'TnA_GC
Cuts at: 0 2089 2789 3483 4211 4922 5309 5770 5817
Size: 2089 700 694 728 711 387 461 47
Fragments arranged by size:
2089 728 711 700 694 461 387 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2016 2845 2902 3182 4273 4720 4837 4945
Size: 2016 829 57 280 1091 447 117 108
Cuts at: 4945 5817
Size: 872
Fragments arranged by size:
2016 1091 872 829 447 280 117 108
57
BsaI GGTCTCn'nnnn_
Cuts at: 0 3176 4865 5817
Size: 3176 1689 952
Fragments arranged by size:
3176 1689 952
BsaBI GATnn'nnATC
Cuts at: 0 3691 5817
Size: 3691 2126
BsaWI w'CCGG_w
Cuts at: 0 532 3143 4994 5817
Size: 532 2611 1851 823
Fragments arranged by size:
2611 1851 823 532
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 451 481 502 532 1420 1450
Size: 191 30 230 30 21 30 888 30
Cuts at: 1450 1609 1639 2034 2064 3505 3535 5111 5141
Size: 159 30 395 30 1441 30 1576 30
Cuts at: 5141 5817
Size: 676
Fragments arranged by size:
1576 1441 888 676 395 230 191 159
30 30 30 30 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 720 1132 1519 1630 1855 2013 2347 2518
Size: 720 412 387 111 225 158 334 171
Cuts at: 2518 3064 3373 3382 3457 3562 3817 3988 4069
Size: 546 309 9 75 105 255 171 81
Cuts at: 4069 4072 4177 4186 4660 5083 5115 5326 5737
Size: 3 105 9 474 423 32 211 411
Cuts at: 5737 5817
Size: 80
Fragments arranged by size:
720 546 474 423 412 411 387 334
309 255 225 211 171 171 158 111
105 105 81 80 75 32 9 9
3
BseYI C'CCAG_C
Cuts at: 0 970 2357 4970 5817
Size: 970 1387 2613 847
Fragments arranged by size:
2613 1387 970 847
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1279 2406 2558 5143 5817
Size: 175 1104 1127 152 2585 674
Fragments arranged by size:
2585 1127 1104 674 175 152
BsiHKAI G_wGCw'C
Cuts at: 0 1287 1577 2005 4810 5129 5817
Size: 1287 290 428 2805 319 688
Fragments arranged by size:
2805 1287 688 428 319 290
BsmI GAATG_Cn'
Cuts at: 0 4239 4473 5817
Size: 4239 234 1344
Fragments arranged by size:
4239 1344 234
Bsp1286I G_dGCh'C
Cuts at: 0 971 1287 1577 2005 2327 3996 4810 5129
Size: 971 316 290 428 322 1669 814 319
Cuts at: 5129 5817
Size: 688
Fragments arranged by size:
1669 971 814 688 428 322 319 316
290
BspEI T'CCGG_A
Cuts at: 0 3143 4994 5817
Size: 3143 1851 823
Fragments arranged by size:
3143 1851 823
BspHI T'CATG_A
Cuts at: 0 2474 4295 5817
Size: 2474 1821 1522
Fragments arranged by size:
2474 1821 1522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1254 3047 3647 4043 5057 5396 5817
Size: 1254 1793 600 396 1014 339 421
Fragments arranged by size:
1793 1254 1014 600 421 396 339
BsrBI CCG'CTC
Cuts at: 0 1888 3275 3387 4436 5352 5817
Size: 1888 1387 112 1049 916 465
Fragments arranged by size:
1888 1387 1049 916 465 112
BsrDI GCAATG_nn'
Cuts at: 0 1236 1391 1775 1853 2975 4097 5265 5514
Size: 1236 155 384 78 1122 1122 1168 249
Cuts at: 5514 5744 5817
Size: 230 73
Fragments arranged by size:
1236 1168 1122 1122 384 249 230 155
78 73
BsrFI r'CCGG_y
Cuts at: 0 1780 3469 5817
Size: 1780 1689 2348
Fragments arranged by size:
2348 1780 1689
BsrGI T'GTAC_A
Cuts at: 0 308 1268 5817
Size: 308 960 4549
Fragments arranged by size:
4549 960 308
BssHII G'CGCG_C
Cuts at: 0 3844 5817
Size: 3844 1973
BssSI C'ACGA_G
Cuts at: 0 292 5713 5817
Size: 292 5421 104
Fragments arranged by size:
5421 292 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 595 1282 5817
Size: 595 687 4535
Fragments arranged by size:
4535 687 595
BstEII G'GTnAC_C
Cuts at: 0 1326 5817
Size: 1326 4491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4914 5817
Size: 4914 903
BstYI r'GATC_y
Cuts at: 0 755 894 1188 1406 3118 3150 4364 4682
Size: 755 139 294 218 1712 32 1214 318
Cuts at: 4682 5444 5705 5817
Size: 762 261 112
Fragments arranged by size:
1712 1214 762 755 318 294 261 218
139 112 32
Bsu36I CC'TnA_GG
Cuts at: 0 128 1727 3586 4930 5817
Size: 128 1599 1859 1344 887
Fragments arranged by size:
1859 1599 1344 887 128
BtgI C'CryG_G
Cuts at: 0 1763 2060 3572 5817
Size: 1763 297 1512 2245
Fragments arranged by size:
2245 1763 1512 297
BtsI GCAGTG_nn'
Cuts at: 0 1283 5817
Size: 1283 4534
ClaI AT'CG_AT
Cuts at: 0 2219 3695 5817
Size: 2219 1476 2122
Fragments arranged by size:
2219 2122 1476
DraIII CAC_nnn'GTG
Cuts at: 0 2019 5817
Size: 2019 3798
EaeI y'GGCC_r
Cuts at: 0 20 272 1289 2558 2894 3578 3611 5817
Size: 20 252 1017 1269 336 684 33 2206
Fragments arranged by size:
2206 1269 1017 684 336 252 33 20
EarI CTCTTCn'nnn_
Cuts at: 0 983 1607 1966 2427 2588 2795 2824 2945
Size: 983 624 359 461 161 207 29 121
Cuts at: 2945 3667 3794 3965 4667 4765 5261 5773 5807
Size: 722 127 171 702 98 496 512 34
Cuts at: 5807 5817
Size: 10
Fragments arranged by size:
983 722 702 624 512 496 461 359
207 171 161 127 121 98 34 29
10
EcoICRI GAG'CTC
Cuts at: 0 1575 5127 5817
Size: 1575 3552 690
Fragments arranged by size:
3552 1575 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 709 832 1019 1163 1447 1516 1947
Size: 218 491 123 187 144 284 69 431
Cuts at: 1947 2219 2225 2662 2711 2860 2992 3385 3460
Size: 272 6 437 49 149 132 393 75
Cuts at: 3460 3565 3682 4189 4258 4615 5032 5221 5338
Size: 105 117 507 69 357 417 189 117
Cuts at: 5338 5368 5446 5476 5587 5678 5740 5817
Size: 30 78 30 111 91 62 77
Fragments arranged by size:
507 491 437 431 417 393 357 284
272 218 189 187 149 144 132 123
117 117 111 105 91 78 77 75
69 69 62 49 30 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 1612 1715 3547 5053 5817
Size: 688 924 103 1832 1506 764
Fragments arranged by size:
1832 1506 924 764 688 103
EcoO109I rG'GnC_Cy
Cuts at: 0 450 1803 3540 4290 4926 5007 5385 5607
Size: 450 1353 1737 750 636 81 378 222
Cuts at: 5607 5817
Size: 210
Fragments arranged by size:
1737 1353 750 636 450 378 222 210
81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 1332 2332 5817
Size: 153 1179 1000 3485
Fragments arranged by size:
3485 1179 1000 153
FspI TGC'GCA
Cuts at: 0 3535 4690 5817
Size: 3535 1155 1127
Fragments arranged by size:
3535 1155 1127
HaeII r_GCGC'y
Cuts at: 0 327 444 3420 5342 5817
Size: 327 117 2976 1922 475
Fragments arranged by size:
2976 1922 475 327 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 502 534 715 747 1037 1069
Size: 270 32 200 32 181 32 290 32
Cuts at: 1069 1845 1877 2201 2233 2426 2458 2946 2978
Size: 776 32 324 32 193 32 488 32
Cuts at: 2978 4527 4559 5659 5691 5817
Size: 1549 32 1100 32 126
Fragments arranged by size:
1549 1100 776 488 324 290 270 200
193 181 126 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 378 3063 5685 5763 5817
Size: 378 2685 2622 78 54
Fragments arranged by size:
2685 2622 378 78 54
HindIII A'AGCT_T
Cuts at: 0 3247 4415 5817
Size: 3247 1168 1402
Fragments arranged by size:
3247 1402 1168
KpnI G_GTAC'C
Cuts at: 0 770 5817
Size: 770 5047
MfeI C'AATT_G
Cuts at: 0 593 5817
Size: 593 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 5702 5817
Size: 32 5670 115
Fragments arranged by size:
5670 115 32
MscI TGG'CCA
Cuts at: 0 22 274 1291 2560 2896 5817
Size: 22 252 1017 1269 336 2921
Fragments arranged by size:
2921 1269 1017 336 252 22
MslI CAynn'nnrTG
Cuts at: 0 274 1489 2479 4028 4982 5817
Size: 274 1215 990 1549 954 835
Fragments arranged by size:
1549 1215 990 954 835 274
MspA1I CmG'CkG
Cuts at: 0 1347 1434 2088 2361 3321 3813 4896 5022
Size: 1347 87 654 273 960 492 1083 126
Cuts at: 5022 5418 5817
Size: 396 399
Fragments arranged by size:
1347 1083 960 654 492 399 396 273
126 87
NaeI GCC'GGC
Cuts at: 0 1782 5817
Size: 1782 4035
NcoI C'CATG_G
Cuts at: 0 2060 5817
Size: 2060 3757
NgoMIV G'CCGG_C
Cuts at: 0 1780 5817
Size: 1780 4037
NsiI A_TGCA'T
Cuts at: 0 1082 5817
Size: 1082 4735
NspI r_CATG'y
Cuts at: 0 1488 1626 2920 3601 4006 4222 5817
Size: 1488 138 1294 681 405 216 1595
Fragments arranged by size:
1595 1488 1294 681 405 216 138
PciI A'CATG_T
Cuts at: 0 1484 4218 5817
Size: 1484 2734 1599
Fragments arranged by size:
2734 1599 1484
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 2421 4587 5454 5610 5817
Size: 1347 1074 2166 867 156 207
Fragments arranged by size:
2166 1347 1074 867 207 156
PfoI T'CCnGG_A
Cuts at: 0 1607 3008 5129 5246 5603 5817
Size: 1607 1401 2121 117 357 214
Fragments arranged by size:
2121 1607 1401 357 214 117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1519 1551 1609 1641 3505 3537 5111 5143
Size: 1519 32 58 32 1864 32 1574 32
Cuts at: 5143 5817
Size: 674
Fragments arranged by size:
1864 1574 1519 674 58 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 1803 3540 4290 5007 5385 5607 5817
Size: 1803 1737 750 717 378 222 210
Fragments arranged by size:
1803 1737 750 717 378 222 210
PshAI GACnn'nnGTC
Cuts at: 0 109 5817
Size: 109 5708
PstI C_TGCA'G
Cuts at: 0 1188 2128 3044 3326 3644 4013 4205 5057
Size: 1188 940 916 282 318 369 192 852
Cuts at: 5057 5393 5817
Size: 336 424
Fragments arranged by size:
1188 940 916 852 424 369 336 318
282 192
PvuII CAG'CTG
Cuts at: 0 1347 1434 2088 2361 3321 4896 5022 5418
Size: 1347 87 654 273 960 1575 126 396
Cuts at: 5418 5817
Size: 399
Fragments arranged by size:
1575 1347 960 654 399 396 273 126
87
SacI G_AGCT'C
Cuts at: 0 1577 5129 5817
Size: 1577 3552 688
Fragments arranged by size:
3552 1577 688
SapI GCTCTTCn'nnn_
Cuts at: 0 2588 5773 5817
Size: 2588 3185 44
Fragments arranged by size:
3185 2588 44
SbfI CC_TGCA'GG
Cuts at: 0 3044 5817
Size: 3044 2773
ScaI AGT'ACT
Cuts at: 0 583 1372 5817
Size: 583 789 4445
Fragments arranged by size:
4445 789 583
SexAI A'CCwGG_T
Cuts at: 0 5609 5817
Size: 5609 208
SfcI C'TryA_G
Cuts at: 0 1001 1184 2124 3040 3322 3559 3640 4009
Size: 1001 183 940 916 282 237 81 369
Cuts at: 4009 4081 4201 4938 5053 5389 5817
Size: 72 120 737 115 336 428
Fragments arranged by size:
1001 940 916 737 428 369 336 282
237 183 120 115 81 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3397 5281 5817
Size: 3397 1884 536
Fragments arranged by size:
3397 1884 536
SmaI CCC'GGG
Cuts at: 0 3397 3433 5817
Size: 3397 36 2384
Fragments arranged by size:
3397 2384 36
SmlI C'TyrA_G
Cuts at: 0 1995 2860 2881 3197 3915 4252 4699 4852
Size: 1995 865 21 316 718 337 447 153
Cuts at: 4852 4960 5817
Size: 108 857
Fragments arranged by size:
1995 865 857 718 447 337 316 153
108 21
SpeI A'CTAG_T
Cuts at: 0 3900 5817
Size: 3900 1917
SphI G_CATG'C
Cuts at: 0 3601 5817
Size: 3601 2216
SspI AAT'ATT
Cuts at: 0 3871 5592 5817
Size: 3871 1721 225
Fragments arranged by size:
3871 1721 225
StuI AGG'CCT
Cuts at: 0 694 5817
Size: 694 5123
StyI C'CwwG_G
Cuts at: 0 1213 1631 2060 2294 2897 2990 3309 3734
Size: 1213 418 429 234 603 93 319 425
Cuts at: 3734 3794 4070 4097 5817
Size: 60 276 27 1720
Fragments arranged by size:
1720 1213 603 429 425 418 319 276
234 93 60 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 1270 2287 2623 4963 5179 5817
Size: 205 1065 1017 336 2340 216 638
Fragments arranged by size:
2340 1065 1017 638 336 216 205
TatI w'GTAC_w
Cuts at: 0 308 581 1268 1370 1508 2456 5817
Size: 308 273 687 102 138 948 3361
Fragments arranged by size:
3361 948 687 308 273 138 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 1420 1452 1603 1635 5817
Size: 451 32 937 32 151 32 4182
Fragments arranged by size:
4182 937 451 151 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 226 1950 3058 5817
Size: 226 1724 1108 2759
Fragments arranged by size:
2759 1724 1108 226
XbaI T'CTAG_A
Cuts at: 0 820 3153 3765 5817
Size: 820 2333 612 2052
Fragments arranged by size:
2333 2052 820 612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 777 1020 2281 2595 3993 4185 5196
Size: 684 93 243 1261 314 1398 192 1011
Cuts at: 5196 5343 5817
Size: 147 474
Fragments arranged by size:
1398 1261 1011 684 474 314 243 192
147 93
XhoI C'TCGA_G
Cuts at: 0 3915 5817
Size: 3915 1902
XmaI C'CCGG_G
Cuts at: 0 3395 3431 5817
Size: 3395 36 2386
Fragments arranged by size:
3395 2386 36
XmnI GAAnn'nnTTC
Cuts at: 0 2134 5817
Size: 2134 3683
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AloI AlwNI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PpiI PpuMI PshAI PstI
PvuII SacI SapI SbfI ScaI SexAI SfcI SfiI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI ApaI
ApaLI AscI AseI AsiSI BbeI BmgBI BmtI BsaAI
BsaHI BsiEI BsiWI BsmBI BstBI BstZ17I BtgZI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI
HpaI KasI MluI NarI NdeI NheI NotI NruI
PacI PasI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SfoI SgfI SgrAI
SnaBI SrfI SwaI ZraI