Restriction map for
pF1KE2603
(Linear) (Six-base) MAPSORT of: KIEE2603.seq Check: 6318 from: 1 to: 2937
KIEE2603.orf
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 971 1697 2296 2825 2843 2937
Size: 754 217 726 599 529 18 94
Fragments arranged by size:
754 726 599 529 217 94 18
AflIII A'CryG_T
Cuts at: 0 1514 2937
Size: 1514 1423
AlwNI CAG_nnn'CTG
Cuts at: 0 622 1858 1921 2821 2937
Size: 622 1236 63 900 116
Fragments arranged by size:
1236 900 622 116 63
ApaLI G'TGCA_C
Cuts at: 0 2429 2937
Size: 2429 508
ApoI r'AATT_y
Cuts at: 0 304 2937
Size: 304 2633
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 965 998 2937
Size: 965 33 1939
Fragments arranged by size:
1939 965 33
BamHI G'GATC_C
Cuts at: 0 2695 2937
Size: 2695 242
BanI G'GyrC_C
Cuts at: 0 750 2830 2937
Size: 750 2080 107
Fragments arranged by size:
2080 750 107
BanII G_rGCy'C
Cuts at: 0 1957 2937
Size: 1957 980
BbeI G_GCGC'C
Cuts at: 0 2834 2937
Size: 2834 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 2746 2937
Size: 742 2004 191
Fragments arranged by size:
2004 742 191
BbvCI CC'TCA_GC
Cuts at: 0 638 2937
Size: 638 2299
BfrBI ATG'CAT
Cuts at: 0 25 2937
Size: 25 2912
BglI GCCn_nnn'nGGC
Cuts at: 0 2169 2858 2937
Size: 2169 689 79
Fragments arranged by size:
2169 689 79
BglII A'GATC_T
Cuts at: 0 1873 2937
Size: 1873 1064
BlpI GC'TnA_GC
Cuts at: 0 2239 2937
Size: 2239 698
Bme1580I G_kGCm'C
Cuts at: 0 2433 2937
Size: 2433 504
BmgBI CAC'GTC
Cuts at: 0 1791 2937
Size: 1791 1146
BmrI ACTGGGnnnn_n'
Cuts at: 0 1414 2937
Size: 1414 1523
BmtI G_CTAG'C
Cuts at: 0 667 2937
Size: 667 2270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1088 1166 2937
Size: 1088 78 1771
Fragments arranged by size:
1771 1088 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 450 1553 1595 1854 2348 2555 2937
Size: 26 424 1103 42 259 494 207 382
Fragments arranged by size:
1103 494 424 382 259 207 42 26
Bpu10I CC'TnA_GC
Cuts at: 0 386 638 2937
Size: 386 252 2299
Fragments arranged by size:
2299 386 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2073 2937
Size: 2073 864
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 2589 2937
Size: 299 2290 348
Fragments arranged by size:
2290 348 299
BsaHI Gr'CG_yC
Cuts at: 0 2831 2937
Size: 2831 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 230 260 279 309 1551 1581
Size: 107 30 93 30 19 30 1242 30
Cuts at: 1581 1650 1680 2250 2280 2624 2654 2720 2750
Size: 69 30 570 30 344 30 66 30
Cuts at: 2750 2937
Size: 187
Fragments arranged by size:
1242 570 344 187 107 93 69 66
30 30 30 30 30 30 30 30
19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 626 2345 2577 2648 2756 2809 2937
Size: 626 1719 232 71 108 53 128
Fragments arranged by size:
1719 626 232 128 108 71 53
BseYI C'CCAG_C
Cuts at: 0 1029 1215 1647 1863 2137 2218 2788 2937
Size: 1029 186 432 216 274 81 570 149
Fragments arranged by size:
1029 570 432 274 216 186 149 81
BsiHKAI G_wGCw'C
Cuts at: 0 202 1891 2071 2433 2937
Size: 202 1689 180 362 504
Fragments arranged by size:
1689 504 362 202 180
Bsp1286I G_dGCh'C
Cuts at: 0 202 1891 1957 2071 2433 2937
Size: 202 1689 66 114 362 504
Fragments arranged by size:
1689 504 362 202 114 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2016 2451 2730 2937
Size: 2016 435 279 207
Fragments arranged by size:
2016 435 279 207
BsrBI CCG'CTC
Cuts at: 0 2288 2937
Size: 2288 649
BsrDI GCAATG_nn'
Cuts at: 0 74 1805 2937
Size: 74 1731 1132
Fragments arranged by size:
1731 1132 74
BsrFI r'CCGG_y
Cuts at: 0 2413 2799 2937
Size: 2413 386 138
Fragments arranged by size:
2413 386 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2731 2937
Size: 2731 206
BstEII G'GTnAC_C
Cuts at: 0 2884 2937
Size: 2884 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 999 1107 1185 1227 1473 2937
Size: 789 210 108 78 42 246 1464
Fragments arranged by size:
1464 789 246 210 108 78 42
BstYI r'GATC_y
Cuts at: 0 1873 2695 2918 2937
Size: 1873 822 223 19
Fragments arranged by size:
1873 822 223 19
Bsu36I CC'TnA_GG
Cuts at: 0 560 2937
Size: 560 2377
BtgI C'CryG_G
Cuts at: 0 884 2303 2937
Size: 884 1419 634
Fragments arranged by size:
1419 884 634
BtsI GCAGTG_nn'
Cuts at: 0 1351 1982 2937
Size: 1351 631 955
Fragments arranged by size:
1351 955 631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1659 1694 2610 2645 2937
Size: 1659 35 916 35 292
Fragments arranged by size:
1659 916 292 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2034 2937
Size: 2034 903
EaeI y'GGCC_r
Cuts at: 0 355 863 1337 1463 2210 2249 2801 2859
Size: 355 508 474 126 747 39 552 58
Cuts at: 2859 2937
Size: 78
Fragments arranged by size:
747 552 508 474 355 126 78 58
39
EarI CTCTTCn'nnn_
Cuts at: 0 308 686 2937
Size: 308 378 2251
Fragments arranged by size:
2251 378 308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 450 754 971 1553 1595 1697 1854
Size: 26 424 304 217 582 42 102 157
Cuts at: 1854 2296 2348 2555 2825 2843 2937
Size: 442 52 207 270 18 94
Fragments arranged by size:
582 442 424 304 270 217 207 157
102 94 52 42 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 907 949 1633 1761 2330 2630 2726
Size: 781 126 42 684 128 569 300 96
Cuts at: 2726 2937
Size: 211
Fragments arranged by size:
781 684 569 300 211 128 126 96
42
EcoO109I rG'GnC_Cy
Cuts at: 0 1644 2779 2937
Size: 1644 1135 158
Fragments arranged by size:
1644 1135 158
EcoRI G'AATT_C
Cuts at: 0 304 2937
Size: 304 2633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 87 2682 2937
Size: 87 2595 255
Fragments arranged by size:
2595 255 87
HaeII r_GCGC'y
Cuts at: 0 2821 2834 2937
Size: 2821 13 103
Fragments arranged by size:
2821 103 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 820 852 1054 1086 1132 1164
Size: 214 32 574 32 202 32 46 32
Cuts at: 1164 1258 1290 1551 1583 1623 1655 2624 2637
Size: 94 32 261 32 40 32 969 13
Cuts at: 2637 2656 2669 2937
Size: 19 13 268
Fragments arranged by size:
969 574 268 261 214 202 94 46
40 32 32 32 32 32 32 32
19 13 13
KasI G'GCGC_C
Cuts at: 0 2830 2937
Size: 2830 107
MfeI C'AATT_G
Cuts at: 0 2420 2937
Size: 2420 517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 322 405 2937
Size: 105 217 83 2532
Fragments arranged by size:
2532 217 105 83
MscI TGG'CCA
Cuts at: 0 357 865 1339 2251 2861 2937
Size: 357 508 474 912 610 76
Fragments arranged by size:
912 610 508 474 357 76
MslI CAynn'nnrTG
Cuts at: 0 437 2158 2937
Size: 437 1721 779
Fragments arranged by size:
1721 779 437
MspA1I CmG'CkG
Cuts at: 0 478 550 1729 1918 2047 2122 2368 2937
Size: 478 72 1179 189 129 75 246 569
Fragments arranged by size:
1179 569 478 246 189 129 75 72
NaeI GCC'GGC
Cuts at: 0 2415 2801 2937
Size: 2415 386 136
Fragments arranged by size:
2415 386 136
NarI GG'CG_CC
Cuts at: 0 2831 2937
Size: 2831 106
NcoI C'CATG_G
Cuts at: 0 884 2937
Size: 884 2053
NgoMIV G'CCGG_C
Cuts at: 0 2413 2799 2937
Size: 2413 386 138
Fragments arranged by size:
2413 386 138
NheI G'CTAG_C
Cuts at: 0 663 2937
Size: 663 2274
NsiI A_TGCA'T
Cuts at: 0 27 2937
Size: 27 2910
NspI r_CATG'y
Cuts at: 0 876 1518 2530 2937
Size: 876 642 1012 407
Fragments arranged by size:
1012 876 642 407
PciI A'CATG_T
Cuts at: 0 1514 2937
Size: 1514 1423
PflMI CCAn_nnn'nTGG
Cuts at: 0 2907 2937
Size: 2907 30
PfoI T'CCnGG_A
Cuts at: 0 149 2921 2937
Size: 149 2772 16
Fragments arranged by size:
2772 149 16
PpuMI rG'GwC_Cy
Cuts at: 0 1644 2779 2937
Size: 1644 1135 158
Fragments arranged by size:
1644 1135 158
PshAI GACnn'nnGTC
Cuts at: 0 11 2054 2937
Size: 11 2043 883
Fragments arranged by size:
2043 883 11
PstI C_TGCA'G
Cuts at: 0 2013 2127 2727 2937
Size: 2013 114 600 210
Fragments arranged by size:
2013 600 210 114
PvuII CAG'CTG
Cuts at: 0 478 550 1729 1918 2047 2368 2937
Size: 478 72 1179 189 129 321 569
Fragments arranged by size:
1179 569 478 321 189 129 72
SanDI GG'GwC_CC
Cuts at: 0 2779 2937
Size: 2779 158
SbfI CC_TGCA'GG
Cuts at: 0 2727 2937
Size: 2727 210
SfcI C'TryA_G
Cuts at: 0 287 2009 2123 2481 2723 2937
Size: 287 1722 114 358 242 214
Fragments arranged by size:
1722 358 287 242 214 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2858 2937
Size: 2858 79
SfoI GGC'GCC
Cuts at: 0 2832 2937
Size: 2832 105
SmlI C'TyrA_G
Cuts at: 0 2052 2937
Size: 2052 885
SspI AAT'ATT
Cuts at: 0 491 2520 2937
Size: 491 2029 417
Fragments arranged by size:
2029 491 417
StuI AGG'CCT
Cuts at: 0 97 1767 2937
Size: 97 1670 1170
Fragments arranged by size:
1670 1170 97
StyI C'CwwG_G
Cuts at: 0 668 782 866 884 1034 1100 1178 1220
Size: 668 114 84 18 150 66 78 42
Cuts at: 1220 1382 1466 2291 2510 2687 2906 2937
Size: 162 84 825 219 177 219 31
Fragments arranged by size:
825 668 219 219 177 162 150 114
84 84 78 66 42 31 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 855 1131 1209 1752 2172 2937
Size: 855 276 78 543 420 765
Fragments arranged by size:
855 765 543 420 276 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 2720 2752 2937
Size: 586 32 2102 32 185
Fragments arranged by size:
2102 586 185 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1583 2937
Size: 1583 1354
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PpuMI PshAI PstI
PvuII SanDI SbfI SfcI SfiI SfoI SmlI SspI
StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BcgI BciVI BclI BsaAI BsaBI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII
EagI EciI EcoICRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MluI NdeI NotI NruI PacI
PasI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI